Coregonus sp. balchen
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 41641 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F9CRJ2|A0A6F9CRJ2_9TELE RING-type domain-containing protein OS=Coregonus sp. 'balchen' OX=861768 GN=CSTEINMANNI_LOCUS4562867 PE=4 SV=1
MM1 pKa = 7.97 EE2 pKa = 5.72 DD3 pKa = 3.01 NCRR6 pKa = 11.84 LVPNKK11 pKa = 10.09 DD12 pKa = 3.47 QQNSDD17 pKa = 3.02 TDD19 pKa = 4.05 SYY21 pKa = 12.13 GDD23 pKa = 4.06 ACDD26 pKa = 3.63 NCPNVPNSSQKK37 pKa = 9.86 DD38 pKa = 3.41 TDD40 pKa = 3.83 GNGAGDD46 pKa = 3.66 TCDD49 pKa = 3.98 NDD51 pKa = 3.16 IDD53 pKa = 4.83 GDD55 pKa = 4.36 GIPNVLDD62 pKa = 3.65 NCPKK66 pKa = 10.25 VPNPMQTDD74 pKa = 3.36 RR75 pKa = 11.84 DD76 pKa = 3.6 GDD78 pKa = 4.04 RR79 pKa = 11.84 VGDD82 pKa = 4.0 ACDD85 pKa = 3.35 SCPEE89 pKa = 4.23 VNDD92 pKa = 4.05 PMQSDD97 pKa = 3.79 MDD99 pKa = 4.0 NDD101 pKa = 4.09 LVGDD105 pKa = 3.95 VCDD108 pKa = 4.47 TNQDD112 pKa = 3.17 MDD114 pKa = 4.21 GDD116 pKa = 4.14 GHH118 pKa = 6.78 QDD120 pKa = 3.62 SRR122 pKa = 11.84 DD123 pKa = 3.61 NCPDD127 pKa = 3.27 VPNSSQLDD135 pKa = 3.58 SDD137 pKa = 3.72 NDD139 pKa = 4.86 GIGDD143 pKa = 3.95 EE144 pKa = 5.45 CDD146 pKa = 5.19 DD147 pKa = 5.83 DD148 pKa = 6.4 DD149 pKa = 7.03 DD150 pKa = 6.24 NDD152 pKa = 5.35 GIPDD156 pKa = 3.89 TRR158 pKa = 11.84 PPGPDD163 pKa = 2.91 NCRR166 pKa = 11.84 LIPNPSQTDD175 pKa = 3.2 TDD177 pKa = 4.28 GNGVGDD183 pKa = 3.63 MCEE186 pKa = 3.88 NDD188 pKa = 3.53 FDD190 pKa = 4.34 NDD192 pKa = 4.02 SVIDD196 pKa = 4.89 LIDD199 pKa = 3.48 VCPEE203 pKa = 3.75 SAEE206 pKa = 3.87 VTMTDD211 pKa = 3.44 FRR213 pKa = 11.84 AYY215 pKa = 8.36 QTVILDD221 pKa = 3.91 PEE223 pKa = 5.2 GDD225 pKa = 3.69 AQIDD229 pKa = 4.02 PNWIVLNRR237 pKa = 11.84 GMEE240 pKa = 4.2 IVQTMNSDD248 pKa = 3.24 PGLAIGYY255 pKa = 6.53 TAFNGVDD262 pKa = 3.99 FEE264 pKa = 4.94 GTFHH268 pKa = 7.41 INTATDD274 pKa = 3.36 DD275 pKa = 4.34 DD276 pKa = 4.73 YY277 pKa = 12.04 VGFIFGYY284 pKa = 9.23 QDD286 pKa = 2.88 SSSFYY291 pKa = 10.16 VYY293 pKa = 10.6 LRR295 pKa = 11.84 NALWHH300 pKa = 6.31 TGDD303 pKa = 3.47 TPGEE307 pKa = 4.12 VTLLWKK313 pKa = 10.49 DD314 pKa = 3.4 PRR316 pKa = 11.84 NVGWKK321 pKa = 10.34 DD322 pKa = 2.99 KK323 pKa = 10.84 TSYY326 pKa = 10.02 RR327 pKa = 11.84 WHH329 pKa = 6.91 LSHH332 pKa = 7.13 RR333 pKa = 11.84 PQVGYY338 pKa = 10.62 INVRR342 pKa = 11.84 LYY344 pKa = 11.3 EE345 pKa = 4.1 GMTLVADD352 pKa = 3.69 SGMVVDD358 pKa = 3.88 TSMRR362 pKa = 11.84 GGRR365 pKa = 11.84 LGVFCFSQEE374 pKa = 3.83 QIIWSNLGYY383 pKa = 10.17 RR384 pKa = 11.84 CNDD387 pKa = 3.6 TVPEE391 pKa = 4.4 DD392 pKa = 3.44 YY393 pKa = 10.91 EE394 pKa = 4.55 FYY396 pKa = 10.6 RR397 pKa = 11.84 KK398 pKa = 9.56 QIHH401 pKa = 5.42 VRR403 pKa = 11.84 VV404 pKa = 3.77
Molecular weight: 44.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.664
IPC2_protein 3.668
IPC_protein 3.732
Toseland 3.478
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.389
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.859
Patrickios 1.189
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A6F8ZXA9|A0A6F8ZXA9_9TELE Uncharacterized protein (Fragment) OS=Coregonus sp. 'balchen' OX=861768 GN=CSTEINMANNI_LOCUS508758 PE=4 SV=1
TT1 pKa = 7.13 LRR3 pKa = 11.84 NPGLIINSPVTLRR16 pKa = 11.84 NPGLIINSPVTLRR29 pKa = 11.84 NPGLIINSPVTLRR42 pKa = 11.84 NPGLIINSPVTLRR55 pKa = 11.84 NPGLIINPPVTLRR68 pKa = 11.84 NPGLIINSPVTLRR81 pKa = 11.84 NPGLITNSPVTLRR94 pKa = 11.84 NPGLIINPPVTIRR107 pKa = 11.84 NPGLITNPPKK117 pKa = 10.27 PWTNHH122 pKa = 4.38 QPSITLRR129 pKa = 11.84 NPGLITNPPVTLRR142 pKa = 11.84 NPGLIINPPVTLRR155 pKa = 11.84 NPGLIINSPVTLRR168 pKa = 11.84 NPGLIINPPVTLRR181 pKa = 11.84 NPGLIINPPLPSEE194 pKa = 4.15 NPGLIINPPVTLRR207 pKa = 11.84 NPGLIINPPVTLRR220 pKa = 11.84 NPGLIINSPVTLRR233 pKa = 11.84 NPGLIINPPVTLRR246 pKa = 11.84 NPGLIINPPVTLRR259 pKa = 11.84 NPGLITNPPVTLRR272 pKa = 11.84 NPGLIINPPVTLRR285 pKa = 11.84 NPGLIINPPVTLRR298 pKa = 11.84 NPGLITNPPVTLRR311 pKa = 11.84 NPGLIINPPVTLRR324 pKa = 11.84 NPGLIINPPVTLRR337 pKa = 11.84 NPGLIINPPVTLRR350 pKa = 11.84 NPGLIINPPVTLRR363 pKa = 11.84 NPGLIINPP371 pKa = 3.92
Molecular weight: 39.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.618
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.325
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.267
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
41641
0
41641
18652202
9
12605
447.9
49.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.329 ± 0.013
2.203 ± 0.011
5.084 ± 0.01
6.927 ± 0.019
3.293 ± 0.008
6.763 ± 0.016
2.743 ± 0.009
4.111 ± 0.01
5.275 ± 0.016
9.404 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.005
3.705 ± 0.008
6.234 ± 0.02
4.78 ± 0.013
5.797 ± 0.012
8.705 ± 0.017
5.967 ± 0.016
6.352 ± 0.013
1.165 ± 0.005
2.703 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here