Amino acid dipepetide frequency for Microbacterium phage Musetta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.839AlaAla: 12.839 ± 1.427
0.707AlaCys: 0.707 ± 0.18
6.474AlaAsp: 6.474 ± 0.58
7.997AlaGlu: 7.997 ± 0.878
3.101AlaPhe: 3.101 ± 0.367
7.399AlaGly: 7.399 ± 0.892
1.578AlaHis: 1.578 ± 0.298
5.603AlaIle: 5.603 ± 0.541
4.787AlaLys: 4.787 ± 0.582
9.629AlaLeu: 9.629 ± 0.778
2.72AlaMet: 2.72 ± 0.406
4.678AlaAsn: 4.678 ± 0.527
3.754AlaPro: 3.754 ± 0.431
4.134AlaGln: 4.134 ± 0.55
6.963AlaArg: 6.963 ± 0.534
5.712AlaSer: 5.712 ± 1.107
5.875AlaThr: 5.875 ± 0.65
7.072AlaVal: 7.072 ± 0.707
1.795AlaTrp: 1.795 ± 0.291
2.666AlaTyr: 2.666 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.49CysAla: 0.49 ± 0.167
0.0CysCys: 0.0 ± 0.0
0.49CysAsp: 0.49 ± 0.198
0.435CysGlu: 0.435 ± 0.191
0.272CysPhe: 0.272 ± 0.121
0.707CysGly: 0.707 ± 0.233
0.163CysHis: 0.163 ± 0.097
0.109CysIle: 0.109 ± 0.079
0.326CysLys: 0.326 ± 0.14
0.326CysLeu: 0.326 ± 0.123
0.054CysMet: 0.054 ± 0.055
0.054CysAsn: 0.054 ± 0.055
0.109CysPro: 0.109 ± 0.114
0.054CysGln: 0.054 ± 0.052
0.49CysArg: 0.49 ± 0.163
0.218CysSer: 0.218 ± 0.104
0.109CysThr: 0.109 ± 0.071
0.109CysVal: 0.109 ± 0.069
0.109CysTrp: 0.109 ± 0.086
0.163CysTyr: 0.163 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
5.767AspAla: 5.767 ± 0.718
0.544AspCys: 0.544 ± 0.175
4.57AspAsp: 4.57 ± 0.558
5.168AspGlu: 5.168 ± 0.591
2.013AspPhe: 2.013 ± 0.375
6.256AspGly: 6.256 ± 0.612
1.088AspHis: 1.088 ± 0.26
3.699AspIle: 3.699 ± 0.429
3.373AspLys: 3.373 ± 0.48
4.95AspLeu: 4.95 ± 0.665
1.795AspMet: 1.795 ± 0.318
2.122AspAsn: 2.122 ± 0.332
3.318AspPro: 3.318 ± 0.513
1.741AspGln: 1.741 ± 0.396
3.21AspArg: 3.21 ± 0.445
3.754AspSer: 3.754 ± 0.501
3.754AspThr: 3.754 ± 0.547
4.243AspVal: 4.243 ± 0.555
1.251AspTrp: 1.251 ± 0.258
2.176AspTyr: 2.176 ± 0.352
0.0AspXaa: 0.0 ± 0.0
Glu
7.725GluAla: 7.725 ± 0.849
0.544GluCys: 0.544 ± 0.185
4.352GluAsp: 4.352 ± 0.506
4.134GluGlu: 4.134 ± 0.53
2.72GluPhe: 2.72 ± 0.307
5.223GluGly: 5.223 ± 0.526
1.523GluHis: 1.523 ± 0.352
4.57GluIle: 4.57 ± 0.458
3.754GluLys: 3.754 ± 0.475
5.277GluLeu: 5.277 ± 0.545
2.067GluMet: 2.067 ± 0.388
2.176GluAsn: 2.176 ± 0.385
2.666GluPro: 2.666 ± 0.454
2.502GluGln: 2.502 ± 0.3
4.95GluArg: 4.95 ± 0.609
4.896GluSer: 4.896 ± 0.589
2.611GluThr: 2.611 ± 0.373
7.072GluVal: 7.072 ± 0.538
1.197GluTrp: 1.197 ± 0.234
2.774GluTyr: 2.774 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
3.645PheAla: 3.645 ± 0.519
0.109PheCys: 0.109 ± 0.084
2.448PheAsp: 2.448 ± 0.47
2.23PheGlu: 2.23 ± 0.336
1.088PhePhe: 1.088 ± 0.293
2.557PheGly: 2.557 ± 0.316
0.544PheHis: 0.544 ± 0.187
1.741PheIle: 1.741 ± 0.295
1.197PheLys: 1.197 ± 0.264
1.85PheLeu: 1.85 ± 0.303
0.762PheMet: 0.762 ± 0.203
1.36PheAsn: 1.36 ± 0.236
1.523PhePro: 1.523 ± 0.31
1.36PheGln: 1.36 ± 0.255
2.285PheArg: 2.285 ± 0.342
2.339PheSer: 2.339 ± 0.363
2.122PheThr: 2.122 ± 0.385
1.958PheVal: 1.958 ± 0.419
0.435PheTrp: 0.435 ± 0.124
1.469PheTyr: 1.469 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
7.181GlyAla: 7.181 ± 0.857
0.435GlyCys: 0.435 ± 0.183
5.114GlyAsp: 5.114 ± 0.507
7.127GlyGlu: 7.127 ± 0.642
3.862GlyPhe: 3.862 ± 0.533
7.018GlyGly: 7.018 ± 0.881
1.142GlyHis: 1.142 ± 0.249
4.08GlyIle: 4.08 ± 0.41
4.842GlyLys: 4.842 ± 0.493
6.311GlyLeu: 6.311 ± 0.986
1.741GlyMet: 1.741 ± 0.313
2.938GlyAsn: 2.938 ± 0.509
2.285GlyPro: 2.285 ± 0.351
3.155GlyGln: 3.155 ± 0.37
6.093GlyArg: 6.093 ± 0.484
4.515GlySer: 4.515 ± 0.522
5.495GlyThr: 5.495 ± 0.562
6.311GlyVal: 6.311 ± 0.489
1.251GlyTrp: 1.251 ± 0.26
3.21GlyTyr: 3.21 ± 0.45
0.0GlyXaa: 0.0 ± 0.0
His
2.285HisAla: 2.285 ± 0.373
0.054HisCys: 0.054 ± 0.057
1.197HisAsp: 1.197 ± 0.288
1.414HisGlu: 1.414 ± 0.311
0.598HisPhe: 0.598 ± 0.177
1.36HisGly: 1.36 ± 0.276
0.272HisHis: 0.272 ± 0.138
0.816HisIle: 0.816 ± 0.203
0.762HisLys: 0.762 ± 0.186
1.523HisLeu: 1.523 ± 0.313
0.653HisMet: 0.653 ± 0.218
0.544HisAsn: 0.544 ± 0.197
1.034HisPro: 1.034 ± 0.263
0.326HisGln: 0.326 ± 0.135
1.034HisArg: 1.034 ± 0.225
0.653HisSer: 0.653 ± 0.183
1.306HisThr: 1.306 ± 0.364
1.36HisVal: 1.36 ± 0.261
0.272HisTrp: 0.272 ± 0.116
0.707HisTyr: 0.707 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.95IleAla: 4.95 ± 0.533
0.109IleCys: 0.109 ± 0.08
3.536IleAsp: 3.536 ± 0.41
3.971IleGlu: 3.971 ± 0.535
1.686IlePhe: 1.686 ± 0.285
4.57IleGly: 4.57 ± 0.412
1.251IleHis: 1.251 ± 0.303
2.067IleIle: 2.067 ± 0.356
2.23IleLys: 2.23 ± 0.346
3.21IleLeu: 3.21 ± 0.428
0.979IleMet: 0.979 ± 0.228
2.448IleAsn: 2.448 ± 0.392
2.067IlePro: 2.067 ± 0.262
2.176IleGln: 2.176 ± 0.284
4.134IleArg: 4.134 ± 0.413
3.046IleSer: 3.046 ± 0.408
3.264IleThr: 3.264 ± 0.406
4.08IleVal: 4.08 ± 0.469
0.762IleTrp: 0.762 ± 0.202
1.414IleTyr: 1.414 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
5.549LysAla: 5.549 ± 0.554
0.218LysCys: 0.218 ± 0.099
2.067LysAsp: 2.067 ± 0.299
3.046LysGlu: 3.046 ± 0.533
1.85LysPhe: 1.85 ± 0.301
3.482LysGly: 3.482 ± 0.422
0.816LysHis: 0.816 ± 0.224
2.666LysIle: 2.666 ± 0.389
3.046LysLys: 3.046 ± 0.541
3.59LysLeu: 3.59 ± 0.492
1.578LysMet: 1.578 ± 0.327
1.251LysAsn: 1.251 ± 0.342
2.122LysPro: 2.122 ± 0.34
0.762LysGln: 0.762 ± 0.196
3.101LysArg: 3.101 ± 0.427
2.285LysSer: 2.285 ± 0.455
2.938LysThr: 2.938 ± 0.496
3.59LysVal: 3.59 ± 0.531
0.762LysTrp: 0.762 ± 0.243
1.197LysTyr: 1.197 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
8.595LeuAla: 8.595 ± 1.035
0.272LeuCys: 0.272 ± 0.111
5.821LeuAsp: 5.821 ± 0.545
6.528LeuGlu: 6.528 ± 0.623
1.632LeuPhe: 1.632 ± 0.294
5.875LeuGly: 5.875 ± 0.902
0.979LeuHis: 0.979 ± 0.247
3.373LeuIle: 3.373 ± 0.469
2.611LeuLys: 2.611 ± 0.457
3.264LeuLeu: 3.264 ± 0.477
1.523LeuMet: 1.523 ± 0.289
3.21LeuAsn: 3.21 ± 0.562
3.427LeuPro: 3.427 ± 0.384
2.448LeuGln: 2.448 ± 0.384
4.461LeuArg: 4.461 ± 0.432
4.842LeuSer: 4.842 ± 0.471
4.733LeuThr: 4.733 ± 0.448
4.842LeuVal: 4.842 ± 0.525
1.469LeuTrp: 1.469 ± 0.28
2.176LeuTyr: 2.176 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
3.101MetAla: 3.101 ± 0.42
0.109MetCys: 0.109 ± 0.07
0.979MetAsp: 0.979 ± 0.258
1.414MetGlu: 1.414 ± 0.349
0.49MetPhe: 0.49 ± 0.168
2.013MetGly: 2.013 ± 0.289
0.707MetHis: 0.707 ± 0.2
0.762MetIle: 0.762 ± 0.304
1.306MetLys: 1.306 ± 0.246
1.523MetLeu: 1.523 ± 0.313
0.49MetMet: 0.49 ± 0.17
1.034MetAsn: 1.034 ± 0.206
0.707MetPro: 0.707 ± 0.183
0.925MetGln: 0.925 ± 0.218
1.686MetArg: 1.686 ± 0.384
2.122MetSer: 2.122 ± 0.331
2.176MetThr: 2.176 ± 0.263
0.87MetVal: 0.87 ± 0.237
0.326MetTrp: 0.326 ± 0.117
0.707MetTyr: 0.707 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
3.59AsnAla: 3.59 ± 0.489
0.054AsnCys: 0.054 ± 0.056
1.85AsnAsp: 1.85 ± 0.362
2.122AsnGlu: 2.122 ± 0.344
1.088AsnPhe: 1.088 ± 0.244
4.406AsnGly: 4.406 ± 0.499
0.979AsnHis: 0.979 ± 0.212
1.469AsnIle: 1.469 ± 0.258
1.251AsnLys: 1.251 ± 0.275
3.536AsnLeu: 3.536 ± 0.434
0.925AsnMet: 0.925 ± 0.183
1.632AsnAsn: 1.632 ± 0.322
2.339AsnPro: 2.339 ± 0.354
0.925AsnGln: 0.925 ± 0.221
2.448AsnArg: 2.448 ± 0.441
2.23AsnSer: 2.23 ± 0.389
2.285AsnThr: 2.285 ± 0.395
2.72AsnVal: 2.72 ± 0.353
0.762AsnTrp: 0.762 ± 0.19
1.414AsnTyr: 1.414 ± 0.272
0.0AsnXaa: 0.0 ± 0.0
Pro
4.026ProAla: 4.026 ± 0.49
0.109ProCys: 0.109 ± 0.079
2.992ProAsp: 2.992 ± 0.438
3.264ProGlu: 3.264 ± 0.382
1.523ProPhe: 1.523 ± 0.244
4.787ProGly: 4.787 ± 0.547
0.87ProHis: 0.87 ± 0.265
2.013ProIle: 2.013 ± 0.405
2.394ProLys: 2.394 ± 0.323
2.829ProLeu: 2.829 ± 0.417
1.034ProMet: 1.034 ± 0.274
1.904ProAsn: 1.904 ± 0.323
1.741ProPro: 1.741 ± 0.357
1.469ProGln: 1.469 ± 0.305
2.666ProArg: 2.666 ± 0.471
2.502ProSer: 2.502 ± 0.4
3.373ProThr: 3.373 ± 0.439
3.264ProVal: 3.264 ± 0.598
0.762ProTrp: 0.762 ± 0.182
0.87ProTyr: 0.87 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
3.427GlnAla: 3.427 ± 0.412
0.109GlnCys: 0.109 ± 0.076
2.122GlnAsp: 2.122 ± 0.417
2.72GlnGlu: 2.72 ± 0.419
1.142GlnPhe: 1.142 ± 0.197
2.394GlnGly: 2.394 ± 0.448
0.653GlnHis: 0.653 ± 0.182
2.557GlnIle: 2.557 ± 0.489
1.197GlnLys: 1.197 ± 0.226
1.904GlnLeu: 1.904 ± 0.305
0.762GlnMet: 0.762 ± 0.243
1.36GlnAsn: 1.36 ± 0.25
0.979GlnPro: 0.979 ± 0.23
0.87GlnGln: 0.87 ± 0.218
1.958GlnArg: 1.958 ± 0.337
1.469GlnSer: 1.469 ± 0.268
1.85GlnThr: 1.85 ± 0.282
2.829GlnVal: 2.829 ± 0.464
0.544GlnTrp: 0.544 ± 0.161
0.979GlnTyr: 0.979 ± 0.235
0.0GlnXaa: 0.0 ± 0.0
Arg
6.311ArgAla: 6.311 ± 0.58
0.163ArgCys: 0.163 ± 0.084
3.862ArgAsp: 3.862 ± 0.516
4.842ArgGlu: 4.842 ± 0.483
2.122ArgPhe: 2.122 ± 0.34
4.896ArgGly: 4.896 ± 0.429
0.979ArgHis: 0.979 ± 0.177
3.808ArgIle: 3.808 ± 0.486
3.862ArgLys: 3.862 ± 0.557
5.386ArgLeu: 5.386 ± 0.546
1.686ArgMet: 1.686 ± 0.301
2.285ArgAsn: 2.285 ± 0.41
2.883ArgPro: 2.883 ± 0.479
1.741ArgGln: 1.741 ± 0.363
4.243ArgArg: 4.243 ± 0.663
3.645ArgSer: 3.645 ± 0.524
3.754ArgThr: 3.754 ± 0.379
4.787ArgVal: 4.787 ± 0.533
1.85ArgTrp: 1.85 ± 0.356
2.394ArgTyr: 2.394 ± 0.43
0.0ArgXaa: 0.0 ± 0.0
Ser
6.365SerAla: 6.365 ± 0.671
0.163SerCys: 0.163 ± 0.085
3.536SerAsp: 3.536 ± 0.432
3.046SerGlu: 3.046 ± 0.411
2.122SerPhe: 2.122 ± 0.339
5.495SerGly: 5.495 ± 0.686
0.925SerHis: 0.925 ± 0.241
3.754SerIle: 3.754 ± 0.402
2.883SerLys: 2.883 ± 0.42
4.08SerLeu: 4.08 ± 0.481
1.251SerMet: 1.251 ± 0.224
1.958SerAsn: 1.958 ± 0.409
2.448SerPro: 2.448 ± 0.374
1.686SerGln: 1.686 ± 0.359
3.264SerArg: 3.264 ± 0.349
4.57SerSer: 4.57 ± 0.652
3.754SerThr: 3.754 ± 0.43
4.134SerVal: 4.134 ± 0.57
1.469SerTrp: 1.469 ± 0.313
1.958SerTyr: 1.958 ± 0.312
0.0SerXaa: 0.0 ± 0.0
Thr
6.256ThrAla: 6.256 ± 0.518
0.653ThrCys: 0.653 ± 0.217
4.243ThrAsp: 4.243 ± 0.416
4.352ThrGlu: 4.352 ± 0.462
2.774ThrPhe: 2.774 ± 0.395
5.658ThrGly: 5.658 ± 0.612
1.251ThrHis: 1.251 ± 0.239
2.611ThrIle: 2.611 ± 0.385
1.904ThrLys: 1.904 ± 0.407
4.515ThrLeu: 4.515 ± 0.476
1.142ThrMet: 1.142 ± 0.284
1.904ThrAsn: 1.904 ± 0.364
4.298ThrPro: 4.298 ± 0.564
1.632ThrGln: 1.632 ± 0.344
3.427ThrArg: 3.427 ± 0.442
2.448ThrSer: 2.448 ± 0.305
4.08ThrThr: 4.08 ± 0.531
5.495ThrVal: 5.495 ± 0.618
1.306ThrTrp: 1.306 ± 0.243
1.85ThrTyr: 1.85 ± 0.352
0.0ThrXaa: 0.0 ± 0.0
Val
7.616ValAla: 7.616 ± 0.735
0.272ValCys: 0.272 ± 0.132
5.331ValAsp: 5.331 ± 0.549
5.658ValGlu: 5.658 ± 0.542
1.795ValPhe: 1.795 ± 0.329
6.093ValGly: 6.093 ± 0.612
1.523ValHis: 1.523 ± 0.363
3.699ValIle: 3.699 ± 0.607
2.611ValLys: 2.611 ± 0.434
4.624ValLeu: 4.624 ± 0.547
1.197ValMet: 1.197 ± 0.268
3.155ValAsn: 3.155 ± 0.45
3.971ValPro: 3.971 ± 0.526
2.176ValGln: 2.176 ± 0.357
5.93ValArg: 5.93 ± 0.667
4.57ValSer: 4.57 ± 0.399
5.495ValThr: 5.495 ± 0.505
4.787ValVal: 4.787 ± 0.452
0.979ValTrp: 0.979 ± 0.216
1.904ValTyr: 1.904 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
1.469TrpAla: 1.469 ± 0.283
0.054TrpCys: 0.054 ± 0.055
1.251TrpAsp: 1.251 ± 0.223
1.36TrpGlu: 1.36 ± 0.283
0.435TrpPhe: 0.435 ± 0.167
1.414TrpGly: 1.414 ± 0.303
0.49TrpHis: 0.49 ± 0.139
0.87TrpIle: 0.87 ± 0.213
0.326TrpLys: 0.326 ± 0.143
1.741TrpLeu: 1.741 ± 0.294
0.435TrpMet: 0.435 ± 0.151
0.979TrpAsn: 0.979 ± 0.231
0.925TrpPro: 0.925 ± 0.277
0.544TrpGln: 0.544 ± 0.141
1.414TrpArg: 1.414 ± 0.281
1.142TrpSer: 1.142 ± 0.289
0.925TrpThr: 0.925 ± 0.213
1.197TrpVal: 1.197 ± 0.241
0.381TrpTrp: 0.381 ± 0.139
0.762TrpTyr: 0.762 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.298TyrAla: 4.298 ± 0.407
0.109TyrCys: 0.109 ± 0.074
2.557TyrAsp: 2.557 ± 0.394
1.904TyrGlu: 1.904 ± 0.277
0.707TyrPhe: 0.707 ± 0.178
2.502TyrGly: 2.502 ± 0.402
0.435TyrHis: 0.435 ± 0.143
1.686TyrIle: 1.686 ± 0.256
1.251TyrLys: 1.251 ± 0.258
2.013TyrLeu: 2.013 ± 0.36
0.598TyrMet: 0.598 ± 0.257
1.034TyrAsn: 1.034 ± 0.216
1.795TyrPro: 1.795 ± 0.272
1.197TyrGln: 1.197 ± 0.233
1.741TyrArg: 1.741 ± 0.399
1.904TyrSer: 1.904 ± 0.332
1.904TyrThr: 1.904 ± 0.37
2.666TyrVal: 2.666 ± 0.305
0.49TyrTrp: 0.49 ± 0.143
0.816TyrTyr: 0.816 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski