African pouched rat arterivirus
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5JFS1|A0A0B5JFS1_9NIDO Nucleocapsid protein OS=African pouched rat arterivirus OX=1965064 GN=ORF7 PE=3 SV=1
MM1 pKa = 7.44 GANIPSVHH9 pKa = 6.67 LLIMCCCLHH18 pKa = 6.63 AVSGDD23 pKa = 3.48 DD24 pKa = 3.82 TEE26 pKa = 4.96 NSLVSCFSFPEE37 pKa = 4.28 SNKK40 pKa = 10.3 GSLQLSATVSTCPAKK55 pKa = 10.56 YY56 pKa = 10.26 LRR58 pKa = 11.84 ATTGEE63 pKa = 4.14 EE64 pKa = 4.01 GAKK67 pKa = 10.73 ANGVNAKK74 pKa = 10.18 AKK76 pKa = 9.44 GGFYY80 pKa = 10.68 VSVNPKK86 pKa = 10.17 HH87 pKa = 5.92 PWCGYY92 pKa = 8.8 HH93 pKa = 6.84 HH94 pKa = 7.04 AVDD97 pKa = 3.97 NPGTEE102 pKa = 3.86 YY103 pKa = 10.72 QYY105 pKa = 11.48 RR106 pKa = 11.84 DD107 pKa = 3.33 TTLCGAIMLVGYY119 pKa = 9.62 YY120 pKa = 10.35 DD121 pKa = 3.22 HH122 pKa = 7.42 VYY124 pKa = 10.98 NFVCTGHH131 pKa = 6.13 HH132 pKa = 5.48 TANNSFTYY140 pKa = 9.6 EE141 pKa = 4.21 LPPHH145 pKa = 6.15 QIVLPMVLQVATYY158 pKa = 10.19 VAILHH163 pKa = 5.84 PQLFGFEE170 pKa = 4.56 NITAVVAHH178 pKa = 6.5 EE179 pKa = 3.94 NFICFEE185 pKa = 4.38 HH186 pKa = 6.81 GGNPVNITKK195 pKa = 10.06 AHH197 pKa = 6.85 PDD199 pKa = 2.96 SGFRR203 pKa = 11.84 STLYY207 pKa = 10.55 DD208 pKa = 5.71 LEE210 pKa = 4.61 VQWQYY215 pKa = 11.81 LEE217 pKa = 3.93 WVRR220 pKa = 11.84 PLFSCWLVMCISLTLRR236 pKa = 11.84 RR237 pKa = 11.84 SSALLAEE244 pKa = 4.58 RR245 pKa = 4.66
Molecular weight: 27.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.697
IPC2_protein 5.792
IPC_protein 5.881
Toseland 6.262
ProMoST 6.3
Dawson 6.211
Bjellqvist 6.198
Wikipedia 6.237
Rodwell 6.211
Grimsley 6.389
Solomon 6.211
Lehninger 6.211
Nozaki 6.504
DTASelect 6.693
Thurlkill 6.722
EMBOSS 6.678
Sillero 6.605
Patrickios 0.54
IPC_peptide 6.237
IPC2_peptide 6.62
IPC2.peptide.svr19 6.472
Protein with the highest isoelectric point:
>tr|A0A0B5JKI0|A0A0B5JKI0_9NIDO GP4 glycoprotein OS=African pouched rat arterivirus OX=1965064 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.58 SGPIGFSHH9 pKa = 7.51 ALFDD13 pKa = 3.66 QLTRR17 pKa = 11.84 AGMPRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 9.35 AHH26 pKa = 6.88 KK27 pKa = 9.61 NPQPAHH33 pKa = 5.84 QDD35 pKa = 3.28 LVKK38 pKa = 10.81 ALSSLLNKK46 pKa = 10.05 GQGPKK51 pKa = 9.98 KK52 pKa = 9.64 GQAGSGSRR60 pKa = 11.84 QKK62 pKa = 10.54 RR63 pKa = 11.84 HH64 pKa = 5.53 EE65 pKa = 4.22 NALQYY70 pKa = 10.89 PLAQPGDD77 pKa = 4.15 LRR79 pKa = 11.84 RR80 pKa = 11.84 MLKK83 pKa = 8.6 PTSAPMVRR91 pKa = 11.84 SALLSAFNSGGGILVGDD108 pKa = 4.29 ADD110 pKa = 3.97 GKK112 pKa = 9.39 VTFTCDD118 pKa = 2.66 IQVPPRR124 pKa = 11.84 FIHH127 pKa = 6.12 QPKK130 pKa = 9.62 QPSEE134 pKa = 3.9
Molecular weight: 14.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.259
IPC2_protein 9.648
IPC_protein 9.955
Toseland 10.906
ProMoST 10.438
Dawson 10.95
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.359
Grimsley 10.965
Solomon 11.052
Lehninger 11.038
Nozaki 10.877
DTASelect 10.57
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.877
Patrickios 11.111
IPC_peptide 11.067
IPC2_peptide 9.194
IPC2.peptide.svr19 8.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
1
10
7352
59
3714
735.2
80.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.399 ± 0.398
3.509 ± 0.251
4.312 ± 0.596
4.067 ± 0.315
3.904 ± 0.548
7.331 ± 0.436
2.979 ± 0.386
3.808 ± 0.309
3.904 ± 0.249
10.365 ± 0.488
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.823 ± 0.381
3.346 ± 0.28
6.27 ± 0.806
2.924 ± 0.293
5.169 ± 0.283
7.141 ± 0.485
5.794 ± 0.273
9.168 ± 0.539
1.469 ± 0.141
3.319 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here