Australian Anopheles totivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A223PIK2|A0A223PIK2_9VIRU RNA-directed RNA polymerase OS=Australian Anopheles totivirus OX=2026601 PE=3 SV=1
MM1 pKa = 7.4TEE3 pKa = 3.6VDD5 pKa = 3.72GSQVADD11 pKa = 3.37SSQSVDD17 pKa = 2.87RR18 pKa = 11.84VAFVGDD24 pKa = 3.49EE25 pKa = 4.27GFTALAEE32 pKa = 4.44SVGEE36 pKa = 3.97IQKK39 pKa = 8.61ATNFAGSWEE48 pKa = 4.43VVGSSGAKK56 pKa = 9.64KK57 pKa = 9.33PAAGGPGRR65 pKa = 11.84RR66 pKa = 11.84VTRR69 pKa = 11.84QALSGVQSHH78 pKa = 6.84SNDD81 pKa = 3.01IPKK84 pKa = 10.13NDD86 pKa = 3.78PDD88 pKa = 4.02QRR90 pKa = 11.84GAGNGGHH97 pKa = 5.96SRR99 pKa = 11.84PGGGNGGGGGSRR111 pKa = 11.84GDD113 pKa = 3.91GPSGGHH119 pKa = 5.99GPSGDD124 pKa = 3.44GHH126 pKa = 7.04DD127 pKa = 4.26GGGGGGGGGHH137 pKa = 7.54PGNGGDD143 pKa = 3.7PHH145 pKa = 7.14PGRR148 pKa = 11.84SVGNGGPSGTPSASTGASALLAKK171 pKa = 10.66LFTEE175 pKa = 4.73PLRR178 pKa = 11.84DD179 pKa = 3.76PVPSGTPFLPCNTEE193 pKa = 3.88EE194 pKa = 4.21VVSEE198 pKa = 4.37RR199 pKa = 11.84EE200 pKa = 4.32GKK202 pKa = 7.95GTQYY206 pKa = 11.2SPVMWADD213 pKa = 3.18PLIPINVTIGSVHH226 pKa = 5.67SAINKK231 pKa = 9.09LPGVGVSVNNKK242 pKa = 9.49LDD244 pKa = 3.54MVEE247 pKa = 4.35PLLTEE252 pKa = 3.5QGMMLKK258 pKa = 10.3SLIDD262 pKa = 3.79SEE264 pKa = 5.24SNPSLLRR271 pKa = 11.84SMMPVKK277 pKa = 10.44GMKK280 pKa = 10.02DD281 pKa = 2.84ISALISLMEE290 pKa = 3.78TSRR293 pKa = 11.84RR294 pKa = 11.84SGIQAMSDD302 pKa = 3.41EE303 pKa = 4.86APLIRR308 pKa = 11.84LGLLMHH314 pKa = 6.88CLAPVEE320 pKa = 3.96WMAYY324 pKa = 7.78STSCVQNSVANGYY337 pKa = 11.04ADD339 pKa = 3.25VAGAKK344 pKa = 9.93RR345 pKa = 11.84SWTSAPTNSTATQIGTDD362 pKa = 3.26AGSSNLYY369 pKa = 9.21WRR371 pKa = 11.84TLSQYY376 pKa = 10.85ARR378 pKa = 11.84LVNGSGTKK386 pKa = 9.95HH387 pKa = 4.93DD388 pKa = 4.24TIYY391 pKa = 10.99NSKK394 pKa = 10.06QEE396 pKa = 4.27EE397 pKa = 4.65VPVDD401 pKa = 3.75SVKK404 pKa = 10.8FIPVKK409 pKa = 10.14MSWRR413 pKa = 11.84GQSWLGPYY421 pKa = 9.82ILAHH425 pKa = 6.57TSTKK429 pKa = 7.73WWNHH433 pKa = 3.69SVQVEE438 pKa = 4.64VPVKK442 pKa = 10.46IHH444 pKa = 6.08DD445 pKa = 3.93QKK447 pKa = 11.79SSTNKK452 pKa = 9.63MKK454 pKa = 9.27ITCMPKK460 pKa = 9.49AATVYY465 pKa = 10.48VPGSYY470 pKa = 10.29KK471 pKa = 10.37YY472 pKa = 8.96ICLVITDD479 pKa = 3.86VVEE482 pKa = 4.55ASFPTTEE489 pKa = 4.09AFYY492 pKa = 10.62IGNSYY497 pKa = 9.62KK498 pKa = 10.74ALYY501 pKa = 9.99NGEE504 pKa = 4.14FGFGKK509 pKa = 9.74IGHH512 pKa = 7.03KK513 pKa = 9.83ILGRR517 pKa = 11.84NNEE520 pKa = 4.1SPARR524 pKa = 11.84AASMSDD530 pKa = 3.79CVAAWRR536 pKa = 11.84VICKK540 pKa = 10.37GLLSHH545 pKa = 6.75VNPGAIEE552 pKa = 3.84VRR554 pKa = 11.84LAILSTSRR562 pKa = 11.84FAGFSVWTDD571 pKa = 3.05EE572 pKa = 4.93AKK574 pKa = 10.41PKK576 pKa = 10.69KK577 pKa = 9.06KK578 pKa = 9.21TSDD581 pKa = 3.39VEE583 pKa = 4.22EE584 pKa = 4.93EE585 pKa = 4.29YY586 pKa = 11.27SSLLRR591 pKa = 11.84DD592 pKa = 3.32VGMDD596 pKa = 3.35EE597 pKa = 5.81SEE599 pKa = 4.91APDD602 pKa = 5.69DD603 pKa = 3.94FDD605 pKa = 4.8GWLEE609 pKa = 3.95LLAEE613 pKa = 4.76ASAQIGEE620 pKa = 4.16AWDD623 pKa = 3.36ARR625 pKa = 11.84GVVTVEE631 pKa = 5.23KK632 pKa = 10.35IRR634 pKa = 11.84LMKK637 pKa = 10.4KK638 pKa = 10.1AMSIKK643 pKa = 10.21FGKK646 pKa = 9.52QLEE649 pKa = 4.29AEE651 pKa = 4.29EE652 pKa = 4.83DD653 pKa = 3.42VDD655 pKa = 4.57HH656 pKa = 7.44IGGATCADD664 pKa = 4.06PKK666 pKa = 11.08SKK668 pKa = 9.43VTIGDD673 pKa = 3.46HH674 pKa = 7.45DD675 pKa = 4.44LPDD678 pKa = 3.42WFSQYY683 pKa = 9.11NTTATMPDD691 pKa = 2.9RR692 pKa = 11.84VKK694 pKa = 11.1YY695 pKa = 5.74YY696 pKa = 8.77TWWRR700 pKa = 11.84TDD702 pKa = 3.19PLHH705 pKa = 6.96NYY707 pKa = 8.8SQALISCTDD716 pKa = 3.36GEE718 pKa = 4.53KK719 pKa = 10.22PALEE723 pKa = 4.12KK724 pKa = 10.97FEE726 pKa = 4.55SGEE729 pKa = 3.81VQYY732 pKa = 11.12EE733 pKa = 4.04VTEE736 pKa = 4.4ANDD739 pKa = 4.49LIRR742 pKa = 11.84ILSYY746 pKa = 10.88SGVYY750 pKa = 8.99MDD752 pKa = 5.09NSSCAGAITEE762 pKa = 5.76AIDD765 pKa = 3.13ILNRR769 pKa = 11.84SVGFGSLVLAVTEE782 pKa = 4.45LWRR785 pKa = 11.84ATCGLPLLDD794 pKa = 4.21YY795 pKa = 11.29NRR797 pKa = 11.84ISTSWKK803 pKa = 10.55LNDD806 pKa = 3.38PQMRR810 pKa = 11.84KK811 pKa = 9.89LISLVTLGFAVEE823 pKa = 4.84SPNTTNTAWTDD834 pKa = 2.96TDD836 pKa = 3.99FEE838 pKa = 4.31IVKK841 pKa = 9.73VVKK844 pKa = 9.86EE845 pKa = 4.04AMGITDD851 pKa = 5.07DD852 pKa = 3.41KK853 pKa = 11.23WVDD856 pKa = 2.69HH857 pKa = 6.95WYY859 pKa = 10.89GGVVPWWFVQAVLTKK874 pKa = 10.52FGGQMAVRR882 pKa = 11.84TKK884 pKa = 10.77EE885 pKa = 3.91PTSVKK890 pKa = 10.74LNVDD894 pKa = 4.1DD895 pKa = 5.36DD896 pKa = 3.96WLDD899 pKa = 3.44EE900 pKa = 4.17VGYY903 pKa = 10.04HH904 pKa = 5.91IKK906 pKa = 10.76ANEE909 pKa = 4.08SFATDD914 pKa = 3.34ISVLTSSIMYY924 pKa = 7.57EE925 pKa = 3.93KK926 pKa = 10.35KK927 pKa = 8.95VQRR930 pKa = 11.84RR931 pKa = 11.84ASPFFTILTPTKK943 pKa = 9.45GAHH946 pKa = 4.93EE947 pKa = 4.56MKK949 pKa = 10.6VHH951 pKa = 5.01WTSWYY956 pKa = 9.67YY957 pKa = 11.57NFVNDD962 pKa = 4.63LPKK965 pKa = 10.5SGLRR969 pKa = 11.84RR970 pKa = 11.84RR971 pKa = 11.84MKK973 pKa = 9.38VTAPDD978 pKa = 3.36FDD980 pKa = 4.11GVVEE984 pKa = 4.46LNTMVFPDD992 pKa = 3.22SRR994 pKa = 11.84RR995 pKa = 11.84FKK997 pKa = 10.88GYY999 pKa = 10.46ASPEE1003 pKa = 3.82AFIDD1007 pKa = 3.57GTNRR1011 pKa = 11.84YY1012 pKa = 8.69LLPLDD1017 pKa = 3.75HH1018 pKa = 7.17CVMSGLTWPDD1028 pKa = 3.75PFVDD1032 pKa = 3.41WLKK1035 pKa = 10.8QGLVAIEE1042 pKa = 3.91PHH1044 pKa = 6.39LLNLDD1049 pKa = 3.45VVGAVGAALGHH1060 pKa = 5.0VNKK1063 pKa = 10.21TIIDD1067 pKa = 3.67WLNEE1071 pKa = 4.1KK1072 pKa = 9.76IHH1074 pKa = 6.48GSGSFRR1080 pKa = 11.84DD1081 pKa = 3.75EE1082 pKa = 3.95SDD1084 pKa = 2.76

Molecular weight:
117.44 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A223PIK2|A0A223PIK2_9VIRU RNA-directed RNA polymerase OS=Australian Anopheles totivirus OX=2026601 PE=3 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.09VSLLHH8 pKa = 6.66LKK10 pKa = 10.54FSSIVAKK17 pKa = 10.03WDD19 pKa = 3.47GPKK22 pKa = 10.45NFSNVRR28 pKa = 11.84LVVKK32 pKa = 9.8TSSPSDD38 pKa = 3.34DD39 pKa = 3.33RR40 pKa = 11.84VEE42 pKa = 3.97VSPLGGNTKK51 pKa = 9.76YY52 pKa = 10.39VHH54 pKa = 6.68SNVEE58 pKa = 3.58YY59 pKa = 10.4SAIRR63 pKa = 11.84DD64 pKa = 3.7RR65 pKa = 11.84LKK67 pKa = 10.97SKK69 pKa = 10.43HH70 pKa = 6.08CVEE73 pKa = 4.53ADD75 pKa = 2.99GFSFFKK81 pKa = 10.66NYY83 pKa = 10.59NPFALFKK90 pKa = 10.19LQRR93 pKa = 11.84WSKK96 pKa = 10.44NDD98 pKa = 3.3AGLGIHH104 pKa = 5.53STFMKK109 pKa = 10.44PLLFYY114 pKa = 10.86YY115 pKa = 8.78KK116 pKa = 10.24TLGCVKK122 pKa = 8.68NTEE125 pKa = 4.06MTRR128 pKa = 11.84DD129 pKa = 3.6LLGGIISYY137 pKa = 11.19VNDD140 pKa = 3.43LTSRR144 pKa = 11.84AVGNGMFDD152 pKa = 3.98YY153 pKa = 10.39IDD155 pKa = 3.68DD156 pKa = 4.39VIRR159 pKa = 11.84QFLAEE164 pKa = 4.02VDD166 pKa = 3.36KK167 pKa = 10.76LTFVLCQQNAGNSLVYY183 pKa = 10.53SSLHH187 pKa = 5.69GLSTLGGPRR196 pKa = 11.84GWTEE200 pKa = 3.96DD201 pKa = 3.43SVLASLKK208 pKa = 10.71DD209 pKa = 3.39WVTGEE214 pKa = 4.21RR215 pKa = 11.84KK216 pKa = 9.17FPYY219 pKa = 10.49EE220 pKa = 3.53NDD222 pKa = 3.14RR223 pKa = 11.84FLNAKK228 pKa = 9.25LDD230 pKa = 3.55SWIDD234 pKa = 3.15EE235 pKa = 3.87WSRR238 pKa = 11.84NIKK241 pKa = 10.45NDD243 pKa = 3.6DD244 pKa = 3.56LSFSEE249 pKa = 5.01FVSDD253 pKa = 3.61PMRR256 pKa = 11.84WATGGGAKK264 pKa = 9.65KK265 pKa = 10.2KK266 pKa = 10.38QMNIRR271 pKa = 11.84GRR273 pKa = 11.84QVDD276 pKa = 4.13GRR278 pKa = 11.84NKK280 pKa = 9.45WFWALSGLSKK290 pKa = 11.43GEE292 pKa = 4.33DD293 pKa = 3.68LYY295 pKa = 11.5QVGLDD300 pKa = 3.7EE301 pKa = 5.3GNNAQVALKK310 pKa = 10.34EE311 pKa = 4.06EE312 pKa = 4.27AKK314 pKa = 8.78TRR316 pKa = 11.84CVITTPQASYY326 pKa = 11.06LRR328 pKa = 11.84QCYY331 pKa = 8.7MLYY334 pKa = 10.56RR335 pKa = 11.84FGNPSFLRR343 pKa = 11.84STLSSPHH350 pKa = 6.17LVSEE354 pKa = 4.67LSRR357 pKa = 11.84SRR359 pKa = 11.84KK360 pKa = 9.59DD361 pKa = 3.12HH362 pKa = 6.83FICIDD367 pKa = 3.36SSSFDD372 pKa = 3.36HH373 pKa = 6.77SVSKK377 pKa = 10.52KK378 pKa = 7.89WVLSVLQRR386 pKa = 11.84MADD389 pKa = 3.38RR390 pKa = 11.84CRR392 pKa = 11.84GEE394 pKa = 3.95LRR396 pKa = 11.84DD397 pKa = 4.04VIISEE402 pKa = 4.15MDD404 pKa = 3.37SLRR407 pKa = 11.84DD408 pKa = 3.39MSISYY413 pKa = 10.22NNHH416 pKa = 3.64VLKK419 pKa = 11.14YY420 pKa = 10.38EE421 pKa = 4.12NGLLSGWRR429 pKa = 11.84MTSLLGSMLSALVCEE444 pKa = 5.58FINHH448 pKa = 4.54SMMWKK453 pKa = 10.16LPYY456 pKa = 9.57IVQGDD461 pKa = 4.86DD462 pKa = 3.73IIMMCPRR469 pKa = 11.84KK470 pKa = 10.16VSAEE474 pKa = 3.88RR475 pKa = 11.84VLEE478 pKa = 4.21CCSRR482 pKa = 11.84FGIITNKK489 pKa = 10.12KK490 pKa = 7.38KK491 pKa = 7.48TTIGRR496 pKa = 11.84FGEE499 pKa = 4.17FLKK502 pKa = 10.82YY503 pKa = 9.69RR504 pKa = 11.84YY505 pKa = 10.16GYY507 pKa = 11.18GRR509 pKa = 11.84VQGYY513 pKa = 8.52AARR516 pKa = 11.84SVRR519 pKa = 11.84SIFYY523 pKa = 10.5ANPWLDD529 pKa = 3.42STAVGAASEE538 pKa = 4.62VSGKK542 pKa = 7.87WWTLLSRR549 pKa = 11.84LMNSHH554 pKa = 6.54NGGFKK559 pKa = 10.65DD560 pKa = 3.93EE561 pKa = 5.3EE562 pKa = 4.21SLEE565 pKa = 3.95WFMNNIASDD574 pKa = 3.46VGGWLGGRR582 pKa = 11.84VSRR585 pKa = 11.84SALLDD590 pKa = 4.49AIKK593 pKa = 9.92TPVSLGGLGVFEE605 pKa = 4.25TADD608 pKa = 3.05INLANNNGFITKK620 pKa = 8.45ITSIVQEE627 pKa = 4.5DD628 pKa = 4.09SFGDD632 pKa = 3.75SKK634 pKa = 10.96FVSLFAPDD642 pKa = 3.56VLSKK646 pKa = 10.61VGKK649 pKa = 9.28VSTRR653 pKa = 11.84FVDD656 pKa = 3.73VKK658 pKa = 10.55KK659 pKa = 10.33IAMNFGRR666 pKa = 11.84DD667 pKa = 3.3LTEE670 pKa = 4.43FKK672 pKa = 10.62SKK674 pKa = 10.39YY675 pKa = 9.59HH676 pKa = 6.46SIVNTAGRR684 pKa = 11.84VVFDD688 pKa = 3.35SGSNIFRR695 pKa = 11.84TVLSEE700 pKa = 3.72IASCRR705 pKa = 11.84NYY707 pKa = 10.37PPIVDD712 pKa = 4.1RR713 pKa = 11.84LLSSVKK719 pKa = 9.93GKK721 pKa = 10.13CSSIVRR727 pKa = 11.84PRR729 pKa = 11.84FLRR732 pKa = 11.84NSNRR736 pKa = 11.84WYY738 pKa = 10.37DD739 pKa = 3.51VAKK742 pKa = 9.53WLTEE746 pKa = 3.62ITLKK750 pKa = 10.74ADD752 pKa = 3.73CPPSLFVDD760 pKa = 3.65TRR762 pKa = 11.84YY763 pKa = 11.14DD764 pKa = 3.57NEE766 pKa = 4.42LVQSLAGVAVTMFMNLSNVTARR788 pKa = 11.84SEE790 pKa = 4.0YY791 pKa = 10.24LISVFALFRR800 pKa = 11.84FGHH803 pKa = 5.4TKK805 pKa = 10.64CILHH809 pKa = 6.75ALL811 pKa = 3.91

Molecular weight:
91.42 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1895

811

1084

947.5

104.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.491 ± 0.732

1.425 ± 0.262

6.174 ± 0.081

4.802 ± 0.452

3.958 ± 0.904

8.865 ± 1.056

2.058 ± 0.204

4.644 ± 0.102

5.858 ± 0.265

8.338 ± 0.94

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.03

4.644 ± 0.481

4.169 ± 0.973

2.164 ± 0.193

5.013 ± 0.861

9.868 ± 0.605

5.488 ± 0.646

7.968 ± 0.123

2.533 ± 0.117

2.902 ± 0.187

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski