Amino acid dipepetide frequency for Fructobacillus sp. EFB-N1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.632AlaAla: 8.632 ± 0.238
0.258AlaCys: 0.258 ± 0.025
6.299AlaAsp: 6.299 ± 0.147
5.141AlaGlu: 5.141 ± 0.126
3.858AlaPhe: 3.858 ± 0.105
7.11AlaGly: 7.11 ± 0.162
1.485AlaHis: 1.485 ± 0.057
6.449AlaIle: 6.449 ± 0.163
6.662AlaLys: 6.662 ± 0.144
9.375AlaLeu: 9.375 ± 0.166
2.26AlaMet: 2.26 ± 0.08
4.17AlaAsn: 4.17 ± 0.096
2.478AlaPro: 2.478 ± 0.083
4.308AlaGln: 4.308 ± 0.13
3.028AlaArg: 3.028 ± 0.083
4.592AlaSer: 4.592 ± 0.116
5.252AlaThr: 5.252 ± 0.118
6.967AlaVal: 6.967 ± 0.143
0.869AlaTrp: 0.869 ± 0.044
2.99AlaTyr: 2.99 ± 0.109
0.0AlaXaa: 0.0 ± 0.0
Cys
0.149CysAla: 0.149 ± 0.017
0.03CysCys: 0.03 ± 0.008
0.115CysAsp: 0.115 ± 0.015
0.094CysGlu: 0.094 ± 0.016
0.149CysPhe: 0.149 ± 0.021
0.233CysGly: 0.233 ± 0.02
0.075CysHis: 0.075 ± 0.015
0.113CysIle: 0.113 ± 0.016
0.064CysLys: 0.064 ± 0.013
0.352CysLeu: 0.352 ± 0.033
0.058CysMet: 0.058 ± 0.012
0.087CysAsn: 0.087 ± 0.015
0.12CysPro: 0.12 ± 0.015
0.175CysGln: 0.175 ± 0.021
0.126CysArg: 0.126 ± 0.017
0.175CysSer: 0.175 ± 0.021
0.109CysThr: 0.109 ± 0.016
0.111CysVal: 0.111 ± 0.015
0.032CysTrp: 0.032 ± 0.009
0.077CysTyr: 0.077 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
4.479AspAla: 4.479 ± 0.138
0.166AspCys: 0.166 ± 0.02
3.726AspAsp: 3.726 ± 0.103
4.012AspGlu: 4.012 ± 0.102
3.338AspPhe: 3.338 ± 0.087
4.02AspGly: 4.02 ± 0.1
1.361AspHis: 1.361 ± 0.058
3.235AspIle: 3.235 ± 0.091
3.397AspLys: 3.397 ± 0.105
7.369AspLeu: 7.369 ± 0.157
1.417AspMet: 1.417 ± 0.057
2.505AspAsn: 2.505 ± 0.088
2.121AspPro: 2.121 ± 0.067
4.537AspGln: 4.537 ± 0.145
2.746AspArg: 2.746 ± 0.091
3.205AspSer: 3.205 ± 0.094
2.751AspThr: 2.751 ± 0.086
3.929AspVal: 3.929 ± 0.092
0.819AspTrp: 0.819 ± 0.043
2.424AspTyr: 2.424 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
5.053GluAla: 5.053 ± 0.115
0.068GluCys: 0.068 ± 0.011
3.265GluAsp: 3.265 ± 0.106
3.997GluGlu: 3.997 ± 0.117
1.865GluPhe: 1.865 ± 0.061
2.934GluGly: 2.934 ± 0.077
1.001GluHis: 1.001 ± 0.051
3.747GluIle: 3.747 ± 0.11
4.708GluLys: 4.708 ± 0.11
5.26GluLeu: 5.26 ± 0.131
1.654GluMet: 1.654 ± 0.06
3.263GluAsn: 3.263 ± 0.085
1.782GluPro: 1.782 ± 0.067
2.823GluGln: 2.823 ± 0.085
2.377GluArg: 2.377 ± 0.075
2.934GluSer: 2.934 ± 0.074
3.015GluThr: 3.015 ± 0.082
4.031GluVal: 4.031 ± 0.103
0.452GluTrp: 0.452 ± 0.037
1.607GluTyr: 1.607 ± 0.06
0.0GluXaa: 0.0 ± 0.0
Phe
3.845PheAla: 3.845 ± 0.102
0.137PheCys: 0.137 ± 0.019
3.152PheAsp: 3.152 ± 0.087
2.266PheGlu: 2.266 ± 0.08
1.976PhePhe: 1.976 ± 0.078
3.327PheGly: 3.327 ± 0.086
0.781PheHis: 0.781 ± 0.042
2.557PheIle: 2.557 ± 0.088
2.049PheLys: 2.049 ± 0.081
4.332PheLeu: 4.332 ± 0.123
1.022PheMet: 1.022 ± 0.049
1.95PheAsn: 1.95 ± 0.069
1.261PhePro: 1.261 ± 0.052
1.733PheGln: 1.733 ± 0.058
1.295PheArg: 1.295 ± 0.062
2.926PheSer: 2.926 ± 0.089
2.373PheThr: 2.373 ± 0.076
3.131PheVal: 3.131 ± 0.099
0.563PheTrp: 0.563 ± 0.036
1.56PheTyr: 1.56 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
5.403GlyAla: 5.403 ± 0.136
0.169GlyCys: 0.169 ± 0.019
3.662GlyAsp: 3.662 ± 0.098
3.329GlyGlu: 3.329 ± 0.083
3.182GlyPhe: 3.182 ± 0.081
4.37GlyGly: 4.37 ± 0.123
1.62GlyHis: 1.62 ± 0.067
4.712GlyIle: 4.712 ± 0.124
3.961GlyLys: 3.961 ± 0.109
7.27GlyLeu: 7.27 ± 0.149
1.942GlyMet: 1.942 ± 0.065
2.725GlyAsn: 2.725 ± 0.093
1.761GlyPro: 1.761 ± 0.059
4.603GlyGln: 4.603 ± 0.107
2.836GlyArg: 2.836 ± 0.082
3.933GlySer: 3.933 ± 0.115
4.087GlyThr: 4.087 ± 0.103
4.932GlyVal: 4.932 ± 0.117
0.775GlyTrp: 0.775 ± 0.046
2.514GlyTyr: 2.514 ± 0.094
0.0GlyXaa: 0.0 ± 0.0
His
1.317HisAla: 1.317 ± 0.062
0.073HisCys: 0.073 ± 0.013
1.317HisAsp: 1.317 ± 0.055
1.118HisGlu: 1.118 ± 0.048
1.003HisPhe: 1.003 ± 0.048
1.475HisGly: 1.475 ± 0.068
0.585HisHis: 0.585 ± 0.035
0.975HisIle: 0.975 ± 0.048
0.805HisLys: 0.805 ± 0.04
2.004HisLeu: 2.004 ± 0.065
0.429HisMet: 0.429 ± 0.032
0.717HisAsn: 0.717 ± 0.037
0.952HisPro: 0.952 ± 0.051
1.278HisGln: 1.278 ± 0.06
0.798HisArg: 0.798 ± 0.038
0.958HisSer: 0.958 ± 0.046
0.781HisThr: 0.781 ± 0.042
1.283HisVal: 1.283 ± 0.055
0.241HisTrp: 0.241 ± 0.021
0.898HisTyr: 0.898 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
5.792IleAla: 5.792 ± 0.157
0.209IleCys: 0.209 ± 0.021
4.266IleAsp: 4.266 ± 0.085
3.653IleGlu: 3.653 ± 0.11
2.736IlePhe: 2.736 ± 0.087
4.75IleGly: 4.75 ± 0.115
1.142IleHis: 1.142 ± 0.053
3.909IleIle: 3.909 ± 0.119
3.726IleLys: 3.726 ± 0.095
6.244IleLeu: 6.244 ± 0.136
1.376IleMet: 1.376 ± 0.063
3.199IleAsn: 3.199 ± 0.093
2.298IlePro: 2.298 ± 0.074
2.815IleGln: 2.815 ± 0.08
2.341IleArg: 2.341 ± 0.077
3.809IleSer: 3.809 ± 0.096
3.658IleThr: 3.658 ± 0.088
4.895IleVal: 4.895 ± 0.111
0.674IleTrp: 0.674 ± 0.041
1.899IleTyr: 1.899 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
5.634LysAla: 5.634 ± 0.116
0.085LysCys: 0.085 ± 0.013
3.79LysAsp: 3.79 ± 0.095
4.345LysGlu: 4.345 ± 0.109
1.884LysPhe: 1.884 ± 0.077
3.173LysGly: 3.173 ± 0.092
1.125LysHis: 1.125 ± 0.057
4.037LysIle: 4.037 ± 0.104
5.363LysLys: 5.363 ± 0.123
4.812LysLeu: 4.812 ± 0.115
1.968LysMet: 1.968 ± 0.069
3.495LysAsn: 3.495 ± 0.089
2.119LysPro: 2.119 ± 0.072
3.254LysGln: 3.254 ± 0.108
2.785LysArg: 2.785 ± 0.092
3.724LysSer: 3.724 ± 0.083
4.422LysThr: 4.422 ± 0.109
4.321LysVal: 4.321 ± 0.093
0.504LysTrp: 0.504 ± 0.029
1.765LysTyr: 1.765 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
11.359LeuAla: 11.359 ± 0.198
0.198LeuCys: 0.198 ± 0.023
5.83LeuAsp: 5.83 ± 0.133
4.772LeuGlu: 4.772 ± 0.11
3.837LeuPhe: 3.837 ± 0.119
6.387LeuGly: 6.387 ± 0.148
1.577LeuHis: 1.577 ± 0.061
6.468LeuIle: 6.468 ± 0.144
5.886LeuLys: 5.886 ± 0.127
9.334LeuLeu: 9.334 ± 0.213
2.411LeuMet: 2.411 ± 0.067
4.33LeuAsn: 4.33 ± 0.099
4.464LeuPro: 4.464 ± 0.123
4.063LeuGln: 4.063 ± 0.104
3.252LeuArg: 3.252 ± 0.095
7.038LeuSer: 7.038 ± 0.123
7.264LeuThr: 7.264 ± 0.132
7.748LeuVal: 7.748 ± 0.157
0.768LeuTrp: 0.768 ± 0.041
2.533LeuTyr: 2.533 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
2.892MetAla: 2.892 ± 0.08
0.041MetCys: 0.041 ± 0.01
1.329MetAsp: 1.329 ± 0.052
1.118MetGlu: 1.118 ± 0.049
0.772MetPhe: 0.772 ± 0.043
1.645MetGly: 1.645 ± 0.072
0.344MetHis: 0.344 ± 0.028
1.643MetIle: 1.643 ± 0.064
1.697MetLys: 1.697 ± 0.07
1.989MetLeu: 1.989 ± 0.071
0.732MetMet: 0.732 ± 0.046
1.15MetAsn: 1.15 ± 0.05
1.02MetPro: 1.02 ± 0.043
1.054MetGln: 1.054 ± 0.053
0.83MetArg: 0.83 ± 0.041
1.513MetSer: 1.513 ± 0.057
2.14MetThr: 2.14 ± 0.073
2.151MetVal: 2.151 ± 0.075
0.145MetTrp: 0.145 ± 0.019
0.497MetTyr: 0.497 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.513AsnAla: 3.513 ± 0.103
0.126AsnCys: 0.126 ± 0.015
2.608AsnAsp: 2.608 ± 0.075
2.341AsnGlu: 2.341 ± 0.075
2.059AsnPhe: 2.059 ± 0.07
3.527AsnGly: 3.527 ± 0.095
1.116AsnHis: 1.116 ± 0.047
2.531AsnIle: 2.531 ± 0.074
2.601AsnLys: 2.601 ± 0.085
4.3AsnLeu: 4.3 ± 0.093
1.069AsnMet: 1.069 ± 0.055
2.29AsnAsn: 2.29 ± 0.099
2.307AsnPro: 2.307 ± 0.069
3.72AsnGln: 3.72 ± 0.119
2.125AsnArg: 2.125 ± 0.07
2.463AsnSer: 2.463 ± 0.102
2.313AsnThr: 2.313 ± 0.075
2.909AsnVal: 2.909 ± 0.094
0.664AsnTrp: 0.664 ± 0.035
1.554AsnTyr: 1.554 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
3.619ProAla: 3.619 ± 0.095
0.058ProCys: 0.058 ± 0.009
2.606ProAsp: 2.606 ± 0.093
2.512ProGlu: 2.512 ± 0.08
1.539ProPhe: 1.539 ± 0.062
2.399ProGly: 2.399 ± 0.078
0.602ProHis: 0.602 ± 0.038
2.26ProIle: 2.26 ± 0.068
2.422ProLys: 2.422 ± 0.076
3.203ProLeu: 3.203 ± 0.081
0.834ProMet: 0.834 ± 0.042
1.641ProAsn: 1.641 ± 0.065
0.465ProPro: 0.465 ± 0.032
1.253ProGln: 1.253 ± 0.059
1.071ProArg: 1.071 ± 0.055
2.029ProSer: 2.029 ± 0.068
2.281ProThr: 2.281 ± 0.074
3.028ProVal: 3.028 ± 0.084
0.346ProTrp: 0.346 ± 0.024
1.246ProTyr: 1.246 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
5.945GlnAla: 5.945 ± 0.192
0.096GlnCys: 0.096 ± 0.014
3.005GlnAsp: 3.005 ± 0.096
3.468GlnGlu: 3.468 ± 0.11
1.492GlnPhe: 1.492 ± 0.063
3.009GlnGly: 3.009 ± 0.089
0.802GlnHis: 0.802 ± 0.046
3.316GlnIle: 3.316 ± 0.1
3.698GlnLys: 3.698 ± 0.099
4.588GlnLeu: 4.588 ± 0.11
1.169GlnMet: 1.169 ± 0.054
2.571GlnAsn: 2.571 ± 0.079
1.81GlnPro: 1.81 ± 0.074
2.746GlnGln: 2.746 ± 0.109
1.846GlnArg: 1.846 ± 0.073
3.205GlnSer: 3.205 ± 0.108
3.444GlnThr: 3.444 ± 0.102
4.375GlnVal: 4.375 ± 0.105
0.444GlnTrp: 0.444 ± 0.03
1.477GlnTyr: 1.477 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.926ArgAla: 2.926 ± 0.089
0.098ArgCys: 0.098 ± 0.015
2.128ArgAsp: 2.128 ± 0.07
2.258ArgGlu: 2.258 ± 0.079
1.972ArgPhe: 1.972 ± 0.075
2.403ArgGly: 2.403 ± 0.093
0.901ArgHis: 0.901 ± 0.045
2.241ArgIle: 2.241 ± 0.071
2.061ArgLys: 2.061 ± 0.074
4.375ArgLeu: 4.375 ± 0.106
0.962ArgMet: 0.962 ± 0.046
1.47ArgAsn: 1.47 ± 0.053
1.554ArgPro: 1.554 ± 0.059
2.326ArgGln: 2.326 ± 0.079
1.899ArgArg: 1.899 ± 0.074
2.029ArgSer: 2.029 ± 0.065
1.743ArgThr: 1.743 ± 0.059
2.874ArgVal: 2.874 ± 0.088
0.337ArgTrp: 0.337 ± 0.027
1.577ArgTyr: 1.577 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
5.314SerAla: 5.314 ± 0.137
0.164SerCys: 0.164 ± 0.019
3.408SerAsp: 3.408 ± 0.092
2.783SerGlu: 2.783 ± 0.096
2.817SerPhe: 2.817 ± 0.077
4.407SerGly: 4.407 ± 0.119
1.163SerHis: 1.163 ± 0.05
3.438SerIle: 3.438 ± 0.104
3.502SerLys: 3.502 ± 0.086
6.214SerLeu: 6.214 ± 0.113
1.417SerMet: 1.417 ± 0.058
2.633SerAsn: 2.633 ± 0.109
1.867SerPro: 1.867 ± 0.07
3.348SerGln: 3.348 ± 0.125
2.3SerArg: 2.3 ± 0.069
3.816SerSer: 3.816 ± 0.153
3.363SerThr: 3.363 ± 0.101
4.057SerVal: 4.057 ± 0.086
0.702SerTrp: 0.702 ± 0.045
2.066SerTyr: 2.066 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
5.711ThrAla: 5.711 ± 0.138
0.132ThrCys: 0.132 ± 0.017
3.845ThrAsp: 3.845 ± 0.094
2.644ThrGlu: 2.644 ± 0.081
2.52ThrPhe: 2.52 ± 0.079
4.744ThrGly: 4.744 ± 0.119
1.022ThrHis: 1.022 ± 0.046
4.503ThrIle: 4.503 ± 0.09
3.865ThrLys: 3.865 ± 0.105
5.753ThrLeu: 5.753 ± 0.113
1.344ThrMet: 1.344 ± 0.061
2.919ThrAsn: 2.919 ± 0.1
2.499ThrPro: 2.499 ± 0.083
2.076ThrGln: 2.076 ± 0.069
1.831ThrArg: 1.831 ± 0.059
3.333ThrSer: 3.333 ± 0.103
3.954ThrThr: 3.954 ± 0.109
4.955ThrVal: 4.955 ± 0.109
0.691ThrTrp: 0.691 ± 0.042
2.025ThrTyr: 2.025 ± 0.077
0.0ThrXaa: 0.0 ± 0.0
Val
7.341ValAla: 7.341 ± 0.131
0.169ValCys: 0.169 ± 0.02
4.722ValAsp: 4.722 ± 0.114
3.903ValGlu: 3.903 ± 0.105
2.966ValPhe: 2.966 ± 0.083
4.987ValGly: 4.987 ± 0.114
1.214ValHis: 1.214 ± 0.049
5.066ValIle: 5.066 ± 0.105
4.311ValLys: 4.311 ± 0.1
7.509ValLeu: 7.509 ± 0.136
1.812ValMet: 1.812 ± 0.061
3.28ValAsn: 3.28 ± 0.085
2.813ValPro: 2.813 ± 0.076
3.045ValGln: 3.045 ± 0.086
2.527ValArg: 2.527 ± 0.08
4.658ValSer: 4.658 ± 0.107
5.198ValThr: 5.198 ± 0.115
6.27ValVal: 6.27 ± 0.125
0.644ValTrp: 0.644 ± 0.033
2.211ValTyr: 2.211 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.805TrpAla: 0.805 ± 0.047
0.036TrpCys: 0.036 ± 0.009
0.48TrpAsp: 0.48 ± 0.036
0.437TrpGlu: 0.437 ± 0.033
0.578TrpPhe: 0.578 ± 0.033
0.57TrpGly: 0.57 ± 0.038
0.299TrpHis: 0.299 ± 0.026
0.608TrpIle: 0.608 ± 0.034
0.367TrpLys: 0.367 ± 0.029
1.406TrpLeu: 1.406 ± 0.054
0.213TrpMet: 0.213 ± 0.021
0.412TrpAsn: 0.412 ± 0.03
0.359TrpPro: 0.359 ± 0.03
0.749TrpGln: 0.749 ± 0.044
0.508TrpArg: 0.508 ± 0.032
0.681TrpSer: 0.681 ± 0.043
0.57TrpThr: 0.57 ± 0.034
0.687TrpVal: 0.687 ± 0.039
0.166TrpTrp: 0.166 ± 0.02
0.337TrpTyr: 0.337 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.456TyrAla: 2.456 ± 0.072
0.13TyrCys: 0.13 ± 0.02
2.006TyrAsp: 2.006 ± 0.074
1.647TyrGlu: 1.647 ± 0.065
1.692TyrPhe: 1.692 ± 0.063
2.313TyrGly: 2.313 ± 0.074
0.792TyrHis: 0.792 ± 0.041
1.583TyrIle: 1.583 ± 0.056
1.462TyrLys: 1.462 ± 0.056
3.83TyrLeu: 3.83 ± 0.097
0.61TyrMet: 0.61 ± 0.033
1.325TyrAsn: 1.325 ± 0.051
1.366TyrPro: 1.366 ± 0.053
2.599TyrGln: 2.599 ± 0.09
1.613TyrArg: 1.613 ± 0.052
1.756TyrSer: 1.756 ± 0.075
1.65TyrThr: 1.65 ± 0.071
1.993TyrVal: 1.993 ± 0.076
0.431TyrTrp: 0.431 ± 0.031
1.227TyrTyr: 1.227 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1624 proteins (468610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski