Natrarchaeobaculum aegyptiacum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natrarchaeobaculum

Average proteome isoelectric point is 4.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2HVW8|A0A2Z2HVW8_9EURY DUF357 domain-containing protein OS=Natrarchaeobaculum aegyptiacum OX=745377 GN=B1756_17640 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 4.86LTWHH6 pKa = 6.23GHH8 pKa = 4.48STWHH12 pKa = 4.8VTVGGTDD19 pKa = 3.66LLIDD23 pKa = 4.15PFFDD27 pKa = 3.84NPKK30 pKa = 9.57TDD32 pKa = 4.65LEE34 pKa = 4.43PADD37 pKa = 4.65VDD39 pKa = 3.95DD40 pKa = 5.39PDD42 pKa = 4.47YY43 pKa = 11.81VLLTHH48 pKa = 6.39GHH50 pKa = 7.0ADD52 pKa = 4.13HH53 pKa = 7.06IAHH56 pKa = 6.9AGEE59 pKa = 4.54FSDD62 pKa = 3.89ATLVATPEE70 pKa = 4.08LVSYY74 pKa = 10.0CQEE77 pKa = 3.68EE78 pKa = 4.66FGFEE82 pKa = 4.05DD83 pKa = 3.69AVGGMGMNLGGTVDD97 pKa = 4.39CGDD100 pKa = 3.57ATVTMVRR107 pKa = 11.84ADD109 pKa = 3.57HH110 pKa = 6.68TNGIMTEE117 pKa = 3.94NDD119 pKa = 3.09ASGGMPAGFVIADD132 pKa = 4.39GDD134 pKa = 4.03PTDD137 pKa = 5.23DD138 pKa = 6.1DD139 pKa = 3.94STTVYY144 pKa = 10.85NAGDD148 pKa = 3.65TALMSEE154 pKa = 4.47MKK156 pKa = 10.54DD157 pKa = 3.43VIGDD161 pKa = 3.88HH162 pKa = 6.89LAPDD166 pKa = 3.87AAIVPIGDD174 pKa = 4.27HH175 pKa = 5.57FTMGPTQAGVAVDD188 pKa = 3.37WTGADD193 pKa = 3.06HH194 pKa = 7.75AFPQHH199 pKa = 6.83YY200 pKa = 8.02DD201 pKa = 3.22TFPPIEE207 pKa = 4.18QDD209 pKa = 3.34PADD212 pKa = 4.07FEE214 pKa = 4.95AAVSDD219 pKa = 4.27AEE221 pKa = 4.43VHH223 pKa = 5.87VLEE226 pKa = 5.26ADD228 pKa = 3.67EE229 pKa = 4.64PFEE232 pKa = 4.37LL233 pKa = 5.69

Molecular weight: 24.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2I1A7|A0A2Z2I1A7_9EURY Uncharacterized protein OS=Natrarchaeobaculum aegyptiacum OX=745377 GN=B1756_18615 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84FGSDD6 pKa = 2.96RR7 pKa = 11.84SPSVAAVADD16 pKa = 4.12GARR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84PRR23 pKa = 11.84PRR25 pKa = 11.84ATDD28 pKa = 3.37EE29 pKa = 4.6GNGDD33 pKa = 3.54PVRR36 pKa = 11.84RR37 pKa = 11.84TGVCSSDD44 pKa = 2.98GVTPQKK50 pKa = 10.2WPPQRR55 pKa = 11.84NSKK58 pKa = 9.87LARR61 pKa = 11.84EE62 pKa = 4.33LLRR65 pKa = 11.84YY66 pKa = 6.36WCRR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.94PVGDD75 pKa = 3.37TDD77 pKa = 4.91GVGKK81 pKa = 10.44SLFQIDD87 pKa = 3.8HH88 pKa = 6.66SSLEE92 pKa = 4.08KK93 pKa = 9.85QVRR96 pKa = 11.84RR97 pKa = 11.84SSAGGVEE104 pKa = 4.34RR105 pKa = 11.84EE106 pKa = 4.12

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3626

0

3626

1077767

44

2528

297.2

32.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.602 ± 0.049

0.74 ± 0.014

8.84 ± 0.055

9.275 ± 0.058

3.197 ± 0.028

8.346 ± 0.036

2.032 ± 0.021

4.252 ± 0.033

1.58 ± 0.026

8.954 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.661 ± 0.02

2.11 ± 0.022

4.71 ± 0.028

2.455 ± 0.023

6.48 ± 0.044

5.435 ± 0.034

6.416 ± 0.033

9.175 ± 0.039

1.1 ± 0.017

2.641 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski