Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S28-C3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.779AlaAla: 10.779 ± 1.053
0.698AlaCys: 0.698 ± 0.249
6.591AlaAsp: 6.591 ± 1.146
6.591AlaGlu: 6.591 ± 0.768
3.567AlaPhe: 3.567 ± 0.544
4.885AlaGly: 4.885 ± 0.677
1.008AlaHis: 1.008 ± 0.259
4.498AlaIle: 4.498 ± 0.64
5.738AlaLys: 5.738 ± 0.765
7.289AlaLeu: 7.289 ± 0.702
3.179AlaMet: 3.179 ± 0.434
3.955AlaAsn: 3.955 ± 0.863
3.024AlaPro: 3.024 ± 0.498
4.885AlaGln: 4.885 ± 0.994
4.032AlaArg: 4.032 ± 0.572
4.885AlaSer: 4.885 ± 0.674
6.048AlaThr: 6.048 ± 1.037
4.885AlaVal: 4.885 ± 0.669
0.775AlaTrp: 0.775 ± 0.255
2.481AlaTyr: 2.481 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.698CysAla: 0.698 ± 0.225
0.078CysCys: 0.078 ± 0.077
0.31CysAsp: 0.31 ± 0.168
0.465CysGlu: 0.465 ± 0.18
0.155CysPhe: 0.155 ± 0.11
0.775CysGly: 0.775 ± 0.257
0.31CysHis: 0.31 ± 0.179
0.388CysIle: 0.388 ± 0.201
0.698CysLys: 0.698 ± 0.217
0.931CysLeu: 0.931 ± 0.352
0.543CysMet: 0.543 ± 0.27
0.388CysAsn: 0.388 ± 0.198
0.155CysPro: 0.155 ± 0.102
0.0CysGln: 0.0 ± 0.0
0.543CysArg: 0.543 ± 0.247
0.698CysSer: 0.698 ± 0.263
0.853CysThr: 0.853 ± 0.351
0.775CysVal: 0.775 ± 0.262
0.155CysTrp: 0.155 ± 0.108
0.233CysTyr: 0.233 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
6.514AspAla: 6.514 ± 1.09
0.698AspCys: 0.698 ± 0.264
4.498AspAsp: 4.498 ± 0.823
4.498AspGlu: 4.498 ± 0.784
2.016AspPhe: 2.016 ± 0.353
5.04AspGly: 5.04 ± 0.892
1.241AspHis: 1.241 ± 0.443
4.808AspIle: 4.808 ± 0.667
2.481AspLys: 2.481 ± 0.481
6.048AspLeu: 6.048 ± 0.625
1.551AspMet: 1.551 ± 0.353
2.947AspAsn: 2.947 ± 0.572
2.947AspPro: 2.947 ± 0.526
1.706AspGln: 1.706 ± 0.447
3.257AspArg: 3.257 ± 0.547
4.187AspSer: 4.187 ± 0.596
3.489AspThr: 3.489 ± 0.632
4.808AspVal: 4.808 ± 0.624
1.241AspTrp: 1.241 ± 0.316
2.404AspTyr: 2.404 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
5.971GluAla: 5.971 ± 0.658
0.465GluCys: 0.465 ± 0.206
2.792GluAsp: 2.792 ± 0.487
4.11GluGlu: 4.11 ± 0.717
2.326GluPhe: 2.326 ± 0.516
4.498GluGly: 4.498 ± 0.465
0.853GluHis: 0.853 ± 0.196
3.334GluIle: 3.334 ± 0.543
3.489GluLys: 3.489 ± 0.556
6.048GluLeu: 6.048 ± 0.978
1.861GluMet: 1.861 ± 0.415
1.861GluAsn: 1.861 ± 0.392
2.249GluPro: 2.249 ± 0.461
3.877GluGln: 3.877 ± 0.796
4.42GluArg: 4.42 ± 0.821
3.024GluSer: 3.024 ± 0.46
3.489GluThr: 3.489 ± 0.664
4.032GluVal: 4.032 ± 0.609
1.008GluTrp: 1.008 ± 0.27
1.473GluTyr: 1.473 ± 0.331
0.0GluXaa: 0.0 ± 0.0
Phe
2.249PheAla: 2.249 ± 0.469
0.543PheCys: 0.543 ± 0.298
2.326PheAsp: 2.326 ± 0.422
1.551PheGlu: 1.551 ± 0.44
1.939PhePhe: 1.939 ± 0.373
1.939PheGly: 1.939 ± 0.368
0.233PheHis: 0.233 ± 0.136
2.792PheIle: 2.792 ± 0.378
1.628PheLys: 1.628 ± 0.292
2.559PheLeu: 2.559 ± 0.703
0.853PheMet: 0.853 ± 0.198
1.551PheAsn: 1.551 ± 0.276
0.853PhePro: 0.853 ± 0.299
1.861PheGln: 1.861 ± 0.456
1.939PheArg: 1.939 ± 0.307
3.024PheSer: 3.024 ± 0.478
3.877PheThr: 3.877 ± 0.634
2.326PheVal: 2.326 ± 0.438
0.388PheTrp: 0.388 ± 0.175
1.241PheTyr: 1.241 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
3.645GlyAla: 3.645 ± 0.546
0.465GlyCys: 0.465 ± 0.191
4.963GlyAsp: 4.963 ± 0.823
3.8GlyGlu: 3.8 ± 0.569
2.636GlyPhe: 2.636 ± 0.373
3.412GlyGly: 3.412 ± 0.775
1.008GlyHis: 1.008 ± 0.256
3.102GlyIle: 3.102 ± 0.548
3.645GlyLys: 3.645 ± 0.485
6.126GlyLeu: 6.126 ± 0.802
1.241GlyMet: 1.241 ± 0.299
3.257GlyAsn: 3.257 ± 0.665
2.094GlyPro: 2.094 ± 0.532
2.714GlyGln: 2.714 ± 0.582
4.11GlyArg: 4.11 ± 0.8
5.971GlySer: 5.971 ± 1.139
6.436GlyThr: 6.436 ± 1.735
4.73GlyVal: 4.73 ± 0.413
0.62GlyTrp: 0.62 ± 0.201
2.094GlyTyr: 2.094 ± 0.43
0.0GlyXaa: 0.0 ± 0.0
His
0.931HisAla: 0.931 ± 0.224
0.31HisCys: 0.31 ± 0.135
1.551HisAsp: 1.551 ± 0.318
0.931HisGlu: 0.931 ± 0.297
0.388HisPhe: 0.388 ± 0.191
0.853HisGly: 0.853 ± 0.27
0.543HisHis: 0.543 ± 0.234
0.775HisIle: 0.775 ± 0.273
1.318HisLys: 1.318 ± 0.288
1.241HisLeu: 1.241 ± 0.315
0.388HisMet: 0.388 ± 0.139
0.62HisAsn: 0.62 ± 0.188
0.775HisPro: 0.775 ± 0.237
1.008HisGln: 1.008 ± 0.293
0.543HisArg: 0.543 ± 0.24
0.931HisSer: 0.931 ± 0.259
1.396HisThr: 1.396 ± 0.361
0.62HisVal: 0.62 ± 0.228
0.233HisTrp: 0.233 ± 0.136
1.008HisTyr: 1.008 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
4.885IleAla: 4.885 ± 0.715
0.465IleCys: 0.465 ± 0.184
4.808IleAsp: 4.808 ± 0.528
3.567IleGlu: 3.567 ± 0.721
1.628IlePhe: 1.628 ± 0.272
3.722IleGly: 3.722 ± 0.474
1.008IleHis: 1.008 ± 0.315
3.024IleIle: 3.024 ± 0.54
3.024IleLys: 3.024 ± 0.493
3.257IleLeu: 3.257 ± 0.491
0.698IleMet: 0.698 ± 0.227
2.326IleAsn: 2.326 ± 0.521
2.404IlePro: 2.404 ± 0.409
2.094IleGln: 2.094 ± 0.276
3.179IleArg: 3.179 ± 0.477
3.489IleSer: 3.489 ± 0.461
3.8IleThr: 3.8 ± 0.593
3.645IleVal: 3.645 ± 0.629
0.31IleTrp: 0.31 ± 0.125
1.628IleTyr: 1.628 ± 0.379
0.0IleXaa: 0.0 ± 0.0
Lys
4.885LysAla: 4.885 ± 0.851
0.698LysCys: 0.698 ± 0.248
2.869LysAsp: 2.869 ± 0.538
3.877LysGlu: 3.877 ± 0.512
1.706LysPhe: 1.706 ± 0.313
3.024LysGly: 3.024 ± 0.452
0.853LysHis: 0.853 ± 0.261
4.498LysIle: 4.498 ± 0.826
2.869LysLys: 2.869 ± 0.666
4.575LysLeu: 4.575 ± 0.552
1.628LysMet: 1.628 ± 0.322
1.861LysAsn: 1.861 ± 0.297
3.877LysPro: 3.877 ± 1.102
2.714LysGln: 2.714 ± 0.534
4.498LysArg: 4.498 ± 0.603
2.636LysSer: 2.636 ± 0.492
4.265LysThr: 4.265 ± 0.596
3.024LysVal: 3.024 ± 0.49
0.698LysTrp: 0.698 ± 0.228
2.171LysTyr: 2.171 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
7.909LeuAla: 7.909 ± 0.915
0.388LeuCys: 0.388 ± 0.132
5.35LeuAsp: 5.35 ± 0.574
4.73LeuGlu: 4.73 ± 0.822
2.869LeuPhe: 2.869 ± 0.43
4.187LeuGly: 4.187 ± 0.305
1.241LeuHis: 1.241 ± 0.303
3.567LeuIle: 3.567 ± 0.652
5.35LeuLys: 5.35 ± 0.558
5.893LeuLeu: 5.893 ± 0.753
2.636LeuMet: 2.636 ± 0.397
3.877LeuAsn: 3.877 ± 0.445
4.187LeuPro: 4.187 ± 0.611
5.506LeuGln: 5.506 ± 0.75
3.412LeuArg: 3.412 ± 0.612
5.738LeuSer: 5.738 ± 0.769
5.583LeuThr: 5.583 ± 0.559
4.032LeuVal: 4.032 ± 0.574
0.543LeuTrp: 0.543 ± 0.252
2.016LeuTyr: 2.016 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
2.792MetAla: 2.792 ± 0.539
0.465MetCys: 0.465 ± 0.224
1.706MetAsp: 1.706 ± 0.387
1.318MetGlu: 1.318 ± 0.329
0.698MetPhe: 0.698 ± 0.238
1.241MetGly: 1.241 ± 0.315
0.388MetHis: 0.388 ± 0.19
1.163MetIle: 1.163 ± 0.289
1.861MetLys: 1.861 ± 0.352
1.861MetLeu: 1.861 ± 0.366
0.465MetMet: 0.465 ± 0.157
1.473MetAsn: 1.473 ± 0.324
0.775MetPro: 0.775 ± 0.261
1.241MetGln: 1.241 ± 0.312
1.628MetArg: 1.628 ± 0.365
2.636MetSer: 2.636 ± 0.397
2.404MetThr: 2.404 ± 0.404
1.241MetVal: 1.241 ± 0.287
0.31MetTrp: 0.31 ± 0.129
0.543MetTyr: 0.543 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
4.187AsnAla: 4.187 ± 0.977
0.233AsnCys: 0.233 ± 0.114
4.032AsnAsp: 4.032 ± 0.583
2.636AsnGlu: 2.636 ± 0.421
1.706AsnPhe: 1.706 ± 0.25
3.567AsnGly: 3.567 ± 0.64
0.775AsnHis: 0.775 ± 0.279
2.016AsnIle: 2.016 ± 0.384
1.551AsnLys: 1.551 ± 0.316
3.489AsnLeu: 3.489 ± 0.497
1.086AsnMet: 1.086 ± 0.263
2.792AsnAsn: 2.792 ± 0.467
2.404AsnPro: 2.404 ± 0.444
1.939AsnGln: 1.939 ± 0.431
3.179AsnArg: 3.179 ± 0.427
3.257AsnSer: 3.257 ± 0.788
2.714AsnThr: 2.714 ± 0.669
3.024AsnVal: 3.024 ± 0.484
0.543AsnTrp: 0.543 ± 0.205
1.628AsnTyr: 1.628 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
3.024ProAla: 3.024 ± 0.487
0.31ProCys: 0.31 ± 0.139
2.947ProAsp: 2.947 ± 0.498
3.024ProGlu: 3.024 ± 0.615
1.163ProPhe: 1.163 ± 0.212
3.024ProGly: 3.024 ± 0.48
0.931ProHis: 0.931 ± 0.225
2.016ProIle: 2.016 ± 0.533
2.094ProLys: 2.094 ± 0.558
2.326ProLeu: 2.326 ± 0.44
0.62ProMet: 0.62 ± 0.227
2.249ProAsn: 2.249 ± 0.326
1.939ProPro: 1.939 ± 0.669
1.939ProGln: 1.939 ± 0.404
1.939ProArg: 1.939 ± 0.545
3.102ProSer: 3.102 ± 0.483
3.489ProThr: 3.489 ± 0.453
2.481ProVal: 2.481 ± 0.502
1.008ProTrp: 1.008 ± 0.344
1.473ProTyr: 1.473 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
3.955GlnAla: 3.955 ± 0.563
0.465GlnCys: 0.465 ± 0.183
2.481GlnAsp: 2.481 ± 0.441
2.947GlnGlu: 2.947 ± 0.483
2.094GlnPhe: 2.094 ± 0.369
3.334GlnGly: 3.334 ± 0.52
0.698GlnHis: 0.698 ± 0.216
3.257GlnIle: 3.257 ± 0.557
3.257GlnLys: 3.257 ± 0.701
3.955GlnLeu: 3.955 ± 0.774
1.628GlnMet: 1.628 ± 0.506
1.318GlnAsn: 1.318 ± 0.37
1.473GlnPro: 1.473 ± 0.291
4.808GlnGln: 4.808 ± 1.201
2.947GlnArg: 2.947 ± 0.752
3.567GlnSer: 3.567 ± 0.517
3.334GlnThr: 3.334 ± 0.642
2.559GlnVal: 2.559 ± 0.378
0.233GlnTrp: 0.233 ± 0.121
1.939GlnTyr: 1.939 ± 0.379
0.0GlnXaa: 0.0 ± 0.0
Arg
4.653ArgAla: 4.653 ± 0.708
0.388ArgCys: 0.388 ± 0.177
2.636ArgAsp: 2.636 ± 0.416
3.102ArgGlu: 3.102 ± 0.558
1.783ArgPhe: 1.783 ± 0.299
3.412ArgGly: 3.412 ± 0.7
0.931ArgHis: 0.931 ± 0.293
3.179ArgIle: 3.179 ± 0.536
3.489ArgLys: 3.489 ± 0.58
5.428ArgLeu: 5.428 ± 0.629
1.473ArgMet: 1.473 ± 0.234
1.861ArgAsn: 1.861 ± 0.464
1.939ArgPro: 1.939 ± 0.408
3.102ArgGln: 3.102 ± 0.572
3.955ArgArg: 3.955 ± 0.823
3.8ArgSer: 3.8 ± 0.591
3.8ArgThr: 3.8 ± 0.514
3.024ArgVal: 3.024 ± 0.497
0.698ArgTrp: 0.698 ± 0.162
2.326ArgTyr: 2.326 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
6.436SerAla: 6.436 ± 0.757
0.543SerCys: 0.543 ± 0.213
4.42SerAsp: 4.42 ± 0.792
3.722SerGlu: 3.722 ± 0.538
3.412SerPhe: 3.412 ± 0.416
7.212SerGly: 7.212 ± 1.101
0.62SerHis: 0.62 ± 0.202
2.094SerIle: 2.094 ± 0.481
3.8SerLys: 3.8 ± 0.932
4.187SerLeu: 4.187 ± 0.527
1.551SerMet: 1.551 ± 0.336
4.42SerAsn: 4.42 ± 0.697
2.636SerPro: 2.636 ± 0.331
3.645SerGln: 3.645 ± 0.63
2.869SerArg: 2.869 ± 0.595
4.73SerSer: 4.73 ± 1.245
4.808SerThr: 4.808 ± 0.868
3.567SerVal: 3.567 ± 0.455
0.698SerTrp: 0.698 ± 0.169
1.939SerTyr: 1.939 ± 0.37
0.0SerXaa: 0.0 ± 0.0
Thr
7.754ThrAla: 7.754 ± 1.356
0.62ThrCys: 0.62 ± 0.271
4.342ThrAsp: 4.342 ± 0.501
3.877ThrGlu: 3.877 ± 0.618
2.481ThrPhe: 2.481 ± 0.55
4.885ThrGly: 4.885 ± 0.887
1.241ThrHis: 1.241 ± 0.283
4.032ThrIle: 4.032 ± 0.676
4.187ThrLys: 4.187 ± 0.635
5.661ThrLeu: 5.661 ± 0.583
1.783ThrMet: 1.783 ± 0.36
4.342ThrAsn: 4.342 ± 0.792
3.877ThrPro: 3.877 ± 0.501
2.559ThrGln: 2.559 ± 0.316
3.489ThrArg: 3.489 ± 0.572
4.42ThrSer: 4.42 ± 1.089
8.22ThrThr: 8.22 ± 1.962
5.661ThrVal: 5.661 ± 0.945
0.698ThrTrp: 0.698 ± 0.23
2.016ThrTyr: 2.016 ± 0.453
0.0ThrXaa: 0.0 ± 0.0
Val
5.893ValAla: 5.893 ± 0.749
0.233ValCys: 0.233 ± 0.122
4.653ValAsp: 4.653 ± 0.617
3.257ValGlu: 3.257 ± 0.519
1.628ValPhe: 1.628 ± 0.348
4.575ValGly: 4.575 ± 0.548
1.241ValHis: 1.241 ± 0.285
2.636ValIle: 2.636 ± 0.527
3.645ValLys: 3.645 ± 0.572
4.42ValLeu: 4.42 ± 0.585
1.473ValMet: 1.473 ± 0.313
2.481ValAsn: 2.481 ± 0.533
2.404ValPro: 2.404 ± 0.474
2.714ValGln: 2.714 ± 0.569
2.792ValArg: 2.792 ± 0.42
4.653ValSer: 4.653 ± 0.859
5.661ValThr: 5.661 ± 1.115
4.342ValVal: 4.342 ± 0.726
0.31ValTrp: 0.31 ± 0.135
1.783ValTyr: 1.783 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
0.465TrpAla: 0.465 ± 0.188
0.465TrpCys: 0.465 ± 0.223
0.465TrpAsp: 0.465 ± 0.166
0.62TrpGlu: 0.62 ± 0.195
0.543TrpPhe: 0.543 ± 0.202
0.233TrpGly: 0.233 ± 0.138
0.465TrpHis: 0.465 ± 0.171
0.543TrpIle: 0.543 ± 0.19
0.543TrpLys: 0.543 ± 0.198
1.163TrpLeu: 1.163 ± 0.276
0.233TrpMet: 0.233 ± 0.126
0.931TrpAsn: 0.931 ± 0.18
0.233TrpPro: 0.233 ± 0.12
0.233TrpGln: 0.233 ± 0.144
0.31TrpArg: 0.31 ± 0.164
1.318TrpSer: 1.318 ± 0.357
0.543TrpThr: 0.543 ± 0.211
0.853TrpVal: 0.853 ± 0.236
0.0TrpTrp: 0.0 ± 0.0
0.465TrpTyr: 0.465 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.171TyrAla: 2.171 ± 0.362
0.62TyrCys: 0.62 ± 0.229
2.636TyrAsp: 2.636 ± 0.533
2.559TyrGlu: 2.559 ± 0.508
0.931TyrPhe: 0.931 ± 0.264
2.249TyrGly: 2.249 ± 0.418
0.775TyrHis: 0.775 ± 0.272
1.241TyrIle: 1.241 ± 0.275
2.559TyrLys: 2.559 ± 0.463
2.559TyrLeu: 2.559 ± 0.352
1.241TyrMet: 1.241 ± 0.391
2.249TyrAsn: 2.249 ± 0.413
0.853TyrPro: 0.853 ± 0.23
1.706TyrGln: 1.706 ± 0.384
2.016TyrArg: 2.016 ± 0.495
1.241TyrSer: 1.241 ± 0.298
1.939TyrThr: 1.939 ± 0.333
1.163TyrVal: 1.163 ± 0.36
0.155TyrTrp: 0.155 ± 0.109
1.163TyrTyr: 1.163 ± 0.309
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12897 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski