Clostridium phage phiCT19406C
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7RWJ1|A0A0A7RWJ1_9CAUD Uncharacterized protein OS=Clostridium phage phiCT19406C OX=1567011 GN=phiCT19406C_14 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.74 NLPDD6 pKa = 3.55 CQYY9 pKa = 10.57 EE10 pKa = 4.18 YY11 pKa = 10.99 GYY13 pKa = 9.44 EE14 pKa = 3.95 APIMQIAEE22 pKa = 3.98 EE23 pKa = 4.25 CSICGHH29 pKa = 6.68 EE30 pKa = 4.3 IYY32 pKa = 10.72 EE33 pKa = 4.21 GDD35 pKa = 3.71 EE36 pKa = 4.32 YY37 pKa = 11.88 YY38 pKa = 10.81 HH39 pKa = 6.98 IDD41 pKa = 3.54 NLKK44 pKa = 9.86 ICEE47 pKa = 3.97 DD48 pKa = 4.59 CILDD52 pKa = 3.72 FKK54 pKa = 11.29 KK55 pKa = 10.4 IGEE58 pKa = 4.07 II59 pKa = 3.91
Molecular weight: 6.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.967
IPC2_protein 3.999
IPC_protein 3.872
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.999
Patrickios 0.006
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|A0A0A7S142|A0A0A7S142_9CAUD Uncharacterized protein OS=Clostridium phage phiCT19406C OX=1567011 GN=phiCT19406C_28 PE=4 SV=1
MM1 pKa = 7.41 CRR3 pKa = 11.84 VDD5 pKa = 3.98 MLLDD9 pKa = 3.6 KK10 pKa = 10.56 ALKK13 pKa = 9.95 RR14 pKa = 11.84 IRR16 pKa = 11.84 VNLRR20 pKa = 11.84 KK21 pKa = 9.52 EE22 pKa = 4.0 FKK24 pKa = 10.18 YY25 pKa = 10.4 KK26 pKa = 10.14 KK27 pKa = 9.94 RR28 pKa = 11.84 CINRR32 pKa = 11.84 NKK34 pKa = 10.31 LYY36 pKa = 10.52 KK37 pKa = 10.07 KK38 pKa = 10.1 RR39 pKa = 11.84 IKK41 pKa = 10.11 QGRR44 pKa = 11.84 KK45 pKa = 9.63 GII47 pKa = 4.02
Molecular weight: 5.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 10.087
IPC_protein 10.994
Toseland 11.316
ProMoST 11.272
Dawson 11.345
Bjellqvist 11.125
Wikipedia 11.623
Rodwell 11.637
Grimsley 11.374
Solomon 11.594
Lehninger 11.55
Nozaki 11.301
DTASelect 11.111
Thurlkill 11.301
EMBOSS 11.74
Sillero 11.301
Patrickios 11.374
IPC_peptide 11.608
IPC2_peptide 10.292
IPC2.peptide.svr19 8.495
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
63
0
63
10835
43
1148
172.0
19.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.095 ± 0.469
1.191 ± 0.171
5.639 ± 0.279
9.599 ± 0.334
3.396 ± 0.24
5.381 ± 0.277
1.108 ± 0.128
8.15 ± 0.417
11.371 ± 0.384
8.519 ± 0.361
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.806 ± 0.188
7.208 ± 0.269
1.957 ± 0.148
3.036 ± 0.209
3.95 ± 0.268
5.371 ± 0.308
5.565 ± 0.344
5.482 ± 0.218
0.978 ± 0.102
4.199 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here