Halovirus HCTV-1
Average proteome isoelectric point is 4.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4TP57|R4TP57_9VIRU Uncharacterized protein OS=Halovirus HCTV-1 OX=1273746 GN=138 PE=4 SV=1
MM1 pKa = 7.46 GNADD5 pKa = 4.65 RR6 pKa = 11.84 LPPEE10 pKa = 4.27 DD11 pKa = 4.07 TFPDD15 pKa = 3.91 GFITDD20 pKa = 4.01 TYY22 pKa = 9.63 EE23 pKa = 3.46 ICADD27 pKa = 3.39 ADD29 pKa = 3.91 GFGEE33 pKa = 3.95 MLPRR37 pKa = 11.84 HH38 pKa = 6.17 SSLGDD43 pKa = 2.99 WNTEE47 pKa = 4.07 YY48 pKa = 10.32 EE49 pKa = 4.32 IHH51 pKa = 6.77 SAAQPGSVLTQYY63 pKa = 10.93 MMLLWSMHH71 pKa = 6.53 EE72 pKa = 3.89 GDD74 pKa = 3.84 FWDD77 pKa = 3.74 WRR79 pKa = 11.84 CDD81 pKa = 3.64 DD82 pKa = 3.99 GVV84 pKa = 3.27
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.821
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|R4TAA2|R4TAA2_9VIRU Uncharacterized protein OS=Halovirus HCTV-1 OX=1273746 GN=7 PE=4 SV=1
MM1 pKa = 7.95 PNRR4 pKa = 11.84 SQSSISEE11 pKa = 4.58 DD12 pKa = 2.86 IRR14 pKa = 11.84 EE15 pKa = 4.02 RR16 pKa = 11.84 RR17 pKa = 11.84 LLSTFTRR24 pKa = 11.84 LLTIMAHH31 pKa = 6.43 PGVLYY36 pKa = 10.8 NVGEE40 pKa = 4.21 APRR43 pKa = 11.84 PRR45 pKa = 11.84 VDD47 pKa = 3.18 PCAPASISALVSARR61 pKa = 11.84 VNKK64 pKa = 9.67 VKK66 pKa = 10.86
Molecular weight: 7.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.619
IPC_protein 10.76
Toseland 10.891
ProMoST 11.023
Dawson 10.95
Bjellqvist 10.774
Wikipedia 11.257
Rodwell 10.935
Grimsley 10.994
Solomon 11.228
Lehninger 11.169
Nozaki 10.891
DTASelect 10.774
Thurlkill 10.891
EMBOSS 11.33
Sillero 10.906
Patrickios 10.774
IPC_peptide 11.228
IPC2_peptide 10.101
IPC2.peptide.svr19 8.852
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
160
0
160
32777
35
1210
204.9
23.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.002 ± 0.235
0.961 ± 0.091
9.998 ± 0.282
10.672 ± 0.326
3.353 ± 0.123
7.536 ± 0.25
1.962 ± 0.115
4.061 ± 0.154
3.759 ± 0.128
6.752 ± 0.184
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.474 ± 0.09
3.716 ± 0.129
4.009 ± 0.127
3.319 ± 0.113
5.983 ± 0.193
6.264 ± 0.192
5.818 ± 0.184
7.456 ± 0.137
1.687 ± 0.103
3.219 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here