Hubei tombus-like virus 31

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGW3|A0A1L3KGW3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 31 OX=1923279 PE=4 SV=1
MM1 pKa = 6.81NTNTTTHH8 pKa = 5.81EE9 pKa = 4.18RR10 pKa = 11.84TLKK13 pKa = 10.65SGIVVKK19 pKa = 7.56FTRR22 pKa = 11.84SPGTLLSKK30 pKa = 10.11DD31 pKa = 3.55TEE33 pKa = 4.57TEE35 pKa = 4.0GTIVTDD41 pKa = 3.66GWEE44 pKa = 4.09SSSRR48 pKa = 11.84SQTVSEE54 pKa = 4.3PTNLAAALEE63 pKa = 4.22PVLTKK68 pKa = 10.69GVVVASVVRR77 pKa = 11.84PQRR80 pKa = 11.84QAPEE84 pKa = 4.45SPVLTLRR91 pKa = 11.84AIRR94 pKa = 11.84RR95 pKa = 11.84GTRR98 pKa = 11.84AGRR101 pKa = 11.84SVRR104 pKa = 11.84RR105 pKa = 11.84SAVFKK110 pKa = 10.42EE111 pKa = 4.1LQDD114 pKa = 4.22YY115 pKa = 8.39PLSWAVIEE123 pKa = 4.05QLRR126 pKa = 11.84ILATEE131 pKa = 3.83ICLGKK136 pKa = 10.03EE137 pKa = 3.65LNVEE141 pKa = 3.79NFRR144 pKa = 11.84IFVRR148 pKa = 11.84KK149 pKa = 9.86FEE151 pKa = 5.07DD152 pKa = 3.51KK153 pKa = 10.32LTLTEE158 pKa = 4.16VYY160 pKa = 10.28YY161 pKa = 10.7SQSRR165 pKa = 11.84LDD167 pKa = 3.69SVEE170 pKa = 4.91DD171 pKa = 3.76ILWDD175 pKa = 3.61EE176 pKa = 4.71APAHH180 pKa = 5.75AVAVIKK186 pKa = 10.8ISDD189 pKa = 3.97DD190 pKa = 3.37RR191 pKa = 11.84LSATRR196 pKa = 11.84SFRR199 pKa = 11.84LGSALEE205 pKa = 4.13ASLPAAAYY213 pKa = 10.2RR214 pKa = 11.84DD215 pKa = 3.88VANTASAVDD224 pKa = 3.42DD225 pKa = 4.1MFRR228 pKa = 11.84EE229 pKa = 4.89LYY231 pKa = 10.43LLHH234 pKa = 6.56NPGVIEE240 pKa = 4.88RR241 pKa = 11.84RR242 pKa = 11.84NIQQGRR248 pKa = 11.84IEE250 pKa = 4.36YY251 pKa = 10.73GSDD254 pKa = 2.41SWLWRR259 pKa = 11.84GLEE262 pKa = 3.96SVYY265 pKa = 10.82YY266 pKa = 10.59GSAARR271 pKa = 11.84WNWLVGKK278 pKa = 9.72QLAVPTAPQCC288 pKa = 3.99

Molecular weight: 32.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGW3|A0A1L3KGW3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 31 OX=1923279 PE=4 SV=1
MM1 pKa = 7.46NARR4 pKa = 11.84LALSQRR10 pKa = 11.84LEE12 pKa = 3.93IPANVALRR20 pKa = 11.84KK21 pKa = 10.0VEE23 pKa = 3.86INKK26 pKa = 9.59DD27 pKa = 3.08HH28 pKa = 5.41THH30 pKa = 5.26WVEE33 pKa = 3.68LYY35 pKa = 10.73KK36 pKa = 10.32IVSKK40 pKa = 10.5LARR43 pKa = 11.84AVSHH47 pKa = 6.54IRR49 pKa = 11.84PLTPAEE55 pKa = 3.98FLKK58 pKa = 10.17TANSRR63 pKa = 11.84QRR65 pKa = 11.84PVWEE69 pKa = 3.57RR70 pKa = 11.84ALRR73 pKa = 11.84QDD75 pKa = 3.5RR76 pKa = 11.84RR77 pKa = 11.84FRR79 pKa = 11.84PSWMTYY85 pKa = 10.36DD86 pKa = 3.52GFVKK90 pKa = 9.93IEE92 pKa = 3.9KK93 pKa = 9.24YY94 pKa = 10.52SYY96 pKa = 7.92YY97 pKa = 10.77EE98 pKa = 3.65KK99 pKa = 10.32MNRR102 pKa = 11.84IPRR105 pKa = 11.84LILPPSDD112 pKa = 3.19HH113 pKa = 6.98AKK115 pKa = 10.38VVMGMHH121 pKa = 6.88IKK123 pKa = 10.31PIEE126 pKa = 3.92KK127 pKa = 9.77HH128 pKa = 5.43LKK130 pKa = 9.3EE131 pKa = 4.24VVGPGNVYY139 pKa = 10.42PFMAKK144 pKa = 10.02GMSSRR149 pKa = 11.84QLAEE153 pKa = 3.42RR154 pKa = 11.84FMRR157 pKa = 11.84MAEE160 pKa = 3.96QFKK163 pKa = 10.35RR164 pKa = 11.84PTFISIDD171 pKa = 3.52MSKK174 pKa = 10.55CDD176 pKa = 3.52STIGPRR182 pKa = 11.84LKK184 pKa = 10.51RR185 pKa = 11.84LEE187 pKa = 3.95NMVFTNYY194 pKa = 9.74HH195 pKa = 5.67SSSDD199 pKa = 3.02HH200 pKa = 5.46RR201 pKa = 11.84RR202 pKa = 11.84CMKK205 pKa = 10.45EE206 pKa = 3.75GEE208 pKa = 4.22KK209 pKa = 10.18TFMKK213 pKa = 10.35VRR215 pKa = 11.84LHH217 pKa = 6.72CNDD220 pKa = 2.78GTTEE224 pKa = 3.87TSEE227 pKa = 4.1IPQCRR232 pKa = 11.84ASGTAHH238 pKa = 6.53TGAGNTVLVYY248 pKa = 10.24GASAVVLRR256 pKa = 11.84GINNEE261 pKa = 3.95VFSNGDD267 pKa = 3.25DD268 pKa = 3.64TILIVEE274 pKa = 4.72ASDD277 pKa = 3.27SAEE280 pKa = 3.93VIRR283 pKa = 11.84RR284 pKa = 11.84INDD287 pKa = 3.05HH288 pKa = 6.73EE289 pKa = 4.43YY290 pKa = 10.72SVFGFDD296 pKa = 4.35VRR298 pKa = 11.84IEE300 pKa = 4.0QVATDD305 pKa = 3.53IEE307 pKa = 4.64DD308 pKa = 4.01VFWCQCYY315 pKa = 8.25YY316 pKa = 9.84TVRR319 pKa = 11.84KK320 pKa = 10.04DD321 pKa = 3.38GPIWIRR327 pKa = 11.84DD328 pKa = 3.59YY329 pKa = 11.43RR330 pKa = 11.84KK331 pKa = 9.69VLQTILSNEE340 pKa = 4.31NYY342 pKa = 10.34GSPNWLSYY350 pKa = 11.17LSTICLGEE358 pKa = 4.23GSTNPGQPIVAPLVSTILKK377 pKa = 9.66LRR379 pKa = 11.84HH380 pKa = 5.51KK381 pKa = 10.57RR382 pKa = 11.84MRR384 pKa = 11.84LPNEE388 pKa = 3.81NQTTRR393 pKa = 11.84RR394 pKa = 11.84WEE396 pKa = 3.99LEE398 pKa = 3.68GCPSVEE404 pKa = 3.99EE405 pKa = 4.37LDD407 pKa = 5.04LEE409 pKa = 4.64VTAQDD414 pKa = 3.51RR415 pKa = 11.84ACFHH419 pKa = 6.3ARR421 pKa = 11.84YY422 pKa = 9.42GISPVEE428 pKa = 4.11QLRR431 pKa = 11.84MEE433 pKa = 4.52AVNNLALRR441 pKa = 11.84GLGDD445 pKa = 3.42GPVRR449 pKa = 11.84HH450 pKa = 6.55EE451 pKa = 3.62YY452 pKa = 7.52WQNRR456 pKa = 11.84IHH458 pKa = 7.02PVGG461 pKa = 3.38

Molecular weight: 52.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1165

288

461

388.3

43.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.124 ± 0.805

1.545 ± 0.296

4.12 ± 0.24

5.579 ± 1.34

3.777 ± 0.819

7.039 ± 1.294

1.974 ± 0.595

5.322 ± 0.368

4.549 ± 0.37

7.639 ± 0.958

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.597

4.807 ± 0.628

4.549 ± 0.307

3.262 ± 0.107

6.953 ± 1.744

8.412 ± 0.929

7.639 ± 1.317

8.155 ± 0.325

1.459 ± 0.482

3.691 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski