Amino acid dipepetide frequency for Desulfotomaculum aquiferis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.084AlaAla: 7.084 ± 0.115	0.933AlaCys: 0.933 ± 0.03	3.303AlaAsp: 3.303 ± 0.066	4.899AlaGlu: 4.899 ± 0.069	2.728AlaPhe: 2.728 ± 0.059	6.239AlaGly: 6.239 ± 0.098	1.174AlaHis: 1.174 ± 0.037	5.888AlaIle: 5.888 ± 0.085	4.684AlaLys: 4.684 ± 0.065	7.618AlaLeu: 7.618 ± 0.096	2.41AlaMet: 2.41 ± 0.05	2.93AlaAsn: 2.93 ± 0.053	2.392AlaPro: 2.392 ± 0.051	2.438AlaGln: 2.438 ± 0.049	3.777AlaArg: 3.777 ± 0.064	3.976AlaSer: 3.976 ± 0.063	3.597AlaThr: 3.597 ± 0.064	5.961AlaVal: 5.961 ± 0.087	0.756AlaTrp: 0.756 ± 0.026	2.061AlaTyr: 2.061 ± 0.054	0.005AlaXaa: 0.005 ± 0.002
Cys	0.707CysAla: 0.707 ± 0.028	0.291CysCys: 0.291 ± 0.021	0.463CysAsp: 0.463 ± 0.022	0.563CysGlu: 0.563 ± 0.023	0.492CysPhe: 0.492 ± 0.024	1.168CysGly: 1.168 ± 0.039	0.28CysHis: 0.28 ± 0.019	0.747CysIle: 0.747 ± 0.031	0.617CysLys: 0.617 ± 0.025	1.214CysLeu: 1.214 ± 0.038	0.395CysMet: 0.395 ± 0.019	0.472CysAsn: 0.472 ± 0.023	0.776CysPro: 0.776 ± 0.033	0.549CysGln: 0.549 ± 0.025	0.685CysArg: 0.685 ± 0.024	0.894CysSer: 0.894 ± 0.038	0.615CysThr: 0.615 ± 0.026	0.647CysVal: 0.647 ± 0.03	0.175CysTrp: 0.175 ± 0.015	0.403CysTyr: 0.403 ± 0.022	0.002CysXaa: 0.002 ± 0.001
Asp	2.901AspAla: 2.901 ± 0.057	0.553AspCys: 0.553 ± 0.023	1.919AspAsp: 1.919 ± 0.045	2.992AspGlu: 2.992 ± 0.058	2.195AspPhe: 2.195 ± 0.048	3.119AspGly: 3.119 ± 0.063	0.774AspHis: 0.774 ± 0.024	4.424AspIle: 4.424 ± 0.069	3.156AspLys: 3.156 ± 0.061	4.895AspLeu: 4.895 ± 0.073	1.368AspMet: 1.368 ± 0.035	2.118AspAsn: 2.118 ± 0.049	1.963AspPro: 1.963 ± 0.05	1.554AspGln: 1.554 ± 0.038	2.281AspArg: 2.281 ± 0.045	2.431AspSer: 2.431 ± 0.048	2.162AspThr: 2.162 ± 0.041	3.181AspVal: 3.181 ± 0.06	0.525AspTrp: 0.525 ± 0.023	1.872AspTyr: 1.872 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	5.159GluAla: 5.159 ± 0.081	0.55GluCys: 0.55 ± 0.021	2.975GluAsp: 2.975 ± 0.056	5.372GluGlu: 5.372 ± 0.085	2.288GluPhe: 2.288 ± 0.047	4.31GluGly: 4.31 ± 0.07	1.131GluHis: 1.131 ± 0.038	5.601GluIle: 5.601 ± 0.077	4.999GluLys: 4.999 ± 0.07	7.303GluLeu: 7.303 ± 0.092	2.054GluMet: 2.054 ± 0.048	2.98GluAsn: 2.98 ± 0.058	2.002GluPro: 2.002 ± 0.048	3.211GluGln: 3.211 ± 0.071	3.371GluArg: 3.371 ± 0.066	2.936GluSer: 2.936 ± 0.058	2.993GluThr: 2.993 ± 0.056	5.076GluVal: 5.076 ± 0.08	0.504GluTrp: 0.504 ± 0.023	2.03GluTyr: 2.03 ± 0.046	0.005GluXaa: 0.005 ± 0.002
Phe	2.785PheAla: 2.785 ± 0.053	0.533PheCys: 0.533 ± 0.024	1.909PheAsp: 1.909 ± 0.044	2.256PheGlu: 2.256 ± 0.05	1.71PhePhe: 1.71 ± 0.046	2.949PheGly: 2.949 ± 0.059	0.724PheHis: 0.724 ± 0.029	3.202PheIle: 3.202 ± 0.059	2.241PheLys: 2.241 ± 0.056	4.145PheLeu: 4.145 ± 0.076	1.217PheMet: 1.217 ± 0.037	1.905PheAsn: 1.905 ± 0.051	1.651PhePro: 1.651 ± 0.041	1.263PheGln: 1.263 ± 0.039	1.676PheArg: 1.676 ± 0.038	2.786PheSer: 2.786 ± 0.064	2.109PheThr: 2.109 ± 0.047	2.542PheVal: 2.542 ± 0.054	0.453PheTrp: 0.453 ± 0.024	1.396PheTyr: 1.396 ± 0.045	0.004PheXaa: 0.004 ± 0.002
Gly	5.323GlyAla: 5.323 ± 0.085	1.184GlyCys: 1.184 ± 0.034	3.262GlyAsp: 3.262 ± 0.063	4.539GlyGlu: 4.539 ± 0.082	3.14GlyPhe: 3.14 ± 0.062	5.3GlyGly: 5.3 ± 0.083	1.44GlyHis: 1.44 ± 0.04	6.194GlyIle: 6.194 ± 0.082	5.018GlyLys: 5.018 ± 0.076	7.8GlyLeu: 7.8 ± 0.092	2.385GlyMet: 2.385 ± 0.05	2.929GlyAsn: 2.929 ± 0.053	2.199GlyPro: 2.199 ± 0.049	2.985GlyGln: 2.985 ± 0.066	3.447GlyArg: 3.447 ± 0.061	4.12GlySer: 4.12 ± 0.069	3.99GlyThr: 3.99 ± 0.067	5.59GlyVal: 5.59 ± 0.079	0.8GlyTrp: 0.8 ± 0.028	2.801GlyTyr: 2.801 ± 0.061	0.012GlyXaa: 0.012 ± 0.003
His	1.031HisAla: 1.031 ± 0.038	0.317HisCys: 0.317 ± 0.019	0.744HisAsp: 0.744 ± 0.028	0.922HisGlu: 0.922 ± 0.033	0.735HisPhe: 0.735 ± 0.026	1.298HisGly: 1.298 ± 0.038	0.459HisHis: 0.459 ± 0.024	1.332HisIle: 1.332 ± 0.039	0.914HisLys: 0.914 ± 0.032	1.824HisLeu: 1.824 ± 0.049	0.526HisMet: 0.526 ± 0.022	0.721HisAsn: 0.721 ± 0.03	0.973HisPro: 0.973 ± 0.031	0.758HisGln: 0.758 ± 0.03	1.054HisArg: 1.054 ± 0.035	1.045HisSer: 1.045 ± 0.032	0.851HisThr: 0.851 ± 0.031	0.973HisVal: 0.973 ± 0.03	0.25HisTrp: 0.25 ± 0.016	0.61HisTyr: 0.61 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.69IleAla: 5.69 ± 0.079	0.949IleCys: 0.949 ± 0.03	3.775IleAsp: 3.775 ± 0.061	4.797IleGlu: 4.797 ± 0.068	3.218IlePhe: 3.218 ± 0.068	5.442IleGly: 5.442 ± 0.084	1.19IleHis: 1.19 ± 0.041	6.454IleIle: 6.454 ± 0.087	5.389IleLys: 5.389 ± 0.086	7.668IleLeu: 7.668 ± 0.084	2.163IleMet: 2.163 ± 0.051	4.074IleAsn: 4.074 ± 0.062	3.498IlePro: 3.498 ± 0.065	2.343IleGln: 2.343 ± 0.05	3.417IleArg: 3.417 ± 0.063	5.335IleSer: 5.335 ± 0.072	4.431IleThr: 4.431 ± 0.068	4.964IleVal: 4.964 ± 0.072	0.687IleTrp: 0.687 ± 0.028	2.358IleTyr: 2.358 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.543LysAla: 4.543 ± 0.067	0.608LysCys: 0.608 ± 0.027	3.46LysAsp: 3.46 ± 0.055	5.326LysGlu: 5.326 ± 0.079	1.884LysPhe: 1.884 ± 0.044	4.383LysGly: 4.383 ± 0.059	1.052LysHis: 1.052 ± 0.032	4.813LysIle: 4.813 ± 0.074	4.16LysLys: 4.16 ± 0.068	6.269LysLeu: 6.269 ± 0.078	1.923LysMet: 1.923 ± 0.044	2.944LysAsn: 2.944 ± 0.06	2.494LysPro: 2.494 ± 0.051	2.296LysGln: 2.296 ± 0.052	2.909LysArg: 2.909 ± 0.062	3.241LysSer: 3.241 ± 0.052	3.143LysThr: 3.143 ± 0.062	4.736LysVal: 4.736 ± 0.07	0.578LysTrp: 0.578 ± 0.024	2.263LysTyr: 2.263 ± 0.057	0.009LysXaa: 0.009 ± 0.003
Leu	9.166LeuAla: 9.166 ± 0.112	1.123LeuCys: 1.123 ± 0.038	4.866LeuAsp: 4.866 ± 0.081	6.967LeuGlu: 6.967 ± 0.084	3.991LeuPhe: 3.991 ± 0.083	7.788LeuGly: 7.788 ± 0.097	1.578LeuHis: 1.578 ± 0.045	7.206LeuIle: 7.206 ± 0.099	6.659LeuLys: 6.659 ± 0.082	10.471LeuLeu: 10.471 ± 0.134	3.015LeuMet: 3.015 ± 0.055	4.455LeuAsn: 4.455 ± 0.071	4.808LeuPro: 4.808 ± 0.073	3.402LeuGln: 3.402 ± 0.059	4.692LeuArg: 4.692 ± 0.069	6.622LeuSer: 6.622 ± 0.086	5.561LeuThr: 5.561 ± 0.07	7.583LeuVal: 7.583 ± 0.092	1.054LeuTrp: 1.054 ± 0.033	2.907LeuTyr: 2.907 ± 0.05	0.007LeuXaa: 0.007 ± 0.003
Met	2.817MetAla: 2.817 ± 0.05	0.259MetCys: 0.259 ± 0.016	1.542MetAsp: 1.542 ± 0.046	2.166MetGlu: 2.166 ± 0.047	1.001MetPhe: 1.001 ± 0.031	2.381MetGly: 2.381 ± 0.048	0.442MetHis: 0.442 ± 0.02	1.952MetIle: 1.952 ± 0.047	1.783MetLys: 1.783 ± 0.043	3.15MetLeu: 3.15 ± 0.064	0.772MetMet: 0.772 ± 0.032	1.171MetAsn: 1.171 ± 0.036	1.344MetPro: 1.344 ± 0.037	1.01MetGln: 1.01 ± 0.031	1.245MetArg: 1.245 ± 0.037	1.771MetSer: 1.771 ± 0.039	1.573MetThr: 1.573 ± 0.04	2.51MetVal: 2.51 ± 0.053	0.202MetTrp: 0.202 ± 0.014	0.667MetTyr: 0.667 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.486AsnAla: 2.486 ± 0.05	0.657AsnCys: 0.657 ± 0.028	1.728AsnAsp: 1.728 ± 0.046	2.507AsnGlu: 2.507 ± 0.052	1.783AsnPhe: 1.783 ± 0.041	2.712AsnGly: 2.712 ± 0.049	0.869AsnHis: 0.869 ± 0.031	3.985AsnIle: 3.985 ± 0.07	3.016AsnLys: 3.016 ± 0.05	4.795AsnLeu: 4.795 ± 0.082	1.306AsnMet: 1.306 ± 0.036	2.369AsnAsn: 2.369 ± 0.059	2.334AsnPro: 2.334 ± 0.047	1.832AsnGln: 1.832 ± 0.045	2.192AsnArg: 2.192 ± 0.049	2.542AsnSer: 2.542 ± 0.05	2.217AsnThr: 2.217 ± 0.053	2.617AsnVal: 2.617 ± 0.05	0.584AsnTrp: 0.584 ± 0.024	1.671AsnTyr: 1.671 ± 0.045	0.002AsnXaa: 0.002 ± 0.001
Pro	2.95ProAla: 2.95 ± 0.065	0.465ProCys: 0.465 ± 0.023	2.094ProAsp: 2.094 ± 0.052	3.408ProGlu: 3.408 ± 0.062	1.666ProPhe: 1.666 ± 0.044	3.709ProGly: 3.709 ± 0.059	0.738ProHis: 0.738 ± 0.028	2.68ProIle: 2.68 ± 0.051	2.218ProLys: 2.218 ± 0.047	4.04ProLeu: 4.04 ± 0.07	1.291ProMet: 1.291 ± 0.035	1.588ProAsn: 1.588 ± 0.043	1.548ProPro: 1.548 ± 0.046	1.387ProGln: 1.387 ± 0.036	1.795ProArg: 1.795 ± 0.045	2.135ProSer: 2.135 ± 0.052	2.028ProThr: 2.028 ± 0.047	3.538ProVal: 3.538 ± 0.066	0.607ProTrp: 0.607 ± 0.025	1.327ProTyr: 1.327 ± 0.038	0.003ProXaa: 0.003 ± 0.002
Gln	2.935GlnAla: 2.935 ± 0.058	0.371GlnCys: 0.371 ± 0.02	1.702GlnAsp: 1.702 ± 0.046	2.924GlnGlu: 2.924 ± 0.057	1.135GlnPhe: 1.135 ± 0.037	2.822GlnGly: 2.822 ± 0.053	0.63GlnHis: 0.63 ± 0.023	2.751GlnIle: 2.751 ± 0.055	2.157GlnLys: 2.157 ± 0.046	3.611GlnLeu: 3.611 ± 0.067	1.111GlnMet: 1.111 ± 0.035	1.531GlnAsn: 1.531 ± 0.039	1.354GlnPro: 1.354 ± 0.04	1.759GlnGln: 1.759 ± 0.052	2.09GlnArg: 2.09 ± 0.054	1.87GlnSer: 1.87 ± 0.049	1.675GlnThr: 1.675 ± 0.045	2.955GlnVal: 2.955 ± 0.055	0.411GlnTrp: 0.411 ± 0.024	1.13GlnTyr: 1.13 ± 0.033	0.003GlnXaa: 0.003 ± 0.002
Arg	3.122ArgAla: 3.122 ± 0.063	0.597ArgCys: 0.597 ± 0.025	2.333ArgAsp: 2.333 ± 0.044	3.746ArgGlu: 3.746 ± 0.074	1.899ArgPhe: 1.899 ± 0.038	3.105ArgGly: 3.105 ± 0.06	0.89ArgHis: 0.89 ± 0.031	3.468ArgIle: 3.468 ± 0.063	2.91ArgLys: 2.91 ± 0.061	5.064ArgLeu: 5.064 ± 0.071	1.486ArgMet: 1.486 ± 0.035	2.089ArgAsn: 2.089 ± 0.054	1.955ArgPro: 1.955 ± 0.051	2.153ArgGln: 2.153 ± 0.047	2.506ArgArg: 2.506 ± 0.055	2.383ArgSer: 2.383 ± 0.05	2.145ArgThr: 2.145 ± 0.042	3.476ArgVal: 3.476 ± 0.058	0.613ArgTrp: 0.613 ± 0.028	1.658ArgTyr: 1.658 ± 0.049	0.004ArgXaa: 0.004 ± 0.002
Ser	3.706SerAla: 3.706 ± 0.068	0.759SerCys: 0.759 ± 0.03	2.438SerAsp: 2.438 ± 0.051	3.372SerGlu: 3.372 ± 0.061	2.657SerPhe: 2.657 ± 0.051	4.669SerGly: 4.669 ± 0.07	1.086SerHis: 1.086 ± 0.035	4.469SerIle: 4.469 ± 0.069	3.432SerLys: 3.432 ± 0.059	6.411SerLeu: 6.411 ± 0.081	1.694SerMet: 1.694 ± 0.044	2.453SerAsn: 2.453 ± 0.054	2.457SerPro: 2.457 ± 0.053	2.136SerGln: 2.136 ± 0.05	2.831SerArg: 2.831 ± 0.054	3.709SerSer: 3.709 ± 0.069	2.956SerThr: 2.956 ± 0.056	3.948SerVal: 3.948 ± 0.064	0.683SerTrp: 0.683 ± 0.029	1.866SerTyr: 1.866 ± 0.047	0.006SerXaa: 0.006 ± 0.002
Thr	3.959ThrAla: 3.959 ± 0.069	0.578ThrCys: 0.578 ± 0.027	2.303ThrAsp: 2.303 ± 0.052	2.916ThrGlu: 2.916 ± 0.058	2.058ThrPhe: 2.058 ± 0.05	4.772ThrGly: 4.772 ± 0.077	0.885ThrHis: 0.885 ± 0.033	3.987ThrIle: 3.987 ± 0.064	2.799ThrLys: 2.799 ± 0.059	5.065ThrLeu: 5.065 ± 0.076	1.384ThrMet: 1.384 ± 0.03	2.224ThrAsn: 2.224 ± 0.049	2.458ThrPro: 2.458 ± 0.05	1.429ThrGln: 1.429 ± 0.04	2.199ThrArg: 2.199 ± 0.047	2.978ThrSer: 2.978 ± 0.063	2.804ThrThr: 2.804 ± 0.066	3.965ThrVal: 3.965 ± 0.067	0.535ThrTrp: 0.535 ± 0.025	1.524ThrTyr: 1.524 ± 0.046	0.002ThrXaa: 0.002 ± 0.001
Val	5.925ValAla: 5.925 ± 0.101	0.817ValCys: 0.817 ± 0.03	3.583ValAsp: 3.583 ± 0.07	4.831ValGlu: 4.831 ± 0.077	2.922ValPhe: 2.922 ± 0.069	5.211ValGly: 5.211 ± 0.074	1.099ValHis: 1.099 ± 0.035	5.771ValIle: 5.771 ± 0.087	4.459ValLys: 4.459 ± 0.078	7.43ValLeu: 7.43 ± 0.087	2.053ValMet: 2.053 ± 0.047	3.286ValAsn: 3.286 ± 0.058	2.973ValPro: 2.973 ± 0.053	2.331ValGln: 2.331 ± 0.051	3.156ValArg: 3.156 ± 0.051	4.351ValSer: 4.351 ± 0.064	4.0ValThr: 4.0 ± 0.064	5.752ValVal: 5.752 ± 0.094	0.601ValTrp: 0.601 ± 0.029	2.003ValTyr: 2.003 ± 0.044	0.004ValXaa: 0.004 ± 0.002
Trp	0.655TrpAla: 0.655 ± 0.023	0.138TrpCys: 0.138 ± 0.011	0.48TrpAsp: 0.48 ± 0.021	0.671TrpGlu: 0.671 ± 0.029	0.44TrpPhe: 0.44 ± 0.024	0.722TrpGly: 0.722 ± 0.028	0.202TrpHis: 0.202 ± 0.013	0.615TrpIle: 0.615 ± 0.024	0.578TrpLys: 0.578 ± 0.023	1.309TrpLeu: 1.309 ± 0.039	0.362TrpMet: 0.362 ± 0.018	0.415TrpAsn: 0.415 ± 0.019	0.546TrpPro: 0.546 ± 0.025	0.634TrpGln: 0.634 ± 0.024	0.529TrpArg: 0.529 ± 0.023	0.646TrpSer: 0.646 ± 0.028	0.404TrpThr: 0.404 ± 0.021	0.712TrpVal: 0.712 ± 0.03	0.227TrpTrp: 0.227 ± 0.016	0.331TrpTyr: 0.331 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.906TyrAla: 1.906 ± 0.048	0.472TyrCys: 0.472 ± 0.024	1.504TyrAsp: 1.504 ± 0.045	1.674TyrGlu: 1.674 ± 0.042	1.51TyrPhe: 1.51 ± 0.04	2.35TyrGly: 2.35 ± 0.052	0.692TyrHis: 0.692 ± 0.026	2.304TyrIle: 2.304 ± 0.053	1.838TyrLys: 1.838 ± 0.046	3.83TyrLeu: 3.83 ± 0.061	0.84TyrMet: 0.84 ± 0.032	1.55TyrAsn: 1.55 ± 0.042	1.488TyrPro: 1.488 ± 0.039	1.452TyrGln: 1.452 ± 0.038	1.746TyrArg: 1.746 ± 0.041	1.963TyrSer: 1.963 ± 0.045	1.567TyrThr: 1.567 ± 0.042	1.823TyrVal: 1.823 ± 0.041	0.367TyrTrp: 0.367 ± 0.02	1.296TyrTyr: 1.296 ± 0.037	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.006XaaGlu: 0.006 ± 0.003	0.004XaaPhe: 0.004 ± 0.002	0.007XaaGly: 0.007 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.006XaaLys: 0.006 ± 0.003	0.008XaaLeu: 0.008 ± 0.003	0.003XaaMet: 0.003 ± 0.002	0.002XaaAsn: 0.002 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.004XaaGln: 0.004 ± 0.002	0.002XaaArg: 0.002 ± 0.001	0.003XaaSer: 0.003 ± 0.002	0.004XaaThr: 0.004 ± 0.002	0.006XaaVal: 0.006 ± 0.003	0.003XaaTrp: 0.003 ± 0.002	0.002XaaTyr: 0.002 ± 0.002	0.007XaaXaa: 0.007 ± 0.004

Statistics based on 6019 proteins (955997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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