Amino acid dipepetide frequency for Pseudidiomarina aestuarii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.879AlaAla: 10.879 ± 0.3
0.867AlaCys: 0.867 ± 0.056
6.064AlaAsp: 6.064 ± 0.172
7.132AlaGlu: 7.132 ± 0.198
3.539AlaPhe: 3.539 ± 0.131
7.052AlaGly: 7.052 ± 0.163
1.779AlaHis: 1.779 ± 0.074
6.013AlaIle: 6.013 ± 0.152
3.867AlaLys: 3.867 ± 0.13
11.017AlaLeu: 11.017 ± 0.227
2.693AlaMet: 2.693 ± 0.101
3.324AlaAsn: 3.324 ± 0.118
3.506AlaPro: 3.506 ± 0.117
4.971AlaGln: 4.971 ± 0.158
5.059AlaArg: 5.059 ± 0.14
5.285AlaSer: 5.285 ± 0.135
5.332AlaThr: 5.332 ± 0.147
7.103AlaVal: 7.103 ± 0.168
1.148AlaTrp: 1.148 ± 0.069
2.354AlaTyr: 2.354 ± 0.083
0.0AlaXaa: 0.0 ± 0.0
Cys
0.907CysAla: 0.907 ± 0.059
0.171CysCys: 0.171 ± 0.022
0.55CysAsp: 0.55 ± 0.048
0.419CysGlu: 0.419 ± 0.038
0.357CysPhe: 0.357 ± 0.036
0.769CysGly: 0.769 ± 0.056
0.222CysHis: 0.222 ± 0.029
0.426CysIle: 0.426 ± 0.039
0.284CysLys: 0.284 ± 0.03
0.751CysLeu: 0.751 ± 0.054
0.182CysMet: 0.182 ± 0.024
0.244CysAsn: 0.244 ± 0.028
0.339CysPro: 0.339 ± 0.036
0.339CysGln: 0.339 ± 0.038
0.492CysArg: 0.492 ± 0.042
0.561CysSer: 0.561 ± 0.043
0.386CysThr: 0.386 ± 0.036
0.605CysVal: 0.605 ± 0.045
0.179CysTrp: 0.179 ± 0.028
0.179CysTyr: 0.179 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.142AspAla: 5.142 ± 0.134
0.481AspCys: 0.481 ± 0.044
3.218AspAsp: 3.218 ± 0.111
4.031AspGlu: 4.031 ± 0.139
2.657AspPhe: 2.657 ± 0.106
4.126AspGly: 4.126 ± 0.156
1.203AspHis: 1.203 ± 0.06
3.572AspIle: 3.572 ± 0.117
2.249AspLys: 2.249 ± 0.106
5.441AspLeu: 5.441 ± 0.14
1.502AspMet: 1.502 ± 0.079
1.924AspAsn: 1.924 ± 0.086
2.343AspPro: 2.343 ± 0.093
2.329AspGln: 2.329 ± 0.104
2.868AspArg: 2.868 ± 0.11
3.222AspSer: 3.222 ± 0.111
2.733AspThr: 2.733 ± 0.108
4.304AspVal: 4.304 ± 0.122
0.977AspTrp: 0.977 ± 0.069
2.252AspTyr: 2.252 ± 0.094
0.0AspXaa: 0.0 ± 0.0
Glu
6.21GluAla: 6.21 ± 0.186
0.379GluCys: 0.379 ± 0.032
2.759GluAsp: 2.759 ± 0.087
3.207GluGlu: 3.207 ± 0.134
2.588GluPhe: 2.588 ± 0.097
3.597GluGly: 3.597 ± 0.146
1.83GluHis: 1.83 ± 0.076
3.404GluIle: 3.404 ± 0.134
2.646GluLys: 2.646 ± 0.114
7.705GluLeu: 7.705 ± 0.191
1.516GluMet: 1.516 ± 0.083
2.103GluAsn: 2.103 ± 0.096
2.763GluPro: 2.763 ± 0.192
4.475GluGln: 4.475 ± 0.148
4.895GluArg: 4.895 ± 0.132
3.604GluSer: 3.604 ± 0.131
3.331GluThr: 3.331 ± 0.127
4.548GluVal: 4.548 ± 0.157
0.871GluTrp: 0.871 ± 0.067
1.556GluTyr: 1.556 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
4.133PheAla: 4.133 ± 0.122
0.43PheCys: 0.43 ± 0.037
2.744PheAsp: 2.744 ± 0.105
2.467PheGlu: 2.467 ± 0.1
1.553PhePhe: 1.553 ± 0.078
3.495PheGly: 3.495 ± 0.136
0.776PheHis: 0.776 ± 0.054
2.54PheIle: 2.54 ± 0.109
1.465PheLys: 1.465 ± 0.077
3.378PheLeu: 3.378 ± 0.128
0.882PheMet: 0.882 ± 0.058
1.706PheAsn: 1.706 ± 0.086
1.458PhePro: 1.458 ± 0.081
1.374PheGln: 1.374 ± 0.065
1.881PheArg: 1.881 ± 0.086
2.912PheSer: 2.912 ± 0.117
2.212PheThr: 2.212 ± 0.091
2.846PheVal: 2.846 ± 0.122
0.532PheTrp: 0.532 ± 0.041
1.188PheTyr: 1.188 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
5.747GlyAla: 5.747 ± 0.163
0.74GlyCys: 0.74 ± 0.06
4.129GlyAsp: 4.129 ± 0.141
4.556GlyGlu: 4.556 ± 0.138
3.222GlyPhe: 3.222 ± 0.134
5.113GlyGly: 5.113 ± 0.195
1.596GlyHis: 1.596 ± 0.077
4.337GlyIle: 4.337 ± 0.139
3.127GlyLys: 3.127 ± 0.15
7.296GlyLeu: 7.296 ± 0.172
2.001GlyMet: 2.001 ± 0.097
2.38GlyAsn: 2.38 ± 0.101
2.015GlyPro: 2.015 ± 0.091
3.069GlyGln: 3.069 ± 0.106
3.677GlyArg: 3.677 ± 0.137
4.355GlySer: 4.355 ± 0.151
3.655GlyThr: 3.655 ± 0.137
5.266GlyVal: 5.266 ± 0.158
1.053GlyTrp: 1.053 ± 0.067
2.544GlyTyr: 2.544 ± 0.097
0.0GlyXaa: 0.0 ± 0.0
His
1.906HisAla: 1.906 ± 0.09
0.292HisCys: 0.292 ± 0.035
1.108HisAsp: 1.108 ± 0.066
1.374HisGlu: 1.374 ± 0.072
1.013HisPhe: 1.013 ± 0.068
1.837HisGly: 1.837 ± 0.087
0.627HisHis: 0.627 ± 0.05
1.272HisIle: 1.272 ± 0.064
0.831HisLys: 0.831 ± 0.048
1.997HisLeu: 1.997 ± 0.09
0.364HisMet: 0.364 ± 0.039
0.656HisAsn: 0.656 ± 0.047
1.188HisPro: 1.188 ± 0.076
1.232HisGln: 1.232 ± 0.075
1.203HisArg: 1.203 ± 0.057
1.232HisSer: 1.232 ± 0.064
0.9HisThr: 0.9 ± 0.054
1.195HisVal: 1.195 ± 0.064
0.372HisTrp: 0.372 ± 0.037
0.86HisTyr: 0.86 ± 0.051
0.0HisXaa: 0.0 ± 0.0
Ile
6.669IleAla: 6.669 ± 0.154
0.554IleCys: 0.554 ± 0.044
4.009IleAsp: 4.009 ± 0.114
4.399IleGlu: 4.399 ± 0.139
2.11IlePhe: 2.11 ± 0.099
4.61IleGly: 4.61 ± 0.159
1.148IleHis: 1.148 ± 0.061
3.163IleIle: 3.163 ± 0.131
2.099IleLys: 2.099 ± 0.092
5.011IleLeu: 5.011 ± 0.15
1.133IleMet: 1.133 ± 0.069
2.041IleAsn: 2.041 ± 0.08
2.85IlePro: 2.85 ± 0.104
2.373IleGln: 2.373 ± 0.086
3.255IleArg: 3.255 ± 0.1
3.393IleSer: 3.393 ± 0.112
3.021IleThr: 3.021 ± 0.101
4.188IleVal: 4.188 ± 0.151
0.569IleTrp: 0.569 ± 0.05
1.418IleTyr: 1.418 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
3.747LysAla: 3.747 ± 0.163
0.204LysCys: 0.204 ± 0.047
1.921LysAsp: 1.921 ± 0.093
2.252LysGlu: 2.252 ± 0.115
1.348LysPhe: 1.348 ± 0.067
2.365LysGly: 2.365 ± 0.108
0.969LysHis: 0.969 ± 0.067
2.012LysIle: 2.012 ± 0.087
1.706LysLys: 1.706 ± 0.101
4.257LysLeu: 4.257 ± 0.143
0.991LysMet: 0.991 ± 0.063
1.312LysAsn: 1.312 ± 0.066
2.063LysPro: 2.063 ± 0.085
2.161LysGln: 2.161 ± 0.096
2.522LysArg: 2.522 ± 0.106
2.431LysSer: 2.431 ± 0.104
1.99LysThr: 1.99 ± 0.084
2.941LysVal: 2.941 ± 0.104
0.441LysTrp: 0.441 ± 0.039
1.05LysTyr: 1.05 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
11.735LeuAla: 11.735 ± 0.227
0.959LeuCys: 0.959 ± 0.066
5.955LeuAsp: 5.955 ± 0.162
6.659LeuGlu: 6.659 ± 0.177
3.907LeuPhe: 3.907 ± 0.127
6.874LeuGly: 6.874 ± 0.197
2.194LeuHis: 2.194 ± 0.105
5.802LeuIle: 5.802 ± 0.173
4.191LeuLys: 4.191 ± 0.144
11.309LeuLeu: 11.309 ± 0.252
2.311LeuMet: 2.311 ± 0.099
3.83LeuAsn: 3.83 ± 0.117
4.975LeuPro: 4.975 ± 0.121
5.113LeuGln: 5.113 ± 0.174
6.05LeuArg: 6.05 ± 0.178
6.782LeuSer: 6.782 ± 0.163
6.309LeuThr: 6.309 ± 0.159
7.311LeuVal: 7.311 ± 0.162
1.265LeuTrp: 1.265 ± 0.068
2.58LeuTyr: 2.58 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
2.511MetAla: 2.511 ± 0.112
0.12MetCys: 0.12 ± 0.021
1.093MetAsp: 1.093 ± 0.071
1.184MetGlu: 1.184 ± 0.073
0.714MetPhe: 0.714 ± 0.052
1.567MetGly: 1.567 ± 0.092
0.463MetHis: 0.463 ± 0.041
1.294MetIle: 1.294 ± 0.079
1.119MetLys: 1.119 ± 0.072
2.613MetLeu: 2.613 ± 0.101
0.722MetMet: 0.722 ± 0.057
1.028MetAsn: 1.028 ± 0.071
1.17MetPro: 1.17 ± 0.065
1.17MetGln: 1.17 ± 0.059
1.476MetArg: 1.476 ± 0.069
1.684MetSer: 1.684 ± 0.081
1.604MetThr: 1.604 ± 0.07
1.64MetVal: 1.64 ± 0.079
0.219MetTrp: 0.219 ± 0.029
0.55MetTyr: 0.55 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.122
0.302AsnCys: 0.302 ± 0.033
2.278AsnAsp: 2.278 ± 0.104
2.114AsnGlu: 2.114 ± 0.097
1.389AsnPhe: 1.389 ± 0.087
2.558AsnGly: 2.558 ± 0.112
0.652AsnHis: 0.652 ± 0.049
1.95AsnIle: 1.95 ± 0.091
1.367AsnLys: 1.367 ± 0.088
3.415AsnLeu: 3.415 ± 0.121
0.846AsnMet: 0.846 ± 0.054
1.403AsnAsn: 1.403 ± 0.089
1.822AsnPro: 1.822 ± 0.089
1.687AsnGln: 1.687 ± 0.087
1.964AsnArg: 1.964 ± 0.09
2.096AsnSer: 2.096 ± 0.088
1.837AsnThr: 1.837 ± 0.097
2.369AsnVal: 2.369 ± 0.096
0.616AsnTrp: 0.616 ± 0.047
1.166AsnTyr: 1.166 ± 0.074
0.0AsnXaa: 0.0 ± 0.0
