Erwinia iniecta
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L7THG5|A0A0L7THG5_9GAMM Lipoprotein OS=Erwinia iniecta OX=1560201 GN=NG42_09210 PE=4 SV=1
MM1 pKa = 7.73 AYY3 pKa = 10.03 EE4 pKa = 4.21 EE5 pKa = 4.33 LLEE8 pKa = 4.18 EE9 pKa = 4.15 QRR11 pKa = 11.84 EE12 pKa = 4.19 EE13 pKa = 3.64 TRR15 pKa = 11.84 LIIEE19 pKa = 4.54 EE20 pKa = 4.2 LLEE23 pKa = 5.25 DD24 pKa = 4.98 GSDD27 pKa = 3.55 PDD29 pKa = 3.34 ALYY32 pKa = 10.6 AIEE35 pKa = 5.05 HH36 pKa = 6.49 HH37 pKa = 6.6 LSCNNFDD44 pKa = 3.91 SLEE47 pKa = 4.1 KK48 pKa = 10.56 VAVDD52 pKa = 3.67 AFKK55 pKa = 10.92 LGYY58 pKa = 9.35 EE59 pKa = 3.98 VSEE62 pKa = 4.28 PEE64 pKa = 4.5 EE65 pKa = 5.14 LDD67 pKa = 4.59 LEE69 pKa = 5.18 DD70 pKa = 3.82 GTKK73 pKa = 10.66 VMCCDD78 pKa = 4.38 IISEE82 pKa = 4.33 GALNAEE88 pKa = 4.91 LIDD91 pKa = 3.97 AQVEE95 pKa = 4.18 QLVILAAKK103 pKa = 10.37 YY104 pKa = 10.34 NVDD107 pKa = 3.26 YY108 pKa = 10.96 DD109 pKa = 3.31 GWGTYY114 pKa = 10.13 FEE116 pKa = 6.04 DD117 pKa = 5.39 PDD119 pKa = 3.93 AQDD122 pKa = 4.67 DD123 pKa = 3.9 EE124 pKa = 5.75 GEE126 pKa = 4.36 EE127 pKa = 4.27 GDD129 pKa = 5.62 EE130 pKa = 4.68 VDD132 pKa = 4.95 EE133 pKa = 5.48 DD134 pKa = 4.13 DD135 pKa = 4.04 TGVRR139 pKa = 11.84 HH140 pKa = 6.33
Molecular weight: 15.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.414
ProMoST 3.757
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.49
Rodwell 3.439
Grimsley 3.325
Solomon 3.579
Lehninger 3.528
Nozaki 3.706
DTASelect 3.872
Thurlkill 3.452
EMBOSS 3.503
Sillero 3.732
Patrickios 1.024
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.69
Protein with the highest isoelectric point:
>tr|A0A0L7T779|A0A0L7T779_9GAMM Uncharacterized protein OS=Erwinia iniecta OX=1560201 GN=NG42_05255 PE=4 SV=1
MM1 pKa = 7.54 SLPPSANIFTVSRR14 pKa = 11.84 LNTTVRR20 pKa = 11.84 KK21 pKa = 9.2 LLEE24 pKa = 4.02 MEE26 pKa = 4.85 MGQIWLSAEE35 pKa = 3.63 ISNFSQPSSGHH46 pKa = 5.4 WYY48 pKa = 7.9 FTLKK52 pKa = 10.77 DD53 pKa = 3.34 DD54 pKa = 4.55 GAQVRR59 pKa = 11.84 CAMFRR64 pKa = 11.84 NSNRR68 pKa = 11.84 RR69 pKa = 11.84 VTFRR73 pKa = 11.84 PQNGQQVLVRR83 pKa = 11.84 ASITLYY89 pKa = 10.38 EE90 pKa = 4.03 PRR92 pKa = 11.84 GDD94 pKa = 3.63 YY95 pKa = 10.67 QLIAEE100 pKa = 4.52 SMQPAGDD107 pKa = 4.14 GLLQQQFEE115 pKa = 4.25 QLKK118 pKa = 9.82 QRR120 pKa = 11.84 LAAEE124 pKa = 4.42 GLFDD128 pKa = 4.35 QQFKK132 pKa = 10.98 QPLPDD137 pKa = 3.79 PARR140 pKa = 11.84 QVGVITSATGAALHH154 pKa = 6.59 DD155 pKa = 4.26 VLRR158 pKa = 11.84 VLHH161 pKa = 6.73 RR162 pKa = 11.84 RR163 pKa = 11.84 DD164 pKa = 3.07 PSLPVIIYY172 pKa = 6.4 PTQVQGADD180 pKa = 3.3 APASLVRR187 pKa = 11.84 AIEE190 pKa = 4.09 LANQRR195 pKa = 11.84 GEE197 pKa = 4.18 CDD199 pKa = 3.34 VLIVGRR205 pKa = 11.84 GGGSLEE211 pKa = 4.7 DD212 pKa = 2.99 LWSFNDD218 pKa = 3.26 EE219 pKa = 3.92 RR220 pKa = 11.84 VARR223 pKa = 11.84 AIFASRR229 pKa = 11.84 IPVVSAVGHH238 pKa = 5.22 EE239 pKa = 4.23 TDD241 pKa = 3.12 VTIADD246 pKa = 4.4 FVADD250 pKa = 4.65 LRR252 pKa = 11.84 APTPSAAAEE261 pKa = 3.91 LVSRR265 pKa = 11.84 NQIEE269 pKa = 4.53 RR270 pKa = 11.84 LRR272 pKa = 11.84 QLQSQQQRR280 pKa = 11.84 MEE282 pKa = 4.04 MALDD286 pKa = 3.53 YY287 pKa = 11.32 YY288 pKa = 10.83 LAQKK292 pKa = 9.87 QRR294 pKa = 11.84 AFTQLHH300 pKa = 6.35 HH301 pKa = 7.37 RR302 pKa = 11.84 LQQQHH307 pKa = 5.78 PQLRR311 pKa = 11.84 LARR314 pKa = 11.84 QQTSLFRR321 pKa = 11.84 LQRR324 pKa = 11.84 RR325 pKa = 11.84 LDD327 pKa = 3.53 DD328 pKa = 4.16 AMQSRR333 pKa = 11.84 LRR335 pKa = 11.84 QSLRR339 pKa = 11.84 QQEE342 pKa = 4.51 RR343 pKa = 11.84 IEE345 pKa = 3.98 QRR347 pKa = 11.84 LAAQQPQGRR356 pKa = 11.84 LHH358 pKa = 7.48 RR359 pKa = 11.84 AQQQLQQWQYY369 pKa = 11.16 RR370 pKa = 11.84 LQQGMQSQLSRR381 pKa = 11.84 DD382 pKa = 3.44 KK383 pKa = 11.18 QRR385 pKa = 11.84 FGTLAAQLEE394 pKa = 5.05 GVSPLATLARR404 pKa = 11.84 GFSVTTAGDD413 pKa = 3.73 GQVVRR418 pKa = 11.84 KK419 pKa = 7.74 TRR421 pKa = 11.84 QIKK424 pKa = 10.62 RR425 pKa = 11.84 GDD427 pKa = 3.65 TLKK430 pKa = 10.02 TRR432 pKa = 11.84 LDD434 pKa = 4.22 DD435 pKa = 3.93 GWVEE439 pKa = 4.22 SQVTRR444 pKa = 11.84 VSAIKK449 pKa = 10.52 KK450 pKa = 6.81 KK451 pKa = 8.64 TRR453 pKa = 3.14
Molecular weight: 51.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.516
IPC_protein 10.511
Toseland 10.657
ProMoST 10.599
Dawson 10.745
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 10.804
Grimsley 10.804
Solomon 10.921
Lehninger 10.877
Nozaki 10.643
DTASelect 10.511
Thurlkill 10.657
EMBOSS 11.082
Sillero 10.687
Patrickios 10.482
IPC_peptide 10.921
IPC2_peptide 9.531
IPC2.peptide.svr19 8.822
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4072
33
4105
1321614
23
4133
322.0
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.892 ± 0.044
0.983 ± 0.013
5.222 ± 0.029
5.447 ± 0.035
3.876 ± 0.027
7.266 ± 0.038
2.246 ± 0.018
5.769 ± 0.034
4.032 ± 0.032
11.173 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.018
3.739 ± 0.028
4.459 ± 0.025
5.024 ± 0.039
5.707 ± 0.029
6.255 ± 0.031
5.096 ± 0.024
6.846 ± 0.031
1.515 ± 0.016
2.737 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here