Kingella denitrificans ATCC 33394
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2517 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F0EZJ3|F0EZJ3_9NEIS Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Kingella denitrificans ATCC 33394 OX=888741 GN=pdxH PE=3 SV=1
MM1 pKa = 7.36 YY2 pKa = 9.36 EE3 pKa = 3.83 VNRR6 pKa = 11.84 SVFIVIPRR14 pKa = 11.84 EE15 pKa = 3.98 PFWYY19 pKa = 9.04 WLNEE23 pKa = 4.07 LPDD26 pKa = 4.09 SDD28 pKa = 5.66 LGGLNLIDD36 pKa = 4.27 LQEE39 pKa = 4.57 DD40 pKa = 3.73 ANSYY44 pKa = 10.72 LVDD47 pKa = 3.42 ACEE50 pKa = 4.33 NADD53 pKa = 4.08 EE54 pKa = 4.38 LWDD57 pKa = 4.19 IIEE60 pKa = 4.29 EE61 pKa = 4.09 RR62 pKa = 11.84 VEE64 pKa = 4.15 EE65 pKa = 4.1 IFAAEE70 pKa = 3.9 LADD73 pKa = 3.33 WCADD77 pKa = 3.45 EE78 pKa = 4.68 THH80 pKa = 6.52 WPDD83 pKa = 2.98 LHH85 pKa = 8.76 LDD87 pKa = 3.88 IFRR90 pKa = 11.84 EE91 pKa = 4.06 WFDD94 pKa = 3.78 VEE96 pKa = 4.19 LSSIVTDD103 pKa = 4.55 LSSEE107 pKa = 3.98 PLQRR111 pKa = 11.84 EE112 pKa = 4.32 SFQEE116 pKa = 4.34 IYY118 pKa = 10.79 CC119 pKa = 4.3
Molecular weight: 14.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.668
IPC_protein 3.63
Toseland 3.439
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.465
Grimsley 3.35
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.478
EMBOSS 3.528
Sillero 3.745
Patrickios 0.896
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|F0F1F6|F0F1F6_9NEIS Uncharacterized protein OS=Kingella denitrificans ATCC 33394 OX=888741 GN=HMPREF9098_1941 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2517
0
2517
656618
39
1937
260.9
28.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.828 ± 0.081
1.208 ± 0.02
5.025 ± 0.032
5.726 ± 0.057
4.138 ± 0.038
7.006 ± 0.054
2.525 ± 0.028
5.549 ± 0.041
4.808 ± 0.045
10.134 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.026
3.935 ± 0.044
4.499 ± 0.036
4.999 ± 0.048
5.675 ± 0.049
5.368 ± 0.038
5.06 ± 0.037
6.561 ± 0.041
1.389 ± 0.024
2.991 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here