Mycobacterium riyadhense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X2BE41|A0A1X2BE41_9MYCO Uncharacterized protein OS=Mycobacterium riyadhense OX=486698 GN=AWC22_04755 PE=4 SV=1
MM1 pKa = 7.52SYY3 pKa = 11.04VLAEE7 pKa = 4.48LDD9 pKa = 3.5MLAAATGDD17 pKa = 3.46MAGIGSSLSAANAAAAASTTGVLAAGGDD45 pKa = 3.92EE46 pKa = 4.31VSAAIAALFSSHH58 pKa = 5.67AQQYY62 pKa = 9.04QVLSAQATEE71 pKa = 4.45FHH73 pKa = 6.32AQFVQALNNAGGAYY87 pKa = 9.85AAAEE91 pKa = 4.19AANGAPLQTLVDD103 pKa = 5.31DD104 pKa = 4.3ILAVINAPTNVLLGRR119 pKa = 11.84PVIGDD124 pKa = 4.06GSNGAAGTGDD134 pKa = 3.19AGGAGGILWGNGGSGGSGAPGQAGGAGGSAGLLGSGGTGGAGGIGGGSGGVGGTGGWLWGNGGAGGAGGVGGLGVNGGAGGVGGSALLFGNGGFGGVGGAGAAGAVGDD242 pKa = 4.54AGTPGTTVFAGGTGGVGGDD261 pKa = 3.82GGTAGNGGAGGNGGLVFGAGGDD283 pKa = 3.74GGQGGVGGAGGTGGAGGAGGEE304 pKa = 4.18PTRR307 pKa = 11.84LGQAGGDD314 pKa = 3.82GGDD317 pKa = 3.9SGTGGNGGDD326 pKa = 3.9GGMGGAGGRR335 pKa = 11.84GSALFGTAGANGNGGAGGAGGDD357 pKa = 3.86AGAPGNGADD366 pKa = 3.82GAAGRR371 pKa = 11.84DD372 pKa = 3.38ASTPGGMGGNGGDD385 pKa = 3.57PGGVGVGGTGGAAGGVGASAGATGTDD411 pKa = 3.53GSIIAGNGGNGGHH424 pKa = 6.78GGAGFNATGSGHH436 pKa = 6.18GGSGGSGGSGGRR448 pKa = 11.84YY449 pKa = 6.5GTGGTGGAGGAAAGDD464 pKa = 4.21GNGGRR469 pKa = 11.84GGDD472 pKa = 3.56GGFGGSMGGAGGDD485 pKa = 3.71GGDD488 pKa = 3.76GGVGAGSGSGGWGGTGGHH506 pKa = 6.49HH507 pKa = 7.2LVTGTANAVGGTGGAGAAGGSTGVGGFGGVGGDD540 pKa = 3.24AKK542 pKa = 10.6IFNDD546 pKa = 3.3ASTAVATGGNGGAGGDD562 pKa = 3.98GASGGNGGDD571 pKa = 3.64GGWAYY576 pKa = 9.99TEE578 pKa = 4.92GIGNVTPGTGGHH590 pKa = 6.7GGTGTTFGGGSGGDD604 pKa = 3.17GGGAQIANGASTVVAVGGTGGAGGDD629 pKa = 4.16GIDD632 pKa = 3.28NSGFTEE638 pKa = 4.49AGHH641 pKa = 6.3GGSGGDD647 pKa = 3.73AFIDD651 pKa = 3.86NTGSTAKK658 pKa = 10.67AIGGTGGAGGSASTGTGGIGGAGGDD683 pKa = 4.2AFNSGAGSAFGGTPGAGGAGPAGGDD708 pKa = 3.48GGQGGAAYY716 pKa = 10.51ALGTGDD722 pKa = 3.53ATGHH726 pKa = 6.54AGAPGTSVGASGVGGAGGGGGDD748 pKa = 4.18ARR750 pKa = 11.84ILNSASTATATAGDD764 pKa = 4.25GGNGGDD770 pKa = 3.89GTSGGSGGYY779 pKa = 9.93GGWAYY784 pKa = 8.6TQGTGNVTAGNGGYY798 pKa = 10.4GGTGSTGGGGSGGEE812 pKa = 4.2GGNVEE817 pKa = 4.75IDD819 pKa = 3.04NDD821 pKa = 4.2AYY823 pKa = 10.93GHH825 pKa = 7.43DD826 pKa = 3.49ITGGNGGAGGTGLNQLDD843 pKa = 3.7GGFGNGGDD851 pKa = 4.16GGTATITSAGSTVNAIGGAGGAGGNATTFAASGGSGGMAINHH893 pKa = 6.24GNGNATGGFAGDD905 pKa = 4.33GGDD908 pKa = 3.9GAHH911 pKa = 6.69GGSGGSGGSAFGYY924 pKa = 10.89GNGLATGGVGGTGGNSSGVGANGGDD949 pKa = 3.71GGDD952 pKa = 3.64GGNAYY957 pKa = 10.44AHH959 pKa = 6.66VYY961 pKa = 8.19PANATEE967 pKa = 4.98GIGGAGGSGDD977 pKa = 3.8PDD979 pKa = 4.63GIAGLDD985 pKa = 4.49GITGPLL991 pKa = 3.32

Molecular weight: 82.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X2AW41|A0A1X2AW41_9MYCO Dipeptidase OS=Mycobacterium riyadhense OX=486698 GN=AWC22_07430 PE=4 SV=1
MM1 pKa = 7.98IFMRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84QRR9 pKa = 11.84TLMFMMALAVIAALLMALFLGYY31 pKa = 10.48HH32 pKa = 6.44FGRR35 pKa = 11.84RR36 pKa = 11.84AGSTPAMRR44 pKa = 11.84KK45 pKa = 7.92RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84AALGGLALSLIVLMVARR67 pKa = 11.84RR68 pKa = 11.84IQQGFVVKK76 pKa = 10.42RR77 pKa = 11.84ALPGAAGGWGLKK89 pKa = 10.26LIEE92 pKa = 4.33ALQLRR97 pKa = 11.84RR98 pKa = 11.84GRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84FHH104 pKa = 7.43RR105 pKa = 11.84PMTRR109 pKa = 11.84WAQVTRR115 pKa = 11.84AA116 pKa = 3.72

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5166

0

5166

1738609

29

5181

336.5

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.082 ± 0.055

0.826 ± 0.011

5.846 ± 0.032

4.83 ± 0.037

3.027 ± 0.026

10.285 ± 0.157

2.186 ± 0.02

4.301 ± 0.026

2.077 ± 0.023

9.648 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.017

2.688 ± 0.055

5.691 ± 0.032

3.117 ± 0.019

7.024 ± 0.047

5.549 ± 0.03

5.977 ± 0.028

8.293 ± 0.04

1.462 ± 0.013

2.102 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski