Gemmiger sp. An194

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Gemmiger; unclassified Gemmiger

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2772 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4IY31|A0A1Y4IY31_9FIRM Segregation and condensation protein B OS=Gemmiger sp. An194 OX=1965582 GN=scpB PE=3 SV=1
MM1 pKa = 7.3NKK3 pKa = 10.14KK4 pKa = 10.35NILTAAVSLSLVACLSIGATLAYY27 pKa = 8.7FTDD30 pKa = 3.63KK31 pKa = 11.25TDD33 pKa = 3.32VKK35 pKa = 11.22NNAFTTGNVDD45 pKa = 2.73ITLTDD50 pKa = 3.24TSDD53 pKa = 3.22SDD55 pKa = 3.46KK56 pKa = 10.77ATEE59 pKa = 4.0VEE61 pKa = 4.15TGIVYY66 pKa = 10.67DD67 pKa = 5.13DD68 pKa = 4.34VLPGDD73 pKa = 4.16NLDD76 pKa = 3.52KK77 pKa = 11.07NVYY80 pKa = 8.3VTVDD84 pKa = 3.39KK85 pKa = 10.96DD86 pKa = 3.39SSAAYY91 pKa = 9.4VGVFVSVDD99 pKa = 3.4YY100 pKa = 11.52NHH102 pKa = 6.9FGRR105 pKa = 11.84PDD107 pKa = 3.31SYY109 pKa = 11.9DD110 pKa = 3.46VMGLVYY116 pKa = 10.58DD117 pKa = 3.69AMMRR121 pKa = 11.84QGTMNNWNEE130 pKa = 4.02YY131 pKa = 7.61YY132 pKa = 11.22VNVGSTDD139 pKa = 3.22GVLYY143 pKa = 10.56VYY145 pKa = 10.55NQVVDD150 pKa = 3.82PDD152 pKa = 3.68GTEE155 pKa = 4.49DD156 pKa = 3.59VVLDD160 pKa = 4.66LFSDD164 pKa = 3.8IQIPTTWDD172 pKa = 3.11NNFAGAQFGINVQAFATQAEE192 pKa = 4.58NFSEE196 pKa = 4.45GQFIEE201 pKa = 4.51MVDD204 pKa = 3.7GTLTDD209 pKa = 3.51ANGNVIEE216 pKa = 4.46FQEE219 pKa = 4.53FGNKK223 pKa = 8.3

Molecular weight: 24.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4IRX0|A0A1Y4IRX0_9FIRM Chromosome partitioning protein ParB OS=Gemmiger sp. An194 OX=1965582 GN=B5F28_13170 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.37KK9 pKa = 7.56RR10 pKa = 11.84QRR12 pKa = 11.84SGVHH16 pKa = 5.7GFLKK20 pKa = 10.63RR21 pKa = 11.84MSTKK25 pKa = 10.4NGRR28 pKa = 11.84KK29 pKa = 9.32VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2772

0

2772

896356

37

3108

323.4

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.328 ± 0.062

1.705 ± 0.021

5.148 ± 0.04

6.695 ± 0.048

3.863 ± 0.029

7.934 ± 0.047

1.717 ± 0.019

5.136 ± 0.036

4.576 ± 0.041

10.336 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.024

3.356 ± 0.029

4.326 ± 0.036

4.115 ± 0.027

5.413 ± 0.043

5.365 ± 0.033

5.377 ± 0.04

7.211 ± 0.037

1.203 ± 0.021

3.428 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski