Australian Anopheles totivirus
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223PIK2|A0A223PIK2_9VIRU RNA-directed RNA polymerase OS=Australian Anopheles totivirus OX=2026601 PE=3 SV=1
MM1 pKa = 7.4 TEE3 pKa = 3.6 VDD5 pKa = 3.72 GSQVADD11 pKa = 3.37 SSQSVDD17 pKa = 2.87 RR18 pKa = 11.84 VAFVGDD24 pKa = 3.49 EE25 pKa = 4.27 GFTALAEE32 pKa = 4.44 SVGEE36 pKa = 3.97 IQKK39 pKa = 8.61 ATNFAGSWEE48 pKa = 4.43 VVGSSGAKK56 pKa = 9.64 KK57 pKa = 9.33 PAAGGPGRR65 pKa = 11.84 RR66 pKa = 11.84 VTRR69 pKa = 11.84 QALSGVQSHH78 pKa = 6.84 SNDD81 pKa = 3.01 IPKK84 pKa = 10.13 NDD86 pKa = 3.78 PDD88 pKa = 4.02 QRR90 pKa = 11.84 GAGNGGHH97 pKa = 5.96 SRR99 pKa = 11.84 PGGGNGGGGGSRR111 pKa = 11.84 GDD113 pKa = 3.91 GPSGGHH119 pKa = 5.99 GPSGDD124 pKa = 3.44 GHH126 pKa = 7.04 DD127 pKa = 4.26 GGGGGGGGGHH137 pKa = 7.54 PGNGGDD143 pKa = 3.7 PHH145 pKa = 7.14 PGRR148 pKa = 11.84 SVGNGGPSGTPSASTGASALLAKK171 pKa = 10.66 LFTEE175 pKa = 4.73 PLRR178 pKa = 11.84 DD179 pKa = 3.76 PVPSGTPFLPCNTEE193 pKa = 3.88 EE194 pKa = 4.21 VVSEE198 pKa = 4.37 RR199 pKa = 11.84 EE200 pKa = 4.32 GKK202 pKa = 7.95 GTQYY206 pKa = 11.2 SPVMWADD213 pKa = 3.18 PLIPINVTIGSVHH226 pKa = 5.67 SAINKK231 pKa = 9.09 LPGVGVSVNNKK242 pKa = 9.49 LDD244 pKa = 3.54 MVEE247 pKa = 4.35 PLLTEE252 pKa = 3.5 QGMMLKK258 pKa = 10.3 SLIDD262 pKa = 3.79 SEE264 pKa = 5.24 SNPSLLRR271 pKa = 11.84 SMMPVKK277 pKa = 10.44 GMKK280 pKa = 10.02 DD281 pKa = 2.84 ISALISLMEE290 pKa = 3.78 TSRR293 pKa = 11.84 RR294 pKa = 11.84 SGIQAMSDD302 pKa = 3.41 EE303 pKa = 4.86 APLIRR308 pKa = 11.84 LGLLMHH314 pKa = 6.88 CLAPVEE320 pKa = 3.96 WMAYY324 pKa = 7.78 STSCVQNSVANGYY337 pKa = 11.04 ADD339 pKa = 3.25 VAGAKK344 pKa = 9.93 RR345 pKa = 11.84 SWTSAPTNSTATQIGTDD362 pKa = 3.26 AGSSNLYY369 pKa = 9.21 WRR371 pKa = 11.84 TLSQYY376 pKa = 10.85 ARR378 pKa = 11.84 LVNGSGTKK386 pKa = 9.95 HH387 pKa = 4.93 DD388 pKa = 4.24 TIYY391 pKa = 10.99 NSKK394 pKa = 10.06 QEE396 pKa = 4.27 EE397 pKa = 4.65 VPVDD401 pKa = 3.75 SVKK404 pKa = 10.8 FIPVKK409 pKa = 10.14 MSWRR413 pKa = 11.84 GQSWLGPYY421 pKa = 9.82 ILAHH425 pKa = 6.57 TSTKK429 pKa = 7.73 WWNHH433 pKa = 3.69 SVQVEE438 pKa = 4.64 VPVKK442 pKa = 10.46 IHH444 pKa = 6.08 DD445 pKa = 3.93 QKK447 pKa = 11.79 SSTNKK452 pKa = 9.63 MKK454 pKa = 9.27 ITCMPKK460 pKa = 9.49 AATVYY465 pKa = 10.48 VPGSYY470 pKa = 10.29 KK471 pKa = 10.37 YY472 pKa = 8.96 ICLVITDD479 pKa = 3.86 VVEE482 pKa = 4.55 ASFPTTEE489 pKa = 4.09 AFYY492 pKa = 10.62 IGNSYY497 pKa = 9.62 KK498 pKa = 10.74 ALYY501 pKa = 9.99 NGEE504 pKa = 4.14 FGFGKK509 pKa = 9.74 IGHH512 pKa = 7.03 KK513 pKa = 9.83 ILGRR517 pKa = 11.84 NNEE520 pKa = 4.1 SPARR524 pKa = 11.84 AASMSDD530 pKa = 3.79 CVAAWRR536 pKa = 11.84 VICKK540 pKa = 10.37 GLLSHH545 pKa = 6.75 VNPGAIEE552 pKa = 3.84 VRR554 pKa = 11.84 LAILSTSRR562 pKa = 11.84 FAGFSVWTDD571 pKa = 3.05 EE572 pKa = 4.93 AKK574 pKa = 10.41 PKK576 pKa = 10.69 KK577 pKa = 9.06 KK578 pKa = 9.21 TSDD581 pKa = 3.39 VEE583 pKa = 4.22 EE584 pKa = 4.93 EE585 pKa = 4.29 YY586 pKa = 11.27 SSLLRR591 pKa = 11.84 DD592 pKa = 3.32 VGMDD596 pKa = 3.35 EE597 pKa = 5.81 SEE599 pKa = 4.91 APDD602 pKa = 5.69 DD603 pKa = 3.94 FDD605 pKa = 4.8 GWLEE609 pKa = 3.95 LLAEE613 pKa = 4.76 ASAQIGEE620 pKa = 4.16 AWDD623 pKa = 3.36 ARR625 pKa = 11.84 GVVTVEE631 pKa = 5.23 KK632 pKa = 10.35 IRR634 pKa = 11.84 LMKK637 pKa = 10.4 KK638 pKa = 10.1 AMSIKK643 pKa = 10.21 FGKK646 pKa = 9.52 QLEE649 pKa = 4.29 AEE651 pKa = 4.29 EE652 pKa = 4.83 DD653 pKa = 3.42 VDD655 pKa = 4.57 HH656 pKa = 7.44 IGGATCADD664 pKa = 4.06 PKK666 pKa = 11.08 SKK668 pKa = 9.43 VTIGDD673 pKa = 3.46 HH674 pKa = 7.45 DD675 pKa = 4.44 LPDD678 pKa = 3.42 WFSQYY683 pKa = 9.11 NTTATMPDD691 pKa = 2.9 RR692 pKa = 11.84 VKK694 pKa = 11.1 YY695 pKa = 5.74 YY696 pKa = 8.77 TWWRR700 pKa = 11.84 TDD702 pKa = 3.19 PLHH705 pKa = 6.96 NYY707 pKa = 8.8 SQALISCTDD716 pKa = 3.36 GEE718 pKa = 4.53 KK719 pKa = 10.22 PALEE723 pKa = 4.12 KK724 pKa = 10.97 FEE726 pKa = 4.55 SGEE729 pKa = 3.81 VQYY732 pKa = 11.12 EE733 pKa = 4.04 VTEE736 pKa = 4.4 ANDD739 pKa = 4.49 LIRR742 pKa = 11.84 ILSYY746 pKa = 10.88 SGVYY750 pKa = 8.99 MDD752 pKa = 5.09 NSSCAGAITEE762 pKa = 5.76 AIDD765 pKa = 3.13 ILNRR769 pKa = 11.84 SVGFGSLVLAVTEE782 pKa = 4.45 LWRR785 pKa = 11.84 ATCGLPLLDD794 pKa = 4.21 YY795 pKa = 11.29 NRR797 pKa = 11.84 ISTSWKK803 pKa = 10.55 LNDD806 pKa = 3.38 PQMRR810 pKa = 11.84 KK811 pKa = 9.89 LISLVTLGFAVEE823 pKa = 4.84 SPNTTNTAWTDD834 pKa = 2.96 TDD836 pKa = 3.99 FEE838 pKa = 4.31 IVKK841 pKa = 9.73 VVKK844 pKa = 9.86 EE845 pKa = 4.04 AMGITDD851 pKa = 5.07 DD852 pKa = 3.41 KK853 pKa = 11.23 WVDD856 pKa = 2.69 HH857 pKa = 6.95 WYY859 pKa = 10.89 GGVVPWWFVQAVLTKK874 pKa = 10.52 FGGQMAVRR882 pKa = 11.84 TKK884 pKa = 10.77 EE885 pKa = 3.91 PTSVKK890 pKa = 10.74 LNVDD894 pKa = 4.1 