Lachnospiraceae bacterium M18-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9JRF0|R9JRF0_9FIRM ABC transporter domain-containing protein OS=Lachnospiraceae bacterium M18-1 OX=1235792 GN=C808_03980 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.43KK3 pKa = 10.23KK4 pKa = 10.47LLSVLLAGVMVISLAACGGGSGDD27 pKa = 4.19GGSGDD32 pKa = 3.79SDD34 pKa = 3.44GGSGTTAEE42 pKa = 4.58SDD44 pKa = 4.16DD45 pKa = 4.03GGADD49 pKa = 3.27SGSEE53 pKa = 4.1GGHH56 pKa = 6.77KK57 pKa = 9.42LTVWTWDD64 pKa = 3.35PAFNIPAIKK73 pKa = 9.92EE74 pKa = 3.63AGEE77 pKa = 4.0IYY79 pKa = 10.15KK80 pKa = 10.72AEE82 pKa = 3.95VDD84 pKa = 3.93SEE86 pKa = 4.59FEE88 pKa = 4.26LEE90 pKa = 4.38VIEE93 pKa = 4.72TLSDD97 pKa = 3.54DD98 pKa = 5.17CEE100 pKa = 4.58TKK102 pKa = 10.79LQTCAEE108 pKa = 4.23SGDD111 pKa = 4.07FGTMADD117 pKa = 4.39IILMQDD123 pKa = 2.56NSFQKK128 pKa = 10.27FVSFYY133 pKa = 10.6PEE135 pKa = 4.17AFTDD139 pKa = 3.89LTDD142 pKa = 3.44SGIEE146 pKa = 3.72FSEE149 pKa = 4.36FAEE152 pKa = 4.86GKK154 pKa = 9.59LAYY157 pKa = 9.46STVDD161 pKa = 3.04GKK163 pKa = 10.87NYY165 pKa = 10.06GVPFDD170 pKa = 3.85NGAVISCYY178 pKa = 9.61RR179 pKa = 11.84TDD181 pKa = 3.79ILDD184 pKa = 3.5QAGYY188 pKa = 8.83TIDD191 pKa = 5.13DD192 pKa = 4.21LTDD195 pKa = 3.61IDD197 pKa = 3.54WDD199 pKa = 3.72KK200 pKa = 11.18FIEE203 pKa = 4.07IGKK206 pKa = 6.48TVKK209 pKa = 10.41EE210 pKa = 4.0KK211 pKa = 9.64TGKK214 pKa = 10.83YY215 pKa = 8.69MLSGQANSQDD225 pKa = 3.93IIMMMLQSAGASLFNEE241 pKa = 4.74DD242 pKa = 3.54GTPNLVGNDD251 pKa = 3.88ALNEE255 pKa = 4.55CIDD258 pKa = 4.09IYY260 pKa = 11.35QKK262 pKa = 9.38MVKK265 pKa = 9.96EE266 pKa = 4.57GIFYY270 pKa = 10.12EE271 pKa = 4.28VNEE274 pKa = 3.76WDD276 pKa = 4.43EE277 pKa = 4.41YY278 pKa = 11.13VSSITNEE285 pKa = 3.71QCAGTINGNWIMATIMGMEE304 pKa = 4.25DD305 pKa = 3.2TSGNWAITNMPKK317 pKa = 10.66LMNASGATNYY327 pKa = 10.59SNNGGSSWYY336 pKa = 9.79ISSNCQNTDD345 pKa = 3.18LAFDD349 pKa = 4.06FMAKK353 pKa = 8.94TFAGSVTLYY362 pKa = 11.03EE363 pKa = 4.79NILPTTGAISTWGPAGDD380 pKa = 3.95SEE382 pKa = 5.92AYY384 pKa = 9.2QAPQEE389 pKa = 4.24FWSDD393 pKa = 3.39QPIFATVVEE402 pKa = 4.99FAEE405 pKa = 4.47KK406 pKa = 9.85TPSNNTSAYY415 pKa = 8.36YY416 pKa = 9.76YY417 pKa = 10.16DD418 pKa = 3.54VRR420 pKa = 11.84EE421 pKa = 4.41VISTAIQDD429 pKa = 3.43IMSGGGEE436 pKa = 3.67KK437 pKa = 10.53DD438 pKa = 3.8AILEE442 pKa = 3.99QAQADD447 pKa = 3.83AEE449 pKa = 4.47FYY451 pKa = 10.13YY452 pKa = 10.68QQ453 pKa = 4.31

Molecular weight: 48.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9K6U7|R9K6U7_9FIRM Hydroxyethylthiazole kinase OS=Lachnospiraceae bacterium M18-1 OX=1235792 GN=thiM PE=3 SV=1
MM1 pKa = 7.19NEE3 pKa = 3.78VMKK6 pKa = 10.88SLLSHH11 pKa = 5.57QSIRR15 pKa = 11.84AYY17 pKa = 8.76TEE19 pKa = 3.65QMVEE23 pKa = 4.01EE24 pKa = 4.26EE25 pKa = 5.18KK26 pKa = 10.76INQIIQAVQCRR37 pKa = 11.84QRR39 pKa = 11.84RR40 pKa = 11.84TGTTVQEE47 pKa = 4.05NGRR50 pKa = 11.84TLRR53 pKa = 11.84RR54 pKa = 11.84IFISIHH60 pKa = 3.69MTITRR65 pKa = 11.84KK66 pKa = 8.98FRR68 pKa = 11.84KK69 pKa = 9.5CC70 pKa = 3.14

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5263

0

5263

1644781

23

2742

312.5

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.463 ± 0.034

1.562 ± 0.015

5.527 ± 0.023

8.089 ± 0.038

4.124 ± 0.025

7.144 ± 0.031

1.81 ± 0.013

7.014 ± 0.034

6.527 ± 0.032

9.008 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.152 ± 0.017

4.036 ± 0.021

3.387 ± 0.021

3.51 ± 0.019

5.075 ± 0.028

5.766 ± 0.028

5.089 ± 0.027

6.461 ± 0.031

1.017 ± 0.011

4.241 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski