Escherichia phage IMM-002

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus IMM002

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384WIN6|A0A384WIN6_9CAUD 50S ribosomal protein L31 OS=Escherichia phage IMM-002 OX=2041760 PE=4 SV=1
MM1 pKa = 7.34LVSSNGTTSEE11 pKa = 4.05SGQDD15 pKa = 3.46FTSEE19 pKa = 4.22DD20 pKa = 3.54LQEE23 pKa = 3.97QCEE26 pKa = 4.05LVGIGIAFTLVLTNFLTRR44 pKa = 11.84YY45 pKa = 7.14VCHH48 pKa = 6.5MIILLFEE55 pKa = 4.22

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384WIK9|A0A384WIK9_9CAUD Uncharacterized protein OS=Escherichia phage IMM-002 OX=2041760 PE=4 SV=1
MM1 pKa = 7.54AVTMRR6 pKa = 11.84LSSGTVFPTLRR17 pKa = 11.84IPSPLSKK24 pKa = 10.54VRR26 pKa = 11.84VRR28 pKa = 11.84FSRR31 pKa = 11.84PAHH34 pKa = 5.09WLMRR38 pKa = 11.84LMWRR42 pKa = 11.84TPGCKK47 pKa = 8.3GTQWVTQSLVPLRR60 pKa = 11.84LRR62 pKa = 11.84AQPQLVLLKK71 pKa = 10.84AHH73 pKa = 6.46LQVIANRR80 pKa = 11.84RR81 pKa = 11.84TNGG84 pKa = 3.06

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14173

25

1296

170.8

18.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.573 ± 0.357

1.305 ± 0.227

5.743 ± 0.281

6.103 ± 0.319

3.556 ± 0.177

7.938 ± 0.443

1.785 ± 0.189

4.72 ± 0.158

5.687 ± 0.34

8.664 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.163

4.163 ± 0.287

4.17 ± 0.225

4.092 ± 0.355

5.877 ± 0.262

6.978 ± 0.35

5.976 ± 0.287

7.359 ± 0.317

1.453 ± 0.157

3.168 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski