Lachnospiraceae bacterium XBB2008

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5AQJ6|A0A1G5AQJ6_9FIRM Diguanylate cyclase (GGDEF) domain-containing protein OS=Lachnospiraceae bacterium XBB2008 OX=1520826 GN=SAMN02910292_00078 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84DD3 pKa = 2.9KK4 pKa = 10.99RR5 pKa = 11.84ITVLILTALCTVMLCGCGDD24 pKa = 5.12EE25 pKa = 4.97IPEE28 pKa = 4.29LTPSQQEE35 pKa = 4.16MITEE39 pKa = 4.0YY40 pKa = 10.92SAALLLKK47 pKa = 9.89YY48 pKa = 10.56DD49 pKa = 3.8SNTPSRR55 pKa = 11.84LLPEE59 pKa = 4.42GSVVSIAYY67 pKa = 9.67VDD69 pKa = 4.05PNDD72 pKa = 3.9PNAVLVEE79 pKa = 4.33TTPEE83 pKa = 4.02TEE85 pKa = 4.19TTSDD89 pKa = 3.07IAVDD93 pKa = 3.71NVPDD97 pKa = 3.71VTSEE101 pKa = 4.09DD102 pKa = 3.62VSVTDD107 pKa = 3.28VTTGQTTGGQHH118 pKa = 6.77AGFSNFLADD127 pKa = 4.3LGVSFDD133 pKa = 3.57YY134 pKa = 11.18SGVYY138 pKa = 9.69EE139 pKa = 4.5VVDD142 pKa = 4.44SYY144 pKa = 12.09PEE146 pKa = 4.38GNDD149 pKa = 2.96VNPYY153 pKa = 8.28FTVDD157 pKa = 3.01ATDD160 pKa = 3.6GNKK163 pKa = 10.42LLVIHH168 pKa = 6.87FNLTNNSASDD178 pKa = 3.35MDD180 pKa = 4.45VNLNTLNLRR189 pKa = 11.84YY190 pKa = 9.33RR191 pKa = 11.84VSLNGGKK198 pKa = 9.8NKK200 pKa = 9.8FVMTTLLEE208 pKa = 4.09NDD210 pKa = 2.91ILSYY214 pKa = 11.12VGTLGSGQTEE224 pKa = 4.79DD225 pKa = 3.24IVAICEE231 pKa = 3.63ISEE234 pKa = 4.25AEE236 pKa = 4.09GANIQTIDD244 pKa = 3.42FTVRR248 pKa = 11.84GDD250 pKa = 3.64GFSDD254 pKa = 3.8VMSLQQ259 pKa = 3.25

Molecular weight: 27.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5EH67|A0A1G5EH67_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium XBB2008 OX=1520826 GN=SAMN02910292_01236 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.93KK9 pKa = 7.56RR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.03VHH16 pKa = 6.53GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.72VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84HH40 pKa = 5.42KK41 pKa = 10.58LTVV44 pKa = 3.06

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3048

0

3048

1097907

40

4505

360.2

40.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.569 ± 0.046

1.408 ± 0.017

7.021 ± 0.039

7.334 ± 0.046

4.065 ± 0.031

7.044 ± 0.041

1.77 ± 0.018

7.683 ± 0.041

5.782 ± 0.044

8.526 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.124 ± 0.021

4.202 ± 0.034

3.41 ± 0.032

2.452 ± 0.021

4.764 ± 0.034

6.27 ± 0.032

5.457 ± 0.039

6.756 ± 0.035

0.921 ± 0.015

4.441 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski