Pectobacterium phage PP16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Kotilavirus; Pectobacterium virus PP16

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3C9R2|A0A3G3C9R2_9CAUD Uncharacterized protein OS=Pectobacterium phage PP16 OX=1873958 GN=PP16_gp35 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 5.83SNHH5 pKa = 7.22DD6 pKa = 4.1DD7 pKa = 3.65LTVEE11 pKa = 4.37QVSTCYY17 pKa = 10.65DD18 pKa = 2.81IVMDD22 pKa = 5.41SLSCLQRR29 pKa = 11.84AHH31 pKa = 6.82GEE33 pKa = 4.07DD34 pKa = 3.91VACEE38 pKa = 4.36LGQSVGSVIDD48 pKa = 3.3ALFRR52 pKa = 11.84VRR54 pKa = 11.84NAFVDD59 pKa = 3.75KK60 pKa = 11.22

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1PEC7|A0A1B1PEC7_9CAUD Putative 5'-3' exonuclease OS=Pectobacterium phage PP16 OX=1873958 GN=PP16_gp26 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84KK3 pKa = 8.3LTRR6 pKa = 11.84SQVRR10 pKa = 11.84PTAMRR15 pKa = 11.84LLQQQGGVCPLCNKK29 pKa = 9.8PVDD32 pKa = 4.14LTEE35 pKa = 4.52KK36 pKa = 10.37GALVLDD42 pKa = 4.54HH43 pKa = 7.41DD44 pKa = 4.86HH45 pKa = 6.51GTGQVRR51 pKa = 11.84GALHH55 pKa = 6.9RR56 pKa = 11.84SCNSAEE62 pKa = 3.99GKK64 pKa = 9.22VANAAGRR71 pKa = 11.84WGAKK75 pKa = 7.3SMRR78 pKa = 11.84YY79 pKa = 9.14EE80 pKa = 4.59DD81 pKa = 3.22IVPFLEE87 pKa = 4.63RR88 pKa = 11.84LVAYY92 pKa = 9.23LKK94 pKa = 10.34QPPQDD99 pKa = 3.36MIYY102 pKa = 8.45PTFKK106 pKa = 10.29TADD109 pKa = 3.98EE110 pKa = 4.12LRR112 pKa = 11.84MARR115 pKa = 11.84NVKK118 pKa = 9.38EE119 pKa = 3.84RR120 pKa = 11.84TRR122 pKa = 11.84RR123 pKa = 11.84AEE125 pKa = 3.75RR126 pKa = 11.84KK127 pKa = 9.17ARR129 pKa = 11.84EE130 pKa = 3.83TVRR133 pKa = 11.84RR134 pKa = 11.84SRR136 pKa = 11.84VNKK139 pKa = 9.72QDD141 pKa = 3.0

Molecular weight: 16.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

14053

47

1230

246.5

27.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.699 ± 0.453

1.167 ± 0.189

6.383 ± 0.221

5.209 ± 0.327

3.11 ± 0.143

7.372 ± 0.217

2.185 ± 0.182

4.241 ± 0.172

4.22 ± 0.323

8.767 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.925 ± 0.148

4.22 ± 0.262

4.141 ± 0.235

4.547 ± 0.34

6.141 ± 0.266

6.134 ± 0.246

6.589 ± 0.373

7.771 ± 0.238

1.26 ± 0.139

3.921 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski