Ovine adenovirus D serotype 7 (isolate OAV287) (OAdV-7) (Ovine adenovirus 7)
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83916|Q83916_ADEO7 RH6 OS=Ovine adenovirus D serotype 7 (isolate OAV287) OX=114430 PE=4 SV=2
MM1 pKa = 6.76 NQHH4 pKa = 6.12 GRR6 pKa = 11.84 MLYY9 pKa = 9.99 PNSEE13 pKa = 4.4 LLSFLSPLNRR23 pKa = 11.84 HH24 pKa = 5.63 QYY26 pKa = 9.25 QVYY29 pKa = 9.87 CIVSSRR35 pKa = 11.84 YY36 pKa = 9.2 LNYY39 pKa = 9.05 WARR42 pKa = 11.84 GPFRR46 pKa = 11.84 MAFRR50 pKa = 11.84 FNGDD54 pKa = 2.65 KK55 pKa = 10.17 TVYY58 pKa = 10.6 VLAQKK63 pKa = 10.81 DD64 pKa = 3.93 LFPEE68 pKa = 4.54 FDD70 pKa = 3.48 ALDD73 pKa = 3.73 YY74 pKa = 11.11 LKK76 pKa = 10.2 WANFEE81 pKa = 4.17 NMGTGFFEE89 pKa = 4.33 VDD91 pKa = 2.59 WDD93 pKa = 4.01 NLVGVLNDD101 pKa = 3.77 RR102 pKa = 11.84 EE103 pKa = 4.29 SLIAEE108 pKa = 4.36 VVFVTSNVQDD118 pKa = 3.6 EE119 pKa = 4.53 TWNFFRR125 pKa = 11.84 YY126 pKa = 8.86 YY127 pKa = 8.55 WEE129 pKa = 4.74 NYY131 pKa = 8.95 FSSFPIKK138 pKa = 10.24 PDD140 pKa = 2.99 WQQ142 pKa = 3.81
Molecular weight: 17.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.737
IPC2_protein 4.952
IPC_protein 4.8
Toseland 4.673
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.66
Grimsley 4.596
Solomon 4.762
Lehninger 4.724
Nozaki 4.889
DTASelect 5.054
Thurlkill 4.685
EMBOSS 4.673
Sillero 4.94
Patrickios 3.3
IPC_peptide 4.774
IPC2_peptide 4.927
IPC2.peptide.svr19 4.942
Protein with the highest isoelectric point:
>tr|Q7T9P4|Q7T9P4_ADEO7 22K OS=Ovine adenovirus D serotype 7 (isolate OAV287) OX=114430 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 8.75 VTNNTALAGGAYY14 pKa = 9.93 RR15 pKa = 11.84 KK16 pKa = 9.57 RR17 pKa = 11.84 KK18 pKa = 9.9 KK19 pKa = 10.23 KK20 pKa = 9.29 FQRR23 pKa = 11.84 PKK25 pKa = 7.92 PRR27 pKa = 11.84 KK28 pKa = 8.88 RR29 pKa = 11.84 ARR31 pKa = 11.84 KK32 pKa = 8.75 SKK34 pKa = 10.51 KK35 pKa = 9.13 PPKK38 pKa = 10.13 SEE40 pKa = 3.51 NTYY43 pKa = 8.29 VQRR46 pKa = 11.84 FWKK49 pKa = 10.31 NPPRR53 pKa = 11.84 NYY55 pKa = 9.81 ILKK58 pKa = 9.99 EE59 pKa = 3.74 PTKK62 pKa = 10.03 PISSYY67 pKa = 11.27 ARR69 pKa = 11.84 WIPPEE74 pKa = 3.91 IVDD77 pKa = 4.21 VNNITVNIPNTITASRR93 pKa = 11.84 LPKK96 pKa = 10.24 TEE98 pKa = 3.98 FQEE101 pKa = 4.36 TEE103 pKa = 4.05 VFKK106 pKa = 10.73 DD107 pKa = 5.89 ARR109 pKa = 11.84 DD110 pKa = 3.12 QWYY113 pKa = 9.19 FPIRR117 pKa = 11.84 PSDD120 pKa = 3.79 GEE122 pKa = 3.99 HH123 pKa = 6.31 DD124 pKa = 3.54 TDD126 pKa = 4.54 VKK128 pKa = 11.11 VKK130 pKa = 10.55 KK131 pKa = 9.76 KK132 pKa = 9.75 WSLDD136 pKa = 3.26 TVLQFLQSSPKK147 pKa = 10.15 HH148 pKa = 5.35 IRR150 pKa = 11.84 QLLLTSLFGSLLGLILDD167 pKa = 4.33 TLFGGPWNLTSRR179 pKa = 11.84 LLRR182 pKa = 11.84 LIISVVPGGRR192 pKa = 11.84 ILLTALDD199 pKa = 3.82 GLGYY203 pKa = 10.68 FLGNSANPIHH213 pKa = 6.9 LIEE216 pKa = 4.95 NPMMQAFGNSIQKK229 pKa = 10.23 QISPRR234 pKa = 11.84 LAEE237 pKa = 5.27 DD238 pKa = 3.68 IIKK241 pKa = 10.68 AADD244 pKa = 3.61 EE245 pKa = 4.24 QIGGGFMRR253 pKa = 11.84 TIASILSAAASAGTHH268 pKa = 5.0 FTMALPAIPIAAVRR282 pKa = 11.84 PFMRR286 pKa = 5.27
Molecular weight: 32.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.235
IPC2_protein 9.648
IPC_protein 9.897
Toseland 10.599
ProMoST 10.218
Dawson 10.701
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 11.169
Grimsley 10.73
Solomon 10.745
Lehninger 10.73
Nozaki 10.555
DTASelect 10.321
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.877
IPC_peptide 10.76
IPC2_peptide 8.814
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30
0
30
9187
54
1071
306.2
35.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.887 ± 0.31
2.416 ± 0.453
4.419 ± 0.198
5.508 ± 0.469
5.758 ± 0.271
4.637 ± 0.318
1.948 ± 0.189
6.466 ± 0.332
6.727 ± 0.699
10.167 ± 0.639
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.025 ± 0.19
6.814 ± 0.418
5.192 ± 0.307
4.365 ± 0.309
4.387 ± 0.472
7.75 ± 0.343
5.78 ± 0.416
5.072 ± 0.292
1.557 ± 0.176
4.125 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here