Haloterrigena jeotgali icosahedral virus 1
Average proteome isoelectric point is 5.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G2JX99|A0A7G2JX99_9VIRU Uncharacterized protein OS=Haloterrigena jeotgali icosahedral virus 1 OX=2766528 GN=HJIV1gp28 PE=4 SV=1
MM1 pKa = 7.08 TVIGEE6 pKa = 4.13 KK7 pKa = 8.97 TATYY11 pKa = 7.58 EE12 pKa = 4.14 TLLSDD17 pKa = 5.38 LVTEE21 pKa = 4.79 LLTYY25 pKa = 10.64 SNWSDD30 pKa = 3.19 ADD32 pKa = 3.79 TYY34 pKa = 9.47 VTNDD38 pKa = 3.04 GSDD41 pKa = 3.35 TSWYY45 pKa = 10.13 NNGRR49 pKa = 11.84 VLQDD53 pKa = 3.38 SNTGTFLLMYY63 pKa = 10.27 LSGGQWHH70 pKa = 6.13 EE71 pKa = 3.92 QNGTQRR77 pKa = 11.84 HH78 pKa = 4.06 VSGIKK83 pKa = 9.92 FVISTDD89 pKa = 3.03 WDD91 pKa = 3.91 STNHH95 pKa = 5.68 YY96 pKa = 6.96 PTGYY100 pKa = 8.97 TNVGSQSGLEE110 pKa = 4.0 ANALVSNVSNDD121 pKa = 3.2 APATYY126 pKa = 10.85 DD127 pKa = 4.6 DD128 pKa = 5.49 FNATYY133 pKa = 10.53 ANHH136 pKa = 6.83 SDD138 pKa = 4.32 YY139 pKa = 11.02 DD140 pKa = 4.2 YY141 pKa = 11.61 QWFASPYY148 pKa = 9.68 GVWGSNNNLSRR159 pKa = 11.84 DD160 pKa = 4.12 TIRR163 pKa = 11.84 GTSVSYY169 pKa = 10.87 IMSVKK174 pKa = 10.6 ADD176 pKa = 3.64 GFNIGTWNSNDD187 pKa = 3.26 GNNGIASVTSYY198 pKa = 10.91 EE199 pKa = 4.13 YY200 pKa = 11.14 LSDD203 pKa = 4.85 RR204 pKa = 11.84 FFDD207 pKa = 4.37 DD208 pKa = 4.0 PGIPLIGLTRR218 pKa = 11.84 TSDD221 pKa = 2.8 IAQAAIYY228 pKa = 9.99 GFTSYY233 pKa = 11.25 SDD235 pKa = 3.37 SDD237 pKa = 3.81 STHH240 pKa = 5.68 TNRR243 pKa = 11.84 NVGFDD248 pKa = 3.55 NSPVEE253 pKa = 4.17 DD254 pKa = 4.64 ADD256 pKa = 3.45 WGIINPSSEE265 pKa = 3.98 DD266 pKa = 3.08 DD267 pKa = 3.63 TFFFRR272 pKa = 11.84 YY273 pKa = 8.19 PAAFNTTAKK282 pKa = 8.68 TVPVAYY288 pKa = 10.33 LRR290 pKa = 11.84 QTIPNDD296 pKa = 3.28 SSEE299 pKa = 4.26 GGAHH303 pKa = 7.14 GDD305 pKa = 3.79 DD306 pKa = 3.82 FTHH309 pKa = 6.77 NSTNYY314 pKa = 10.04 RR315 pKa = 11.84 VFAQSGASTSTVLSMGLRR333 pKa = 11.84 HH334 pKa = 5.89 EE335 pKa = 4.34
Molecular weight: 36.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.925
IPC2_protein 4.113
IPC_protein 4.126
Toseland 3.91
ProMoST 4.279
Dawson 4.139
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.961
Grimsley 3.821
Solomon 4.126
Lehninger 4.088
Nozaki 4.24
DTASelect 4.533
Thurlkill 3.961
EMBOSS 4.088
Sillero 4.266
Patrickios 0.782
IPC_peptide 4.126
IPC2_peptide 4.24
IPC2.peptide.svr19 4.128
Protein with the highest isoelectric point:
>tr|A0A7G2JX99|A0A7G2JX99_9VIRU Uncharacterized protein OS=Haloterrigena jeotgali icosahedral virus 1 OX=2766528 GN=HJIV1gp28 PE=4 SV=1
MM1 pKa = 7.7 GLRR4 pKa = 11.84 GDD6 pKa = 4.11 DD7 pKa = 5.86 DD8 pKa = 3.93 EE9 pKa = 5.08 PKK11 pKa = 10.02 WRR13 pKa = 11.84 LKK15 pKa = 10.05 RR16 pKa = 11.84 DD17 pKa = 3.36 RR18 pKa = 11.84 MDD20 pKa = 3.6 GGEE23 pKa = 4.21 LLVTRR28 pKa = 11.84 RR29 pKa = 11.84 DD30 pKa = 3.17 GRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 AEE36 pKa = 4.11 VIVGNLDD43 pKa = 3.36 EE44 pKa = 4.93 LVRR47 pKa = 11.84 EE48 pKa = 4.23 VLGNYY53 pKa = 7.33 PARR56 pKa = 11.84 RR57 pKa = 11.84 EE58 pKa = 4.06 ALEE61 pKa = 3.94 AAVEE65 pKa = 4.41 GWRR68 pKa = 11.84 TIPTASRR75 pKa = 11.84 RR76 pKa = 11.84 VVLRR80 pKa = 11.84 HH81 pKa = 4.25 GRR83 pKa = 11.84 GRR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 SSRR93 pKa = 11.84 RR94 pKa = 11.84 SGPAAGRR101 pKa = 11.84 RR102 pKa = 11.84 SSCRR106 pKa = 11.84 GRR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 SSRR116 pKa = 11.84 RR117 pKa = 11.84 RR118 pKa = 11.84 QDD120 pKa = 3.63 ALLEE124 pKa = 4.28 DD125 pKa = 4.93 LPDD128 pKa = 4.2 EE129 pKa = 5.2 GDD131 pKa = 4.15 DD132 pKa = 3.98 GPSRR136 pKa = 11.84 EE137 pKa = 4.15 FRR139 pKa = 11.84 AAQQDD144 pKa = 4.04 GYY146 pKa = 11.15 LRR148 pKa = 11.84 QGDD151 pKa = 3.85 SDD153 pKa = 5.0 DD154 pKa = 3.88 PDD156 pKa = 3.4 EE157 pKa = 5.98 RR158 pKa = 11.84 EE159 pKa = 4.0 GLGQFGARR167 pKa = 11.84 RR168 pKa = 11.84 SDD170 pKa = 3.44 TQALEE175 pKa = 4.66 DD176 pKa = 4.43 FEE178 pKa = 6.97 SDD180 pKa = 3.05 AA181 pKa = 5.22
Molecular weight: 20.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.487
IPC_protein 10.95
Toseland 11.067
ProMoST 11.52
Dawson 11.082
Bjellqvist 11.052
Wikipedia 11.535
Rodwell 10.716
Grimsley 11.125
Solomon 11.535
Lehninger 11.447
Nozaki 11.067
DTASelect 11.052
Thurlkill 11.067
EMBOSS 11.55
Sillero 11.067
Patrickios 10.452
IPC_peptide 11.55
IPC2_peptide 10.526
IPC2.peptide.svr19 9.685
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30
0
30
4271
61
482
142.4
15.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.342 ± 0.828
0.913 ± 0.248
9.412 ± 0.57
8.616 ± 1.029
2.81 ± 0.286
8.523 ± 0.903
1.545 ± 0.252
4.121 ± 0.312
2.458 ± 0.433
8.031 ± 0.548
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.084 ± 0.263
2.88 ± 0.612
4.893 ± 0.411
3.114 ± 0.371
7.68 ± 1.075
6.392 ± 0.677
6.532 ± 0.659
6.556 ± 0.519
1.616 ± 0.217
2.482 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here