Citrus chlorotic spot virus
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6AWK5|A0A1W6AWK5_9RHAB GDP polyribonucleotidyltransferase OS=Citrus chlorotic spot virus OX=1980624 GN=ORF6 PE=4 SV=1
MM1 pKa = 7.38 ARR3 pKa = 11.84 YY4 pKa = 9.95 ADD6 pKa = 3.42 VDD8 pKa = 3.91 YY9 pKa = 11.07 TSVLDD14 pKa = 4.22 IYY16 pKa = 11.15 SDD18 pKa = 3.82 TPEE21 pKa = 4.1 EE22 pKa = 4.22 SQVASSAPTPVTYY35 pKa = 10.59 SRR37 pKa = 11.84 EE38 pKa = 3.99 AAVAKK43 pKa = 10.04 PIVTLEE49 pKa = 4.24 APPDD53 pKa = 3.47 THH55 pKa = 8.51 ADD57 pKa = 3.7 VITLFQTIMDD67 pKa = 4.4 HH68 pKa = 6.67 ANSRR72 pKa = 11.84 MTKK75 pKa = 10.03 KK76 pKa = 10.42 DD77 pKa = 3.4 LLVIMSLGFCLTPPGCEE94 pKa = 4.11 GPSMWSVAQGAVVQPLSMPGITAVYY119 pKa = 10.03 DD120 pKa = 3.49 SRR122 pKa = 11.84 AAAASSTMNIDD133 pKa = 3.59 PDD135 pKa = 3.83 EE136 pKa = 4.93 GDD138 pKa = 3.35 QAEE141 pKa = 4.87 DD142 pKa = 3.45 SQAPPDD148 pKa = 4.59 DD149 pKa = 4.12 EE150 pKa = 5.79 DD151 pKa = 4.41 AASQCRR157 pKa = 11.84 AISYY161 pKa = 9.84 ICLSLMRR168 pKa = 11.84 LAVKK172 pKa = 10.22 RR173 pKa = 11.84 VEE175 pKa = 4.28 TFMKK179 pKa = 9.07 GTPQLVTAYY188 pKa = 9.41 HH189 pKa = 6.84 ILMGDD194 pKa = 3.88 HH195 pKa = 6.95 SPFLASFNYY204 pKa = 9.9 SRR206 pKa = 11.84 GLCSNISSLFNQCDD220 pKa = 3.53 DD221 pKa = 3.59 MKK223 pKa = 10.53 RR224 pKa = 11.84 TLAHH228 pKa = 6.13 HH229 pKa = 6.47 CAVADD234 pKa = 4.21 EE235 pKa = 4.59 SHH237 pKa = 6.51 HH238 pKa = 6.25 NNRR241 pKa = 11.84 TVHH244 pKa = 5.82 GPLRR248 pKa = 11.84 FLILQHH254 pKa = 6.25 MDD256 pKa = 3.32 LNGMVPYY263 pKa = 10.95 GMYY266 pKa = 10.71 VDD268 pKa = 4.28 MKK270 pKa = 10.73 RR271 pKa = 11.84 GLPLLKK277 pKa = 10.13 PGTILTWLHH286 pKa = 6.46 DD287 pKa = 4.11 AQVASTLMLISKK299 pKa = 9.74 INKK302 pKa = 9.17 DD303 pKa = 3.07 HH304 pKa = 7.64 DD305 pKa = 3.87 RR306 pKa = 11.84 TDD308 pKa = 3.09 KK309 pKa = 11.19 ADD311 pKa = 3.88 RR312 pKa = 11.84 FWRR315 pKa = 11.84 YY316 pKa = 8.93 CRR318 pKa = 11.84 CIDD321 pKa = 3.38 PGFFIEE327 pKa = 4.58 LQQSRR332 pKa = 11.84 CYY334 pKa = 10.61 ILIARR339 pKa = 11.84 MADD342 pKa = 2.74 ILVRR346 pKa = 11.84 GGCVNVTEE354 pKa = 4.33 YY355 pKa = 11.35 SDD357 pKa = 3.56 PKK359 pKa = 10.14 KK360 pKa = 10.94 AEE362 pKa = 4.23 SIKK365 pKa = 11.14 DD366 pKa = 3.45 KK367 pKa = 11.68 ANILANAEE375 pKa = 3.95 RR376 pKa = 11.84 FGIEE380 pKa = 4.96 FMAAYY385 pKa = 8.96 NALSGTSEE393 pKa = 4.09 GAGPVARR400 pKa = 11.84 ALASAAVGQPTRR412 pKa = 11.84 RR413 pKa = 11.84 ILVPRR418 pKa = 11.84 NPRR421 pKa = 11.84 PQPTVNVTSNPPPQRR436 pKa = 11.84 VGALDD441 pKa = 5.28 SMIQDD446 pKa = 3.43 QSS448 pKa = 3.21
Molecular weight: 49.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.563
IPC2_protein 5.537
IPC_protein 5.575
Toseland 5.931
ProMoST 5.906
Dawson 5.83
Bjellqvist 5.842
Wikipedia 5.83
Rodwell 5.804
Grimsley 6.046
Solomon 5.83
Lehninger 5.817
Nozaki 6.084
DTASelect 6.287
Thurlkill 6.262
EMBOSS 6.237
Sillero 6.186
Patrickios 3.91
IPC_peptide 5.842
IPC2_peptide 6.186
IPC2.peptide.svr19 6.142
Protein with the highest isoelectric point:
>tr|A0A1W6AWK1|A0A1W6AWK1_9RHAB Movement protein OS=Citrus chlorotic spot virus OX=1980624 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.14 ATSSKK6 pKa = 9.51 STEE9 pKa = 4.1 EE10 pKa = 4.22 LCTVMSVRR18 pKa = 11.84 SRR20 pKa = 11.84 VTLTSPSKK28 pKa = 10.66 PPMDD32 pKa = 4.43 AATIASMVKK41 pKa = 10.23 AIGVMKK47 pKa = 10.6 SKK49 pKa = 10.96 ALTPEE54 pKa = 4.05 GTEE57 pKa = 4.93 VSGDD61 pKa = 3.2 WQALITEE68 pKa = 5.0 VARR71 pKa = 11.84 QSKK74 pKa = 8.75 PQVSGPFLTKK84 pKa = 10.48 DD85 pKa = 3.47 LEE87 pKa = 4.49 SGNIYY92 pKa = 9.21 TINMIIGGPLNVKK105 pKa = 10.0 DD106 pKa = 4.13 NNHH109 pKa = 5.33 GRR111 pKa = 11.84 VMTLRR116 pKa = 11.84 SGPLYY121 pKa = 7.92 DD122 pKa = 3.72 TPHH125 pKa = 6.42 YY126 pKa = 10.7 SSNDD130 pKa = 3.04 KK131 pKa = 10.73 VDD133 pKa = 3.16 IMLSSGKK140 pKa = 10.22 ASVGISTSIATVKK153 pKa = 10.31 RR154 pKa = 11.84 PRR156 pKa = 11.84 SSCEE160 pKa = 3.53 KK161 pKa = 10.51 LVNQYY166 pKa = 10.73 IILPKK171 pKa = 10.57 EE172 pKa = 4.51 KK173 pKa = 9.88 INPPAPRR180 pKa = 11.84 PSGG183 pKa = 3.35
Molecular weight: 19.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.88
IPC2_protein 8.829
IPC_protein 8.77
Toseland 9.663
ProMoST 9.297
Dawson 9.853
Bjellqvist 9.516
Wikipedia 9.984
Rodwell 10.335
Grimsley 9.897
Solomon 9.897
Lehninger 9.882
Nozaki 9.721
DTASelect 9.487
Thurlkill 9.721
EMBOSS 10.058
Sillero 9.78
Patrickios 9.853
IPC_peptide 9.897
IPC2_peptide 8.053
IPC2.peptide.svr19 7.787
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3613
183
1874
602.2
67.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.757 ± 0.804
2.187 ± 0.184
6.061 ± 0.441
4.927 ± 0.335
2.851 ± 0.396
5.729 ± 0.405
3.211 ± 0.375
6.975 ± 0.478
5.84 ± 0.556
8.497 ± 0.488
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.318 ± 0.261
4.484 ± 0.284
4.761 ± 0.737
2.878 ± 0.479
5.369 ± 0.352
9.189 ± 0.542
5.231 ± 0.512
7.002 ± 0.378
1.605 ± 0.241
3.128 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here