DD895 pKa = 5.36 DD896 pKa = 3.96 WLDD899 pKa = 3.44 EE900 pKa = 4.17 VGYY903 pKa = 10.04 HH904 pKa = 5.91 IKK906 pKa = 10.76 ANEE909 pKa = 4.08 SFATDD914 pKa = 3.34 ISVLTSSIMYY924 pKa = 7.57 EE925 pKa = 3.93 KK926 pKa = 10.35 KK927 pKa = 8.95 VQRR930 pKa = 11.84 RR931 pKa = 11.84 ASPFFTILTPTKK943 pKa = 9.45 GAHH946 pKa = 4.93 EE947 pKa = 4.56 MKK949 pKa = 10.6 VHH951 pKa = 5.01 WTSWYY956 pKa = 9.67 YY957 pKa = 11.57 NFVNDD962 pKa = 4.63 LPKK965 pKa = 10.5 SGLRR969 pKa = 11.84 RR970 pKa = 11.84 RR971 pKa = 11.84 MKK973 pKa = 9.38 VTAPDD978 pKa = 3.36 FDD980 pKa = 4.11 GVVEE984 pKa = 4.46 LNTMVFPDD992 pKa = 3.22 SRR994 pKa = 11.84 RR995 pKa = 11.84 FKK997 pKa = 10.88 GYY999 pKa = 10.46 ASPEE1003 pKa = 3.82 AFIDD1007 pKa = 3.57 GTNRR1011 pKa = 11.84 YY1012 pKa = 8.69 LLPLDD1017 pKa = 3.75 HH1018 pKa = 7.17 CVMSGLTWPDD1028 pKa = 3.75 PFVDD1032 pKa = 3.41 WLKK1035 pKa = 10.8 QGLVAIEE1042 pKa = 3.91 PHH1044 pKa = 6.39 LLNLDD1049 pKa = 3.45 VVGAVGAALGHH1060 pKa = 5.0 VNKK1063 pKa = 10.21 TIIDD1067 pKa = 3.67 WLNEE1071 pKa = 4.1 KK1072 pKa = 9.76 IHH1074 pKa = 6.48 GSGSFRR1080 pKa = 11.84 DD1081 pKa = 3.75 EE1082 pKa = 3.95 SDD1084 pKa = 2.76
Molecular weight: 117.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.436
IPC2_protein 5.372
IPC_protein 5.397
Toseland 5.652
ProMoST 5.626
Dawson 5.55
Bjellqvist 5.614
Wikipedia 5.512
Rodwell 5.499
Grimsley 5.741
Solomon 5.55
Lehninger 5.525
Nozaki 5.753
DTASelect 5.944
Thurlkill 5.817
EMBOSS 5.779
Sillero 5.842
Patrickios 4.19
IPC_peptide 5.563
IPC2_peptide 5.842
IPC2.peptide.svr19 5.824
Protein with the highest isoelectric point:
>tr|A0A223PIK2|A0A223PIK2_9VIRU RNA-directed RNA polymerase OS=Australian Anopheles totivirus OX=2026601 PE=3 SV=1
MM1 pKa = 7.8 ADD3 pKa = 3.09 VSLLHH8 pKa = 6.66 LKK10 pKa = 10.54 FSSIVAKK17 pKa = 10.03 WDD19 pKa = 3.47 GPKK22 pKa = 10.45 NFSNVRR28 pKa = 11.84 LVVKK32 pKa = 9.8 TSSPSDD38 pKa = 3.34 DD39 pKa = 3.33 RR40 pKa = 11.84 VEE42 pKa = 3.97 VSPLGGNTKK51 pKa = 9.76 YY52 pKa = 10.39 VHH54 pKa = 6.68 SNVEE58 pKa = 3.58 YY59 pKa = 10.4 SAIRR63 pKa = 11.84 DD64 pKa = 3.7 RR65 pKa = 11.84 LKK67 pKa = 10.97 SKK69 pKa = 10.43 HH70 pKa = 6.08 CVEE73 pKa = 4.53 ADD75 pKa = 2.99 GFSFFKK81 pKa = 10.66 NYY83 pKa = 10.59 NPFALFKK90 pKa = 10.19 LQRR93 pKa = 11.84 WSKK96 pKa = 10.44 NDD98 pKa = 3.3 AGLGIHH104 pKa = 5.53 STFMKK109 pKa = 10.44 PLLFYY114 pKa = 10.86 YY115 pKa = 8.78 KK116 pKa = 10.24 TLGCVKK122 pKa = 8.68 NTEE125 pKa = 4.06 MTRR128 pKa = 11.84 DD129 pKa = 3.6 LLGGIISYY137 pKa = 11.19 VNDD140 pKa = 3.43 LTSRR144 pKa = 11.84 AVGNGMFDD152 pKa = 3.98 YY153 pKa = 10.39 IDD155 pKa = 3.68 DD156 pKa = 4.39 VIRR159 pKa = 11.84 QFLAEE164 pKa = 4.02 VDD166 pKa = 3.36 KK167 pKa = 10.76 LTFVLCQQNAGNSLVYY183 pKa = 10.53 SSLHH187 pKa = 5.69 GLSTLGGPRR196 pKa = 11.84 GWTEE200 pKa = 3.96 DD201 pKa = 3.43 SVLASLKK208 pKa = 10.71 DD209 pKa = 3.39 WVTGEE214 pKa = 4.21 RR215 pKa = 11.84 KK216 pKa = 9.17 FPYY219 pKa = 10.49 EE220 pKa = 3.53 NDD222 pKa = 3.14 RR223 pKa = 11.84 FLNAKK228 pKa = 9.25 LDD230 pKa = 3.55 SWIDD234 pKa = 3.15 EE235 pKa = 3.87 WSRR238 pKa = 11.84 NIKK241 pKa = 10.45 NDD243 pKa = 3.6 DD244 pKa = 3.56 LSFSEE249 pKa = 5.01 FVSDD253 pKa = 3.61 PMRR256 pKa = 11.84 WATGGGAKK264 pKa = 9.65 KK265 pKa = 10.2 KK266 pKa = 10.38 QMNIRR271 pKa = 11.84 GRR273 pKa = 11.84 QVDD276 pKa = 4.13 GRR278 pKa = 11.84 NKK280 pKa = 9.45 WFWALSGLSKK290 pKa = 11.43 GEE292 pKa = 4.33 DD293 pKa = 3.68 LYY295 pKa = 11.5 QVGLDD300 pKa = 3.7 EE301 pKa = 5.3 GNNAQVALKK310 pKa = 10.34 EE311 pKa = 4.06 EE312 pKa = 4.27 AKK314 pKa = 8.78 TRR316 pKa = 11.84 CVITTPQASYY326 pKa = 11.06 LRR328 pKa = 11.84 QCYY331 pKa = 8.7 MLYY334 pKa = 10.56 RR335 pKa = 11.84 FGNPSFLRR343 pKa = 11.84 STLSSPHH350 pKa = 6.17 LVSEE354 pKa = 4.67 LSRR357 pKa = 11.84 SRR359 pKa = 11.84 KK360 pKa = 9.59 DD361 pKa = 3.12 HH362 pKa = 6.83 FICIDD367 pKa = 3.36 SSSFDD372 pKa = 3.36 HH373 pKa = 6.77 SVSKK377 pKa = 10.52 KK378 pKa = 7.89 WVLSVLQRR386 pKa = 11.84 MADD389 pKa = 3.38 RR390 pKa = 11.84 CRR392 pKa = 11.84 GEE394 pKa = 3.95 LRR396 pKa = 11.84 DD397 pKa = 4.04 VIISEE402 pKa = 4.15 MDD404 pKa = 3.37 SLRR407 pKa = 11.84 DD408 pKa = 3.39 MSISYY413 pKa = 10.22 NNHH416 pKa = 3.64 VLKK419 pKa = 11.14 YY420 pKa = 10.38 EE421 pKa = 4.12 NGLLSGWRR429 pKa = 11.84 MTSLLGSMLSALVCEE444 pKa = 5.58 FINHH448 pKa = 4.54 SMMWKK453 pKa = 10.16 LPYY456 pKa = 9.57 IVQGDD461 pKa = 4.86 DD462 pKa = 3.73 IIMMCPRR469 pKa = 11.84 KK470 pKa = 10.16 VSAEE474 pKa = 3.88 RR475 pKa = 11.84 VLEE478 pKa = 4.21 CCSRR482 pKa = 11.84 FGIITNKK489 pKa = 10.12 KK490 pKa = 7.38 KK491 pKa = 7.48 TTIGRR496 pKa = 11.84 FGEE499 pKa = 4.17 FLKK502 pKa = 10.82 YY503 pKa = 9.69 RR504 pKa = 11.84 YY505 pKa = 10.16 GYY507 pKa = 11.18 GRR509 pKa = 11.84 VQGYY513 pKa = 8.52 AARR516 pKa = 11.84 SVRR519 pKa = 11.84 SIFYY523 pKa = 10.5 ANPWLDD529 pKa = 3.42 STAVGAASEE538 pKa = 4.62 VSGKK542 pKa = 7.87 WWTLLSRR549 pKa = 11.84 LMNSHH554 pKa = 6.54 NGGFKK559 pKa = 10.65 DD560 pKa = 3.93 EE561 pKa = 5.3 EE562 pKa = 4.21 SLEE565 pKa = 3.95 WFMNNIASDD574 pKa = 3.46 VGGWLGGRR582 pKa = 11.84 VSRR585 pKa = 11.84 SALLDD590 pKa = 4.49 AIKK593 pKa = 9.92 TPVSLGGLGVFEE605 pKa = 4.25 TADD608 pKa = 3.05 INLANNNGFITKK620 pKa = 8.45 ITSIVQEE627 pKa = 4.5 DD628 pKa = 4.09 SFGDD632 pKa = 3.75 SKK634 pKa = 10.96 FVSLFAPDD642 pKa = 3.56 VLSKK646 pKa = 10.61 VGKK649 pKa = 9.28 VSTRR653 pKa = 11.84 FVDD656 pKa = 3.73 VKK658 pKa = 10.55 KK659 pKa = 10.33 IAMNFGRR666 pKa = 11.84 DD667 pKa = 3.3 LTEE670 pKa = 4.43 FKK672 pKa = 10.62 SKK674 pKa = 10.39 YY675 pKa = 9.59 HH676 pKa = 6.46 SIVNTAGRR684 pKa = 11.84 VVFDD688 pKa = 3.35 SGSNIFRR695 pKa = 11.84 TVLSEE700 pKa = 3.72 IASCRR705 pKa = 11.84 NYY707 pKa = 10.37 PPIVDD712 pKa = 4.1 RR713 pKa = 11.84 LLSSVKK719 pKa = 9.93 GKK721 pKa = 10.13 CSSIVRR727 pKa = 11.84 PRR729 pKa = 11.84 FLRR732 pKa = 11.84 NSNRR736 pKa = 11.84 WYY738 pKa = 10.37 DD739 pKa = 3.51 VAKK742 pKa = 9.53 WLTEE746 pKa = 3.62 ITLKK750 pKa = 10.74 ADD752 pKa = 3.73 CPPSLFVDD760 pKa = 3.65 TRR762 pKa = 11.84 YY763 pKa = 11.14 DD764 pKa = 3.57 NEE766 pKa = 4.42 LVQSLAGVAVTMFMNLSNVTARR788 pKa = 11.84 SEE790 pKa = 4.0 YY791 pKa = 10.24 LISVFALFRR800 pKa = 11.84 FGHH803 pKa = 5.4 TKK805 pKa = 10.64 CILHH809 pKa = 6.75 ALL811 pKa = 3.91
Molecular weight: 91.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.579
IPC2_protein 8.463
IPC_protein 8.317
Toseland 8.887
ProMoST 8.887
Dawson 9.282
Bjellqvist 9.209
Wikipedia 9.428
Rodwell 9.458
Grimsley 9.268
Solomon 9.326
Lehninger 9.282
Nozaki 9.355
DTASelect 9.077
Thurlkill 9.194
EMBOSS 9.428
Sillero 9.37
Patrickios 4.698
IPC_peptide 9.311
IPC2_peptide 7.936
IPC2.peptide.svr19 7.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1895
811
1084
947.5
104.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.491 ± 0.732
1.425 ± 0.262
6.174 ± 0.081
4.802 ± 0.452
3.958 ± 0.904
8.865 ± 1.056
2.058 ± 0.204
4.644 ± 0.102
5.858 ± 0.265
8.338 ± 0.94
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.03
4.644 ± 0.481
4.169 ± 0.973
2.164 ± 0.193
5.013 ± 0.861
9.868 ± 0.605
5.488 ± 0.646
7.968 ± 0.123
2.533 ± 0.117
2.902 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here