Vibrio virus VP882
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A2I2Z6|A2I2Z6_9CAUD Putative exonuclease OS=Vibrio virus VP882 OX=261726 PE=4 SV=1
MM1 pKa = 8.36 DD2 pKa = 4.85 YY3 pKa = 11.23 FDD5 pKa = 4.68 LQQLAGAALGLTEE18 pKa = 4.57 EE19 pKa = 4.3 QTDD22 pKa = 4.61 AIIDD26 pKa = 3.95 DD27 pKa = 5.4 DD28 pKa = 4.86 EE29 pKa = 7.02 DD30 pKa = 5.12 FDD32 pKa = 4.55 TPLMDD37 pKa = 4.23 KK38 pKa = 10.84 FGVDD42 pKa = 3.92 FDD44 pKa = 4.1 QFSEE48 pKa = 4.63 VAQALLPLTPTVQSPLTGVVSHH70 pKa = 6.73 AFVRR74 pKa = 11.84 QLGGGNCLAIAKK86 pKa = 8.57 MNAKK90 pKa = 8.38 EE91 pKa = 4.0 QPKK94 pKa = 9.98 KK95 pKa = 10.77 EE96 pKa = 3.95 EE97 pKa = 4.28 SACC100 pKa = 3.86
Molecular weight: 10.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.236
IPC2_protein 3.923
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.821
Sillero 3.999
Patrickios 3.63
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|A2I313|A2I313_9CAUD Uncharacterized protein OS=Vibrio virus VP882 OX=261726 PE=4 SV=1
MM1 pKa = 7.47 EE2 pKa = 6.18 LDD4 pKa = 3.66 SVKK7 pKa = 10.59 QGSAWNLHH15 pKa = 5.84 WGHH18 pKa = 7.47 AGMKK22 pKa = 9.68 RR23 pKa = 11.84 PRR25 pKa = 11.84 LSSRR29 pKa = 11.84 RR30 pKa = 11.84 ISGAVPQCVQFIALGNVSVTNHH52 pKa = 5.58 LHH54 pKa = 6.49 HH55 pKa = 7.22 LGQGRR60 pKa = 11.84 TVEE63 pKa = 4.33 LSQDD67 pKa = 3.89 LLHH70 pKa = 6.83 SSPARR75 pKa = 11.84 QPVTPPVSLWHH86 pKa = 6.73 ASQLANAA93 pKa = 4.96
Molecular weight: 10.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.428
IPC_protein 10.306
Toseland 10.95
ProMoST 10.906
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.023
Grimsley 10.994
Solomon 11.257
Lehninger 11.199
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.935
Patrickios 10.906
IPC_peptide 11.257
IPC2_peptide 10.087
IPC2.peptide.svr19 8.975
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
13769
38
1239
193.9
21.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.371 ± 0.469
1.322 ± 0.199
5.883 ± 0.273
6.602 ± 0.315
3.137 ± 0.184
7.176 ± 0.338
2.397 ± 0.214
4.735 ± 0.24
5.171 ± 0.295
8.73 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.702 ± 0.166
3.486 ± 0.182
4.481 ± 0.271
4.612 ± 0.165
6.769 ± 0.302
5.752 ± 0.271
5.556 ± 0.257
7.074 ± 0.307
1.482 ± 0.17
2.564 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here