Propionibacterium phage Doucette

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Doucettevirus; Propionibacterium virus Doucette

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8ETP5|A0A1D8ETP5_9CAUD Uncharacterized protein OS=Propionibacterium phage Doucette OX=1897534 GN=11 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 5.32RR3 pKa = 11.84GIDD6 pKa = 3.56VSSYY10 pKa = 8.85QAPDD14 pKa = 3.14GGLLEE19 pKa = 5.1GMSFCIIKK27 pKa = 9.05ATEE30 pKa = 3.75GTGYY34 pKa = 10.82VNPNRR39 pKa = 11.84AQWYY43 pKa = 9.42KK44 pKa = 9.72VATDD48 pKa = 3.46RR49 pKa = 11.84GVTTGAYY56 pKa = 9.68HH57 pKa = 6.78FMRR60 pKa = 11.84GGDD63 pKa = 3.55PAQQADD69 pKa = 4.29YY70 pKa = 10.82FLANLPGDD78 pKa = 4.31VDD80 pKa = 3.67WLALDD85 pKa = 4.4VEE87 pKa = 4.65DD88 pKa = 5.9AGDD91 pKa = 3.88GLSWAGRR98 pKa = 11.84VGFILAWTDD107 pKa = 3.06RR108 pKa = 11.84AKK110 pKa = 10.59ATGKK114 pKa = 7.61PTLVYY119 pKa = 10.6ASRR122 pKa = 11.84SWANALWGAASSDD135 pKa = 3.05EE136 pKa = 4.25RR137 pKa = 11.84ARR139 pKa = 11.84LAQLPLWVADD149 pKa = 3.52YY150 pKa = 9.55TRR152 pKa = 11.84RR153 pKa = 11.84PGEE156 pKa = 3.98YY157 pKa = 9.59SGPWPGGWPIAMHH170 pKa = 6.68QYY172 pKa = 9.81TSAPIDD178 pKa = 3.78KK179 pKa = 10.49NVLLTDD185 pKa = 4.42DD186 pKa = 4.48LQGGDD191 pKa = 5.05DD192 pKa = 4.5MPLSTDD198 pKa = 3.44DD199 pKa = 4.31LRR201 pKa = 11.84NIATAVWGGGGAASPMYY218 pKa = 10.7ADD220 pKa = 4.8RR221 pKa = 11.84LDD223 pKa = 3.53GHH225 pKa = 5.93QEE227 pKa = 3.94YY228 pKa = 10.32PEE230 pKa = 4.06TALFSLQQRR239 pKa = 11.84LGARR243 pKa = 11.84FGEE246 pKa = 4.22LSGKK250 pKa = 9.81IDD252 pKa = 3.76GLAEE256 pKa = 4.07ALKK259 pKa = 10.24QVQGGNGVDD268 pKa = 3.38ADD270 pKa = 4.8AIRR273 pKa = 11.84AAAAEE278 pKa = 4.25GAKK281 pKa = 10.52AGVQAMIDD289 pKa = 3.53TATVNLEE296 pKa = 3.98AKK298 pKa = 10.19

Molecular weight: 31.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8ETR1|A0A1D8ETR1_9CAUD Uncharacterized protein OS=Propionibacterium phage Doucette OX=1897534 GN=28 PE=4 SV=1
MM1 pKa = 7.79SLFQLLATHH10 pKa = 6.32WEE12 pKa = 4.04EE13 pKa = 5.44LEE15 pKa = 4.77GDD17 pKa = 4.1FQQTYY22 pKa = 10.76GIDD25 pKa = 4.89LRR27 pKa = 11.84DD28 pKa = 3.37LWRR31 pKa = 11.84GRR33 pKa = 11.84LSAARR38 pKa = 11.84CWVLLTQLPPGSRR51 pKa = 11.84IWRR54 pKa = 11.84MLGGPMAWGMVEE66 pKa = 3.67RR67 pKa = 11.84AVRR70 pKa = 11.84EE71 pKa = 4.16EE72 pKa = 3.9GWRR75 pKa = 11.84LASQNAGKK83 pKa = 9.78EE84 pKa = 4.22LPRR87 pKa = 11.84PEE89 pKa = 4.53PPAPGWRR96 pKa = 11.84DD97 pKa = 3.31KK98 pKa = 11.28QDD100 pKa = 3.33DD101 pKa = 3.47LRR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84EE106 pKa = 3.92EE107 pKa = 3.63RR108 pKa = 11.84RR109 pKa = 11.84LARR112 pKa = 11.84FMQRR116 pKa = 11.84HH117 pKa = 5.66AEE119 pKa = 4.09RR120 pKa = 11.84NNN122 pKa = 3.13

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11468

27

1364

188.0

20.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.836 ± 0.417

0.846 ± 0.138

6.488 ± 0.351

5.049 ± 0.434

2.721 ± 0.214

8.685 ± 0.503

1.727 ± 0.197

4.63 ± 0.402

3.523 ± 0.232

8.371 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.118

1.849 ± 0.181

5.956 ± 0.343

3.907 ± 0.186

6.802 ± 0.628

6.2 ± 0.352

6.767 ± 0.39

7.412 ± 0.438

2.546 ± 0.207

1.299 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski