Pseudomonas phage LKA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Stubburvirus; Pseudomonas virus LKA1

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0E607|Q0E607_9CAUD Uncharacterized protein OS=Pseudomonas phage LKA1 OX=386793 PE=4 SV=1
MM1 pKa = 7.68IYY3 pKa = 10.52LYY5 pKa = 10.68LFSLLWSLSAWALISFRR22 pKa = 11.84TDD24 pKa = 3.98EE25 pKa = 4.22KK26 pKa = 11.41LLWNLIDD33 pKa = 3.59SAVFAGVLTTMLAEE47 pKa = 4.14YY48 pKa = 9.62QWWVGLGRR56 pKa = 11.84NILEE60 pKa = 4.52YY61 pKa = 10.73INAA64 pKa = 4.27

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0E5Y7|Q0E5Y7_9CAUD DNA ligase OS=Pseudomonas phage LKA1 OX=386793 GN=gp27 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84KK3 pKa = 9.66SLMLQLGPTPKK14 pKa = 10.03PGVVLGIGWDD24 pKa = 3.43WSRR27 pKa = 11.84PWKK30 pKa = 8.57ATYY33 pKa = 10.35YY34 pKa = 9.62PLDD37 pKa = 3.64GGRR40 pKa = 11.84LVSGSLGVGKK50 pKa = 10.48VWVFYY55 pKa = 8.94WWRR58 pKa = 11.84KK59 pKa = 6.86

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13094

50

1003

229.7

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.547 ± 0.504

1.214 ± 0.173

5.72 ± 0.211

6.522 ± 0.361

3.04 ± 0.15

7.996 ± 0.288

2.123 ± 0.22

4.017 ± 0.181

4.162 ± 0.274

9.333 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.159

3.131 ± 0.172

4.468 ± 0.251

4.758 ± 0.333

6.85 ± 0.33

5.247 ± 0.324

5.667 ± 0.349

7.087 ± 0.308

1.657 ± 0.131

3.246 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski