Littorina sp. associated circular virus
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLM4|A0A0K1RLM4_9CIRC Putative replication initiation protein OS=Littorina sp. associated circular virus OX=1692253 PE=4 SV=1
MM1 pKa = 7.06 PAKK4 pKa = 9.85 RR5 pKa = 11.84 VYY7 pKa = 9.5 QGKK10 pKa = 9.9 RR11 pKa = 11.84 GKK13 pKa = 9.67 RR14 pKa = 11.84 WCFTLNNYY22 pKa = 8.26 TEE24 pKa = 4.25 AEE26 pKa = 4.07 KK27 pKa = 11.12 DD28 pKa = 3.76 VVVAGLIGKK37 pKa = 8.81 EE38 pKa = 3.74 PVFAKK43 pKa = 10.65 VGAEE47 pKa = 4.08 VGDD50 pKa = 3.62 SGTPHH55 pKa = 6.38 LQGFVHH61 pKa = 6.42 LKK63 pKa = 9.81 EE64 pKa = 4.29 RR65 pKa = 11.84 EE66 pKa = 4.08 SLGGVKK72 pKa = 10.12 KK73 pKa = 10.72 ILGNRR78 pKa = 11.84 CHH80 pKa = 8.77 LEE82 pKa = 3.82 MARR85 pKa = 11.84 GTDD88 pKa = 3.33 IEE90 pKa = 4.06 NDD92 pKa = 3.47 EE93 pKa = 4.38 YY94 pKa = 11.52 VSKK97 pKa = 11.12 GSIVVEE103 pKa = 4.2 LGAPVEE109 pKa = 4.38 GATEE113 pKa = 4.05 KK114 pKa = 11.18 GGGDD118 pKa = 3.05 TGYY121 pKa = 10.9 IMARR125 pKa = 11.84 AAAQQLADD133 pKa = 4.58 GGDD136 pKa = 3.39 LCAIAEE142 pKa = 4.56 DD143 pKa = 4.03 PEE145 pKa = 4.45 LWKK148 pKa = 10.68 SYY150 pKa = 8.55 CQHH153 pKa = 5.14 GRR155 pKa = 11.84 AIEE158 pKa = 4.07 KK159 pKa = 10.13 LAEE162 pKa = 4.03 ATRR165 pKa = 11.84 KK166 pKa = 9.72 SKK168 pKa = 9.55 QHH170 pKa = 5.36 QLAADD175 pKa = 3.78 SLGDD179 pKa = 4.55 PILQAWQRR187 pKa = 11.84 TLLRR191 pKa = 11.84 HH192 pKa = 5.45 VLGEE196 pKa = 3.6 PDD198 pKa = 3.02 MRR200 pKa = 11.84 KK201 pKa = 8.68 IHH203 pKa = 5.78 WMYY206 pKa = 11.42 DD207 pKa = 3.17 LVGNMGKK214 pKa = 7.63 TWMSKK219 pKa = 8.47 YY220 pKa = 10.63 LCAKK224 pKa = 8.94 HH225 pKa = 5.69 GAVRR229 pKa = 11.84 FEE231 pKa = 4.25 NGKK234 pKa = 9.75 SADD237 pKa = 2.81 IKK239 pKa = 10.1 YY240 pKa = 10.05 AYY242 pKa = 9.89 NGEE245 pKa = 4.22 RR246 pKa = 11.84 IVIFDD251 pKa = 4.18 LSRR254 pKa = 11.84 SQEE257 pKa = 3.57 DD258 pKa = 3.51 HH259 pKa = 6.69 FNYY262 pKa = 10.13 EE263 pKa = 3.75 VLEE266 pKa = 4.2 SVKK269 pKa = 10.71 NGIMFSPKK277 pKa = 9.25 FDD279 pKa = 3.52 SKK281 pKa = 10.73 MKK283 pKa = 10.23 VFAVPHH289 pKa = 5.3 VLVFANWGPEE299 pKa = 3.78 EE300 pKa = 4.47 SKK302 pKa = 10.74 LSRR305 pKa = 11.84 DD306 pKa = 3.26 RR307 pKa = 11.84 WDD309 pKa = 3.69 VKK311 pKa = 11.18 CMTDD315 pKa = 5.27 QDD317 pKa = 4.5 MEE319 pKa = 4.27 WSDD322 pKa = 3.55 LASNVIVKK330 pKa = 9.96 QEE332 pKa = 3.87 GHH334 pKa = 5.93 NVLIDD339 pKa = 3.39 MTGDD343 pKa = 3.45 RR344 pKa = 11.84 EE345 pKa = 4.44 ADD347 pKa = 3.69 TQVMDD352 pKa = 4.06 WNSTDD357 pKa = 5.34 DD358 pKa = 3.68 SDD360 pKa = 5.46 CFIVQYY366 pKa = 11.25
Molecular weight: 41.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.536
IPC2_protein 5.486
IPC_protein 5.499
Toseland 5.792
ProMoST 5.792
Dawson 5.677
Bjellqvist 5.715
Wikipedia 5.652
Rodwell 5.639
Grimsley 5.906
Solomon 5.677
Lehninger 5.652
Nozaki 5.906
DTASelect 6.084
Thurlkill 6.02
EMBOSS 5.982
Sillero 5.995
Patrickios 4.228
IPC_peptide 5.69
IPC2_peptide 5.995
IPC2.peptide.svr19 5.992
Protein with the highest isoelectric point:
>tr|A0A0K1RLM4|A0A0K1RLM4_9CIRC Putative replication initiation protein OS=Littorina sp. associated circular virus OX=1692253 PE=4 SV=1
MM1 pKa = 8.11 AKK3 pKa = 10.23 RR4 pKa = 11.84 KK5 pKa = 5.39 MTSYY9 pKa = 10.53 FKK11 pKa = 10.5 SKK13 pKa = 10.08 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 FTAPSRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 GPFGGGNKK33 pKa = 7.94 TSRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 FTRR42 pKa = 11.84 TRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 TRR50 pKa = 11.84 NQPSRR55 pKa = 11.84 YY56 pKa = 7.72 QVFNARR62 pKa = 11.84 IYY64 pKa = 8.68 YY65 pKa = 8.57 TVAADD70 pKa = 5.08 ANGQLYY76 pKa = 9.76 RR77 pKa = 11.84 NNYY80 pKa = 9.39 LSVHH84 pKa = 5.91 GAIQEE89 pKa = 4.01 IQNNNRR95 pKa = 11.84 AIYY98 pKa = 10.47 DD99 pKa = 3.78 SVLTNWDD106 pKa = 3.17 KK107 pKa = 11.4 FKK109 pKa = 10.94 FLSKK113 pKa = 9.23 TEE115 pKa = 4.42 RR116 pKa = 11.84 IFMSDD121 pKa = 2.89 TSPYY125 pKa = 10.66 NDD127 pKa = 3.55 TARR130 pKa = 11.84 DD131 pKa = 3.5 RR132 pKa = 11.84 ATTLYY137 pKa = 10.29 HH138 pKa = 6.95 VYY140 pKa = 10.73 DD141 pKa = 4.46 PDD143 pKa = 3.55 SRR145 pKa = 11.84 GRR147 pKa = 11.84 RR148 pKa = 11.84 MAPSEE153 pKa = 4.15 MMKK156 pKa = 9.75 MPACHH161 pKa = 6.39 WKK163 pKa = 10.34 FIRR166 pKa = 11.84 PYY168 pKa = 9.94 QVYY171 pKa = 10.04 KK172 pKa = 9.85 STLRR176 pKa = 11.84 PVFQIPAAGAVLDD189 pKa = 4.32 GGEE192 pKa = 4.14 ISTGIPRR199 pKa = 11.84 VDD201 pKa = 3.93 NPWRR205 pKa = 11.84 DD206 pKa = 3.61 CANFTPTGLASLEE219 pKa = 4.22 SHH221 pKa = 6.4 NGAMYY226 pKa = 10.46 SISTSPNQQFTVIRR240 pKa = 11.84 TYY242 pKa = 10.71 KK243 pKa = 10.2 IAVTTPRR250 pKa = 11.84 YY251 pKa = 8.1 GQLYY255 pKa = 9.52 QGQDD259 pKa = 2.79 NTNYY263 pKa = 9.99 IEE265 pKa = 3.94
Molecular weight: 30.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 10.087
IPC_protein 10.994
Toseland 10.774
ProMoST 10.687
Dawson 10.906
Bjellqvist 10.716
Wikipedia 11.199
Rodwell 10.979
Grimsley 10.994
Solomon 11.067
Lehninger 11.008
Nozaki 10.76
DTASelect 10.716
Thurlkill 10.804
EMBOSS 11.199
Sillero 10.847
Patrickios 10.613
IPC_peptide 11.067
IPC2_peptide 9.838
IPC2.peptide.svr19 8.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
631
265
366
315.5
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.607 ± 0.27
1.426 ± 0.415
6.339 ± 1.12
5.23 ± 1.834
3.645 ± 0.546
7.607 ± 1.204
2.377 ± 0.537
4.596 ± 0.192
6.339 ± 1.12
5.705 ± 1.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.328 ± 0.191
4.754 ± 1.027
3.962 ± 1.05
4.12 ± 0.252
8.399 ± 2.974
6.181 ± 0.612
5.705 ± 1.839
6.339 ± 1.353
1.902 ± 0.476
4.437 ± 1.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here