Escherichia phage RB16
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 270 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9ICH8|D9ICH8_9CAUD Conserved hypothetical phage protein OS=Escherichia phage RB16 OX=2681599 GN=RB16p117 PE=4 SV=1
MM1 pKa = 7.76 IYY3 pKa = 10.33 VSSYY7 pKa = 8.94 SADD10 pKa = 3.51 YY11 pKa = 10.39 EE12 pKa = 4.15 FTEE15 pKa = 4.26 IVHH18 pKa = 6.01 VSEE21 pKa = 5.09 SLQNCIDD28 pKa = 3.93 ALNKK32 pKa = 9.78 VDD34 pKa = 3.83 NFPYY38 pKa = 10.6 LGDD41 pKa = 4.49 NITIDD46 pKa = 2.83 VWEE49 pKa = 4.15 NEE51 pKa = 3.46 KK52 pKa = 10.74 RR53 pKa = 11.84 IAKK56 pKa = 9.99 SSTYY60 pKa = 10.43 GYY62 pKa = 10.18 RR63 pKa = 11.84 DD64 pKa = 3.04 VWDD67 pKa = 4.7 EE68 pKa = 3.71 EE69 pKa = 4.25 SQQYY73 pKa = 10.24 KK74 pKa = 9.93 EE75 pKa = 4.0 IPVTFEE81 pKa = 3.99 EE82 pKa = 4.5 IMANMRR88 pKa = 11.84 QVNEE92 pKa = 4.12 GG93 pKa = 3.21
Molecular weight: 10.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.918
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.935
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.215
Wikipedia 3.935
Rodwell 3.935
Grimsley 3.846
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.304
Thurlkill 3.961
EMBOSS 3.948
Sillero 4.215
Patrickios 1.939
IPC_peptide 4.05
IPC2_peptide 4.202
IPC2.peptide.svr19 4.114
Protein with the highest isoelectric point:
>tr|D9ICW9|D9ICW9_9CAUD Long tail fiber protein p37 OS=Escherichia phage RB16 OX=2681599 GN=36 PE=3 SV=1
MM1 pKa = 7.42 FPLKK5 pKa = 10.73 ARR7 pKa = 11.84 IRR9 pKa = 11.84 NKK11 pKa = 10.02 TGTQEE16 pKa = 3.4 WMEE19 pKa = 4.31 CLRR22 pKa = 11.84 EE23 pKa = 4.06 TKK25 pKa = 9.87 HH26 pKa = 6.66 YY27 pKa = 10.32 YY28 pKa = 9.38 ISQAVGYY35 pKa = 9.36 DD36 pKa = 3.38 GKK38 pKa = 10.6 NIRR41 pKa = 11.84 AKK43 pKa = 8.27 RR44 pKa = 11.84 TCWRR48 pKa = 11.84 SMIRR52 pKa = 11.84 RR53 pKa = 11.84 SHH55 pKa = 4.85 TPKK58 pKa = 10.65 LIKK61 pKa = 9.88 IWW63 pKa = 3.52
Molecular weight: 7.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.853
IPC_protein 10.496
Toseland 10.774
ProMoST 10.409
Dawson 10.862
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.228
Grimsley 10.906
Solomon 10.965
Lehninger 10.935
Nozaki 10.76
DTASelect 10.54
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.979
IPC_peptide 10.965
IPC2_peptide 9.545
IPC2.peptide.svr19 8.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
270
0
270
56230
20
1219
208.3
23.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.875 ± 0.185
1.17 ± 0.061
6.569 ± 0.094
6.824 ± 0.185
4.105 ± 0.107
6.308 ± 0.181
1.839 ± 0.084
6.534 ± 0.119
7.37 ± 0.234
7.539 ± 0.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.904 ± 0.07
5.486 ± 0.119
3.594 ± 0.091
3.347 ± 0.092
4.827 ± 0.124
5.867 ± 0.119
5.999 ± 0.243
7.123 ± 0.131
1.352 ± 0.058
4.37 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here