Megasphaera vaginalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U7UG60|U7UG60_9FIRM Glycosyltransferase group 2 family protein OS=Megasphaera vaginalis OX=1111454 GN=HMPREF1250_1135 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.14KK3 pKa = 11.08YY4 pKa = 10.24EE5 pKa = 4.77CSVCGYY11 pKa = 10.61VYY13 pKa = 10.94DD14 pKa = 4.3EE15 pKa = 5.45AEE17 pKa = 4.06GDD19 pKa = 3.6ADD21 pKa = 3.73NGVAAGTKK29 pKa = 9.76FADD32 pKa = 4.29LPEE35 pKa = 5.13DD36 pKa = 3.87WVCPICGADD45 pKa = 3.17KK46 pKa = 11.24DD47 pKa = 4.46SFNKK51 pKa = 10.29LL52 pKa = 3.11

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U7UCB8|U7UCB8_9FIRM PF04536 family protein OS=Megasphaera vaginalis OX=1111454 GN=HMPREF1250_1567 PE=4 SV=1
MM1 pKa = 7.1NQPKK5 pKa = 10.16KK6 pKa = 10.49IKK8 pKa = 9.13LQKK11 pKa = 8.59RR12 pKa = 11.84TRR14 pKa = 11.84SAARR18 pKa = 11.84IHH20 pKa = 6.68KK21 pKa = 8.72GLSMPVGSADD31 pKa = 3.56EE32 pKa = 4.26DD33 pKa = 4.5SIISFRR39 pKa = 11.84DD40 pKa = 3.56FGEE43 pKa = 4.04LKK45 pKa = 10.08EE46 pKa = 4.22KK47 pKa = 10.75YY48 pKa = 9.85FRR50 pKa = 11.84FSGTLQRR57 pKa = 11.84QPFVLRR63 pKa = 11.84TLVLMFGQAFFSLILYY79 pKa = 9.64SRR81 pKa = 11.84LVEE84 pKa = 3.94ALLIGRR90 pKa = 11.84IDD92 pKa = 3.73LALLFAVIFLALTVPVVWAQISVGSRR118 pKa = 11.84RR119 pKa = 11.84LRR121 pKa = 11.84DD122 pKa = 3.32MGRR125 pKa = 11.84SGWLFAVPFSSYY137 pKa = 9.41SCCYY141 pKa = 9.37ILPVLGAAAYY151 pKa = 7.41WQFAAAATVVLYY163 pKa = 9.71FLLALVKK170 pKa = 9.45GTAAAEE176 pKa = 3.85PRR178 pKa = 11.84RR179 pKa = 11.84RR180 pKa = 3.58

Molecular weight: 20.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2196

0

2196

657555

35

2954

299.4

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.818 ± 0.07

1.351 ± 0.024

5.505 ± 0.038

6.183 ± 0.061

3.948 ± 0.04

7.746 ± 0.057

2.048 ± 0.027

6.644 ± 0.048

5.517 ± 0.051

9.52 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.027

3.576 ± 0.045

3.719 ± 0.034

3.358 ± 0.031

5.364 ± 0.052

5.39 ± 0.044

5.687 ± 0.046

7.321 ± 0.048

0.952 ± 0.02

3.565 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski