Amino acid dipepetide frequency for Hibiscus syriacus (Rose of Sharon)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.08AlaAla: 6.08 ± 0.02	1.247AlaCys: 1.247 ± 0.007	3.153AlaAsp: 3.153 ± 0.01	4.108AlaGlu: 4.108 ± 0.013	2.71AlaPhe: 2.71 ± 0.011	3.924AlaGly: 3.924 ± 0.012	1.243AlaHis: 1.243 ± 0.006	3.728AlaIle: 3.728 ± 0.011	3.89AlaLys: 3.89 ± 0.013	6.412AlaLeu: 6.412 ± 0.016	1.874AlaMet: 1.874 ± 0.007	2.606AlaAsn: 2.606 ± 0.009	2.8AlaPro: 2.8 ± 0.011	2.011AlaGln: 2.011 ± 0.009	3.31AlaArg: 3.31 ± 0.012	5.951AlaSer: 5.951 ± 0.016	3.62AlaThr: 3.62 ± 0.01	4.725AlaVal: 4.725 ± 0.012	0.816AlaTrp: 0.816 ± 0.006	1.773AlaTyr: 1.773 ± 0.008	0.0AlaXaa: 0.0 ± 0.0
Cys	0.973CysAla: 0.973 ± 0.006	0.567CysCys: 0.567 ± 0.005	0.911CysAsp: 0.911 ± 0.006	0.905CysGlu: 0.905 ± 0.005	0.933CysPhe: 0.933 ± 0.005	1.387CysGly: 1.387 ± 0.007	0.497CysHis: 0.497 ± 0.004	1.009CysIle: 1.009 ± 0.005	1.199CysLys: 1.199 ± 0.008	1.915CysLeu: 1.915 ± 0.009	0.485CysMet: 0.485 ± 0.004	0.912CysAsn: 0.912 ± 0.005	0.966CysPro: 0.966 ± 0.006	0.611CysGln: 0.611 ± 0.004	1.137CysArg: 1.137 ± 0.005	1.933CysSer: 1.933 ± 0.01	0.865CysThr: 0.865 ± 0.005	1.033CysVal: 1.033 ± 0.006	0.291CysTrp: 0.291 ± 0.003	0.591CysTyr: 0.591 ± 0.004	0.0CysXaa: 0.0 ± 0.0
Asp	3.368AspAla: 3.368 ± 0.011	1.02AspCys: 1.02 ± 0.006	3.729AspAsp: 3.729 ± 0.014	4.074AspGlu: 4.074 ± 0.013	2.392AspPhe: 2.392 ± 0.009	3.873AspGly: 3.873 ± 0.012	1.257AspHis: 1.257 ± 0.007	2.973AspIle: 2.973 ± 0.01	2.709AspLys: 2.709 ± 0.01	4.976AspLeu: 4.976 ± 0.013	1.337AspMet: 1.337 ± 0.007	2.226AspAsn: 2.226 ± 0.008	2.599AspPro: 2.599 ± 0.009	1.736AspGln: 1.736 ± 0.008	2.472AspArg: 2.472 ± 0.01	4.333AspSer: 4.333 ± 0.014	2.16AspThr: 2.16 ± 0.009	3.665AspVal: 3.665 ± 0.011	0.735AspTrp: 0.735 ± 0.005	1.578AspTyr: 1.578 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.742GluAla: 4.742 ± 0.016	0.934GluCys: 0.934 ± 0.006	3.841GluAsp: 3.841 ± 0.013	6.025GluGlu: 6.025 ± 0.022	2.465GluPhe: 2.465 ± 0.009	3.618GluGly: 3.618 ± 0.01	1.274GluHis: 1.274 ± 0.007	3.709GluIle: 3.709 ± 0.012	4.635GluLys: 4.635 ± 0.017	5.925GluLeu: 5.925 ± 0.017	1.798GluMet: 1.798 ± 0.008	3.192GluAsn: 3.192 ± 0.012	2.171GluPro: 2.171 ± 0.009	2.159GluGln: 2.159 ± 0.009	3.311GluArg: 3.311 ± 0.012	4.593GluSer: 4.593 ± 0.016	3.22GluThr: 3.22 ± 0.012	4.054GluVal: 4.054 ± 0.012	0.753GluTrp: 0.753 ± 0.005	1.633GluTyr: 1.633 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.439PheAla: 2.439 ± 0.009	0.92PheCys: 0.92 ± 0.005	2.415PheAsp: 2.415 ± 0.009	2.366PheGlu: 2.366 ± 0.008	1.964PhePhe: 1.964 ± 0.01	3.08PheGly: 3.08 ± 0.01	1.159PheHis: 1.159 ± 0.007	2.181PheIle: 2.181 ± 0.01	2.226PheLys: 2.226 ± 0.009	4.123PheLeu: 4.123 ± 0.014	1.027PheMet: 1.027 ± 0.005	1.905PheAsn: 1.905 ± 0.008	2.127PhePro: 2.127 ± 0.009	1.575PheGln: 1.575 ± 0.008	2.124PheArg: 2.124 ± 0.009	4.108PheSer: 4.108 ± 0.013	2.082PheThr: 2.082 ± 0.009	2.744PheVal: 2.744 ± 0.011	0.603PheTrp: 0.603 ± 0.004	1.253PheTyr: 1.253 ± 0.006	0.0PheXaa: 0.0 ± 0.0
Gly	3.751GlyAla: 3.751 ± 0.014	1.359GlyCys: 1.359 ± 0.007	3.423GlyAsp: 3.423 ± 0.011	3.628GlyGlu: 3.628 ± 0.012	3.26GlyPhe: 3.26 ± 0.01	5.16GlyGly: 5.16 ± 0.021	1.575GlyHis: 1.575 ± 0.008	3.659GlyIle: 3.659 ± 0.011	4.164GlyLys: 4.164 ± 0.012	5.878GlyLeu: 5.878 ± 0.016	1.53GlyMet: 1.53 ± 0.007	3.306GlyAsn: 3.306 ± 0.013	2.351GlyPro: 2.351 ± 0.01	1.993GlyGln: 1.993 ± 0.009	3.689GlyArg: 3.689 ± 0.014	6.172GlySer: 6.172 ± 0.015	3.311GlyThr: 3.311 ± 0.012	4.242GlyVal: 4.242 ± 0.013	0.89GlyTrp: 0.89 ± 0.006	2.12GlyTyr: 2.12 ± 0.01	0.0GlyXaa: 0.0 ± 0.0
His	1.389HisAla: 1.389 ± 0.007	0.548HisCys: 0.548 ± 0.004	1.167HisAsp: 1.167 ± 0.006	1.28HisGlu: 1.28 ± 0.007	1.093HisPhe: 1.093 ± 0.006	1.786HisGly: 1.786 ± 0.008	0.941HisHis: 0.941 ± 0.007	1.201HisIle: 1.201 ± 0.006	1.181HisLys: 1.181 ± 0.006	2.411HisLeu: 2.411 ± 0.009	0.576HisMet: 0.576 ± 0.005	1.005HisAsn: 1.005 ± 0.006	1.398HisPro: 1.398 ± 0.006	1.044HisGln: 1.044 ± 0.006	1.462HisArg: 1.462 ± 0.007	1.929HisSer: 1.929 ± 0.01	0.934HisThr: 0.934 ± 0.005	1.58HisVal: 1.58 ± 0.006	0.329HisTrp: 0.329 ± 0.003	0.702HisTyr: 0.702 ± 0.004	0.0HisXaa: 0.0 ± 0.0
Ile	3.526IleAla: 3.526 ± 0.011	1.121IleCys: 1.121 ± 0.006	2.915IleAsp: 2.915 ± 0.01	3.231IleGlu: 3.231 ± 0.01	2.253IlePhe: 2.253 ± 0.01	3.451IleGly: 3.451 ± 0.012	1.309IleHis: 1.309 ± 0.006	2.727IleIle: 2.727 ± 0.01	3.002IleLys: 3.002 ± 0.011	5.003IleLeu: 5.003 ± 0.013	1.166IleMet: 1.166 ± 0.006	2.245IleAsn: 2.245 ± 0.009	2.933IlePro: 2.933 ± 0.011	1.952IleGln: 1.952 ± 0.008	2.738IleArg: 2.738 ± 0.011	4.818IleSer: 4.818 ± 0.013	2.598IleThr: 2.598 ± 0.01	3.484IleVal: 3.484 ± 0.013	0.755IleTrp: 0.755 ± 0.006	1.432IleTyr: 1.432 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	4.182LysAla: 4.182 ± 0.014	1.012LysCys: 1.012 ± 0.006	3.226LysAsp: 3.226 ± 0.012	4.634LysGlu: 4.634 ± 0.017	2.18LysPhe: 2.18 ± 0.009	3.791LysGly: 3.791 ± 0.013	1.428LysHis: 1.428 ± 0.007	3.242LysIle: 3.242 ± 0.01	4.75LysLys: 4.75 ± 0.018	6.031LysLeu: 6.031 ± 0.016	1.547LysMet: 1.547 ± 0.007	2.752LysAsn: 2.752 ± 0.01	2.892LysPro: 2.892 ± 0.012	2.396LysGln: 2.396 ± 0.009	3.644LysArg: 3.644 ± 0.013	4.717LysSer: 4.717 ± 0.014	3.017LysThr: 3.017 ± 0.01	3.876LysVal: 3.876 ± 0.013	0.861LysTrp: 0.861 ± 0.006	1.63LysTyr: 1.63 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	6.252LeuAla: 6.252 ± 0.015	1.822LeuCys: 1.822 ± 0.008	4.99LeuAsp: 4.99 ± 0.014	6.122LeuGlu: 6.122 ± 0.017	3.774LeuPhe: 3.774 ± 0.013	5.742LeuGly: 5.742 ± 0.014	2.563LeuHis: 2.563 ± 0.009	4.509LeuIle: 4.509 ± 0.012	6.273LeuLys: 6.273 ± 0.016	9.475LeuLeu: 9.475 ± 0.023	2.191LeuMet: 2.191 ± 0.009	4.134LeuAsn: 4.134 ± 0.011	5.045LeuPro: 5.045 ± 0.015	4.225LeuGln: 4.225 ± 0.013	5.325LeuArg: 5.325 ± 0.014	8.444LeuSer: 8.444 ± 0.022	4.41LeuThr: 4.41 ± 0.012	6.234LeuVal: 6.234 ± 0.016	1.238LeuTrp: 1.238 ± 0.006	2.446LeuTyr: 2.446 ± 0.009	0.0LeuXaa: 0.0 ± 0.0
Met	2.167MetAla: 2.167 ± 0.008	0.339MetCys: 0.339 ± 0.003	1.474MetAsp: 1.474 ± 0.007	2.057MetGlu: 2.057 ± 0.009	0.877MetPhe: 0.877 ± 0.006	1.65MetGly: 1.65 ± 0.008	0.558MetHis: 0.558 ± 0.004	1.238MetIle: 1.238 ± 0.007	1.727MetLys: 1.727 ± 0.008	2.225MetLeu: 2.225 ± 0.008	0.705MetMet: 0.705 ± 0.005	1.137MetAsn: 1.137 ± 0.005	1.077MetPro: 1.077 ± 0.006	0.974MetGln: 0.974 ± 0.006	1.216MetArg: 1.216 ± 0.006	1.812MetSer: 1.812 ± 0.007	1.089MetThr: 1.089 ± 0.006	1.75MetVal: 1.75 ± 0.008	0.262MetTrp: 0.262 ± 0.003	0.586MetTyr: 0.586 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.707AsnAla: 2.707 ± 0.01	0.879AsnCys: 0.879 ± 0.006	2.225AsnAsp: 2.225 ± 0.008	2.648AsnGlu: 2.648 ± 0.011	1.929AsnPhe: 1.929 ± 0.008	3.513AsnGly: 3.513 ± 0.012	1.186AsnHis: 1.186 ± 0.006	2.464AsnIle: 2.464 ± 0.009	2.557AsnLys: 2.557 ± 0.01	4.581AsnLeu: 4.581 ± 0.017	1.143AsnMet: 1.143 ± 0.006	2.416AsnAsn: 2.416 ± 0.011	2.429AsnPro: 2.429 ± 0.009	1.807AsnGln: 1.807 ± 0.008	2.236AsnArg: 2.236 ± 0.009	3.98AsnSer: 3.98 ± 0.015	2.025AsnThr: 2.025 ± 0.008	2.918AsnVal: 2.918 ± 0.01	0.601AsnTrp: 0.601 ± 0.004	1.313AsnTyr: 1.313 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	2.937ProAla: 2.937 ± 0.011	0.845ProCys: 0.845 ± 0.005	2.439ProAsp: 2.439 ± 0.009	3.062ProGlu: 3.062 ± 0.011	2.047ProPhe: 2.047 ± 0.009	2.745ProGly: 2.745 ± 0.011	1.067ProHis: 1.067 ± 0.006	2.37ProIle: 2.37 ± 0.009	2.825ProLys: 2.825 ± 0.011	4.316ProLeu: 4.316 ± 0.013	1.073ProMet: 1.073 ± 0.006	2.267ProAsn: 2.267 ± 0.01	3.867ProPro: 3.867 ± 0.033	1.775ProGln: 1.775 ± 0.01	2.502ProArg: 2.502 ± 0.01	5.142ProSer: 5.142 ± 0.016	2.746ProThr: 2.746 ± 0.009	3.163ProVal: 3.163 ± 0.011	0.646ProTrp: 0.646 ± 0.005	1.346ProTyr: 1.346 ± 0.008	0.0ProXaa: 0.0 ± 0.0
Gln	2.406GlnAla: 2.406 ± 0.009	0.596GlnCys: 0.596 ± 0.005	1.588GlnAsp: 1.588 ± 0.007	2.303GlnGlu: 2.303 ± 0.011	1.405GlnPhe: 1.405 ± 0.006	2.167GlnGly: 2.167 ± 0.01	0.974GlnHis: 0.974 ± 0.006	1.964GlnIle: 1.964 ± 0.008	2.259GlnLys: 2.259 ± 0.01	3.572GlnLeu: 3.572 ± 0.012	0.976GlnMet: 0.976 ± 0.006	1.758GlnAsn: 1.758 ± 0.008	1.797GlnPro: 1.797 ± 0.01	2.17GlnGln: 2.17 ± 0.018	2.205GlnArg: 2.205 ± 0.009	2.89GlnSer: 2.89 ± 0.011	1.736GlnThr: 1.736 ± 0.008	2.325GlnVal: 2.325 ± 0.009	0.508GlnTrp: 0.508 ± 0.004	0.914GlnTyr: 0.914 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.19ArgAla: 3.19 ± 0.011	1.095ArgCys: 1.095 ± 0.006	2.707ArgAsp: 2.707 ± 0.011	3.265ArgGlu: 3.265 ± 0.012	2.332ArgPhe: 2.332 ± 0.008	3.259ArgGly: 3.259 ± 0.012	1.323ArgHis: 1.323 ± 0.007	2.898ArgIle: 2.898 ± 0.009	3.899ArgLys: 3.899 ± 0.013	5.085ArgLeu: 5.085 ± 0.012	1.369ArgMet: 1.369 ± 0.006	2.584ArgAsn: 2.584 ± 0.009	2.384ArgPro: 2.384 ± 0.01	1.9ArgGln: 1.9 ± 0.008	3.982ArgArg: 3.982 ± 0.014	4.526ArgSer: 4.526 ± 0.013	2.526ArgThr: 2.526 ± 0.008	3.4ArgVal: 3.4 ± 0.011	0.784ArgTrp: 0.784 ± 0.005	1.477ArgTyr: 1.477 ± 0.006	0.0ArgXaa: 0.0 ± 0.0
Ser	5.187SerAla: 5.187 ± 0.012	1.769SerCys: 1.769 ± 0.008	4.514SerAsp: 4.514 ± 0.014	4.708SerGlu: 4.708 ± 0.014	4.117SerPhe: 4.117 ± 0.012	5.941SerGly: 5.941 ± 0.017	1.985SerHis: 1.985 ± 0.009	4.55SerIle: 4.55 ± 0.013	5.12SerLys: 5.12 ± 0.015	8.568SerLeu: 8.568 ± 0.023	2.219SerMet: 2.219 ± 0.009	4.218SerAsn: 4.218 ± 0.014	4.678SerPro: 4.678 ± 0.017	2.961SerGln: 2.961 ± 0.012	4.682SerArg: 4.682 ± 0.013	11.109SerSer: 11.109 ± 0.032	4.786SerThr: 4.786 ± 0.015	5.284SerVal: 5.284 ± 0.013	1.208SerTrp: 1.208 ± 0.007	2.299SerTyr: 2.299 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.465ThrAla: 3.465 ± 0.011	0.957ThrCys: 0.957 ± 0.005	2.433ThrAsp: 2.433 ± 0.009	2.819ThrGlu: 2.819 ± 0.009	2.033ThrPhe: 2.033 ± 0.009	3.337ThrGly: 3.337 ± 0.011	1.001ThrHis: 1.001 ± 0.006	2.721ThrIle: 2.721 ± 0.01	2.768ThrLys: 2.768 ± 0.009	4.584ThrLeu: 4.584 ± 0.013	1.229ThrMet: 1.229 ± 0.006	2.092ThrAsn: 2.092 ± 0.008	2.648ThrPro: 2.648 ± 0.011	1.54ThrGln: 1.54 ± 0.007	2.507ThrArg: 2.507 ± 0.009	4.629ThrSer: 4.629 ± 0.015	3.083ThrThr: 3.083 ± 0.011	3.476ThrVal: 3.476 ± 0.013	0.702ThrTrp: 0.702 ± 0.004	1.349ThrTyr: 1.349 ± 0.007	0.0ThrXaa: 0.0 ± 0.0
Val	4.755ValAla: 4.755 ± 0.013	1.184ValCys: 1.184 ± 0.006	3.809ValAsp: 3.809 ± 0.011	4.456ValGlu: 4.456 ± 0.014	2.798ValPhe: 2.798 ± 0.009	4.135ValGly: 4.135 ± 0.013	1.545ValHis: 1.545 ± 0.006	3.368ValIle: 3.368 ± 0.011	4.007ValLys: 4.007 ± 0.012	6.155ValLeu: 6.155 ± 0.016	1.549ValMet: 1.549 ± 0.007	2.746ValAsn: 2.746 ± 0.009	3.278ValPro: 3.278 ± 0.01	2.275ValGln: 2.275 ± 0.008	3.058ValArg: 3.058 ± 0.01	5.545ValSer: 5.545 ± 0.013	3.222ValThr: 3.222 ± 0.011	4.935ValVal: 4.935 ± 0.014	0.822ValTrp: 0.822 ± 0.005	1.926ValTyr: 1.926 ± 0.008	0.0ValXaa: 0.0 ± 0.0
Trp	0.748TrpAla: 0.748 ± 0.005	0.262TrpCys: 0.262 ± 0.003	0.731TrpAsp: 0.731 ± 0.004	0.81TrpGlu: 0.81 ± 0.005	0.573TrpPhe: 0.573 ± 0.005	0.787TrpGly: 0.787 ± 0.006	0.334TrpHis: 0.334 ± 0.003	0.765TrpIle: 0.765 ± 0.005	1.032TrpLys: 1.032 ± 0.006	1.262TrpLeu: 1.262 ± 0.006	0.367TrpMet: 0.367 ± 0.003	0.743TrpAsn: 0.743 ± 0.005	0.498TrpPro: 0.498 ± 0.004	0.472TrpGln: 0.472 ± 0.004	0.878TrpArg: 0.878 ± 0.005	1.044TrpSer: 1.044 ± 0.007	0.676TrpThr: 0.676 ± 0.005	0.876TrpVal: 0.876 ± 0.005	0.256TrpTrp: 0.256 ± 0.003	0.345TrpTyr: 0.345 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.716TyrAla: 1.716 ± 0.008	0.644TyrCys: 0.644 ± 0.004	1.522TyrAsp: 1.522 ± 0.007	1.552TyrGlu: 1.552 ± 0.007	1.286TyrPhe: 1.286 ± 0.007	2.117TyrGly: 2.117 ± 0.01	0.738TyrHis: 0.738 ± 0.005	1.384TyrIle: 1.384 ± 0.007	1.584TyrLys: 1.584 ± 0.01	2.719TyrLeu: 2.719 ± 0.009	0.761TyrMet: 0.761 ± 0.004	1.313TyrAsn: 1.313 ± 0.007	1.242TyrPro: 1.242 ± 0.007	0.937TyrGln: 0.937 ± 0.005	1.507TyrArg: 1.507 ± 0.007	2.227TyrSer: 2.227 ± 0.008	1.268TyrThr: 1.268 ± 0.006	1.803TyrVal: 1.803 ± 0.008	0.396TyrTrp: 0.396 ± 0.004	0.955TyrTyr: 0.955 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 82460 proteins (32943284 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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