Pseudomonas phage Pf3 (Bacteriophage Pf3)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Tertilicivirus; Pseudomonas virus Pf3

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03625|G6P_BPPF3 Head virion protein G6P OS=Pseudomonas phage Pf3 OX=10872 GN=VI PE=3 SV=1
MM1 pKa = 7.41GLHH4 pKa = 6.01YY5 pKa = 11.01LFGFCLALFSFSAIAAGPVSTEE27 pKa = 3.79VAAGTTTYY35 pKa = 10.75RR36 pKa = 11.84VTNTTVRR43 pKa = 11.84TPPNVTLSPVRR54 pKa = 11.84DD55 pKa = 3.3ITPYY59 pKa = 10.38VEE61 pKa = 4.99KK62 pKa = 10.58IPNKK66 pKa = 10.25GLAQAAQGRR75 pKa = 11.84LIVAQRR81 pKa = 11.84AASVPVTGFFNVSGAVVKK99 pKa = 10.88SGAKK103 pKa = 9.83SFLRR107 pKa = 11.84SAGRR111 pKa = 11.84ASGIGLGLAALLEE124 pKa = 4.38AADD127 pKa = 3.63WVFDD131 pKa = 3.88EE132 pKa = 5.02EE133 pKa = 4.9GEE135 pKa = 4.35IVKK138 pKa = 9.23PLPGGGSPVLMPRR151 pKa = 11.84PVILNEE157 pKa = 3.81YY158 pKa = 8.63TVTGSAGQWSISKK171 pKa = 10.01EE172 pKa = 4.01YY173 pKa = 10.64EE174 pKa = 3.66PDD176 pKa = 3.49PRR178 pKa = 11.84SVPGWYY184 pKa = 9.59SYY186 pKa = 11.22NGNPVWVSAVEE197 pKa = 4.26DD198 pKa = 3.56VGFTWRR204 pKa = 11.84YY205 pKa = 7.74WYY207 pKa = 10.32FADD210 pKa = 5.03VLMDD214 pKa = 3.55GQGRR218 pKa = 11.84PNYY221 pKa = 9.67LVAYY225 pKa = 9.32SDD227 pKa = 3.91SGPNEE232 pKa = 3.66YY233 pKa = 9.25WQDD236 pKa = 3.27VGGYY240 pKa = 9.57SLDD243 pKa = 3.76SLPTEE248 pKa = 4.31PEE250 pKa = 4.11FVPLTDD256 pKa = 4.23AEE258 pKa = 4.42LEE260 pKa = 4.15AGIDD264 pKa = 3.39QYY266 pKa = 11.69YY267 pKa = 10.45EE268 pKa = 4.38PDD270 pKa = 3.97PDD272 pKa = 3.15DD273 pKa = 3.43WRR275 pKa = 11.84NLFPYY280 pKa = 10.0IEE282 pKa = 5.08PDD284 pKa = 2.96SFTIEE289 pKa = 4.13TPIPSLDD296 pKa = 3.53LSPVVSSSTNNQTGKK311 pKa = 8.66VTVTEE316 pKa = 4.43TTTSVDD322 pKa = 4.82FEE324 pKa = 4.64VSDD327 pKa = 4.29NNSSQPSISVNEE339 pKa = 4.15TTTEE343 pKa = 3.75NVYY346 pKa = 10.89VDD348 pKa = 3.76GDD350 pKa = 4.16LVSSEE355 pKa = 4.39TNTTVTNPPSSGTSTPPSSGSGSDD379 pKa = 3.46FQLPSFCSWATAVCDD394 pKa = 3.54WFDD397 pKa = 3.41WTQEE401 pKa = 4.48PIDD404 pKa = 4.64EE405 pKa = 4.66EE406 pKa = 5.02PDD408 pKa = 3.17LSGIISDD415 pKa = 3.69IDD417 pKa = 3.41DD418 pKa = 4.04LEE420 pKa = 4.23RR421 pKa = 11.84TKK423 pKa = 10.89DD424 pKa = 3.19ISFGSKK430 pKa = 9.61SCPAPIALDD439 pKa = 3.43IEE441 pKa = 4.39FLDD444 pKa = 3.73MSVDD448 pKa = 4.61LSFEE452 pKa = 4.31WFCEE456 pKa = 3.79LAGIIYY462 pKa = 9.03FMVMASAYY470 pKa = 10.25VLAAYY475 pKa = 7.31ITLGVVRR482 pKa = 11.84GG483 pKa = 3.72

Molecular weight: 52.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03627-2|REP-2_BPPF3 Isoform of P03627 Isoform G10P of Replication-associated protein G2P OS=Pseudomonas phage Pf3 OX=10872 GN=II PE=4 SV=1
MM1 pKa = 7.75GDD3 pKa = 2.89ATFYY7 pKa = 9.38QLRR10 pKa = 11.84RR11 pKa = 11.84IVKK14 pKa = 8.07RR15 pKa = 11.84WRR17 pKa = 11.84FADD20 pKa = 3.31ARR22 pKa = 11.84ASGSIHH28 pKa = 7.69RR29 pKa = 11.84IRR31 pKa = 11.84SAKK34 pKa = 10.11KK35 pKa = 7.82YY36 pKa = 9.23LQVPEE41 pKa = 4.14TLSRR45 pKa = 11.84VLGASEE51 pKa = 3.71WMPEE55 pKa = 3.79NEE57 pKa = 4.81LLSILYY63 pKa = 8.99FLKK66 pKa = 10.18SQGYY70 pKa = 8.89EE71 pKa = 3.97FLL73 pKa = 5.21

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

1909

44

483

190.9

20.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.539 ± 0.695

1.257 ± 0.295

5.186 ± 0.658

4.872 ± 0.525

4.819 ± 0.422

6.967 ± 0.377

2.095 ± 0.692

5.395 ± 0.539

3.248 ± 0.572

9.953 ± 1.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.938 ± 0.231

3.248 ± 0.332

5.605 ± 0.766

3.353 ± 0.417

5.186 ± 1.058

9.115 ± 0.765

5.605 ± 0.695

8.853 ± 0.779

1.886 ± 0.477

2.881 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski