Clostridium phage phiCTC2B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7S1E0|A0A0A7S1E0_9CAUD Replication terminator protein OS=Clostridium phage phiCTC2B OX=1567016 GN=phiCTC2B_18 PE=4 SV=1
MM1 pKa = 7.26NNIPEE6 pKa = 4.22CMYY9 pKa = 10.34DD10 pKa = 3.34YY11 pKa = 10.52RR12 pKa = 11.84YY13 pKa = 10.25EE14 pKa = 4.03FEE16 pKa = 4.52KK17 pKa = 10.36MQIIDD22 pKa = 3.56NCCNCDD28 pKa = 3.61CNICEE33 pKa = 4.19GEE35 pKa = 4.2EE36 pKa = 4.42YY37 pKa = 11.12YY38 pKa = 10.96DD39 pKa = 3.33IDD41 pKa = 3.76GTILCEE47 pKa = 3.83EE48 pKa = 5.17CIRR51 pKa = 11.84DD52 pKa = 3.83YY53 pKa = 11.64KK54 pKa = 9.91HH55 pKa = 5.51TAEE58 pKa = 4.36LL59 pKa = 3.79

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7S1D0|A0A0A7S1D0_9CAUD Cro/Cl family transcriptional regulator OS=Clostridium phage phiCTC2B OX=1567016 GN=phiCTC2B_03 PE=4 SV=1
MM1 pKa = 7.15YY2 pKa = 10.41KK3 pKa = 10.43LAIRR7 pKa = 11.84EE8 pKa = 3.74YY9 pKa = 10.6RR10 pKa = 11.84ILNKK14 pKa = 9.2LTQKK18 pKa = 10.85DD19 pKa = 3.17LAYY22 pKa = 10.6RR23 pKa = 11.84IGISQNYY30 pKa = 9.19LSEE33 pKa = 4.19IEE35 pKa = 4.05KK36 pKa = 10.26GKK38 pKa = 10.49YY39 pKa = 9.56DD40 pKa = 3.28IRR42 pKa = 11.84VSFLLSISNALDD54 pKa = 3.15VCPGYY59 pKa = 10.85LIRR62 pKa = 11.84CNMCKK67 pKa = 9.9CRR69 pKa = 11.84RR70 pKa = 11.84NRR72 pKa = 11.84KK73 pKa = 8.98RR74 pKa = 11.84RR75 pKa = 11.84SKK77 pKa = 11.07AA78 pKa = 3.01

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11630

43

860

176.2

20.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.082 ± 0.508

1.264 ± 0.187

5.537 ± 0.246

8.59 ± 0.575

3.353 ± 0.175

5.4 ± 0.287

1.23 ± 0.132

9.071 ± 0.306

11.35 ± 0.261

8.581 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.163

6.991 ± 0.375

2.21 ± 0.153

3.052 ± 0.178

3.465 ± 0.29

5.795 ± 0.203

5.598 ± 0.339

5.434 ± 0.272

1.04 ± 0.084

4.291 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski