Listeria phage PSA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Psavirus; Listeria virus PSA

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8W602|Q8W602_9CAUD Gp7 protein OS=Listeria phage PSA OX=171618 PE=4 SV=1
MM1 pKa = 7.67NEE3 pKa = 3.68ATKK6 pKa = 10.73EE7 pKa = 3.99RR8 pKa = 11.84ITKK11 pKa = 9.99LNEE14 pKa = 3.5YY15 pKa = 10.17LITKK19 pKa = 10.24LSEE22 pKa = 3.97NTGLNVYY29 pKa = 9.68QDD31 pKa = 3.62NVSEE35 pKa = 4.41TEE37 pKa = 4.28LNEE40 pKa = 4.06EE41 pKa = 4.07MNGVYY46 pKa = 10.1DD47 pKa = 4.28YY48 pKa = 10.92IIFSTGGFSKK58 pKa = 10.86SDD60 pKa = 3.17TSQFTLIQEE69 pKa = 4.23VTVQVATEE77 pKa = 4.08NTSDD81 pKa = 5.02LDD83 pKa = 3.68GLQIDD88 pKa = 5.12IITSLEE94 pKa = 4.16GTGGAGPYY102 pKa = 10.32YY103 pKa = 10.27FLNSTKK109 pKa = 10.74GQVQKK114 pKa = 10.75GQQDD118 pKa = 4.3GYY120 pKa = 11.48VDD122 pKa = 3.73ALEE125 pKa = 5.4LIFTRR130 pKa = 11.84SFKK133 pKa = 10.54YY134 pKa = 10.36VCC136 pKa = 4.32

Molecular weight:
15.15 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8W5X5|Q8W5X5_9CAUD Gp32 protein OS=Listeria phage PSA OX=171618 PE=4 SV=1
MM1 pKa = 7.32FFLGKK6 pKa = 10.29YY7 pKa = 7.32YY8 pKa = 10.0WHH10 pKa = 6.93VSRR13 pKa = 11.84LGGKK17 pKa = 6.75PTEE20 pKa = 3.81IRR22 pKa = 11.84HH23 pKa = 5.37YY24 pKa = 10.6KK25 pKa = 10.26HH26 pKa = 5.13ITKK29 pKa = 9.48MYY31 pKa = 10.53KK32 pKa = 10.29FILRR36 pKa = 11.84NPAMFKK42 pKa = 10.89DD43 pKa = 3.79KK44 pKa = 10.4TLTIYY49 pKa = 11.07DD50 pKa = 3.48HH51 pKa = 7.07AKK53 pKa = 9.8PVTNMTFNEE62 pKa = 3.72IKK64 pKa = 10.7YY65 pKa = 9.87RR66 pKa = 11.84ASLNLCEE73 pKa = 4.24TVEE76 pKa = 4.09RR77 pKa = 11.84KK78 pKa = 9.52YY79 pKa = 11.5VLGLKK84 pKa = 9.69QRR86 pKa = 11.84LTEE89 pKa = 4.07EE90 pKa = 3.78QKK92 pKa = 10.49EE93 pKa = 4.1ARR95 pKa = 11.84KK96 pKa = 9.79

Molecular weight:
11.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

2

59

11948

48

1026

202.5

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.763 ± 0.494

0.879 ± 0.123

6.252 ± 0.195

8.093 ± 0.344

4.31 ± 0.217

5.608 ± 0.283

1.632 ± 0.196

7.189 ± 0.208

8.83 ± 0.36

8.378 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.114

6.369 ± 0.237

2.528 ± 0.205

3.473 ± 0.228

3.398 ± 0.201

5.708 ± 0.214

6.068 ± 0.301

6.403 ± 0.279

1.406 ± 0.142

4.001 ± 0.287

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski