Pacific flying fox faeces associated circular DNA virus-11
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTV1|A0A140CTV1_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-11 OX=1796006 PE=4 SV=1
MM1 pKa = 7.6 SKK3 pKa = 10.4 PSRR6 pKa = 11.84 NFCFTLNNPDD16 pKa = 3.88 DD17 pKa = 4.72 EE18 pKa = 4.91 YY19 pKa = 11.3 TFFADD24 pKa = 4.35 RR25 pKa = 11.84 FPRR28 pKa = 11.84 FQTQYY33 pKa = 11.43 AIWQLEE39 pKa = 3.78 AGEE42 pKa = 4.35 NGTRR46 pKa = 11.84 DD47 pKa = 3.4 LQGYY51 pKa = 9.72 LEE53 pKa = 4.8 LSSPARR59 pKa = 11.84 VSAFQSTTLKK69 pKa = 10.46 GAHH72 pKa = 5.96 FEE74 pKa = 4.12 PRR76 pKa = 11.84 KK77 pKa = 8.33 GTRR80 pKa = 11.84 AQARR84 pKa = 11.84 DD85 pKa = 3.61 YY86 pKa = 10.97 CRR88 pKa = 11.84 KK89 pKa = 10.0 LDD91 pKa = 3.73 TRR93 pKa = 11.84 VDD95 pKa = 4.15 GPWEE99 pKa = 4.03 YY100 pKa = 10.5 GTFGAGGQGRR110 pKa = 11.84 RR111 pKa = 11.84 SDD113 pKa = 3.93 LEE115 pKa = 4.03 AVEE118 pKa = 4.47 QSIKK122 pKa = 10.55 RR123 pKa = 11.84 GASLAEE129 pKa = 3.83 IAEE132 pKa = 4.18 EE133 pKa = 4.19 HH134 pKa = 5.18 TTSFIKK140 pKa = 10.31 YY141 pKa = 9.02 ARR143 pKa = 11.84 NISLTCEE150 pKa = 4.1 ILCPTTPRR158 pKa = 11.84 NPEE161 pKa = 4.09 TPCEE165 pKa = 3.81 VHH167 pKa = 6.93 LYY169 pKa = 9.99 HH170 pKa = 7.22 GPSGCGKK177 pKa = 8.06 TRR179 pKa = 11.84 AITADD184 pKa = 3.45 SLLQGSSVYY193 pKa = 10.05 WKK195 pKa = 10.82 DD196 pKa = 3.24 NGKK199 pKa = 8.16 WWDD202 pKa = 3.73 GYY204 pKa = 10.13 RR205 pKa = 11.84 GHH207 pKa = 7.12 TIVVADD213 pKa = 4.46 DD214 pKa = 3.82 FSGASMRR221 pKa = 11.84 YY222 pKa = 8.67 CDD224 pKa = 3.81 FRR226 pKa = 11.84 RR227 pKa = 11.84 WFDD230 pKa = 3.61 RR231 pKa = 11.84 YY232 pKa = 8.57 PCRR235 pKa = 11.84 VEE237 pKa = 4.71 RR238 pKa = 11.84 KK239 pKa = 9.83 GDD241 pKa = 3.54 TTEE244 pKa = 4.34 LVATRR249 pKa = 11.84 FYY251 pKa = 10.73 ISSTKK256 pKa = 10.65 LPDD259 pKa = 3.37 LWWDD263 pKa = 3.31 RR264 pKa = 11.84 SVVKK268 pKa = 10.05 EE269 pKa = 3.98 YY270 pKa = 11.06 NWMEE274 pKa = 3.55 IARR277 pKa = 11.84 RR278 pKa = 11.84 ITHH281 pKa = 6.2 FHH283 pKa = 6.15 TFDD286 pKa = 3.52 EE287 pKa = 4.8 EE288 pKa = 4.48 TKK290 pKa = 10.41 HH291 pKa = 5.19 LHH293 pKa = 6.38 SYY295 pKa = 9.46 TGTDD299 pKa = 2.64 QATALDD305 pKa = 4.28 QYY307 pKa = 10.77 CDD309 pKa = 3.65 SINYY313 pKa = 7.57 YY314 pKa = 10.43 HH315 pKa = 6.76 YY316 pKa = 11.5
Molecular weight: 36.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.096
IPC2_protein 6.135
IPC_protein 6.237
Toseland 6.453
ProMoST 6.664
Dawson 6.59
Bjellqvist 6.561
Wikipedia 6.605
Rodwell 6.576
Grimsley 6.561
Solomon 6.59
Lehninger 6.59
Nozaki 6.868
DTASelect 7.015
Thurlkill 7.044
EMBOSS 7.029
Sillero 6.985
Patrickios 3.884
IPC_peptide 6.605
IPC2_peptide 6.868
IPC2.peptide.svr19 6.78
Protein with the highest isoelectric point:
>tr|A0A140CTV1|A0A140CTV1_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-11 OX=1796006 PE=4 SV=1
MM1 pKa = 7.36 RR2 pKa = 11.84 RR3 pKa = 11.84 NYY5 pKa = 9.85 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 SRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 SRR14 pKa = 11.84 HH15 pKa = 4.28 HH16 pKa = 6.16 SKK18 pKa = 11.03 YY19 pKa = 10.5 NEE21 pKa = 4.03 SQVFSITRR29 pKa = 11.84 KK30 pKa = 6.99 TWGTYY35 pKa = 8.28 IQNTASNSTFWAGSLNASYY54 pKa = 11.38 AKK56 pKa = 9.85 TFTLGDD62 pKa = 3.61 VPAVTEE68 pKa = 4.05 FTNLFEE74 pKa = 4.72 EE75 pKa = 4.42 YY76 pKa = 9.43 RR77 pKa = 11.84 IVGIRR82 pKa = 11.84 VTVNLDD88 pKa = 2.96 TGINVQPTLDD98 pKa = 3.51 YY99 pKa = 8.76 GTWFMWVDD107 pKa = 3.48 NDD109 pKa = 6.15 DD110 pKa = 4.03 GTQLDD115 pKa = 4.4 PTTGSNFDD123 pKa = 3.97 LVLQRR128 pKa = 11.84 GRR130 pKa = 11.84 VKK132 pKa = 10.12 TFNGNDD138 pKa = 4.08 GKK140 pKa = 10.92 RR141 pKa = 11.84 HH142 pKa = 4.66 TMWVKK147 pKa = 9.89 PMVSAEE153 pKa = 4.07 YY154 pKa = 9.81 YY155 pKa = 9.33 RR156 pKa = 11.84 ASGGVNAYY164 pKa = 9.37 GPKK167 pKa = 9.24 YY168 pKa = 10.54 SPWLSTSNTGTPHH181 pKa = 6.2 YY182 pKa = 10.45 SFKK185 pKa = 10.34 IWVRR189 pKa = 11.84 NMTSNILGFNIMCKK203 pKa = 10.26 YY204 pKa = 8.98 YY205 pKa = 10.18 LQFRR209 pKa = 11.84 GVQQ212 pKa = 3.08
Molecular weight: 24.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.726
IPC2_protein 9.706
IPC_protein 10.189
Toseland 9.926
ProMoST 9.823
Dawson 10.233
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.409
Grimsley 10.35
Solomon 10.262
Lehninger 10.218
Nozaki 9.926
DTASelect 9.984
Thurlkill 10.058
EMBOSS 10.365
Sillero 10.145
Patrickios 5.347
IPC_peptide 10.262
IPC2_peptide 8.858
IPC2.peptide.svr19 8.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
528
212
316
264.0
30.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.682 ± 1.067
1.894 ± 0.796
5.871 ± 0.909
4.735 ± 1.329
5.114 ± 0.042
7.386 ± 0.354
2.652 ± 0.428
3.788 ± 0.008
4.167 ± 0.044
5.682 ± 0.276
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.705 ± 0.63
4.924 ± 1.731
3.788 ± 0.272
3.409 ± 0.06
8.523 ± 0.018
7.386 ± 0.354
9.47 ± 0.508
4.924 ± 1.203
3.03 ± 0.152
5.871 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here