Mycoplasma californicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae;

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 610 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059XR69|A0A059XR69_9MOLU DnaB-related protein OS=Mycoplasma californicum OX=2113 GN=dnaB PE=4 SV=1
MM1 pKa = 7.17YY2 pKa = 10.21DD3 pKa = 3.35EE4 pKa = 5.42YY5 pKa = 10.78IIDD8 pKa = 3.55LHH10 pKa = 6.11GCNVEE15 pKa = 3.96QAISKK20 pKa = 10.23IILGLANAEE29 pKa = 4.1NNSYY33 pKa = 11.17DD34 pKa = 3.52CALIITGKK42 pKa = 8.87GTGAMKK48 pKa = 9.85TVVEE52 pKa = 4.92EE53 pKa = 4.21YY54 pKa = 10.55LHH56 pKa = 7.46SEE58 pKa = 3.84GLEE61 pKa = 3.85FEE63 pKa = 5.48LIRR66 pKa = 11.84EE67 pKa = 4.41GNYY70 pKa = 10.28LIPIYY75 pKa = 9.77YY76 pKa = 9.61QEE78 pKa = 4.55PFDD81 pKa = 3.93YY82 pKa = 11.18

Molecular weight: 9.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059XVJ0|A0A059XVJ0_9MOLU Tryptophan synthase beta chain OS=Mycoplasma californicum OX=2113 GN=trpB PE=3 SV=1
MM1 pKa = 7.84AKK3 pKa = 9.09TKK5 pKa = 10.8AGGSTKK11 pKa = 10.38NGRR14 pKa = 11.84DD15 pKa = 3.2SRR17 pKa = 11.84GQRR20 pKa = 11.84LGIKK24 pKa = 10.22LGDD27 pKa = 3.89GQFCTAGSIIFRR39 pKa = 11.84QRR41 pKa = 11.84GTKK44 pKa = 9.56IYY46 pKa = 9.54PGTNAGIGKK55 pKa = 9.8DD56 pKa = 3.4DD57 pKa = 4.43TIYY60 pKa = 11.58ALITGYY66 pKa = 11.11VKK68 pKa = 10.49FEE70 pKa = 3.79RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84NRR75 pKa = 11.84TFASVYY81 pKa = 5.73EE82 pKa = 4.32TRR84 pKa = 11.84VVTPKK89 pKa = 10.94NNN91 pKa = 3.15

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

610

0

610

230008

36

2893

377.1

42.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.039 ± 0.09

0.494 ± 0.024

5.49 ± 0.079

6.699 ± 0.093

4.979 ± 0.087

4.868 ± 0.096

1.667 ± 0.034

9.112 ± 0.09

9.921 ± 0.113

9.188 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.047

7.163 ± 0.108

2.926 ± 0.066

3.845 ± 0.072

3.213 ± 0.067

6.761 ± 0.07

5.261 ± 0.06

5.669 ± 0.071

0.96 ± 0.031

3.902 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski