Capybara microvirus Cap3_SP_612
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W689|A0A4P8W689_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_612 OX=2585482 PE=4 SV=1
MM1 pKa = 7.73 GILGVFRR8 pKa = 11.84 TSSKK12 pKa = 10.86 SRR14 pKa = 11.84 IGEE17 pKa = 3.77 INYY20 pKa = 9.34 APSMTEE26 pKa = 3.78 DD27 pKa = 3.07 AHH29 pKa = 6.93 LLSIEE34 pKa = 4.41 QIVKK38 pKa = 10.22 DD39 pKa = 3.64 YY40 pKa = 11.33 SRR42 pKa = 11.84 GILHH46 pKa = 7.18 PEE48 pKa = 4.01 FNHH51 pKa = 6.4 SAVYY55 pKa = 10.59 DD56 pKa = 4.12 DD57 pKa = 5.5 GEE59 pKa = 4.54 EE60 pKa = 3.85 ISNVQNYY67 pKa = 10.44 DD68 pKa = 3.48 DD69 pKa = 4.59 LTDD72 pKa = 4.41 LFPNGVNSIYY82 pKa = 10.32 HH83 pKa = 5.39 LQDD86 pKa = 3.57 RR87 pKa = 11.84 EE88 pKa = 4.18 PSSEE92 pKa = 4.0 PQKK95 pKa = 10.84 MNDD98 pKa = 3.38 SAPEE102 pKa = 3.93 SEE104 pKa = 4.67 TNIEE108 pKa = 4.38 TKK110 pKa = 10.91 NEE112 pKa = 3.78
Molecular weight: 12.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.388
IPC2_protein 4.406
IPC_protein 4.304
Toseland 4.151
ProMoST 4.368
Dawson 4.266
Bjellqvist 4.457
Wikipedia 4.139
Rodwell 4.151
Grimsley 4.062
Solomon 4.253
Lehninger 4.215
Nozaki 4.38
DTASelect 4.52
Thurlkill 4.164
EMBOSS 4.164
Sillero 4.418
Patrickios 3.541
IPC_peptide 4.266
IPC2_peptide 4.418
IPC2.peptide.svr19 4.328
Protein with the highest isoelectric point:
>tr|A0A4P8W6G3|A0A4P8W6G3_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_612 OX=2585482 PE=3 SV=1
MM1 pKa = 7.78 DD2 pKa = 6.31 CIRR5 pKa = 11.84 PFVKK9 pKa = 10.06 TIGHH13 pKa = 6.48 GLDD16 pKa = 3.35 ANYY19 pKa = 10.13 IPLPCGRR26 pKa = 11.84 CLACRR31 pKa = 11.84 VNYY34 pKa = 7.83 TRR36 pKa = 11.84 QWTQRR41 pKa = 11.84 LLAEE45 pKa = 4.87 SYY47 pKa = 10.23 HH48 pKa = 6.33 SKK50 pKa = 10.41 NAYY53 pKa = 9.97 FLTLTYY59 pKa = 10.62 EE60 pKa = 4.41 DD61 pKa = 3.82 SCLPRR66 pKa = 11.84 DD67 pKa = 4.7 DD68 pKa = 5.2 NDD70 pKa = 3.6 NPCVCKK76 pKa = 10.3 RR77 pKa = 11.84 DD78 pKa = 3.12 IQLFIKK84 pKa = 10.28 RR85 pKa = 11.84 LRR87 pKa = 11.84 KK88 pKa = 9.56 HH89 pKa = 6.31 YY90 pKa = 9.24 PDD92 pKa = 3.41 SNIRR96 pKa = 11.84 YY97 pKa = 8.58 FLGSEE102 pKa = 4.15 YY103 pKa = 11.03 GEE105 pKa = 4.09 EE106 pKa = 4.12 TLRR109 pKa = 11.84 PHH111 pKa = 5.56 YY112 pKa = 10.23 HH113 pKa = 6.81 AIFYY117 pKa = 9.19 NLPDD121 pKa = 3.61 EE122 pKa = 6.35 LIFQPQSAGGDD133 pKa = 3.01 QWHH136 pKa = 5.97 YY137 pKa = 10.72 HH138 pKa = 6.91 DD139 pKa = 5.6 KK140 pKa = 9.44 SQHH143 pKa = 5.11 MINRR147 pKa = 11.84 KK148 pKa = 9.49 INDD151 pKa = 3.18 YY152 pKa = 7.27 WQKK155 pKa = 10.92 GFIEE159 pKa = 4.29 IDD161 pKa = 3.69 PINRR165 pKa = 11.84 QRR167 pKa = 11.84 AEE169 pKa = 3.79 YY170 pKa = 8.22 CAKK173 pKa = 10.31 YY174 pKa = 10.01 FVFRR178 pKa = 11.84 KK179 pKa = 9.97 DD180 pKa = 3.22 VEE182 pKa = 4.45 QILVPNFNLMSRR194 pKa = 11.84 KK195 pKa = 9.47 PGIGYY200 pKa = 8.72 QRR202 pKa = 11.84 IDD204 pKa = 3.76 EE205 pKa = 4.6 IKK207 pKa = 10.97 DD208 pKa = 2.99 KK209 pKa = 11.03 VRR211 pKa = 11.84 YY212 pKa = 9.27 YY213 pKa = 11.0 NLNGMLTDD221 pKa = 3.49 KK222 pKa = 10.31 GTYY225 pKa = 9.21 CAIPRR230 pKa = 11.84 YY231 pKa = 9.3 YY232 pKa = 10.27 KK233 pKa = 10.7 DD234 pKa = 3.26 KK235 pKa = 10.59 IYY237 pKa = 10.94 SKK239 pKa = 9.86 EE240 pKa = 3.82 EE241 pKa = 3.68 RR242 pKa = 11.84 EE243 pKa = 4.11 EE244 pKa = 3.94 LFNRR248 pKa = 11.84 YY249 pKa = 9.67 LNMRR253 pKa = 11.84 CTLSIEE259 pKa = 4.13 EE260 pKa = 4.53 IEE262 pKa = 4.51 FNRR265 pKa = 11.84 QKK267 pKa = 11.08 LQGDD271 pKa = 3.92 EE272 pKa = 4.69 LEE274 pKa = 4.48 EE275 pKa = 3.95 SLIRR279 pKa = 11.84 NMTFKK284 pKa = 10.84 NKK286 pKa = 9.06 KK287 pKa = 9.44 RR288 pKa = 11.84 KK289 pKa = 9.18 LL290 pKa = 3.57
Molecular weight: 34.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.829
IPC2_protein 8.009
IPC_protein 7.849
Toseland 7.527
ProMoST 8.39
Dawson 8.565
Bjellqvist 8.843
Wikipedia 8.521
Rodwell 8.595
Grimsley 7.527
Solomon 8.639
Lehninger 8.639
Nozaki 8.96
DTASelect 8.595
Thurlkill 8.639
EMBOSS 8.741
Sillero 8.946
Patrickios 4.024
IPC_peptide 8.639
IPC2_peptide 7.805
IPC2.peptide.svr19 8.02
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1318
80
539
263.6
29.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.297 ± 1.151
1.29 ± 0.587
4.856 ± 0.614
7.056 ± 0.536
4.401 ± 0.772
6.222 ± 0.678
2.124 ± 0.363
6.753 ± 0.317
5.311 ± 0.737
7.891 ± 0.467
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.352 ± 0.152
7.891 ± 0.554
5.008 ± 0.461
4.021 ± 0.314
5.008 ± 0.943
6.601 ± 1.029
6.07 ± 1.123
4.552 ± 0.717
0.835 ± 0.116
5.463 ± 0.64
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here