Zalophus californianus papillomavirus 1
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2X1C4|F2X1C4_9PAPI Replication protein E1 OS=Zalophus californianus papillomavirus 1 OX=998829 GN=E1 PE=3 SV=1
MM1 pKa = 7.34 IGKK4 pKa = 8.5 EE5 pKa = 3.95 ATLKK9 pKa = 10.97 DD10 pKa = 3.43 IVLEE14 pKa = 4.13 EE15 pKa = 4.22 QPTVADD21 pKa = 4.33 EE22 pKa = 4.32 LWCDD26 pKa = 3.46 EE27 pKa = 4.24 EE28 pKa = 4.96 LPPEE32 pKa = 4.08 EE33 pKa = 5.06 AEE35 pKa = 3.97 PDD37 pKa = 2.88 RR38 pKa = 11.84 RR39 pKa = 11.84 APYY42 pKa = 9.45 KK43 pKa = 8.84 VHH45 pKa = 7.31 AYY47 pKa = 9.98 CGYY50 pKa = 9.24 CKK52 pKa = 10.23 RR53 pKa = 11.84 GVRR56 pKa = 11.84 LVIYY60 pKa = 10.14 SDD62 pKa = 3.34 TDD64 pKa = 3.71 SVQKK68 pKa = 9.92 LHH70 pKa = 7.09 RR71 pKa = 11.84 LLLGPFDD78 pKa = 4.24 IVCPPCAVRR87 pKa = 11.84 CRR89 pKa = 11.84 YY90 pKa = 10.4 NNGSS94 pKa = 3.25
Molecular weight: 10.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.907
IPC2_protein 5.143
IPC_protein 4.991
Toseland 4.902
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.105
Wikipedia 4.838
Rodwell 4.876
Grimsley 4.813
Solomon 4.965
Lehninger 4.914
Nozaki 5.08
DTASelect 5.219
Thurlkill 4.914
EMBOSS 4.863
Sillero 5.156
Patrickios 3.325
IPC_peptide 4.965
IPC2_peptide 5.143
IPC2.peptide.svr19 5.17
Protein with the highest isoelectric point:
>tr|F2X1C7|F2X1C7_9PAPI Minor capsid protein L2 OS=Zalophus californianus papillomavirus 1 OX=998829 GN=L2 PE=3 SV=1
MM1 pKa = 6.08 VTSYY5 pKa = 11.47 SPLWTLSPALHH16 pKa = 6.75 RR17 pKa = 11.84 PPTAAEE23 pKa = 3.97 RR24 pKa = 11.84 LRR26 pKa = 11.84 GLLEE30 pKa = 4.25 GLPPTDD36 pKa = 4.01 LPYY39 pKa = 10.73 PPGAWGPLPTTHH51 pKa = 7.15 LPGPSIYY58 pKa = 10.48 DD59 pKa = 3.32 QEE61 pKa = 4.58 QPLPPPQPLPRR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 PTLRR80 pKa = 11.84 EE81 pKa = 3.4 QRR83 pKa = 11.84 QALNDD88 pKa = 3.6 TLKK91 pKa = 10.67 QVEE94 pKa = 4.12 KK95 pKa = 10.78 DD96 pKa = 2.91 AWTLRR101 pKa = 11.84 TDD103 pKa = 3.9 ILGDD107 pKa = 3.58 LDD109 pKa = 4.31 NFFDD113 pKa = 4.32 KK114 pKa = 11.18 LGIVPRR120 pKa = 11.84 QQ121 pKa = 3.07
Molecular weight: 13.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.829
IPC2_protein 8.697
IPC_protein 9.033
Toseland 9.297
ProMoST 9.121
Dawson 9.575
Bjellqvist 9.297
Wikipedia 9.794
Rodwell 9.648
Grimsley 9.677
Solomon 9.75
Lehninger 9.721
Nozaki 9.18
DTASelect 9.311
Thurlkill 9.37
EMBOSS 9.692
Sillero 9.472
Patrickios 5.308
IPC_peptide 9.736
IPC2_peptide 8.039
IPC2.peptide.svr19 7.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2412
94
629
344.6
38.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.126 ± 0.908
1.99 ± 0.672
5.887 ± 0.469
5.846 ± 0.499
3.275 ± 0.486
8.416 ± 0.658
2.322 ± 0.315
3.151 ± 0.355
3.317 ± 0.61
8.748 ± 0.694
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.41 ± 0.384
2.861 ± 0.471
8.043 ± 1.172
4.478 ± 0.707
7.96 ± 0.599
6.509 ± 0.893
6.219 ± 0.425
6.177 ± 1.346
1.783 ± 0.422
3.483 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here