Pro
4.198ProAla: 4.198 ± 0.133
0.284ProCys: 0.284 ± 0.033
2.46ProAsp: 2.46 ± 0.102
3.652ProGlu: 3.652 ± 0.2
1.753ProPhe: 1.753 ± 0.082
2.679ProGly: 2.679 ± 0.108
0.849ProHis: 0.849 ± 0.056
2.453ProIle: 2.453 ± 0.09
1.647ProLys: 1.647 ± 0.078
4.581ProLeu: 4.581 ± 0.146
0.893ProMet: 0.893 ± 0.059
1.662ProAsn: 1.662 ± 0.085
1.37ProPro: 1.37 ± 0.078
2.245ProGln: 2.245 ± 0.103
1.87ProArg: 1.87 ± 0.084
2.394ProSer: 2.394 ± 0.09
2.424ProThr: 2.424 ± 0.102
3.185ProVal: 3.185 ± 0.102
0.561ProTrp: 0.561 ± 0.045
1.319ProTyr: 1.319 ± 0.075
0.0ProXaa: 0.0 ± 0.0
Gln
5.077GlnAla: 5.077 ± 0.167
0.313GlnCys: 0.313 ± 0.038
1.986GlnAsp: 1.986 ± 0.078
2.599GlnGlu: 2.599 ± 0.108
1.917GlnPhe: 1.917 ± 0.085
3.043GlnGly: 3.043 ± 0.1
1.305GlnHis: 1.305 ± 0.072
2.322GlnIle: 2.322 ± 0.095
1.633GlnLys: 1.633 ± 0.083
6.487GlnLeu: 6.487 ± 0.186
1.064GlnMet: 1.064 ± 0.061
1.341GlnAsn: 1.341 ± 0.076
2.478GlnPro: 2.478 ± 0.108
4.158GlnGln: 4.158 ± 0.188
3.666GlnArg: 3.666 ± 0.144
2.883GlnSer: 2.883 ± 0.111
2.362GlnThr: 2.362 ± 0.088
3.747GlnVal: 3.747 ± 0.132
0.816GlnTrp: 0.816 ± 0.062
1.239GlnTyr: 1.239 ± 0.071
0.0GlnXaa: 0.0 ± 0.0
Arg
4.993ArgAla: 4.993 ± 0.147
0.346ArgCys: 0.346 ± 0.035
3.24ArgAsp: 3.24 ± 0.108
4.107ArgGlu: 4.107 ± 0.116
2.496ArgPhe: 2.496 ± 0.093
3.433ArgGly: 3.433 ± 0.114
1.359ArgHis: 1.359 ± 0.073
3.604ArgIle: 3.604 ± 0.123
2.267ArgLys: 2.267 ± 0.108
6.017ArgLeu: 6.017 ± 0.164
1.483ArgMet: 1.483 ± 0.077
2.015ArgAsn: 2.015 ± 0.086
2.085ArgPro: 2.085 ± 0.098
3.018ArgGln: 3.018 ± 0.119
3.353ArgArg: 3.353 ± 0.131
3.149ArgSer: 3.149 ± 0.112
2.566ArgThr: 2.566 ± 0.091
4.169ArgVal: 4.169 ± 0.121
0.966ArgTrp: 0.966 ± 0.06
2.114ArgTyr: 2.114 ± 0.095
0.0ArgXaa: 0.0 ± 0.0
Ser
5.962SerAla: 5.962 ± 0.157
0.518SerCys: 0.518 ± 0.043
3.375SerAsp: 3.375 ± 0.113
3.575SerGlu: 3.575 ± 0.123
2.624SerPhe: 2.624 ± 0.086
4.953SerGly: 4.953 ± 0.152
1.214SerHis: 1.214 ± 0.06
3.692SerIle: 3.692 ± 0.137
2.362SerLys: 2.362 ± 0.107
6.13SerLeu: 6.13 ± 0.16
1.48SerMet: 1.48 ± 0.063
2.201SerAsn: 2.201 ± 0.1
2.438SerPro: 2.438 ± 0.093
2.548SerGln: 2.548 ± 0.111
3.109SerArg: 3.109 ± 0.119
3.87SerSer: 3.87 ± 0.146
3.349SerThr: 3.349 ± 0.125
4.118SerVal: 4.118 ± 0.122
0.762SerTrp: 0.762 ± 0.05
1.848SerTyr: 1.848 ± 0.09
0.0SerXaa: 0.0 ± 0.0
Thr
5.39ThrAla: 5.39 ± 0.154
0.394ThrCys: 0.394 ± 0.044
3.018ThrAsp: 3.018 ± 0.115
3.302ThrGlu: 3.302 ± 0.117
1.997ThrPhe: 1.997 ± 0.098
4.122ThrGly: 4.122 ± 0.147
1.002ThrHis: 1.002 ± 0.06
3.327ThrIle: 3.327 ± 0.135
1.695ThrLys: 1.695 ± 0.085
6.137ThrLeu: 6.137 ± 0.146
1.177ThrMet: 1.177 ± 0.068
1.757ThrAsn: 1.757 ± 0.091
2.675ThrPro: 2.675 ± 0.101
2.46ThrGln: 2.46 ± 0.102
2.569ThrArg: 2.569 ± 0.101
3.138ThrSer: 3.138 ± 0.113
2.989ThrThr: 2.989 ± 0.125
4.352ThrVal: 4.352 ± 0.134
0.718ThrTrp: 0.718 ± 0.061
1.523ThrTyr: 1.523 ± 0.081
0.0ThrXaa: 0.0 ± 0.0
Val
7.034ValAla: 7.034 ± 0.169
0.649ValCys: 0.649 ± 0.051
4.465ValAsp: 4.465 ± 0.14
4.636ValGlu: 4.636 ± 0.147
2.62ValPhe: 2.62 ± 0.11
4.906ValGly: 4.906 ± 0.158
1.235ValHis: 1.235 ± 0.059
4.796ValIle: 4.796 ± 0.141
2.865ValLys: 2.865 ± 0.128
7.267ValLeu: 7.267 ± 0.197
1.793ValMet: 1.793 ± 0.079
2.708ValAsn: 2.708 ± 0.091
3.054ValPro: 3.054 ± 0.121
2.996ValGln: 2.996 ± 0.114
3.834ValArg: 3.834 ± 0.138
4.578ValSer: 4.578 ± 0.143
4.687ValThr: 4.687 ± 0.141
5.707ValVal: 5.707 ± 0.172
0.813ValTrp: 0.813 ± 0.063
1.972ValTyr: 1.972 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.907TrpAla: 0.907 ± 0.051
0.149TrpCys: 0.149 ± 0.022
0.631TrpAsp: 0.631 ± 0.041
0.623TrpGlu: 0.623 ± 0.049
0.765TrpPhe: 0.765 ± 0.056
0.663TrpGly: 0.663 ± 0.048
0.324TrpHis: 0.324 ± 0.042
0.641TrpIle: 0.641 ± 0.053
0.343TrpLys: 0.343 ± 0.035
2.103TrpLeu: 2.103 ± 0.11
0.354TrpMet: 0.354 ± 0.038
0.408TrpAsn: 0.408 ± 0.04
0.496TrpPro: 0.496 ± 0.049
1.093TrpGln: 1.093 ± 0.074
1.006TrpArg: 1.006 ± 0.065
0.802TrpSer: 0.802 ± 0.046
0.609TrpThr: 0.609 ± 0.047
0.922TrpVal: 0.922 ± 0.056
0.23TrpTrp: 0.23 ± 0.031
0.536TrpTyr: 0.536 ± 0.054
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.66TyrAla: 2.66 ± 0.1
0.211TyrCys: 0.211 ± 0.029
1.571TyrAsp: 1.571 ± 0.076
1.698TyrGlu: 1.698 ± 0.092
1.403TyrPhe: 1.403 ± 0.076
2.117TyrGly: 2.117 ± 0.096
0.711TyrHis: 0.711 ± 0.045
1.392TyrIle: 1.392 ± 0.069
0.922TyrLys: 0.922 ± 0.051
3.196TyrLeu: 3.196 ± 0.123
0.55TyrMet: 0.55 ± 0.048
0.962TyrAsn: 0.962 ± 0.069
1.392TyrPro: 1.392 ± 0.068
1.625TyrGln: 1.625 ± 0.088
2.034TyrArg: 2.034 ± 0.092
1.76TyrSer: 1.76 ± 0.077
1.447TyrThr: 1.447 ± 0.07
2.096TyrVal: 2.096 ± 0.087
0.499TyrTrp: 0.499 ± 0.044
0.991TyrTyr: 0.991 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 947 proteins (274386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski