CRESS virus sp. ctin15
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WB13|A0A5Q2WB13_9VIRU Rep protein OS=CRESS virus sp. ctin15 OX=2656686 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 6.09 CYY4 pKa = 11.41 SCFYY8 pKa = 9.02 TEE10 pKa = 5.16 KK11 pKa = 10.13 YY12 pKa = 10.21 SSSSSDD18 pKa = 3.14 SSSDD22 pKa = 3.65 DD23 pKa = 4.01 SKK25 pKa = 11.82 DD26 pKa = 3.29 KK27 pKa = 10.97 KK28 pKa = 10.48 KK29 pKa = 10.82 KK30 pKa = 10.3 KK31 pKa = 10.29 EE32 pKa = 3.94 EE33 pKa = 4.01 LDD35 pKa = 3.85 DD36 pKa = 4.31 YY37 pKa = 11.59 INFIDD42 pKa = 3.99 NPFDD46 pKa = 3.31 ISQIDD51 pKa = 3.43 IPLSNNVSYY60 pKa = 11.44 LDD62 pKa = 3.79 YY63 pKa = 10.09 PYY65 pKa = 11.22 LMPVSSSVSPGYY77 pKa = 9.32 TIINRR82 pKa = 11.84 DD83 pKa = 3.22 IQTLNEE89 pKa = 3.81 MRR91 pKa = 11.84 PIPFNLNSPFTEE103 pKa = 4.26 EE104 pKa = 4.38 GLTTALSNYY113 pKa = 9.01 FGTGSYY119 pKa = 10.53 PIFTKK124 pKa = 10.64 ASPSAQYY131 pKa = 10.61 LVQEE135 pKa = 4.5 GMPIYY140 pKa = 9.92 TGPFDD145 pKa = 5.28 KK146 pKa = 10.8 NGPMNPDD153 pKa = 3.18 DD154 pKa = 3.83 EE155 pKa = 5.49 ARR157 pKa = 11.84 FLKK160 pKa = 10.82 DD161 pKa = 3.93 EE162 pKa = 4.47 LEE164 pKa = 4.0 PWIRR168 pKa = 11.84 LHH170 pKa = 5.97 PHH172 pKa = 6.37 VIPIIGEE179 pKa = 4.14 CVMEE183 pKa = 4.26 NPGFHH188 pKa = 7.24 PSYY191 pKa = 9.49 KK192 pKa = 9.88 QRR194 pKa = 11.84 GNRR197 pKa = 11.84 YY198 pKa = 9.77 SFIDD202 pKa = 3.19 INGKK206 pKa = 7.58 QRR208 pKa = 11.84 YY209 pKa = 6.55 TYY211 pKa = 10.59 GFKK214 pKa = 9.46 PTWDD218 pKa = 3.62 MFLKK222 pKa = 9.86 PDD224 pKa = 4.12 KK225 pKa = 10.75 YY226 pKa = 10.96 PMVVEE231 pKa = 4.15 MGNLRR236 pKa = 11.84 TSTRR240 pKa = 11.84 PVRR243 pKa = 11.84 EE244 pKa = 4.02 PFRR247 pKa = 11.84 GFSPPMYY254 pKa = 10.64 ASDD257 pKa = 4.02 EE258 pKa = 4.17 EE259 pKa = 4.21 MDD261 pKa = 4.2 GYY263 pKa = 11.01 EE264 pKa = 4.8 LNPSTLPSVVPPPPPPPPPPPPPTPTPVDD293 pKa = 3.76 PNIISPIYY301 pKa = 10.0 YY302 pKa = 10.04 SYY304 pKa = 11.28 PLLFWNEE311 pKa = 3.79 PNIISGSSYY320 pKa = 10.41 EE321 pKa = 4.04 IPQLNSAGLCLNSDD335 pKa = 3.92 CSIGGVRR342 pKa = 11.84 SIEE345 pKa = 3.98 APNIDD350 pKa = 3.68 RR351 pKa = 11.84 FIIKK355 pKa = 10.07 IPDD358 pKa = 3.45 NAISRR363 pKa = 11.84 VLNVKK368 pKa = 10.0 KK369 pKa = 10.79 VFYY372 pKa = 9.1 KK373 pKa = 10.85 TNLNQNLPIIIKK385 pKa = 8.84 LKK387 pKa = 9.2 CTVLPVVNNYY397 pKa = 9.71 PLILTSAPDD406 pKa = 4.36 AFPMLYY412 pKa = 9.27 TLTIPTVNYY421 pKa = 9.11 PLGFTGISILFNTGSSSPSTGGEE444 pKa = 3.79 IIFDD448 pKa = 3.72 EE449 pKa = 4.71 VQTYY453 pKa = 8.34 WDD455 pKa = 3.94 PRR457 pKa = 11.84 VKK459 pKa = 10.86 LNLTTHH465 pKa = 6.84 IAMNSLFNGGTPNVQADD482 pKa = 3.83 NGSATLSGVSKK493 pKa = 9.76 TIEE496 pKa = 3.87 YY497 pKa = 10.8 RR498 pKa = 11.84 NGTNNTNLRR507 pKa = 11.84 FQRR510 pKa = 11.84 NIGLNWNTYY519 pKa = 6.71 VQYY522 pKa = 11.3 LNRR525 pKa = 11.84 TRR527 pKa = 11.84 SEE529 pKa = 3.95 FNYY532 pKa = 10.42 AGYY535 pKa = 11.37 AMQGEE540 pKa = 4.7 SQYY543 pKa = 11.6 SRR545 pKa = 11.84 GFTGSANIIGTLGTLGTKK563 pKa = 10.53 LNFVAAGVATAAPTTTVTRR582 pKa = 11.84 RR583 pKa = 11.84 VDD585 pKa = 3.11 IFSNDD590 pKa = 3.39 YY591 pKa = 11.25 LNSQFVNYY599 pKa = 10.25 GYY601 pKa = 10.87 LHH603 pKa = 6.29 YY604 pKa = 10.59 IITT607 pKa = 4.05
Molecular weight: 67.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.976
IPC2_protein 5.219
IPC_protein 5.169
Toseland 5.092
ProMoST 5.308
Dawson 5.156
Bjellqvist 5.283
Wikipedia 5.054
Rodwell 5.067
Grimsley 5.016
Solomon 5.156
Lehninger 5.118
Nozaki 5.283
DTASelect 5.461
Thurlkill 5.105
EMBOSS 5.092
Sillero 5.347
Patrickios 3.083
IPC_peptide 5.169
IPC2_peptide 5.347
IPC2.peptide.svr19 5.306
Protein with the highest isoelectric point:
>tr|A0A5Q2W8K2|A0A5Q2W8K2_9VIRU Actin OS=CRESS virus sp. ctin15 OX=2656686 PE=4 SV=1
MM1 pKa = 7.52 TKK3 pKa = 10.38 LSHH6 pKa = 6.83 KK7 pKa = 10.5 FSLCKK12 pKa = 9.85 MISDD16 pKa = 4.5 LTTIKK21 pKa = 11.05 NPFNTAAFASKK32 pKa = 10.86 VPDD35 pKa = 4.02 GKK37 pKa = 10.33 CGYY40 pKa = 9.95 SIGVKK45 pKa = 9.97 RR46 pKa = 11.84 QVSKK50 pKa = 11.03 QIVSSKK56 pKa = 8.86 GAKK59 pKa = 9.57 IFVLTPTFNCSGFNCDD75 pKa = 3.04 VVGNVIDD82 pKa = 4.7 GIEE85 pKa = 3.91 YY86 pKa = 8.52 HH87 pKa = 6.33 TFSDD91 pKa = 3.31 SDD93 pKa = 3.41 ITVRR97 pKa = 11.84 APSATALAGTAAATVEE113 pKa = 4.42 TNLTLGNNSPEE124 pKa = 4.05 KK125 pKa = 9.89 WRR127 pKa = 11.84 MVSAGMKK134 pKa = 8.56 ITLTNNSEE142 pKa = 4.35 KK143 pKa = 10.67 NDD145 pKa = 3.04 GWFEE149 pKa = 4.64 AIRR152 pKa = 11.84 VNASPSSKK160 pKa = 10.79 DD161 pKa = 3.39 YY162 pKa = 11.63 VFLKK166 pKa = 10.86 NVGQDD171 pKa = 3.21 EE172 pKa = 5.46 GILGVHH178 pKa = 6.89 PFFEE182 pKa = 5.03 DD183 pKa = 3.65 SIIGKK188 pKa = 8.97 SEE190 pKa = 3.47 SWVNHH195 pKa = 5.48 PSYY198 pKa = 10.52 VTGKK202 pKa = 10.25 LRR204 pKa = 11.84 DD205 pKa = 3.56 LHH207 pKa = 7.95 KK208 pKa = 10.7 HH209 pKa = 4.78 MFILQQVNGSRR220 pKa = 11.84 DD221 pKa = 3.26 FCTIRR226 pKa = 11.84 QNNTSSGFKK235 pKa = 10.4 DD236 pKa = 3.3 PQIPIGAFKK245 pKa = 10.62 SGPSEE250 pKa = 3.99 AFTFKK255 pKa = 10.75 NADD258 pKa = 4.24 EE259 pKa = 4.43 IEE261 pKa = 4.04 HH262 pKa = 6.47 WADD265 pKa = 3.62 DD266 pKa = 3.82 SCFDD270 pKa = 3.71 VVLVKK275 pKa = 10.55 CYY277 pKa = 10.64 SATDD281 pKa = 3.73 TVGSSTTNLHH291 pKa = 5.37 LHH293 pKa = 5.66 MVHH296 pKa = 5.2 NHH298 pKa = 4.83 EE299 pKa = 4.88 YY300 pKa = 9.99 IYY302 pKa = 11.0 DD303 pKa = 3.86 SLSTYY308 pKa = 11.22 SKK310 pKa = 10.64 FQTPTSNMASSVLRR324 pKa = 11.84 ATALINSDD332 pKa = 4.29 SKK334 pKa = 11.24 PSIPFDD340 pKa = 3.63 SAPGQLSTCIQACKK354 pKa = 9.14 KK355 pKa = 8.92 TSNKK359 pKa = 9.89 SGRR362 pKa = 11.84 SKK364 pKa = 7.6 TTRR367 pKa = 11.84 SRR369 pKa = 11.84 KK370 pKa = 9.13 KK371 pKa = 10.83 SKK373 pKa = 9.87 ICTTKK378 pKa = 9.53 RR379 pKa = 11.84 RR380 pKa = 11.84 SYY382 pKa = 10.98 KK383 pKa = 9.58 RR384 pKa = 11.84 TGCACKK390 pKa = 10.52 SPTRR394 pKa = 11.84 PPSKK398 pKa = 10.25 RR399 pKa = 11.84 IRR401 pKa = 11.84 KK402 pKa = 9.08 VSAA405 pKa = 3.23
Molecular weight: 44.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.921
IPC2_protein 8.712
IPC_protein 8.565
Toseland 9.472
ProMoST 9.165
Dawson 9.692
Bjellqvist 9.458
Wikipedia 9.809
Rodwell 10.145
Grimsley 9.721
Solomon 9.721
Lehninger 9.706
Nozaki 9.721
DTASelect 9.37
Thurlkill 9.589
EMBOSS 9.897
Sillero 9.692
Patrickios 5.715
IPC_peptide 9.721
IPC2_peptide 8.17
IPC2.peptide.svr19 7.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1466
136
607
366.5
41.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.775 ± 0.657
1.705 ± 0.419
5.389 ± 0.238
5.116 ± 0.852
4.98 ± 0.665
5.73 ± 0.506
2.115 ± 0.59
6.617 ± 0.54
6.617 ± 1.228
6.821 ± 0.576
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.319 ± 0.215
6.617 ± 0.743
6.48 ± 1.594
2.865 ± 0.357
4.638 ± 0.434
8.868 ± 1.099
7.231 ± 0.849
5.935 ± 0.524
0.887 ± 0.039
4.297 ± 1.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here