Nepavirus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7LKW0|J7LKW0_9VIRU Capsid OS=Nepavirus OX=1224510 PE=4 SV=2
MM1 pKa = 7.55TFRR4 pKa = 11.84LQGRR8 pKa = 11.84HH9 pKa = 4.77IAITYY14 pKa = 7.65SRR16 pKa = 11.84CDD18 pKa = 3.41YY19 pKa = 11.5ALDD22 pKa = 5.77DD23 pKa = 4.12ILAFLKK29 pKa = 10.61NKK31 pKa = 10.02HH32 pKa = 6.01SGSRR36 pKa = 11.84TVQHH40 pKa = 6.52VIVCSEE46 pKa = 3.86THH48 pKa = 5.78QDD50 pKa = 3.41GSFHH54 pKa = 5.89RR55 pKa = 11.84HH56 pKa = 4.49AYY58 pKa = 8.76VRR60 pKa = 11.84YY61 pKa = 8.3SGKK64 pKa = 10.63VNITNPEE71 pKa = 3.95YY72 pKa = 10.69FKK74 pKa = 10.92FRR76 pKa = 11.84HH77 pKa = 5.37AQCNIQPCRR86 pKa = 11.84NVAAWNNYY94 pKa = 5.76VRR96 pKa = 11.84KK97 pKa = 10.58DD98 pKa = 3.14GDD100 pKa = 3.68FVEE103 pKa = 4.56WEE105 pKa = 3.98EE106 pKa = 5.41DD107 pKa = 3.34ATSSNGLYY115 pKa = 10.72EE116 pKa = 4.2NAQNMGEE123 pKa = 3.89ADD125 pKa = 4.28FFQWALRR132 pKa = 11.84SKK134 pKa = 10.25IQFGYY139 pKa = 9.93AQRR142 pKa = 11.84AWDD145 pKa = 4.02TQKK148 pKa = 11.14KK149 pKa = 8.14AAKK152 pKa = 10.08LITFDD157 pKa = 3.76EE158 pKa = 4.95DD159 pKa = 3.57NNPFLDD165 pKa = 4.11LNLPLTQEE173 pKa = 3.9LAGWHH178 pKa = 6.39LSTNLTNVLVGPTGCGKK195 pKa = 8.28TVYY198 pKa = 10.3CFRR201 pKa = 11.84NLLKK205 pKa = 10.72PMLLISHH212 pKa = 7.19IDD214 pKa = 3.62DD215 pKa = 4.79LKK217 pKa = 11.35HH218 pKa = 6.6FDD220 pKa = 4.23PNLHH224 pKa = 6.5RR225 pKa = 11.84SILFDD230 pKa = 5.3DD231 pKa = 4.23MKK233 pKa = 10.87FDD235 pKa = 3.39QWPIQAQIHH244 pKa = 5.73LCDD247 pKa = 3.82RR248 pKa = 11.84GLPRR252 pKa = 11.84SIHH255 pKa = 5.57RR256 pKa = 11.84RR257 pKa = 11.84YY258 pKa = 9.02GTTLIPAGIQIAITCNEE275 pKa = 3.98NSFCLPP281 pKa = 3.46

Molecular weight: 32.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9T6I9|J9T6I9_9VIRU Uncharacterized protein OS=Nepavirus OX=1224510 PE=4 SV=1
MM1 pKa = 7.51SVPTVLWLVGLVLVLLDD18 pKa = 3.58WVLTNLIVLVLSLALEE34 pKa = 4.04VLLYY38 pKa = 10.8GVLLLVFLLLPRR50 pKa = 11.84EE51 pKa = 4.08HH52 pKa = 7.32LLVVLLVLLGVMPYY66 pKa = 11.08AKK68 pKa = 10.4VFVRR72 pKa = 11.84WLVAFLLSGRR82 pKa = 11.84RR83 pKa = 11.84LRR85 pKa = 11.84LPTLSAALL93 pKa = 3.7

Molecular weight: 10.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

863

93

489

287.7

32.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.111 ± 1.034

1.622 ± 0.703

4.751 ± 1.032

3.36 ± 0.265

4.171 ± 0.666

8.227 ± 1.863

2.317 ± 1.013

5.33 ± 0.946

4.519 ± 0.786

10.545 ± 5.629

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.738 ± 0.145

5.33 ± 0.988

4.635 ± 0.369

3.94 ± 0.927

7.3 ± 0.845

6.489 ± 0.996

4.519 ± 0.527

7.879 ± 2.683

1.622 ± 0.506

3.592 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski