Listeria phage PSA
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8W602|Q8W602_9CAUD Gp7 protein OS=Listeria phage PSA OX=171618 PE=4 SV=1
MM1 pKa = 7.67 NEE3 pKa = 3.68 ATKK6 pKa = 10.73 EE7 pKa = 3.99 RR8 pKa = 11.84 ITKK11 pKa = 9.99 LNEE14 pKa = 3.5 YY15 pKa = 10.17 LITKK19 pKa = 10.24 LSEE22 pKa = 3.97 NTGLNVYY29 pKa = 9.68 QDD31 pKa = 3.62 NVSEE35 pKa = 4.41 TEE37 pKa = 4.28 LNEE40 pKa = 4.06 EE41 pKa = 4.07 MNGVYY46 pKa = 10.1 DD47 pKa = 4.28 YY48 pKa = 10.92 IIFSTGGFSKK58 pKa = 10.86 SDD60 pKa = 3.17 TSQFTLIQEE69 pKa = 4.23 VTVQVATEE77 pKa = 4.08 NTSDD81 pKa = 5.02 LDD83 pKa = 3.68 GLQIDD88 pKa = 5.12 IITSLEE94 pKa = 4.16 GTGGAGPYY102 pKa = 10.32 YY103 pKa = 10.27 FLNSTKK109 pKa = 10.74 GQVQKK114 pKa = 10.75 GQQDD118 pKa = 4.3 GYY120 pKa = 11.48 VDD122 pKa = 3.73 ALEE125 pKa = 5.4 LIFTRR130 pKa = 11.84 SFKK133 pKa = 10.54 YY134 pKa = 10.36 VCC136 pKa = 4.32
Molecular weight: 15.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.094
IPC2_protein 4.215
IPC_protein 4.113
Toseland 3.935
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.948
Rodwell 3.948
Grimsley 3.846
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.317
Thurlkill 3.961
EMBOSS 3.961
Sillero 4.215
Patrickios 2.905
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.137
Protein with the highest isoelectric point:
>tr|Q8W5X5|Q8W5X5_9CAUD Gp32 protein OS=Listeria phage PSA OX=171618 PE=4 SV=1
MM1 pKa = 7.32 FFLGKK6 pKa = 10.29 YY7 pKa = 7.32 YY8 pKa = 10.0 WHH10 pKa = 6.93 VSRR13 pKa = 11.84 LGGKK17 pKa = 6.75 PTEE20 pKa = 3.81 IRR22 pKa = 11.84 HH23 pKa = 5.37 YY24 pKa = 10.6 KK25 pKa = 10.26 HH26 pKa = 5.13 ITKK29 pKa = 9.48 MYY31 pKa = 10.53 KK32 pKa = 10.29 FILRR36 pKa = 11.84 NPAMFKK42 pKa = 10.89 DD43 pKa = 3.79 KK44 pKa = 10.4 TLTIYY49 pKa = 11.07 DD50 pKa = 3.48 HH51 pKa = 7.07 AKK53 pKa = 9.8 PVTNMTFNEE62 pKa = 3.72 IKK64 pKa = 10.7 YY65 pKa = 9.87 RR66 pKa = 11.84 ASLNLCEE73 pKa = 4.24 TVEE76 pKa = 4.09 RR77 pKa = 11.84 KK78 pKa = 9.52 YY79 pKa = 11.5 VLGLKK84 pKa = 9.69 QRR86 pKa = 11.84 LTEE89 pKa = 4.07 EE90 pKa = 3.78 QKK92 pKa = 10.49 EE93 pKa = 4.1 ARR95 pKa = 11.84 KK96 pKa = 9.79
Molecular weight: 11.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.443
IPC_protein 9.414
Toseland 10.058
ProMoST 9.78
Dawson 10.262
Bjellqvist 9.911
Wikipedia 10.423
Rodwell 10.774
Grimsley 10.335
Solomon 10.277
Lehninger 10.248
Nozaki 10.043
DTASelect 9.911
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.175
Patrickios 10.423
IPC_peptide 10.277
IPC2_peptide 8.492
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
2
59
11948
48
1026
202.5
23.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.763 ± 0.494
0.879 ± 0.123
6.252 ± 0.195
8.093 ± 0.344
4.31 ± 0.217
5.608 ± 0.283
1.632 ± 0.196
7.189 ± 0.208
8.83 ± 0.36
8.378 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.114
6.369 ± 0.237
2.528 ± 0.205
3.473 ± 0.228
3.398 ± 0.201
5.708 ± 0.214
6.068 ± 0.301
6.403 ± 0.279
1.406 ± 0.142
4.001 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here