Gopherus associated circular DNA virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384WVX5|A0A384WVX5_9VIRU Replication associated protein OS=Gopherus associated circular DNA virus 1 OX=2041419 PE=3 SV=1
MM1 pKa = 7.27EE2 pKa = 4.18QTGEE6 pKa = 3.94PDD8 pKa = 3.63GNSSSVAQRR17 pKa = 11.84RR18 pKa = 11.84FHH20 pKa = 6.98LNAKK24 pKa = 7.72TLFLTYY30 pKa = 8.75PQCDD34 pKa = 3.78LPRR37 pKa = 11.84EE38 pKa = 4.2EE39 pKa = 5.28ALSLLEE45 pKa = 3.98AAFMTRR51 pKa = 11.84QRR53 pKa = 11.84RR54 pKa = 11.84IIEE57 pKa = 3.85YY58 pKa = 10.19LIASEE63 pKa = 4.15KK64 pKa = 10.38HH65 pKa = 6.19ADD67 pKa = 3.84GTPHH71 pKa = 6.64LHH73 pKa = 6.9CFVKK77 pKa = 10.64LNKK80 pKa = 9.78AVNLKK85 pKa = 10.54RR86 pKa = 11.84PTEE89 pKa = 3.98LDD91 pKa = 3.43LKK93 pKa = 9.64NHH95 pKa = 6.38HH96 pKa = 6.82GNYY99 pKa = 9.05QSCRR103 pKa = 11.84SPEE106 pKa = 3.63AVRR109 pKa = 11.84RR110 pKa = 11.84YY111 pKa = 9.37CSKK114 pKa = 11.03DD115 pKa = 3.11GNFLTNITSGTDD127 pKa = 3.26SQEE130 pKa = 4.29GPWRR134 pKa = 11.84KK135 pKa = 9.14ARR137 pKa = 11.84KK138 pKa = 8.64IVKK141 pKa = 9.94EE142 pKa = 4.09GGDD145 pKa = 3.57LKK147 pKa = 10.99EE148 pKa = 4.43ALLALEE154 pKa = 4.46EE155 pKa = 4.46TEE157 pKa = 3.99KK158 pKa = 10.2TARR161 pKa = 11.84DD162 pKa = 3.37LTIYY166 pKa = 10.73GSTISQALRR175 pKa = 11.84SLKK178 pKa = 10.31PVTEE182 pKa = 4.19LTSEE186 pKa = 4.08QTLTSYY192 pKa = 11.45GPLFQWDD199 pKa = 3.8QAKK202 pKa = 10.52HH203 pKa = 5.39SLLLFGNTEE212 pKa = 4.05TGKK215 pKa = 7.65TTLAKK220 pKa = 10.68LLIPKK225 pKa = 10.04ALFVRR230 pKa = 11.84HH231 pKa = 6.62LDD233 pKa = 3.6LLLSLTEE240 pKa = 3.87EE241 pKa = 4.21HH242 pKa = 6.79GGVILDD248 pKa = 4.31DD249 pKa = 3.46MTFKK253 pKa = 10.69HH254 pKa = 5.97LHH256 pKa = 6.94DD257 pKa = 4.22EE258 pKa = 4.23AQIALLDD265 pKa = 4.22LQDD268 pKa = 3.46EE269 pKa = 4.69THH271 pKa = 5.08IHH273 pKa = 3.89VRR275 pKa = 11.84YY276 pKa = 9.52RR277 pKa = 11.84VAVLPAKK284 pKa = 10.15LPRR287 pKa = 11.84IITSNKK293 pKa = 9.02EE294 pKa = 3.38PWEE297 pKa = 4.22VVNMANPAIARR308 pKa = 11.84RR309 pKa = 11.84VLCVRR314 pKa = 11.84WYY316 pKa = 10.41GWDD319 pKa = 3.37KK320 pKa = 11.03VPMFNVEE327 pKa = 3.67

Molecular weight: 37.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384WVX5|A0A384WVX5_9VIRU Replication associated protein OS=Gopherus associated circular DNA virus 1 OX=2041419 PE=3 SV=1
MM1 pKa = 7.13ARR3 pKa = 11.84SYY5 pKa = 10.76RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84TYY11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.21RR15 pKa = 11.84PTYY18 pKa = 8.83RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84INRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 8.36PMRR30 pKa = 11.84GRR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.43LKK36 pKa = 9.35QRR38 pKa = 11.84RR39 pKa = 11.84FVQRR43 pKa = 11.84NRR45 pKa = 11.84MRR47 pKa = 11.84AQIDD51 pKa = 3.61SNRR54 pKa = 11.84FSNNLGDD61 pKa = 3.62MSYY64 pKa = 7.73TTVRR68 pKa = 11.84DD69 pKa = 3.49THH71 pKa = 5.61WAVMQRR77 pKa = 11.84AVTYY81 pKa = 8.34GTDD84 pKa = 3.09AAGAPFNPKK93 pKa = 10.26AGTDD97 pKa = 3.17NQYY100 pKa = 11.22YY101 pKa = 9.88VGYY104 pKa = 8.96SLPVNYY110 pKa = 9.35FWSRR114 pKa = 11.84ITPGLRR120 pKa = 11.84HH121 pKa = 6.0YY122 pKa = 10.53YY123 pKa = 10.77NNYY126 pKa = 9.69KK127 pKa = 9.95YY128 pKa = 10.27ATVEE132 pKa = 3.78RR133 pKa = 11.84FLIKK137 pKa = 9.99ATFYY141 pKa = 11.24NSGSVLTKK149 pKa = 10.23EE150 pKa = 4.26VGILKK155 pKa = 9.94PDD157 pKa = 3.93AALGWPGINWTPTQLPSEE175 pKa = 4.27QPKK178 pKa = 10.37GVSSFITAHH187 pKa = 6.29PVAGSYY193 pKa = 7.65KK194 pKa = 8.55TLTMALTKK202 pKa = 10.4KK203 pKa = 10.6DD204 pKa = 3.51MYY206 pKa = 10.4QGSRR210 pKa = 11.84LEE212 pKa = 3.99PGRR215 pKa = 11.84GYY217 pKa = 10.78DD218 pKa = 4.64IISTGINGAGLLQPPEE234 pKa = 4.51YY235 pKa = 8.9PTFIYY240 pKa = 9.74IWQGNPIAGVEE251 pKa = 4.09AEE253 pKa = 4.35SFADD257 pKa = 3.81AVWVKK262 pKa = 6.92MTVWRR267 pKa = 11.84RR268 pKa = 11.84VKK270 pKa = 10.58FFGKK274 pKa = 8.84ATPLFAGRR282 pKa = 11.84TDD284 pKa = 3.47EE285 pKa = 4.3ATDD288 pKa = 3.55VYY290 pKa = 9.31TTGQFGTDD298 pKa = 3.27PGDD301 pKa = 3.76EE302 pKa = 3.98VDD304 pKa = 3.65EE305 pKa = 4.9AFTVEE310 pKa = 3.93

Molecular weight: 35.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

637

310

327

318.5

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.535 ± 0.138

0.785 ± 0.524

4.71 ± 0.129

5.651 ± 1.404

3.768 ± 0.5

6.593 ± 1.197

2.512 ± 1.031

4.239 ± 0.03

5.651 ± 0.543

9.262 ± 2.953

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.36

4.239 ± 0.185

5.338 ± 0.528

3.611 ± 0.042

8.477 ± 1.447

5.181 ± 0.228

8.32 ± 0.475

5.651 ± 0.319

1.884 ± 0.25

4.553 ± 1.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski