Alstroemeria virus X
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q3V6G7|Q3V6G7_9VIRU ORF1 protein OS=Alstroemeria virus X OX=316983 PE=4 SV=1
MM1 pKa = 7.64 ALFYY5 pKa = 11.0 LQTLLLNSDD14 pKa = 4.57 FTRR17 pKa = 11.84 TTTPTASPLIIFGTAGCGKK36 pKa = 8.9 TSIIRR41 pKa = 11.84 ALASQFPEE49 pKa = 3.7 LHH51 pKa = 6.33 FSSFHH56 pKa = 6.87 PIVLLPNIRR65 pKa = 11.84 KK66 pKa = 8.71 KK67 pKa = 10.19 QHH69 pKa = 5.97 LANPNEE75 pKa = 4.16 ATDD78 pKa = 3.66 VLDD81 pKa = 4.47 EE82 pKa = 4.71 FLAGPNPEE90 pKa = 3.49 VRR92 pKa = 11.84 IAKK95 pKa = 9.94 FCDD98 pKa = 3.2 PLQYY102 pKa = 10.87 NCEE105 pKa = 4.31 TLPEE109 pKa = 3.94 PHH111 pKa = 7.03 FISEE115 pKa = 4.03 TTYY118 pKa = 10.06 RR119 pKa = 11.84 FCPRR123 pKa = 11.84 TCEE126 pKa = 3.95 LLNDD130 pKa = 3.79 IFKK133 pKa = 8.39 TTLKK137 pKa = 10.78 SKK139 pKa = 10.5 VLEE142 pKa = 4.19 ICKK145 pKa = 9.52 VACVDD150 pKa = 4.47 PYY152 pKa = 11.71 AVDD155 pKa = 3.45 PVGKK159 pKa = 10.33 VIAIEE164 pKa = 4.06 QEE166 pKa = 4.01 LFPILSAHH174 pKa = 6.47 GLTVHH179 pKa = 6.64 SPDD182 pKa = 3.81 FLTGQTIPEE191 pKa = 4.07 VSVYY195 pKa = 9.69 TLNLKK200 pKa = 10.24 RR201 pKa = 11.84 AVAEE205 pKa = 4.23 HH206 pKa = 6.27 PHH208 pKa = 7.14 LLFIALTRR216 pKa = 11.84 HH217 pKa = 5.82 SKK219 pKa = 8.82 TLHH222 pKa = 6.62 LFDD225 pKa = 6.16 LNAGPDD231 pKa = 3.5 TTAA234 pKa = 3.97
Molecular weight: 26.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.827
IPC2_protein 5.804
IPC_protein 5.881
Toseland 6.275
ProMoST 6.275
Dawson 6.198
Bjellqvist 6.186
Wikipedia 6.224
Rodwell 6.198
Grimsley 6.415
Solomon 6.198
Lehninger 6.198
Nozaki 6.478
DTASelect 6.664
Thurlkill 6.693
EMBOSS 6.664
Sillero 6.59
Patrickios 3.897
IPC_peptide 6.224
IPC2_peptide 6.576
IPC2.peptide.svr19 6.493
Protein with the highest isoelectric point:
>tr|Q3V6G6|Q3V6G6_9VIRU Triple gene block protein 1 OS=Alstroemeria virus X OX=316983 PE=4 SV=1
MM1 pKa = 7.83 PGLTPPPNYY10 pKa = 10.02 EE11 pKa = 3.74 NVYY14 pKa = 10.76 KK15 pKa = 10.71 LVALGFICVGIILATKK31 pKa = 10.14 SNHH34 pKa = 4.55 NHH36 pKa = 5.6 HH37 pKa = 6.96 TGDD40 pKa = 3.72 QQHH43 pKa = 6.38 SLPFGGIYY51 pKa = 9.64 RR52 pKa = 11.84 DD53 pKa = 3.6 GTKK56 pKa = 9.12 TVSYY60 pKa = 9.19 FRR62 pKa = 11.84 PSDD65 pKa = 4.67 PITHH69 pKa = 5.83 SNKK72 pKa = 8.66 WLAFSSVITISLIIWLCSKK91 pKa = 9.82 FSPGDD96 pKa = 3.55 RR97 pKa = 11.84 RR98 pKa = 11.84 PLPPVCSHH106 pKa = 6.29 CTSHH110 pKa = 7.22
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.335
IPC2_protein 8.17
IPC_protein 8.2
Toseland 8.097
ProMoST 8.536
Dawson 8.756
Bjellqvist 9.048
Wikipedia 8.712
Rodwell 8.77
Grimsley 7.892
Solomon 9.004
Lehninger 9.004
Nozaki 9.253
DTASelect 8.785
Thurlkill 8.843
EMBOSS 8.96
Sillero 9.151
Patrickios 3.897
IPC_peptide 9.004
IPC2_peptide 8.258
IPC2.peptide.svr19 8.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2319
99
1646
463.8
51.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.107 ± 1.344
1.682 ± 0.389
5.175 ± 0.648
5.175 ± 0.73
4.873 ± 0.304
4.312 ± 0.713
3.493 ± 0.65
5.218 ± 0.448
5.908 ± 0.957
8.969 ± 1.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.156 ± 0.465
4.873 ± 0.23
7.288 ± 0.668
4.269 ± 0.856
4.01 ± 0.316
6.684 ± 0.795
8.064 ± 0.372
5.52 ± 0.266
1.035 ± 0.212
3.191 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here