Amino acid dipepetide frequency for Firmicutes bacterium CAG:114

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.119AlaAla: 11.119 ± 0.173	1.585AlaCys: 1.585 ± 0.052	4.843AlaAsp: 4.843 ± 0.096	5.751AlaGlu: 5.751 ± 0.104	3.525AlaPhe: 3.525 ± 0.082	8.284AlaGly: 8.284 ± 0.126	1.608AlaHis: 1.608 ± 0.055	4.923AlaIle: 4.923 ± 0.111	4.028AlaLys: 4.028 ± 0.091	10.832AlaLeu: 10.832 ± 0.164	2.795AlaMet: 2.795 ± 0.068	2.349AlaAsn: 2.349 ± 0.071	3.525AlaPro: 3.525 ± 0.077	4.814AlaGln: 4.814 ± 0.104	5.404AlaArg: 5.404 ± 0.1	4.353AlaSer: 4.353 ± 0.085	3.895AlaThr: 3.895 ± 0.1	7.851AlaVal: 7.851 ± 0.129	1.07AlaTrp: 1.07 ± 0.042	2.866AlaTyr: 2.866 ± 0.063	0.002AlaXaa: 0.002 ± 0.001
Cys	1.476CysAla: 1.476 ± 0.054	0.325CysCys: 0.325 ± 0.028	0.8CysAsp: 0.8 ± 0.04	0.659CysGlu: 0.659 ± 0.034	0.576CysPhe: 0.576 ± 0.029	1.58CysGly: 1.58 ± 0.054	0.358CysHis: 0.358 ± 0.024	0.732CysIle: 0.732 ± 0.032	0.52CysLys: 0.52 ± 0.029	1.72CysLeu: 1.72 ± 0.056	0.347CysMet: 0.347 ± 0.026	0.448CysAsn: 0.448 ± 0.028	0.834CysPro: 0.834 ± 0.042	0.652CysGln: 0.652 ± 0.028	1.015CysArg: 1.015 ± 0.043	0.814CysSer: 0.814 ± 0.038	0.906CysThr: 0.906 ± 0.037	1.154CysVal: 1.154 ± 0.043	0.167CysTrp: 0.167 ± 0.017	0.532CysTyr: 0.532 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.37AspAla: 4.37 ± 0.093	0.78AspCys: 0.78 ± 0.032	2.189AspAsp: 2.189 ± 0.07	2.941AspGlu: 2.941 ± 0.074	2.145AspPhe: 2.145 ± 0.063	4.49AspGly: 4.49 ± 0.108	1.208AspHis: 1.208 ± 0.049	2.846AspIle: 2.846 ± 0.072	2.19AspLys: 2.19 ± 0.065	5.841AspLeu: 5.841 ± 0.102	1.34AspMet: 1.34 ± 0.042	1.499AspAsn: 1.499 ± 0.05	2.696AspPro: 2.696 ± 0.062	2.357AspGln: 2.357 ± 0.062	3.23AspArg: 3.23 ± 0.076	2.41AspSer: 2.41 ± 0.074	3.026AspThr: 3.026 ± 0.082	3.47AspVal: 3.47 ± 0.078	0.637AspTrp: 0.637 ± 0.033	2.109AspTyr: 2.109 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	6.366GluAla: 6.366 ± 0.116	0.523GluCys: 0.523 ± 0.026	3.707GluAsp: 3.707 ± 0.084	6.319GluGlu: 6.319 ± 0.126	1.779GluPhe: 1.779 ± 0.056	5.175GluGly: 5.175 ± 0.09	1.224GluHis: 1.224 ± 0.048	3.967GluIle: 3.967 ± 0.088	4.191GluLys: 4.191 ± 0.109	5.7GluLeu: 5.7 ± 0.107	1.857GluMet: 1.857 ± 0.054	2.639GluAsn: 2.639 ± 0.074	2.015GluPro: 2.015 ± 0.061	2.728GluGln: 2.728 ± 0.068	3.36GluArg: 3.36 ± 0.082	2.729GluSer: 2.729 ± 0.067	4.252GluThr: 4.252 ± 0.095	4.059GluVal: 4.059 ± 0.093	0.448GluTrp: 0.448 ± 0.027	1.885GluTyr: 1.885 ± 0.062	0.002GluXaa: 0.002 ± 0.002
Phe	3.265PheAla: 3.265 ± 0.072	0.791PheCys: 0.791 ± 0.038	1.971PheAsp: 1.971 ± 0.056	1.605PheGlu: 1.605 ± 0.051	1.835PhePhe: 1.835 ± 0.061	2.974PheGly: 2.974 ± 0.069	0.939PheHis: 0.939 ± 0.04	1.555PheIle: 1.555 ± 0.057	0.97PheLys: 0.97 ± 0.042	4.614PheLeu: 4.614 ± 0.103	0.752PheMet: 0.752 ± 0.037	0.948PheAsn: 0.948 ± 0.04	1.804PhePro: 1.804 ± 0.06	1.795PheGln: 1.795 ± 0.052	2.097PheArg: 2.097 ± 0.061	2.967PheSer: 2.967 ± 0.075	2.215PheThr: 2.215 ± 0.066	2.471PheVal: 2.471 ± 0.061	0.473PheTrp: 0.473 ± 0.03	1.392PheTyr: 1.392 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	6.895GlyAla: 6.895 ± 0.124	1.337GlyCys: 1.337 ± 0.051	4.113GlyAsp: 4.113 ± 0.094	5.69GlyGlu: 5.69 ± 0.122	3.072GlyPhe: 3.072 ± 0.081	6.995GlyGly: 6.995 ± 0.159	1.59GlyHis: 1.59 ± 0.057	5.226GlyIle: 5.226 ± 0.108	4.521GlyLys: 4.521 ± 0.084	8.198GlyLeu: 8.198 ± 0.135	2.478GlyMet: 2.478 ± 0.059	2.432GlyAsn: 2.432 ± 0.066	2.542GlyPro: 2.542 ± 0.105	3.402GlyGln: 3.402 ± 0.078	4.518GlyArg: 4.518 ± 0.087	4.224GlySer: 4.224 ± 0.094	5.334GlyThr: 5.334 ± 0.116	6.296GlyVal: 6.296 ± 0.099	0.948GlyTrp: 0.948 ± 0.046	3.011GlyTyr: 3.011 ± 0.078	0.011GlyXaa: 0.011 ± 0.004
His	1.501HisAla: 1.501 ± 0.047	0.406HisCys: 0.406 ± 0.027	0.856HisAsp: 0.856 ± 0.039	0.864HisGlu: 0.864 ± 0.04	0.813HisPhe: 0.813 ± 0.041	1.809HisGly: 1.809 ± 0.056	0.606HisHis: 0.606 ± 0.043	1.161HisIle: 1.161 ± 0.051	0.668HisLys: 0.668 ± 0.031	2.131HisLeu: 2.131 ± 0.06	0.498HisMet: 0.498 ± 0.026	0.632HisAsn: 0.632 ± 0.031	1.454HisPro: 1.454 ± 0.057	0.864HisGln: 0.864 ± 0.04	1.278HisArg: 1.278 ± 0.05	0.948HisSer: 0.948 ± 0.04	1.113HisThr: 1.113 ± 0.047	1.214HisVal: 1.214 ± 0.048	0.336HisTrp: 0.336 ± 0.023	0.676HisTyr: 0.676 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.089IleAla: 5.089 ± 0.09	0.889IleCys: 0.889 ± 0.037	2.731IleAsp: 2.731 ± 0.07	2.542IleGlu: 2.542 ± 0.078	2.055IlePhe: 2.055 ± 0.062	3.956IleGly: 3.956 ± 0.08	1.121IleHis: 1.121 ± 0.039	2.536IleIle: 2.536 ± 0.08	1.843IleLys: 1.843 ± 0.064	6.403IleLeu: 6.403 ± 0.118	1.076IleMet: 1.076 ± 0.04	1.477IleAsn: 1.477 ± 0.047	2.871IlePro: 2.871 ± 0.068	2.387IleGln: 2.387 ± 0.054	3.237IleArg: 3.237 ± 0.075	3.269IleSer: 3.269 ± 0.072	3.383IleThr: 3.383 ± 0.078	3.694IleVal: 3.694 ± 0.09	0.504IleTrp: 0.504 ± 0.031	1.576IleTyr: 1.576 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.091LysAla: 4.091 ± 0.097	0.445LysCys: 0.445 ± 0.029	2.279LysAsp: 2.279 ± 0.065	3.439LysGlu: 3.439 ± 0.086	1.132LysPhe: 1.132 ± 0.047	3.539LysGly: 3.539 ± 0.074	0.794LysHis: 0.794 ± 0.035	2.469LysIle: 2.469 ± 0.078	3.064LysLys: 3.064 ± 0.083	4.052LysLeu: 4.052 ± 0.085	1.211LysMet: 1.211 ± 0.044	1.737LysAsn: 1.737 ± 0.058	1.714LysPro: 1.714 ± 0.061	1.689LysGln: 1.689 ± 0.055	2.458LysArg: 2.458 ± 0.059	2.049LysSer: 2.049 ± 0.062	2.86LysThr: 2.86 ± 0.081	3.097LysVal: 3.097 ± 0.084	0.339LysTrp: 0.339 ± 0.024	1.487LysTyr: 1.487 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	10.474LeuAla: 10.474 ± 0.153	2.187LeuCys: 2.187 ± 0.062	5.639LeuAsp: 5.639 ± 0.093	6.648LeuGlu: 6.648 ± 0.124	4.256LeuPhe: 4.256 ± 0.095	8.181LeuGly: 8.181 ± 0.136	2.066LeuHis: 2.066 ± 0.062	4.677LeuIle: 4.677 ± 0.088	3.847LeuLys: 3.847 ± 0.085	12.187LeuLeu: 12.187 ± 0.219	2.549LeuMet: 2.549 ± 0.071	2.724LeuAsn: 2.724 ± 0.075	5.572LeuPro: 5.572 ± 0.104	3.691LeuGln: 3.691 ± 0.083	6.347LeuArg: 6.347 ± 0.113	7.443LeuSer: 7.443 ± 0.131	6.467LeuThr: 6.467 ± 0.102	6.858LeuVal: 6.858 ± 0.12	1.238LeuTrp: 1.238 ± 0.05	3.148LeuTyr: 3.148 ± 0.082	0.005LeuXaa: 0.005 ± 0.003
Met	2.72MetAla: 2.72 ± 0.059	0.274MetCys: 0.274 ± 0.021	1.604MetAsp: 1.604 ± 0.053	2.181MetGlu: 2.181 ± 0.063	0.68MetPhe: 0.68 ± 0.033	2.248MetGly: 2.248 ± 0.058	0.353MetHis: 0.353 ± 0.022	1.325MetIle: 1.325 ± 0.049	1.613MetLys: 1.613 ± 0.055	2.524MetLeu: 2.524 ± 0.067	0.665MetMet: 0.665 ± 0.037	1.004MetAsn: 1.004 ± 0.044	1.066MetPro: 1.066 ± 0.044	0.769MetGln: 0.769 ± 0.033	1.292MetArg: 1.292 ± 0.044	1.323MetSer: 1.323 ± 0.04	1.737MetThr: 1.737 ± 0.05	2.044MetVal: 2.044 ± 0.053	0.148MetTrp: 0.148 ± 0.015	0.585MetTyr: 0.585 ± 0.031	0.002MetXaa: 0.002 ± 0.002
Asn	2.658AsnAla: 2.658 ± 0.068	0.458AsnCys: 0.458 ± 0.026	1.33AsnAsp: 1.33 ± 0.05	1.504AsnGlu: 1.504 ± 0.053	1.088AsnPhe: 1.088 ± 0.042	2.787AsnGly: 2.787 ± 0.088	0.637AsnHis: 0.637 ± 0.036	1.787AsnIle: 1.787 ± 0.057	1.23AsnLys: 1.23 ± 0.052	3.215AsnLeu: 3.215 ± 0.077	0.755AsnMet: 0.755 ± 0.035	1.062AsnAsn: 1.062 ± 0.054	1.96AsnPro: 1.96 ± 0.055	1.278AsnGln: 1.278 ± 0.046	1.842AsnArg: 1.842 ± 0.051	1.396AsnSer: 1.396 ± 0.053	1.835AsnThr: 1.835 ± 0.064	2.047AsnVal: 2.047 ± 0.063	0.361AsnTrp: 0.361 ± 0.024	1.136AsnTyr: 1.136 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	4.734ProAla: 4.734 ± 0.085	0.588ProCys: 0.588 ± 0.03	2.888ProAsp: 2.888 ± 0.071	3.652ProGlu: 3.652 ± 0.075	1.731ProPhe: 1.731 ± 0.054	3.774ProGly: 3.774 ± 0.094	0.917ProHis: 0.917 ± 0.034	1.991ProIle: 1.991 ± 0.051	1.698ProLys: 1.698 ± 0.056	4.188ProLeu: 4.188 ± 0.09	1.286ProMet: 1.286 ± 0.048	1.348ProAsn: 1.348 ± 0.051	1.927ProPro: 1.927 ± 0.071	2.035ProGln: 2.035 ± 0.07	2.044ProArg: 2.044 ± 0.071	2.2ProSer: 2.2 ± 0.063	2.13ProThr: 2.13 ± 0.062	3.942ProVal: 3.942 ± 0.085	0.504ProTrp: 0.504 ± 0.031	1.409ProTyr: 1.409 ± 0.053	0.005ProXaa: 0.005 ± 0.003
Gln	5.134GlnAla: 5.134 ± 0.102	0.472GlnCys: 0.472 ± 0.027	2.021GlnAsp: 2.021 ± 0.055	3.279GlnGlu: 3.279 ± 0.102	1.308GlnPhe: 1.308 ± 0.048	3.64GlnGly: 3.64 ± 0.093	0.607GlnHis: 0.607 ± 0.031	2.111GlnIle: 2.111 ± 0.056	2.021GlnLys: 2.021 ± 0.068	3.789GlnLeu: 3.789 ± 0.089	1.185GlnMet: 1.185 ± 0.04	1.277GlnAsn: 1.277 ± 0.042	1.586GlnPro: 1.586 ± 0.049	1.635GlnGln: 1.635 ± 0.047	2.558GlnArg: 2.558 ± 0.062	1.84GlnSer: 1.84 ± 0.059	2.338GlnThr: 2.338 ± 0.06	3.458GlnVal: 3.458 ± 0.085	0.564GlnTrp: 0.564 ± 0.031	1.267GlnTyr: 1.267 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	5.016ArgAla: 5.016 ± 0.106	0.844ArgCys: 0.844 ± 0.036	2.843ArgAsp: 2.843 ± 0.071	4.465ArgGlu: 4.465 ± 0.089	2.083ArgPhe: 2.083 ± 0.059	3.973ArgGly: 3.973 ± 0.091	1.157ArgHis: 1.157 ± 0.048	2.812ArgIle: 2.812 ± 0.068	2.88ArgLys: 2.88 ± 0.078	5.776ArgLeu: 5.776 ± 0.113	1.681ArgMet: 1.681 ± 0.047	1.82ArgAsn: 1.82 ± 0.065	2.549ArgPro: 2.549 ± 0.059	2.992ArgGln: 2.992 ± 0.078	4.407ArgArg: 4.407 ± 0.096	2.552ArgSer: 2.552 ± 0.07	3.011ArgThr: 3.011 ± 0.069	3.775ArgVal: 3.775 ± 0.067	0.682ArgTrp: 0.682 ± 0.037	1.941ArgTyr: 1.941 ± 0.057	0.005ArgXaa: 0.005 ± 0.003
Ser	4.789SerAla: 4.789 ± 0.093	0.811SerCys: 0.811 ± 0.037	2.545SerAsp: 2.545 ± 0.073	2.527SerGlu: 2.527 ± 0.067	2.398SerPhe: 2.398 ± 0.064	4.851SerGly: 4.851 ± 0.101	1.199SerHis: 1.199 ± 0.045	2.858SerIle: 2.858 ± 0.075	2.016SerLys: 2.016 ± 0.064	5.95SerLeu: 5.95 ± 0.111	1.471SerMet: 1.471 ± 0.048	1.596SerAsn: 1.596 ± 0.049	2.668SerPro: 2.668 ± 0.064	2.365SerGln: 2.365 ± 0.061	2.919SerArg: 2.919 ± 0.079	3.132SerSer: 3.132 ± 0.096	2.777SerThr: 2.777 ± 0.085	3.968SerVal: 3.968 ± 0.084	0.609SerTrp: 0.609 ± 0.033	1.876SerTyr: 1.876 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	5.7ThrAla: 5.7 ± 0.106	0.78ThrCys: 0.78 ± 0.035	2.813ThrAsp: 2.813 ± 0.069	3.277ThrGlu: 3.277 ± 0.084	2.318ThrPhe: 2.318 ± 0.057	5.452ThrGly: 5.452 ± 0.116	1.042ThrHis: 1.042 ± 0.043	3.395ThrIle: 3.395 ± 0.087	2.008ThrLys: 2.008 ± 0.056	6.455ThrLeu: 6.455 ± 0.101	1.496ThrMet: 1.496 ± 0.045	1.572ThrAsn: 1.572 ± 0.059	3.128ThrPro: 3.128 ± 0.085	2.156ThrGln: 2.156 ± 0.058	2.714ThrArg: 2.714 ± 0.072	2.706ThrSer: 2.706 ± 0.063	3.137ThrThr: 3.137 ± 0.086	5.222ThrVal: 5.222 ± 0.122	0.713ThrTrp: 0.713 ± 0.031	1.962ThrTyr: 1.962 ± 0.06	0.003ThrXaa: 0.003 ± 0.002
Val	6.283ValAla: 6.283 ± 0.113	1.386ValCys: 1.386 ± 0.053	3.895ValAsp: 3.895 ± 0.095	4.713ValGlu: 4.713 ± 0.098	2.832ValPhe: 2.832 ± 0.067	5.48ValGly: 5.48 ± 0.106	1.302ValHis: 1.302 ± 0.049	4.163ValIle: 4.163 ± 0.078	2.983ValLys: 2.983 ± 0.064	7.968ValLeu: 7.968 ± 0.119	1.759ValMet: 1.759 ± 0.051	2.324ValAsn: 2.324 ± 0.071	3.274ValPro: 3.274 ± 0.074	2.45ValGln: 2.45 ± 0.073	3.911ValArg: 3.911 ± 0.086	4.778ValSer: 4.778 ± 0.093	4.86ValThr: 4.86 ± 0.114	5.698ValVal: 5.698 ± 0.116	0.803ValTrp: 0.803 ± 0.039	2.332ValTyr: 2.332 ± 0.063	0.002ValXaa: 0.002 ± 0.002
Trp	0.953TrpAla: 0.953 ± 0.043	0.165TrpCys: 0.165 ± 0.015	0.56TrpAsp: 0.56 ± 0.027	0.791TrpGlu: 0.791 ± 0.037	0.445TrpPhe: 0.445 ± 0.029	0.892TrpGly: 0.892 ± 0.05	0.244TrpHis: 0.244 ± 0.018	0.551TrpIle: 0.551 ± 0.031	0.612TrpLys: 0.612 ± 0.03	1.099TrpLeu: 1.099 ± 0.047	0.355TrpMet: 0.355 ± 0.025	0.411TrpAsn: 0.411 ± 0.029	0.371TrpPro: 0.371 ± 0.02	0.484TrpGln: 0.484 ± 0.029	0.617TrpArg: 0.617 ± 0.036	0.601TrpSer: 0.601 ± 0.031	0.606TrpThr: 0.606 ± 0.032	0.729TrpVal: 0.729 ± 0.035	0.118TrpTrp: 0.118 ± 0.016	0.417TrpTyr: 0.417 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.777TyrAla: 2.777 ± 0.066	0.568TyrCys: 0.568 ± 0.027	2.147TyrAsp: 2.147 ± 0.067	2.072TyrGlu: 2.072 ± 0.058	1.364TyrPhe: 1.364 ± 0.044	2.752TyrGly: 2.752 ± 0.073	0.859TyrHis: 0.859 ± 0.037	1.554TyrIle: 1.554 ± 0.055	1.043TyrLys: 1.043 ± 0.043	3.559TyrLeu: 3.559 ± 0.086	0.599TyrMet: 0.599 ± 0.032	1.063TyrAsn: 1.063 ± 0.043	1.432TyrPro: 1.432 ± 0.049	1.546TyrGln: 1.546 ± 0.054	2.011TyrArg: 2.011 ± 0.058	1.59TyrSer: 1.59 ± 0.05	2.162TyrThr: 2.162 ± 0.062	2.15TyrVal: 2.15 ± 0.063	0.358TyrTrp: 0.358 ± 0.025	1.247TyrTyr: 1.247 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.009XaaAla: 0.009 ± 0.004	0.002XaaCys: 0.002 ± 0.001	0.003XaaAsp: 0.003 ± 0.002	0.005XaaGlu: 0.005 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.002	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.005XaaArg: 0.005 ± 0.003	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.033XaaXaa: 0.033 ± 0.009

Statistics based on 2222 proteins (642334 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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