Mycobacterium phage IdentityCrisis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TH17|A0A5J6TH17_9CAUD Scaffolding protein OS=Mycobacterium phage IdentityCrisis OX=2599866 GN=6 PE=4 SV=1
MM1 pKa = 7.89AYY3 pKa = 10.34AEE5 pKa = 4.99LLDD8 pKa = 3.81TDD10 pKa = 3.83MSTWCPITLHH20 pKa = 6.07YY21 pKa = 10.5KK22 pKa = 8.71VTDD25 pKa = 3.9GDD27 pKa = 3.94QVSYY31 pKa = 10.88LAVTRR36 pKa = 11.84VSFVTAAGRR45 pKa = 11.84VEE47 pKa = 5.67AFACDD52 pKa = 3.6EE53 pKa = 4.39TGLATSLAPLWAIDD67 pKa = 3.72GDD69 pKa = 4.15VGHH72 pKa = 6.94NDD74 pKa = 3.21ALVAAGYY81 pKa = 7.32TVSS84 pKa = 3.59

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6THE4|A0A5J6THE4_9CAUD Helix-turn-helix DNA binding domain protein OS=Mycobacterium phage IdentityCrisis OX=2599866 GN=57 PE=4 SV=1
MM1 pKa = 7.53SAQTSRR7 pKa = 11.84GNGIGFGTVLFIVFLVLKK25 pKa = 8.85LTGVVTWSWWWVFSPLWIPGVVIAVCLVLAGVLKK59 pKa = 10.73ARR61 pKa = 11.84PP62 pKa = 3.47

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12261

36

913

204.3

21.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.454 ± 0.644

1.044 ± 0.144

6.59 ± 0.249

5.252 ± 0.296

2.528 ± 0.176

9.412 ± 0.746

1.974 ± 0.191

5.016 ± 0.275

3.107 ± 0.278

8.384 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.933 ± 0.101

2.912 ± 0.175

5.652 ± 0.245

3.368 ± 0.131

6.884 ± 0.476

5.399 ± 0.245

6.81 ± 0.334

7.185 ± 0.284

2.039 ± 0.123

2.055 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski