Yellowstone lake phycodnavirus 1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0YN66|A0A0P0YN66_9PHYC Uncharacterized protein OS=Yellowstone lake phycodnavirus 1 OX=1586713 PE=4 SV=1
MM1 pKa = 7.9GINDD5 pKa = 3.93EE6 pKa = 4.33YY7 pKa = 11.7SRR9 pKa = 11.84DD10 pKa = 3.25AYY12 pKa = 11.09YY13 pKa = 10.6EE14 pKa = 3.98YY15 pKa = 11.16NAVVDD20 pKa = 4.05QDD22 pKa = 4.35DD23 pKa = 4.43DD24 pKa = 3.99QDD26 pKa = 3.88EE27 pKa = 4.66FDD29 pKa = 5.64LEE31 pKa = 4.22LHH33 pKa = 6.47PEE35 pKa = 4.21DD36 pKa = 3.87WQDD39 pKa = 3.19MYY41 pKa = 11.55SQEE44 pKa = 5.27LLDD47 pKa = 3.6GWMHH51 pKa = 6.23LRR53 pKa = 11.84QYY55 pKa = 10.84IDD57 pKa = 3.46EE58 pKa = 4.67NYY60 pKa = 9.99MKK62 pKa = 9.33ITVGFPTFVGFVLTPHH78 pKa = 5.14VWYY81 pKa = 9.4TEE83 pKa = 3.99DD84 pKa = 5.96QPALWQQCIWNDD96 pKa = 2.74ISKK99 pKa = 10.36IGVITDD105 pKa = 3.98RR106 pKa = 11.84VCPEE110 pKa = 3.74NFYY113 pKa = 11.3AWVNNYY119 pKa = 9.07VEE121 pKa = 4.59YY122 pKa = 10.57II123 pKa = 3.58

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0YMG1|A0A0P0YMG1_9PHYC Uncharacterized protein OS=Yellowstone lake phycodnavirus 1 OX=1586713 PE=4 SV=1
MM1 pKa = 7.4TPPLRR6 pKa = 11.84RR7 pKa = 11.84ASSAPARR14 pKa = 11.84LGRR17 pKa = 11.84NNRR20 pKa = 11.84VSPSQVNRR28 pKa = 11.84LALMLANMSLHH39 pKa = 5.33VSPMNVNNNARR50 pKa = 11.84PMNINNPRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84SPTVRR67 pKa = 11.84RR68 pKa = 11.84SPGGARR74 pKa = 11.84KK75 pKa = 9.35RR76 pKa = 11.84ARR78 pKa = 11.84RR79 pKa = 3.57

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

248

0

248

56779

55

1644

228.9

25.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.462 ± 0.244

1.582 ± 0.113

4.9 ± 0.216

5.085 ± 0.274

4.493 ± 0.119

7.207 ± 0.43

1.8 ± 0.097

6.055 ± 0.137

6.115 ± 0.317

8.07 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.131

5.96 ± 0.299

5.104 ± 0.185

3.565 ± 0.125

4.991 ± 0.216

6.46 ± 0.232

6.7 ± 0.305

6.668 ± 0.13

1.421 ± 0.064

3.739 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski