Morelia spilota papillomavirus 1
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3DRD2|G3DRD2_9PAPI Replication protein E1 OS=Morelia spilota papillomavirus 1 OX=1081054 GN=E1 PE=3 SV=1
MM1 pKa = 7.5 MIGPKK6 pKa = 10.06 LDD8 pKa = 3.44 VDD10 pKa = 4.26 LLCYY14 pKa = 10.43 EE15 pKa = 5.39 DD16 pKa = 4.24 LTSPNPDD23 pKa = 3.05 EE24 pKa = 4.71 AAIPAPPPSPSHH36 pKa = 6.73 PDD38 pKa = 2.99 LRR40 pKa = 11.84 AYY42 pKa = 9.56 TIDD45 pKa = 4.61 LGCGYY50 pKa = 8.1 CTKK53 pKa = 10.22 PLRR56 pKa = 11.84 FVTVATAEE64 pKa = 4.49 CISLFNRR71 pKa = 11.84 LLLLDD76 pKa = 5.39 LYY78 pKa = 9.98 ILCPEE83 pKa = 4.57 CVDD86 pKa = 4.81 EE87 pKa = 6.41 RR88 pKa = 11.84 DD89 pKa = 2.93 LDD91 pKa = 3.93 YY92 pKa = 11.46 YY93 pKa = 11.49 YY94 pKa = 11.33 GGG96 pKa = 3.81
Molecular weight: 10.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.984
IPC2_protein 4.05
IPC_protein 3.986
Toseland 3.783
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.694
Solomon 3.973
Lehninger 3.935
Nozaki 4.113
DTASelect 4.342
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.113
Patrickios 0.121
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|G3DRD4|G3DRD4_9PAPI E4 (Fragment) OS=Morelia spilota papillomavirus 1 OX=1081054 PE=4 SV=1
MM1 pKa = 6.55 EE2 pKa = 4.8 TLRR5 pKa = 11.84 KK6 pKa = 9.77 RR7 pKa = 11.84 LDD9 pKa = 3.48 AVQEE13 pKa = 4.0 ALLDD17 pKa = 3.66 IYY19 pKa = 9.82 EE20 pKa = 4.37 TAEE23 pKa = 4.04 NTLEE27 pKa = 4.07 TQIKK31 pKa = 8.02 HH32 pKa = 4.81 WKK34 pKa = 9.55 LVRR37 pKa = 11.84 QEE39 pKa = 3.65 QTLLYY44 pKa = 9.86 FARR47 pKa = 11.84 QQGLSHH53 pKa = 7.09 IGLQTVPALQISEE66 pKa = 4.46 SKK68 pKa = 10.63 AKK70 pKa = 9.88 EE71 pKa = 3.92 AIEE74 pKa = 3.76 IQLYY78 pKa = 10.58 LEE80 pKa = 4.43 SLQNSKK86 pKa = 11.17 YY87 pKa = 10.59 GDD89 pKa = 4.26 EE90 pKa = 4.19 PWTMQEE96 pKa = 3.75 TSALTFFAVPSRR108 pKa = 11.84 TFKK111 pKa = 10.72 KK112 pKa = 10.38 GPTTVVVQFEE122 pKa = 4.33 QHH124 pKa = 4.59 TQSYY128 pKa = 6.6 TTWTYY133 pKa = 11.42 LYY135 pKa = 9.61 IQTDD139 pKa = 3.07 TDD141 pKa = 2.87 MWTKK145 pKa = 10.75 YY146 pKa = 9.76 VGKK149 pKa = 10.31 VSNEE153 pKa = 3.29 GAYY156 pKa = 10.24 YY157 pKa = 10.46 SAGGGLKK164 pKa = 9.17 TFYY167 pKa = 10.48 ISFSAEE173 pKa = 3.39 AKK175 pKa = 10.27 KK176 pKa = 11.11 YY177 pKa = 8.72 NTDD180 pKa = 2.72 NWTLVYY186 pKa = 10.23 KK187 pKa = 9.34 QLPVLTSSITPLQSPTGTTDD207 pKa = 3.03 TAEE210 pKa = 4.09 TQPAGGRR217 pKa = 11.84 PKK219 pKa = 10.08 RR220 pKa = 11.84 RR221 pKa = 11.84 YY222 pKa = 9.09 GRR224 pKa = 11.84 RR225 pKa = 11.84 SEE227 pKa = 4.11 SPRR230 pKa = 11.84 PRR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 RR235 pKa = 11.84 EE236 pKa = 3.84 DD237 pKa = 3.35 QEE239 pKa = 4.05 ASSPGGVPVAPEE251 pKa = 3.99 DD252 pKa = 3.42 VGSRR256 pKa = 11.84 NRR258 pKa = 11.84 TVEE261 pKa = 3.89 AKK263 pKa = 10.21 RR264 pKa = 11.84 SSRR267 pKa = 11.84 LARR270 pKa = 11.84 LLDD273 pKa = 3.74 EE274 pKa = 5.62 ARR276 pKa = 11.84 DD277 pKa = 3.79 PPIVVLKK284 pKa = 10.59 GNPNSLKK291 pKa = 10.05 CLRR294 pKa = 11.84 YY295 pKa = 9.43 RR296 pKa = 11.84 LRR298 pKa = 11.84 HH299 pKa = 5.09 QYY301 pKa = 10.66 SKK303 pKa = 10.67 EE304 pKa = 3.8 FQHH307 pKa = 7.38 ISTTFQWVDD316 pKa = 3.36 TVDD319 pKa = 3.35 SARR322 pKa = 11.84 LGRR325 pKa = 11.84 GRR327 pKa = 11.84 MLVMFTDD334 pKa = 4.59 DD335 pKa = 3.39 AQRR338 pKa = 11.84 MHH340 pKa = 6.86 FLKK343 pKa = 10.56 HH344 pKa = 5.26 VPLPKK349 pKa = 10.26 HH350 pKa = 4.32 ITAFKK355 pKa = 10.08 GQLDD359 pKa = 4.86 GII361 pKa = 4.5
Molecular weight: 41.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.841
IPC2_protein 8.96
IPC_protein 8.931
Toseland 9.487
ProMoST 9.311
Dawson 9.794
Bjellqvist 9.502
Wikipedia 9.999
Rodwell 10.014
Grimsley 9.897
Solomon 9.823
Lehninger 9.78
Nozaki 9.487
DTASelect 9.502
Thurlkill 9.604
EMBOSS 9.926
Sillero 9.706
Patrickios 4.952
IPC_peptide 9.809
IPC2_peptide 8.039
IPC2.peptide.svr19 7.911
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2240
88
596
320.0
36.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.295 ± 0.447
1.741 ± 0.701
6.161 ± 0.702
6.652 ± 0.723
5.045 ± 0.534
5.313 ± 0.553
1.786 ± 0.313
5.536 ± 0.53
5.134 ± 1.044
9.643 ± 1.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.696 ± 0.181
4.063 ± 0.465
5.804 ± 0.831
4.42 ± 0.705
6.027 ± 0.717
7.187 ± 0.629
6.875 ± 0.679
5.982 ± 0.714
1.205 ± 0.351
3.438 ± 0.479
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here