Amino acid dipepetide frequency for Lachnoclostridium sp. An181

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.204AlaAla: 7.204 ± 0.145	0.972AlaCys: 0.972 ± 0.038	4.666AlaAsp: 4.666 ± 0.107	5.035AlaGlu: 5.035 ± 0.094	2.981AlaPhe: 2.981 ± 0.071	6.057AlaGly: 6.057 ± 0.091	1.07AlaHis: 1.07 ± 0.041	4.586AlaIle: 4.586 ± 0.079	5.984AlaLys: 5.984 ± 0.099	6.737AlaLeu: 6.737 ± 0.099	2.434AlaMet: 2.434 ± 0.051	2.744AlaAsn: 2.744 ± 0.07	1.908AlaPro: 1.908 ± 0.053	2.298AlaGln: 2.298 ± 0.056	2.722AlaArg: 2.722 ± 0.06	3.666AlaSer: 3.666 ± 0.087	3.077AlaThr: 3.077 ± 0.079	6.448AlaVal: 6.448 ± 0.107	0.764AlaTrp: 0.764 ± 0.032	2.683AlaTyr: 2.683 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.999CysAla: 0.999 ± 0.043	0.223CysCys: 0.223 ± 0.02	0.713CysAsp: 0.713 ± 0.031	0.861CysGlu: 0.861 ± 0.033	0.562CysPhe: 0.562 ± 0.027	1.279CysGly: 1.279 ± 0.045	0.298CysHis: 0.298 ± 0.019	1.029CysIle: 1.029 ± 0.043	0.764CysLys: 0.764 ± 0.03	1.061CysLeu: 1.061 ± 0.043	0.445CysMet: 0.445 ± 0.025	0.483CysAsn: 0.483 ± 0.025	0.586CysPro: 0.586 ± 0.033	0.404CysGln: 0.404 ± 0.023	0.613CysArg: 0.613 ± 0.029	0.722CysSer: 0.722 ± 0.031	0.685CysThr: 0.685 ± 0.03	0.932CysVal: 0.932 ± 0.035	0.1CysTrp: 0.1 ± 0.011	0.492CysTyr: 0.492 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.534AspAla: 4.534 ± 0.103	0.72AspCys: 0.72 ± 0.035	2.85AspAsp: 2.85 ± 0.06	5.169AspGlu: 5.169 ± 0.087	2.504AspPhe: 2.504 ± 0.057	4.856AspGly: 4.856 ± 0.125	0.784AspHis: 0.784 ± 0.032	4.298AspIle: 4.298 ± 0.082	4.004AspLys: 4.004 ± 0.081	4.524AspLeu: 4.524 ± 0.081	1.897AspMet: 1.897 ± 0.05	2.341AspAsn: 2.341 ± 0.07	1.467AspPro: 1.467 ± 0.048	1.342AspGln: 1.342 ± 0.05	2.079AspArg: 2.079 ± 0.05	3.046AspSer: 3.046 ± 0.074	3.301AspThr: 3.301 ± 0.08	4.094AspVal: 4.094 ± 0.077	0.593AspTrp: 0.593 ± 0.027	2.707AspTyr: 2.707 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	4.677GluAla: 4.677 ± 0.084	0.824GluCys: 0.824 ± 0.033	4.728GluAsp: 4.728 ± 0.085	9.133GluGlu: 9.133 ± 0.149	2.907GluPhe: 2.907 ± 0.058	5.349GluGly: 5.349 ± 0.091	1.4GluHis: 1.4 ± 0.048	5.99GluIle: 5.99 ± 0.084	8.24GluLys: 8.24 ± 0.134	6.788GluLeu: 6.788 ± 0.108	2.649GluMet: 2.649 ± 0.063	4.472GluAsn: 4.472 ± 0.08	1.982GluPro: 1.982 ± 0.053	3.004GluGln: 3.004 ± 0.074	3.596GluArg: 3.596 ± 0.078	3.637GluSer: 3.637 ± 0.072	4.574GluThr: 4.574 ± 0.09	5.084GluVal: 5.084 ± 0.087	0.791GluTrp: 0.791 ± 0.033	3.364GluTyr: 3.364 ± 0.071	0.0GluXaa: 0.0 ± 0.0
Phe	2.949PheAla: 2.949 ± 0.059	0.697PheCys: 0.697 ± 0.029	2.498PheAsp: 2.498 ± 0.052	3.044PheGlu: 3.044 ± 0.068	1.868PhePhe: 1.868 ± 0.055	3.128PheGly: 3.128 ± 0.066	0.894PheHis: 0.894 ± 0.037	2.55PheIle: 2.55 ± 0.067	1.971PheLys: 1.971 ± 0.048	4.174PheLeu: 4.174 ± 0.094	1.028PheMet: 1.028 ± 0.039	1.353PheAsn: 1.353 ± 0.036	1.355PhePro: 1.355 ± 0.043	1.411PheGln: 1.411 ± 0.039	1.773PheArg: 1.773 ± 0.046	2.617PheSer: 2.617 ± 0.06	2.249PheThr: 2.249 ± 0.055	2.96PheVal: 2.96 ± 0.069	0.438PheTrp: 0.438 ± 0.025	1.722PheTyr: 1.722 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.967GlyAla: 4.967 ± 0.097	1.143GlyCys: 1.143 ± 0.04	3.83GlyAsp: 3.83 ± 0.088	5.533GlyGlu: 5.533 ± 0.099	3.037GlyPhe: 3.037 ± 0.063	4.755GlyGly: 4.755 ± 0.1	1.18GlyHis: 1.18 ± 0.041	5.969GlyIle: 5.969 ± 0.11	6.046GlyLys: 6.046 ± 0.086	5.473GlyLeu: 5.473 ± 0.084	2.496GlyMet: 2.496 ± 0.065	3.535GlyAsn: 3.535 ± 0.074	1.24GlyPro: 1.24 ± 0.039	2.429GlyGln: 2.429 ± 0.052	3.095GlyArg: 3.095 ± 0.066	3.74GlySer: 3.74 ± 0.073	4.408GlyThr: 4.408 ± 0.089	5.079GlyVal: 5.079 ± 0.076	0.745GlyTrp: 0.745 ± 0.037	3.194GlyTyr: 3.194 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	1.163HisAla: 1.163 ± 0.039	0.276HisCys: 0.276 ± 0.021	0.798HisAsp: 0.798 ± 0.035	1.068HisGlu: 1.068 ± 0.036	0.773HisPhe: 0.773 ± 0.031	1.233HisGly: 1.233 ± 0.042	0.364HisHis: 0.364 ± 0.03	1.373HisIle: 1.373 ± 0.049	0.877HisLys: 0.877 ± 0.036	1.448HisLeu: 1.448 ± 0.045	0.543HisMet: 0.543 ± 0.025	0.635HisAsn: 0.635 ± 0.027	0.792HisPro: 0.792 ± 0.031	0.49HisGln: 0.49 ± 0.023	0.724HisArg: 0.724 ± 0.034	0.945HisSer: 0.945 ± 0.034	0.995HisThr: 0.995 ± 0.032	1.205HisVal: 1.205 ± 0.033	0.163HisTrp: 0.163 ± 0.015	0.75HisTyr: 0.75 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.349IleAla: 5.349 ± 0.093	1.129IleCys: 1.129 ± 0.039	3.798IleAsp: 3.798 ± 0.074	4.849IleGlu: 4.849 ± 0.083	2.884IlePhe: 2.884 ± 0.073	5.062IleGly: 5.062 ± 0.09	1.301IleHis: 1.301 ± 0.04	4.34IleIle: 4.34 ± 0.098	4.236IleLys: 4.236 ± 0.083	6.903IleLeu: 6.903 ± 0.129	1.818IleMet: 1.818 ± 0.049	2.547IleAsn: 2.547 ± 0.058	3.087IlePro: 3.087 ± 0.069	2.414IleGln: 2.414 ± 0.056	3.377IleArg: 3.377 ± 0.082	4.609IleSer: 4.609 ± 0.092	3.817IleThr: 3.817 ± 0.074	5.035IleVal: 5.035 ± 0.101	0.67IleTrp: 0.67 ± 0.027	2.763IleTyr: 2.763 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	5.676LysAla: 5.676 ± 0.097	0.646LysCys: 0.646 ± 0.032	4.66LysAsp: 4.66 ± 0.087	8.875LysGlu: 8.875 ± 0.132	2.027LysPhe: 2.027 ± 0.05	4.803LysGly: 4.803 ± 0.076	0.956LysHis: 0.956 ± 0.04	5.205LysIle: 5.205 ± 0.09	7.174LysLys: 7.174 ± 0.136	5.536LysLeu: 5.536 ± 0.101	2.423LysMet: 2.423 ± 0.057	3.698LysAsn: 3.698 ± 0.073	2.033LysPro: 2.033 ± 0.049	2.588LysGln: 2.588 ± 0.062	3.579LysArg: 3.579 ± 0.08	3.498LysSer: 3.498 ± 0.071	4.268LysThr: 4.268 ± 0.076	4.908LysVal: 4.908 ± 0.079	0.655LysTrp: 0.655 ± 0.03	2.885LysTyr: 2.885 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	6.809LeuAla: 6.809 ± 0.094	1.355LeuCys: 1.355 ± 0.048	5.147LeuAsp: 5.147 ± 0.076	7.117LeuGlu: 7.117 ± 0.096	3.628LeuPhe: 3.628 ± 0.092	5.764LeuGly: 5.764 ± 0.11	1.465LeuHis: 1.465 ± 0.042	5.578LeuIle: 5.578 ± 0.105	6.858LeuLys: 6.858 ± 0.104	7.925LeuLeu: 7.925 ± 0.162	2.335LeuMet: 2.335 ± 0.056	3.622LeuAsn: 3.622 ± 0.072	3.198LeuPro: 3.198 ± 0.073	2.554LeuGln: 2.554 ± 0.058	3.38LeuArg: 3.38 ± 0.069	5.951LeuSer: 5.951 ± 0.106	4.709LeuThr: 4.709 ± 0.09	5.323LeuVal: 5.323 ± 0.097	0.724LeuTrp: 0.724 ± 0.035	3.369LeuTyr: 3.369 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	2.381MetAla: 2.381 ± 0.057	0.347MetCys: 0.347 ± 0.023	1.92MetAsp: 1.92 ± 0.045	2.907MetGlu: 2.907 ± 0.064	1.033MetPhe: 1.033 ± 0.041	1.975MetGly: 1.975 ± 0.054	0.473MetHis: 0.473 ± 0.022	2.017MetIle: 2.017 ± 0.059	2.706MetLys: 2.706 ± 0.062	2.536MetLeu: 2.536 ± 0.062	0.994MetMet: 0.994 ± 0.035	1.463MetAsn: 1.463 ± 0.044	1.059MetPro: 1.059 ± 0.04	1.237MetGln: 1.237 ± 0.042	1.324MetArg: 1.324 ± 0.043	1.651MetSer: 1.651 ± 0.046	1.61MetThr: 1.61 ± 0.049	1.809MetVal: 1.809 ± 0.049	0.251MetTrp: 0.251 ± 0.022	0.945MetTyr: 0.945 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.305AsnAla: 3.305 ± 0.092	0.526AsnCys: 0.526 ± 0.026	2.025AsnAsp: 2.025 ± 0.063	3.131AsnGlu: 3.131 ± 0.075	1.561AsnPhe: 1.561 ± 0.043	3.738AsnGly: 3.738 ± 0.105	0.787AsnHis: 0.787 ± 0.03	3.317AsnIle: 3.317 ± 0.064	2.537AsnLys: 2.537 ± 0.058	3.857AsnLeu: 3.857 ± 0.069	1.349AsnMet: 1.349 ± 0.043	1.731AsnAsn: 1.731 ± 0.057	1.926AsnPro: 1.926 ± 0.046	1.441AsnGln: 1.441 ± 0.048	1.895AsnArg: 1.895 ± 0.054	2.184AsnSer: 2.184 ± 0.062	2.41AsnThr: 2.41 ± 0.064	3.063AsnVal: 3.063 ± 0.076	0.436AsnTrp: 0.436 ± 0.024	1.758AsnTyr: 1.758 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	2.228ProAla: 2.228 ± 0.059	0.421ProCys: 0.421 ± 0.023	2.213ProAsp: 2.213 ± 0.052	2.779ProGlu: 2.779 ± 0.059	1.47ProPhe: 1.47 ± 0.044	2.233ProGly: 2.233 ± 0.056	0.562ProHis: 0.562 ± 0.022	1.965ProIle: 1.965 ± 0.06	2.295ProLys: 2.295 ± 0.05	2.5ProLeu: 2.5 ± 0.063	0.836ProMet: 0.836 ± 0.034	1.262ProAsn: 1.262 ± 0.043	0.655ProPro: 0.655 ± 0.031	0.961ProGln: 0.961 ± 0.037	0.851ProArg: 0.851 ± 0.033	1.817ProSer: 1.817 ± 0.055	1.424ProThr: 1.424 ± 0.043	2.868ProVal: 2.868 ± 0.062	0.307ProTrp: 0.307 ± 0.018	1.469ProTyr: 1.469 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.438GlnAla: 2.438 ± 0.062	0.31GlnCys: 0.31 ± 0.019	1.689GlnAsp: 1.689 ± 0.051	2.94GlnGlu: 2.94 ± 0.068	1.196GlnPhe: 1.196 ± 0.042	2.205GlnGly: 2.205 ± 0.058	0.414GlnHis: 0.414 ± 0.023	2.53GlnIle: 2.53 ± 0.06	3.144GlnLys: 3.144 ± 0.065	2.487GlnLeu: 2.487 ± 0.06	1.222GlnMet: 1.222 ± 0.039	1.719GlnAsn: 1.719 ± 0.046	0.812GlnPro: 0.812 ± 0.03	0.986GlnGln: 0.986 ± 0.037	1.258GlnArg: 1.258 ± 0.04	1.673GlnSer: 1.673 ± 0.045	1.77GlnThr: 1.77 ± 0.047	2.152GlnVal: 2.152 ± 0.061	0.357GlnTrp: 0.357 ± 0.026	1.466GlnTyr: 1.466 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.598ArgAla: 2.598 ± 0.062	0.464ArgCys: 0.464 ± 0.025	1.976ArgAsp: 1.976 ± 0.052	3.785ArgGlu: 3.785 ± 0.083	1.848ArgPhe: 1.848 ± 0.05	2.43ArgGly: 2.43 ± 0.062	0.751ArgHis: 0.751 ± 0.035	3.345ArgIle: 3.345 ± 0.064	3.666ArgLys: 3.666 ± 0.076	3.653ArgLeu: 3.653 ± 0.074	1.578ArgMet: 1.578 ± 0.043	1.892ArgAsn: 1.892 ± 0.051	1.196ArgPro: 1.196 ± 0.04	1.635ArgGln: 1.635 ± 0.051	2.016ArgArg: 2.016 ± 0.062	1.708ArgSer: 1.708 ± 0.045	2.365ArgThr: 2.365 ± 0.055	2.559ArgVal: 2.559 ± 0.056	0.409ArgTrp: 0.409 ± 0.021	1.744ArgTyr: 1.744 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	4.344SerAla: 4.344 ± 0.088	0.758SerCys: 0.758 ± 0.031	2.957SerAsp: 2.957 ± 0.064	3.726SerGlu: 3.726 ± 0.074	2.693SerPhe: 2.693 ± 0.064	4.792SerGly: 4.792 ± 0.087	0.914SerHis: 0.914 ± 0.032	3.824SerIle: 3.824 ± 0.071	3.324SerLys: 3.324 ± 0.065	4.888SerLeu: 4.888 ± 0.085	1.755SerMet: 1.755 ± 0.046	2.055SerAsn: 2.055 ± 0.053	1.649SerPro: 1.649 ± 0.047	1.761SerGln: 1.761 ± 0.053	2.343SerArg: 2.343 ± 0.055	3.368SerSer: 3.368 ± 0.071	2.764SerThr: 2.764 ± 0.07	4.474SerVal: 4.474 ± 0.084	0.694SerTrp: 0.694 ± 0.03	2.354SerTyr: 2.354 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	4.422ThrAla: 4.422 ± 0.091	0.597ThrCys: 0.597 ± 0.03	3.317ThrAsp: 3.317 ± 0.08	3.776ThrGlu: 3.776 ± 0.077	2.314ThrPhe: 2.314 ± 0.054	4.494ThrGly: 4.494 ± 0.071	0.851ThrHis: 0.851 ± 0.029	3.748ThrIle: 3.748 ± 0.069	3.738ThrLys: 3.738 ± 0.059	4.922ThrLeu: 4.922 ± 0.078	1.439ThrMet: 1.439 ± 0.043	2.182ThrAsn: 2.182 ± 0.05	2.06ThrPro: 2.06 ± 0.05	1.554ThrGln: 1.554 ± 0.046	1.767ThrArg: 1.767 ± 0.048	3.064ThrSer: 3.064 ± 0.067	2.813ThrThr: 2.813 ± 0.074	4.564ThrVal: 4.564 ± 0.09	0.602ThrTrp: 0.602 ± 0.032	2.162ThrTyr: 2.162 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	4.66ValAla: 4.66 ± 0.08	1.11ValCys: 1.11 ± 0.039	4.306ValAsp: 4.306 ± 0.088	5.287ValGlu: 5.287 ± 0.084	3.181ValPhe: 3.181 ± 0.068	4.205ValGly: 4.205 ± 0.086	1.136ValHis: 1.136 ± 0.036	5.02ValIle: 5.02 ± 0.089	5.158ValLys: 5.158 ± 0.078	6.856ValLeu: 6.856 ± 0.11	1.948ValMet: 1.948 ± 0.059	3.043ValAsn: 3.043 ± 0.084	2.384ValPro: 2.384 ± 0.054	2.221ValGln: 2.221 ± 0.051	2.884ValArg: 2.884 ± 0.058	4.826ValSer: 4.826 ± 0.087	4.208ValThr: 4.208 ± 0.086	4.936ValVal: 4.936 ± 0.089	0.64ValTrp: 0.64 ± 0.029	2.778ValTyr: 2.778 ± 0.065	0.0ValXaa: 0.0 ± 0.0
Trp	0.562TrpAla: 0.562 ± 0.029	0.164TrpCys: 0.164 ± 0.014	0.591TrpAsp: 0.591 ± 0.029	0.746TrpGlu: 0.746 ± 0.031	0.417TrpPhe: 0.417 ± 0.027	0.651TrpGly: 0.651 ± 0.032	0.174TrpHis: 0.174 ± 0.016	0.655TrpIle: 0.655 ± 0.029	0.997TrpLys: 0.997 ± 0.037	0.808TrpLeu: 0.808 ± 0.035	0.377TrpMet: 0.377 ± 0.022	0.562TrpAsn: 0.562 ± 0.027	0.193TrpPro: 0.193 ± 0.016	0.447TrpGln: 0.447 ± 0.029	0.419TrpArg: 0.419 ± 0.023	0.539TrpSer: 0.539 ± 0.027	0.475TrpThr: 0.475 ± 0.026	0.568TrpVal: 0.568 ± 0.031	0.1TrpTrp: 0.1 ± 0.012	0.393TrpTyr: 0.393 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.771TyrAla: 2.771 ± 0.054	0.569TyrCys: 0.569 ± 0.025	2.427TyrAsp: 2.427 ± 0.062	3.429TyrGlu: 3.429 ± 0.074	1.797TyrPhe: 1.797 ± 0.049	2.887TyrGly: 2.887 ± 0.065	0.841TyrHis: 0.841 ± 0.035	2.646TyrIle: 2.646 ± 0.063	2.376TyrLys: 2.376 ± 0.059	3.735TyrLeu: 3.735 ± 0.07	1.073TyrMet: 1.073 ± 0.041	1.638TyrAsn: 1.638 ± 0.058	1.532TyrPro: 1.532 ± 0.04	1.59TyrGln: 1.59 ± 0.045	1.925TyrArg: 1.925 ± 0.052	2.173TyrSer: 2.173 ± 0.054	2.462TyrThr: 2.462 ± 0.063	2.724TyrVal: 2.724 ± 0.054	0.402TyrTrp: 0.402 ± 0.021	1.775TyrTyr: 1.775 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2355 proteins (821853 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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