Peniophora sp. CONT
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18912 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165Z9K0|A0A165Z9K0_9AGAM Uncharacterized protein OS=Peniophora sp. CONT OX=1314672 GN=PENSPDRAFT_758779 PE=3 SV=1
MM1 pKa = 7.96 ASLLPLLLLALRR13 pKa = 11.84 AHH15 pKa = 7.86 AYY17 pKa = 7.2 TSRR20 pKa = 11.84 HH21 pKa = 4.15 VTFASDD27 pKa = 4.43 DD28 pKa = 3.68 IEE30 pKa = 4.63 KK31 pKa = 9.38 RR32 pKa = 11.84 TALPVAQHH40 pKa = 5.69 VLSLSRR46 pKa = 11.84 TVPRR50 pKa = 11.84 PASKK54 pKa = 10.74 AKK56 pKa = 10.42 ALRR59 pKa = 11.84 HH60 pKa = 4.55 LTNRR64 pKa = 11.84 PEE66 pKa = 4.42 AKK68 pKa = 9.79 NSTVVSVSGSDD79 pKa = 3.17 QDD81 pKa = 3.7 EE82 pKa = 4.69 EE83 pKa = 4.91 YY84 pKa = 11.07 LVDD87 pKa = 3.39 ITFGTQTFPVIVDD100 pKa = 3.86 TGSSDD105 pKa = 2.77 TWLVAKK111 pKa = 9.75 GFQCISSSGDD121 pKa = 3.44 DD122 pKa = 3.9 EE123 pKa = 5.14 PEE125 pKa = 4.11 SEE127 pKa = 4.82 CDD129 pKa = 3.89 FGSSGYY135 pKa = 10.37 DD136 pKa = 3.17 PNVSSTFVAYY146 pKa = 9.46 PGHH149 pKa = 6.45 NFNIAYY155 pKa = 10.11 GDD157 pKa = 4.1 GEE159 pKa = 4.71 FLTGDD164 pKa = 3.54 VGFEE168 pKa = 4.06 TVTIGDD174 pKa = 4.15 LSVTSQEE181 pKa = 4.15 VGVVTTAFWFGDD193 pKa = 4.06 SVSSGLMGLCYY204 pKa = 10.03 PGLTSVFNTTDD215 pKa = 3.68 PDD217 pKa = 4.31 DD218 pKa = 5.47 DD219 pKa = 4.37 SSSNNEE225 pKa = 3.68 EE226 pKa = 3.58 YY227 pKa = 11.02 DD228 pKa = 3.51 PFFFTAIKK236 pKa = 9.75 EE237 pKa = 4.49 GAVSQPVFSVALNRR251 pKa = 11.84 GSVAAEE257 pKa = 3.96 EE258 pKa = 4.92 GSVVDD263 pKa = 4.29 PDD265 pKa = 4.12 LGFLAFGGIAPVDD278 pKa = 3.68 VTATSVTVPIQGEE291 pKa = 4.53 QVSSTDD297 pKa = 3.31 SEE299 pKa = 4.69 FFFYY303 pKa = 10.48 TVDD306 pKa = 2.72 IDD308 pKa = 4.85 AYY310 pKa = 10.42 VFPGSDD316 pKa = 3.39 ALSTSGQSILDD327 pKa = 3.5 TGTTLLYY334 pKa = 10.75 LPTPIANALAAAFDD348 pKa = 4.41 PPAVWSDD355 pKa = 3.61 DD356 pKa = 3.33 EE357 pKa = 4.48 GAYY360 pKa = 10.34 LVNCTATAPEE370 pKa = 4.02 FAVVIGGVSFNVTAADD386 pKa = 4.86 LILPIGADD394 pKa = 3.14 EE395 pKa = 5.91 DD396 pKa = 4.49 GNQVCISGHH405 pKa = 6.85 DD406 pKa = 3.7 DD407 pKa = 3.79 GGPDD411 pKa = 3.28 EE412 pKa = 5.72 DD413 pKa = 4.46 GNIFILGDD421 pKa = 3.68 TFLHH425 pKa = 5.75 NVVSTFDD432 pKa = 3.69 LANNTVTLTEE442 pKa = 4.06 RR443 pKa = 11.84 APYY446 pKa = 9.88
Molecular weight: 46.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.897
Patrickios 1.329
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A166I437|A0A166I437_9AGAM Uncharacterized protein OS=Peniophora sp. CONT OX=1314672 GN=PENSPDRAFT_649276 PE=3 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 PTASSFNTTSNSPTSAPTYY21 pKa = 7.27 TLPRR25 pKa = 11.84 FFRR28 pKa = 11.84 SSATAFLTRR37 pKa = 11.84 TSCSRR42 pKa = 11.84 RR43 pKa = 11.84 VATTRR48 pKa = 11.84 STTSSRR54 pKa = 11.84 RR55 pKa = 11.84 SATRR59 pKa = 11.84 PTLLARR65 pKa = 11.84 AIRR68 pKa = 11.84 ALLARR73 pKa = 11.84 SPRR76 pKa = 11.84 VWVPSTPSGSRR87 pKa = 11.84 TPSRR91 pKa = 11.84 RR92 pKa = 11.84 SFLVPRR98 pKa = 4.76
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18907
5
18912
7847246
49
5293
414.9
45.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.765 ± 0.02
1.34 ± 0.007
5.692 ± 0.012
5.782 ± 0.016
3.582 ± 0.01
6.564 ± 0.015
2.64 ± 0.01
4.45 ± 0.013
3.813 ± 0.016
9.605 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.075 ± 0.006
3.064 ± 0.01
6.502 ± 0.024
3.438 ± 0.011
6.758 ± 0.018
8.279 ± 0.023
5.938 ± 0.011
6.545 ± 0.012
1.532 ± 0.006
2.636 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here