Amino acid dipepetide frequency for Clostridium phage phiMMP04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.202AlaAla: 2.202 ± 0.561
0.771AlaCys: 0.771 ± 0.25
2.532AlaAsp: 2.532 ± 0.477
4.294AlaGlu: 4.294 ± 0.667
1.872AlaPhe: 1.872 ± 0.296
3.083AlaGly: 3.083 ± 0.694
0.881AlaHis: 0.881 ± 0.253
4.954AlaIle: 4.954 ± 0.635
4.954AlaLys: 4.954 ± 0.812
4.294AlaLeu: 4.294 ± 0.645
1.541AlaMet: 1.541 ± 0.326
3.193AlaAsn: 3.193 ± 0.481
1.431AlaPro: 1.431 ± 0.472
1.651AlaGln: 1.651 ± 0.357
1.211AlaArg: 1.211 ± 0.452
4.184AlaSer: 4.184 ± 0.904
3.633AlaThr: 3.633 ± 0.738
3.083AlaVal: 3.083 ± 0.701
0.771AlaTrp: 0.771 ± 0.307
2.202AlaTyr: 2.202 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.33CysAla: 0.33 ± 0.2
0.22CysCys: 0.22 ± 0.145
0.661CysAsp: 0.661 ± 0.265
0.661CysGlu: 0.661 ± 0.173
0.33CysPhe: 0.33 ± 0.169
0.771CysGly: 0.771 ± 0.332
0.22CysHis: 0.22 ± 0.152
0.881CysIle: 0.881 ± 0.294
1.321CysLys: 1.321 ± 0.379
0.44CysLeu: 0.44 ± 0.191
0.22CysMet: 0.22 ± 0.11
0.881CysAsn: 0.881 ± 0.279
0.44CysPro: 0.44 ± 0.193
0.11CysGln: 0.11 ± 0.101
0.55CysArg: 0.55 ± 0.237
0.661CysSer: 0.661 ± 0.256
0.55CysThr: 0.55 ± 0.198
0.661CysVal: 0.661 ± 0.28
0.11CysTrp: 0.11 ± 0.103
0.33CysTyr: 0.33 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
2.862AspAla: 2.862 ± 0.552
0.881AspCys: 0.881 ± 0.296
2.973AspAsp: 2.973 ± 0.548
5.725AspGlu: 5.725 ± 0.872
2.312AspPhe: 2.312 ± 0.39
3.413AspGly: 3.413 ± 0.675
0.0AspHis: 0.0 ± 0.0
7.266AspIle: 7.266 ± 0.89
5.835AspLys: 5.835 ± 0.757
6.055AspLeu: 6.055 ± 0.797
1.431AspMet: 1.431 ± 0.321
3.193AspAsn: 3.193 ± 0.648
0.771AspPro: 0.771 ± 0.262
0.771AspGln: 0.771 ± 0.243
2.092AspArg: 2.092 ± 0.465
3.853AspSer: 3.853 ± 0.594
2.312AspThr: 2.312 ± 0.525
3.633AspVal: 3.633 ± 0.776
0.22AspTrp: 0.22 ± 0.148
2.422AspTyr: 2.422 ± 0.397
0.0AspXaa: 0.0 ± 0.0
Glu
5.175GluAla: 5.175 ± 0.847
1.431GluCys: 1.431 ± 0.411
4.624GluAsp: 4.624 ± 0.644
8.587GluGlu: 8.587 ± 1.414
4.734GluPhe: 4.734 ± 0.673
4.294GluGly: 4.294 ± 0.728
1.541GluHis: 1.541 ± 0.329
9.028GluIle: 9.028 ± 1.212
10.019GluLys: 10.019 ± 1.133
10.019GluLeu: 10.019 ± 1.157
2.422GluMet: 2.422 ± 0.513
6.055GluAsn: 6.055 ± 0.85
0.991GluPro: 0.991 ± 0.25
2.202GluGln: 2.202 ± 0.311
2.532GluArg: 2.532 ± 0.506
3.413GluSer: 3.413 ± 0.756
5.064GluThr: 5.064 ± 0.668
5.835GluVal: 5.835 ± 0.845
0.881GluTrp: 0.881 ± 0.272
3.523GluTyr: 3.523 ± 0.698
0.0GluXaa: 0.0 ± 0.0
Phe
1.101PheAla: 1.101 ± 0.396
0.44PheCys: 0.44 ± 0.209
2.422PheAsp: 2.422 ± 0.564
3.303PheGlu: 3.303 ± 0.503
1.321PhePhe: 1.321 ± 0.334
3.083PheGly: 3.083 ± 0.455
0.44PheHis: 0.44 ± 0.172
3.963PheIle: 3.963 ± 0.625
4.734PheLys: 4.734 ± 0.561
3.303PheLeu: 3.303 ± 0.61
1.321PheMet: 1.321 ± 0.305
3.633PheAsn: 3.633 ± 0.612
0.991PhePro: 0.991 ± 0.507
0.881PheGln: 0.881 ± 0.277
1.541PheArg: 1.541 ± 0.343
1.762PheSer: 1.762 ± 0.553
1.982PheThr: 1.982 ± 0.383
1.872PheVal: 1.872 ± 0.407
0.11PheTrp: 0.11 ± 0.119
1.541PheTyr: 1.541 ± 0.394
0.0PheXaa: 0.0 ± 0.0
Gly
2.752GlyAla: 2.752 ± 0.683
0.881GlyCys: 0.881 ± 0.228
3.303GlyAsp: 3.303 ± 0.702
4.844GlyGlu: 4.844 ± 0.628
1.982GlyPhe: 1.982 ± 0.439
3.743GlyGly: 3.743 ± 0.655
0.33GlyHis: 0.33 ± 0.216
4.074GlyIle: 4.074 ± 0.799
7.266GlyLys: 7.266 ± 0.797
4.404GlyLeu: 4.404 ± 0.668
1.541GlyMet: 1.541 ± 0.676
3.633GlyAsn: 3.633 ± 0.517
0.11GlyPro: 0.11 ± 0.101
0.661GlyGln: 0.661 ± 0.189
1.541GlyArg: 1.541 ± 0.491
2.422GlySer: 2.422 ± 0.467
4.074GlyThr: 4.074 ± 0.783
3.743GlyVal: 3.743 ± 0.521
0.771GlyTrp: 0.771 ± 0.298
2.202GlyTyr: 2.202 ± 0.546
0.0GlyXaa: 0.0 ± 0.0
His
0.11HisAla: 0.11 ± 0.1
0.11HisCys: 0.11 ± 0.104
0.55HisAsp: 0.55 ± 0.229
0.771HisGlu: 0.771 ± 0.304
0.44HisPhe: 0.44 ± 0.202
0.44HisGly: 0.44 ± 0.181
0.22HisHis: 0.22 ± 0.158
0.771HisIle: 0.771 ± 0.228
1.211HisLys: 1.211 ± 0.316
1.101HisLeu: 1.101 ± 0.305
0.55HisMet: 0.55 ± 0.266
0.881HisAsn: 0.881 ± 0.315
0.0HisPro: 0.0 ± 0.0
0.55HisGln: 0.55 ± 0.229
0.33HisArg: 0.33 ± 0.181
0.771HisSer: 0.771 ± 0.316
0.55HisThr: 0.55 ± 0.235
0.771HisVal: 0.771 ± 0.293
0.0HisTrp: 0.0 ± 0.0
0.55HisTyr: 0.55 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
4.734IleAla: 4.734 ± 0.813
0.881IleCys: 0.881 ± 0.358
7.046IleAsp: 7.046 ± 0.823
8.918IleGlu: 8.918 ± 0.923
3.303IlePhe: 3.303 ± 0.554
4.954IleGly: 4.954 ± 0.875
1.321IleHis: 1.321 ± 0.327
7.927IleIle: 7.927 ± 0.906
9.799IleLys: 9.799 ± 0.956
8.147IleLeu: 8.147 ± 1.203
2.202IleMet: 2.202 ± 0.462
5.505IleAsn: 5.505 ± 0.952
2.973IlePro: 2.973 ± 0.447
2.422IleGln: 2.422 ± 0.365
3.413IleArg: 3.413 ± 0.604
6.165IleSer: 6.165 ± 0.889
4.404IleThr: 4.404 ± 0.608
5.285IleVal: 5.285 ± 0.818
0.33IleTrp: 0.33 ± 0.219
