Tomato mosaic Trujillo virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato wrinkled mosaic virus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218MXV5|A0A218MXV5_9GEMI Nuclear shuttle protein OS=Tomato mosaic Trujillo virus OX=2015440 GN=NSP PE=3 SV=1
MM1 pKa = 8.27DD2 pKa = 4.15SQLVNPPHH10 pKa = 6.55AFNYY14 pKa = 9.71IEE16 pKa = 4.15SHH18 pKa = 6.24RR19 pKa = 11.84DD20 pKa = 3.22EE21 pKa = 4.7YY22 pKa = 11.24QLSHH26 pKa = 7.5DD27 pKa = 3.72LTEE30 pKa = 4.56IVLQFPSTASQLTARR45 pKa = 11.84LSRR48 pKa = 11.84SCMKK52 pKa = 9.84IDD54 pKa = 3.26HH55 pKa = 6.45CVIEE59 pKa = 4.31YY60 pKa = 9.79RR61 pKa = 11.84QQVPINATGSVIVEE75 pKa = 3.82IHH77 pKa = 7.1DD78 pKa = 4.31KK79 pKa = 11.26RR80 pKa = 11.84MTDD83 pKa = 3.41NEE85 pKa = 4.36SLQASWTFPIRR96 pKa = 11.84CNIDD100 pKa = 2.86LHH102 pKa = 6.12YY103 pKa = 10.57FSASFFSLKK112 pKa = 10.83DD113 pKa = 3.77PIPWKK118 pKa = 10.3LYY120 pKa = 10.64YY121 pKa = 9.61RR122 pKa = 11.84VCDD125 pKa = 3.8TNVHH129 pKa = 5.05QRR131 pKa = 11.84THH133 pKa = 5.37FAKK136 pKa = 10.85FKK138 pKa = 10.96GKK140 pKa = 10.52LKK142 pKa = 10.82LSTAKK147 pKa = 10.37HH148 pKa = 5.69SVDD151 pKa = 2.95IPFRR155 pKa = 11.84APTVKK160 pKa = 10.11ILSKK164 pKa = 10.79QFTDD168 pKa = 3.13KK169 pKa = 11.25DD170 pKa = 3.57VDD172 pKa = 4.22FSHH175 pKa = 7.29VDD177 pKa = 3.13YY178 pKa = 11.23GRR180 pKa = 11.84WEE182 pKa = 4.05RR183 pKa = 11.84KK184 pKa = 9.17PIRR187 pKa = 11.84CASMSRR193 pKa = 11.84VGLRR197 pKa = 11.84GPIEE201 pKa = 3.96IRR203 pKa = 11.84PGEE206 pKa = 4.2SWASRR211 pKa = 11.84STIGTGHH218 pKa = 6.19SEE220 pKa = 3.94ADD222 pKa = 3.34SEE224 pKa = 5.11VEE226 pKa = 4.55NEE228 pKa = 3.86LHH230 pKa = 7.24PYY232 pKa = 9.59RR233 pKa = 11.84HH234 pKa = 6.68LSRR237 pKa = 11.84LGTTVLDD244 pKa = 4.4PGEE247 pKa = 4.24SASVIGAQRR256 pKa = 11.84AEE258 pKa = 4.37SNITMSMGQLNEE270 pKa = 4.06LVRR273 pKa = 11.84TTVQEE278 pKa = 4.74CINSNCRR285 pKa = 11.84PSQPKK290 pKa = 10.09SLQQ293 pKa = 3.18

Molecular weight: 33.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218MXV7|A0A218MXV7_9GEMI AC4 OS=Tomato mosaic Trujillo virus OX=2015440 GN=AC4 PE=3 SV=1
MM1 pKa = 7.31QMGSLISMCSSNSKK15 pKa = 10.53GNSTARR21 pKa = 11.84IRR23 pKa = 11.84DD24 pKa = 3.6CSIWCPQPGQYY35 pKa = 10.15ISIRR39 pKa = 11.84TYY41 pKa = 10.98RR42 pKa = 11.84EE43 pKa = 3.84LNPAPTSSPTSRR55 pKa = 11.84KK56 pKa = 8.2TGTPPNGGCSRR67 pKa = 11.84STAEE71 pKa = 3.7VLEE74 pKa = 4.17AVNRR78 pKa = 11.84HH79 pKa = 5.83LTTQPPRR86 pKa = 11.84RR87 pKa = 3.83

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1512

87

365

216.0

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.026 ± 0.786

1.984 ± 0.305

5.093 ± 0.543

4.828 ± 0.769

3.77 ± 0.417

6.085 ± 0.533

3.571 ± 0.347

5.423 ± 0.387

5.82 ± 0.683

6.548 ± 0.643

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.489

5.556 ± 0.743

5.489 ± 0.508

4.233 ± 0.724

7.672 ± 0.925

9.193 ± 0.803

5.291 ± 0.541

6.415 ± 0.71

1.521 ± 0.106

4.101 ± 0.595
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski