Capybara microvirus Cap3_SP_445
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8L8|A0A4P8W8L8_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_445 OX=2585457 PE=3 SV=1
MM1 pKa = 7.17 KK2 pKa = 10.3 QNPSNCFKK10 pKa = 10.81 ARR12 pKa = 11.84 SDD14 pKa = 3.33 GKK16 pKa = 10.38 VFPGEE21 pKa = 4.05 VFSPIVKK28 pKa = 9.8 EE29 pKa = 3.86 YY30 pKa = 11.29 SLIDD34 pKa = 3.71 SVIMIGDD41 pKa = 4.04 TPRR44 pKa = 11.84 DD45 pKa = 4.01 FIIHH49 pKa = 5.87 KK50 pKa = 8.89 TPEE53 pKa = 4.91 LINEE57 pKa = 3.88 YY58 pKa = 10.83 DD59 pKa = 3.3 IDD61 pKa = 3.91 KK62 pKa = 11.04 QIAAEE67 pKa = 4.19 AASCDD72 pKa = 3.33 IKK74 pKa = 11.31 SQILAAMQNGSIDD87 pKa = 3.54 SLAWDD92 pKa = 4.9 GSSGAIDD99 pKa = 3.83 ATSMPSNLMEE109 pKa = 4.27 AQNIINAGLNAYY121 pKa = 10.01 DD122 pKa = 5.39 SLDD125 pKa = 3.84 PDD127 pKa = 4.17 LKK129 pKa = 11.11 KK130 pKa = 10.71 KK131 pKa = 10.75 GLDD134 pKa = 3.63 FIKK137 pKa = 11.0 NMTQTEE143 pKa = 4.41 FEE145 pKa = 4.66 SYY147 pKa = 9.75 IKK149 pKa = 10.56 SLIPSQPIEE158 pKa = 4.17 KK159 pKa = 10.56 DD160 pKa = 2.94 GDD162 pKa = 3.77 INGG165 pKa = 3.74
Molecular weight: 18.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.507
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.139
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.228
Rodwell 4.164
Grimsley 4.05
Solomon 4.304
Lehninger 4.266
Nozaki 4.418
DTASelect 4.647
Thurlkill 4.164
EMBOSS 4.24
Sillero 4.444
Patrickios 3.999
IPC_peptide 4.304
IPC2_peptide 4.431
IPC2.peptide.svr19 4.386
Protein with the highest isoelectric point:
>tr|A0A4P8W8L6|A0A4P8W8L6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_445 OX=2585457 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.74 PCLHH6 pKa = 7.04 PSLVVVLGDD15 pKa = 4.54 NPDD18 pKa = 3.47 TGKK21 pKa = 10.86 KK22 pKa = 9.47 IIRR25 pKa = 11.84 FRR27 pKa = 11.84 GRR29 pKa = 11.84 SEE31 pKa = 4.04 DD32 pKa = 4.08 TIQSLSDD39 pKa = 3.16 LYY41 pKa = 11.38 GRR43 pKa = 11.84 EE44 pKa = 3.98 NVLLIPCGQCEE55 pKa = 4.28 SCRR58 pKa = 11.84 INYY61 pKa = 8.16 AQVWSIRR68 pKa = 11.84 CSLEE72 pKa = 4.45 AMMTPYY78 pKa = 10.38 HH79 pKa = 5.96 YY80 pKa = 10.52 FITLTYY86 pKa = 10.87 DD87 pKa = 3.38 DD88 pKa = 4.32 LHH90 pKa = 8.08 YY91 pKa = 10.94 PFANLSDD98 pKa = 3.5 FTKK101 pKa = 9.63 WMKK104 pKa = 10.51 RR105 pKa = 11.84 MRR107 pKa = 11.84 KK108 pKa = 9.28 NYY110 pKa = 8.51 PDD112 pKa = 3.22 HH113 pKa = 6.3 HH114 pKa = 6.67 FKK116 pKa = 11.29 YY117 pKa = 10.15 FMSMEE122 pKa = 4.11 RR123 pKa = 11.84 GEE125 pKa = 4.37 RR126 pKa = 11.84 TNRR129 pKa = 11.84 LHH131 pKa = 5.94 FHH133 pKa = 6.79 VILFSQIALSLSDD146 pKa = 3.46 GKK148 pKa = 10.83 FINGFMRR155 pKa = 11.84 YY156 pKa = 8.16 KK157 pKa = 10.52 AKK159 pKa = 10.43 EE160 pKa = 3.87 IQSTWNLGVNNEE172 pKa = 3.88 VSEE175 pKa = 4.5 FEE177 pKa = 4.43 CACAKK182 pKa = 10.28 YY183 pKa = 9.28 VAKK186 pKa = 9.42 YY187 pKa = 7.8 TSKK190 pKa = 10.9 NGLVRR195 pKa = 11.84 MSRR198 pKa = 11.84 NLGKK202 pKa = 10.49 SYY204 pKa = 10.96 VLSHH208 pKa = 6.73 ASEE211 pKa = 3.95 IVADD215 pKa = 3.86 NFKK218 pKa = 11.09 VYY220 pKa = 11.19 GDD222 pKa = 3.56 FSDD225 pKa = 4.56 RR226 pKa = 11.84 NRR228 pKa = 11.84 YY229 pKa = 7.23 WVNLPSQCALWLLDD243 pKa = 4.02 FYY245 pKa = 11.25 EE246 pKa = 5.12 SNVLSYY252 pKa = 10.96 KK253 pKa = 10.53 EE254 pKa = 3.96 SLQVLSHH261 pKa = 6.69 LKK263 pKa = 10.16 QIYY266 pKa = 9.66 SMKK269 pKa = 10.08 NRR271 pKa = 11.84 CSTCEE276 pKa = 4.31 DD277 pKa = 3.44 IEE279 pKa = 4.69 VIDD282 pKa = 4.47 TIYY285 pKa = 10.93 DD286 pKa = 3.54 EE287 pKa = 4.5 KK288 pKa = 11.12 NKK290 pKa = 10.08 KK291 pKa = 10.12 RR292 pKa = 11.84 GVKK295 pKa = 10.14 GCLL298 pKa = 3.1
Molecular weight: 34.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.789
IPC2_protein 7.951
IPC_protein 7.775
Toseland 7.41
ProMoST 8.346
Dawson 8.492
Bjellqvist 8.829
Wikipedia 8.434
Rodwell 8.521
Grimsley 7.41
Solomon 8.565
Lehninger 8.58
Nozaki 8.99
DTASelect 8.565
Thurlkill 8.609
EMBOSS 8.682
Sillero 8.931
Patrickios 3.961
IPC_peptide 8.565
IPC2_peptide 7.819
IPC2.peptide.svr19 8.008
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1378
88
559
229.7
25.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.894 ± 0.897
1.742 ± 0.578
6.531 ± 0.632
4.862 ± 0.411
5.007 ± 0.44
5.806 ± 1.015
1.742 ± 0.416
6.386 ± 1.296
6.313 ± 0.682
7.983 ± 0.727
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.83 ± 0.281
5.66 ± 0.752
3.846 ± 0.769
3.701 ± 0.373
3.846 ± 0.643
9.579 ± 0.761
5.443 ± 0.844
5.66 ± 0.701
1.451 ± 0.308
4.717 ± 0.759
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here