3.963IleTyr: 3.963 ± 0.757
0.0IleXaa: 0.0 ± 0.0
Lys
7.046LysAla: 7.046 ± 0.837
0.881LysCys: 0.881 ± 0.299
5.835LysAsp: 5.835 ± 0.707
11.89LysGlu: 11.89 ± 1.286
3.963LysPhe: 3.963 ± 0.7
4.074LysGly: 4.074 ± 0.68
1.651LysHis: 1.651 ± 0.395
10.899LysIle: 10.899 ± 0.781
10.019LysLys: 10.019 ± 1.323
10.459LysLeu: 10.459 ± 1.106
2.642LysMet: 2.642 ± 0.567
7.156LysAsn: 7.156 ± 0.727
2.642LysPro: 2.642 ± 0.484
2.862LysGln: 2.862 ± 0.701
3.083LysArg: 3.083 ± 0.593
5.285LysSer: 5.285 ± 0.852
6.386LysThr: 6.386 ± 0.792
5.725LysVal: 5.725 ± 0.775
0.881LysTrp: 0.881 ± 0.259
4.294LysTyr: 4.294 ± 0.616
0.0LysXaa: 0.0 ± 0.0
Leu
5.835LeuAla: 5.835 ± 1.068
0.661LeuCys: 0.661 ± 0.288
5.945LeuAsp: 5.945 ± 0.683
8.037LeuGlu: 8.037 ± 1.45
3.303LeuPhe: 3.303 ± 0.563
3.633LeuGly: 3.633 ± 0.6
0.771LeuHis: 0.771 ± 0.323
6.055LeuIle: 6.055 ± 0.741
10.569LeuLys: 10.569 ± 1.029
7.376LeuLeu: 7.376 ± 0.924
2.202LeuMet: 2.202 ± 0.486
5.505LeuAsn: 5.505 ± 0.913
1.211LeuPro: 1.211 ± 0.38
1.982LeuGln: 1.982 ± 0.487
4.404LeuArg: 4.404 ± 0.789
5.725LeuSer: 5.725 ± 0.752
3.743LeuThr: 3.743 ± 0.661
3.413LeuVal: 3.413 ± 0.544
0.661LeuTrp: 0.661 ± 0.309
3.303LeuTyr: 3.303 ± 0.581
0.0LeuXaa: 0.0 ± 0.0
Met
1.541MetAla: 1.541 ± 0.615
0.22MetCys: 0.22 ± 0.14
1.321MetAsp: 1.321 ± 0.264
2.642MetGlu: 2.642 ± 0.474
0.771MetPhe: 0.771 ± 0.214
1.431MetGly: 1.431 ± 0.423
0.11MetHis: 0.11 ± 0.087
2.422MetIle: 2.422 ± 0.532
2.312MetLys: 2.312 ± 0.438
1.762MetLeu: 1.762 ± 0.416
0.661MetMet: 0.661 ± 0.224
2.092MetAsn: 2.092 ± 0.472
0.55MetPro: 0.55 ± 0.255
0.44MetGln: 0.44 ± 0.146
0.881MetArg: 0.881 ± 0.45
1.541MetSer: 1.541 ± 0.389
1.541MetThr: 1.541 ± 0.325
1.101MetVal: 1.101 ± 0.434
0.11MetTrp: 0.11 ± 0.116
1.321MetTyr: 1.321 ± 0.31
0.0MetXaa: 0.0 ± 0.0
Asn
2.973AsnAla: 2.973 ± 0.604
0.55AsnCys: 0.55 ± 0.208
2.973AsnAsp: 2.973 ± 0.656
5.505AsnGlu: 5.505 ± 0.8
2.092AsnPhe: 2.092 ± 0.381
3.853AsnGly: 3.853 ± 0.787
0.33AsnHis: 0.33 ± 0.173
7.487AsnIle: 7.487 ± 0.937
6.936AsnLys: 6.936 ± 0.832
4.404AsnLeu: 4.404 ± 0.668
1.431AsnMet: 1.431 ± 0.386
4.514AsnAsn: 4.514 ± 0.739
1.762AsnPro: 1.762 ± 0.534
0.991AsnGln: 0.991 ± 0.39
2.752AsnArg: 2.752 ± 0.503
5.505AsnSer: 5.505 ± 0.744
3.523AsnThr: 3.523 ± 0.612
3.963AsnVal: 3.963 ± 0.603
0.661AsnTrp: 0.661 ± 0.23
2.752AsnTyr: 2.752 ± 0.704
0.0AsnXaa: 0.0 ± 0.0
Pro
1.431ProAla: 1.431 ± 0.318
0.22ProCys: 0.22 ± 0.16
0.881ProAsp: 0.881 ± 0.242
0.661ProGlu: 0.661 ± 0.201
1.101ProPhe: 1.101 ± 0.313
0.991ProGly: 0.991 ± 0.248
0.44ProHis: 0.44 ± 0.186
2.312ProIle: 2.312 ± 0.538
1.431ProLys: 1.431 ± 0.333
1.872ProLeu: 1.872 ± 0.452
0.44ProMet: 0.44 ± 0.202
1.211ProAsn: 1.211 ± 0.332
0.0ProPro: 0.0 ± 0.0
0.661ProGln: 0.661 ± 0.246
0.661ProArg: 0.661 ± 0.216
2.532ProSer: 2.532 ± 0.553
1.211ProThr: 1.211 ± 0.278
1.431ProVal: 1.431 ± 0.358
0.33ProTrp: 0.33 ± 0.224
1.101ProTyr: 1.101 ± 0.352
0.0ProXaa: 0.0 ± 0.0
Gln
2.092GlnAla: 2.092 ± 0.492
0.0GlnCys: 0.0 ± 0.0
1.211GlnAsp: 1.211 ± 0.26
2.202GlnGlu: 2.202 ± 0.512
0.881GlnPhe: 0.881 ± 0.278
0.991GlnGly: 0.991 ± 0.354
0.11GlnHis: 0.11 ± 0.116
2.422GlnIle: 2.422 ± 0.46
2.312GlnLys: 2.312 ± 0.535
1.762GlnLeu: 1.762 ± 0.387
0.55GlnMet: 0.55 ± 0.268
0.771GlnAsn: 0.771 ± 0.293
0.661GlnPro: 0.661 ± 0.292
1.101GlnGln: 1.101 ± 0.433
0.881GlnArg: 0.881 ± 0.312
1.101GlnSer: 1.101 ± 0.377
1.541GlnThr: 1.541 ± 0.359
1.321GlnVal: 1.321 ± 0.303
0.11GlnTrp: 0.11 ± 0.094
1.431GlnTyr: 1.431 ± 0.307
0.0GlnXaa: 0.0 ± 0.0
Arg
1.762ArgAla: 1.762 ± 0.413
0.22ArgCys: 0.22 ± 0.157
1.211ArgAsp: 1.211 ± 0.4
4.624ArgGlu: 4.624 ± 0.692
1.321ArgPhe: 1.321 ± 0.323
2.752ArgGly: 2.752 ± 0.384
0.661ArgHis: 0.661 ± 0.258
3.853ArgIle: 3.853 ± 0.678
3.853ArgLys: 3.853 ± 0.738
2.202ArgLeu: 2.202 ± 0.428
1.321ArgMet: 1.321 ± 0.404
1.762ArgAsn: 1.762 ± 0.395
0.55ArgPro: 0.55 ± 0.216
1.321ArgGln: 1.321 ± 0.38
2.422ArgArg: 2.422 ± 0.554
1.872ArgSer: 1.872 ± 0.456
2.092ArgThr: 2.092 ± 0.51
1.101ArgVal: 1.101 ± 0.321
0.55ArgTrp: 0.55 ± 0.208
1.541ArgTyr: 1.541 ± 0.367
0.0ArgXaa: 0.0 ± 0.0
Ser
2.642SerAla: 2.642 ± 0.6
0.55SerCys: 0.55 ± 0.242
2.862SerAsp: 2.862 ± 0.575
5.175SerGlu: 5.175 ± 0.804
3.963SerPhe: 3.963 ± 0.563
3.083SerGly: 3.083 ± 0.572
0.33SerHis: 0.33 ± 0.191
5.725SerIle: 5.725 ± 0.923
7.266SerLys: 7.266 ± 0.842
4.624SerLeu: 4.624 ± 0.597
1.541SerMet: 1.541 ± 0.406
4.844SerAsn: 4.844 ± 0.801
1.431SerPro: 1.431 ± 0.362
0.991SerGln: 0.991 ± 0.288
2.202SerArg: 2.202 ± 0.456
4.294SerSer: 4.294 ± 0.834
2.973SerThr: 2.973 ± 0.518
3.083SerVal: 3.083 ± 0.58
0.771SerTrp: 0.771 ± 0.259
2.752SerTyr: 2.752 ± 0.528
0.0SerXaa: 0.0 ± 0.0
Thr
3.413ThrAla: 3.413 ± 0.703
0.33ThrCys: 0.33 ± 0.203
3.743ThrAsp: 3.743 ± 0.681
6.496ThrGlu: 6.496 ± 0.9
1.651ThrPhe: 1.651 ± 0.412
3.963ThrGly: 3.963 ± 0.718
0.55ThrHis: 0.55 ± 0.238
5.395ThrIle: 5.395 ± 0.769
4.954ThrLys: 4.954 ± 0.635
4.294ThrLeu: 4.294 ± 0.672
0.55ThrMet: 0.55 ± 0.264
3.193ThrAsn: 3.193 ± 0.544
1.211ThrPro: 1.211 ± 0.28
1.431ThrGln: 1.431 ± 0.341
2.092ThrArg: 2.092 ± 0.421
3.853ThrSer: 3.853 ± 0.784
3.743ThrThr: 3.743 ± 0.742
3.413ThrVal: 3.413 ± 0.578
0.661ThrTrp: 0.661 ± 0.252
1.431ThrTyr: 1.431 ± 0.275
0.0ThrXaa: 0.0 ± 0.0
Val
3.193ValAla: 3.193 ± 0.634
0.44ValCys: 0.44 ± 0.246
3.633ValAsp: 3.633 ± 0.606
3.853ValGlu: 3.853 ± 0.835
2.422ValPhe: 2.422 ± 0.383
3.523ValGly: 3.523 ± 0.76
0.11ValHis: 0.11 ± 0.105
3.743ValIle: 3.743 ± 0.758
7.597ValLys: 7.597 ± 0.688
4.844ValLeu: 4.844 ± 0.779
1.101ValMet: 1.101 ± 0.269
3.193ValAsn: 3.193 ± 0.64
1.762ValPro: 1.762 ± 0.312
1.651ValGln: 1.651 ± 0.404
2.092ValArg: 2.092 ± 0.485
3.083ValSer: 3.083 ± 0.709
3.303ValThr: 3.303 ± 0.606
5.725ValVal: 5.725 ± 0.824
0.33ValTrp: 0.33 ± 0.19
2.532ValTyr: 2.532 ± 0.506
0.0ValXaa: 0.0 ± 0.0
Trp
0.44TrpAla: 0.44 ± 0.183
0.33TrpCys: 0.33 ± 0.188
0.991TrpAsp: 0.991 ± 0.292
0.44TrpGlu: 0.44 ± 0.212
0.33TrpPhe: 0.33 ± 0.171
0.55TrpGly: 0.55 ± 0.234
0.22TrpHis: 0.22 ± 0.125
0.771TrpIle: 0.771 ± 0.343
0.991TrpLys: 0.991 ± 0.287
0.661TrpLeu: 0.661 ± 0.209
0.22TrpMet: 0.22 ± 0.175
0.44TrpAsn: 0.44 ± 0.217
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.22TrpArg: 0.22 ± 0.123
0.44TrpSer: 0.44 ± 0.207
0.44TrpThr: 0.44 ± 0.194
0.991TrpVal: 0.991 ± 0.354
0.11TrpTrp: 0.11 ± 0.11
0.22TrpTyr: 0.22 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.101TyrAla: 1.101 ± 0.266
0.33TyrCys: 0.33 ± 0.175
3.303TyrAsp: 3.303 ± 0.732
3.743TyrGlu: 3.743 ± 0.586
1.651TyrPhe: 1.651 ± 0.498
1.651TyrGly: 1.651 ± 0.504
0.44TyrHis: 0.44 ± 0.226
3.743TyrIle: 3.743 ± 0.571
4.404TyrLys: 4.404 ± 0.61
2.312TyrLeu: 2.312 ± 0.474
0.771TyrMet: 0.771 ± 0.32
3.303TyrAsn: 3.303 ± 0.622
1.431TyrPro: 1.431 ± 0.359
0.771TyrGln: 0.771 ± 0.244
2.092TyrArg: 2.092 ± 0.529
2.642TyrSer: 2.642 ± 0.724
3.303TyrThr: 3.303 ± 0.583
1.982TyrVal: 1.982 ± 0.459
0.44TyrTrp: 0.44 ± 0.2
1.211TyrTyr: 1.211 ± 0.328
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (9084 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski